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pcDNA3.1-pA83-HA-TAZ

Expression clone of mouse TAZ

Catalog number RDB12193
Resource name pcDNA3.1-pA83-HA-TAZ
Clone info. Expression clone of mouse TAZ. cDNA fragment 281 to 1,468 of NM_133784.3 (CDS, 281..1468) is cloned.
See Fig. 4A of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Two instead of one T7 promoter sequences are appeared.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Wwtr1|TAZ
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Wwtr1 (NCBI Gene 97064) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12193 pcDNA3.1-pA83-HA-TAZ DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12193_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12193_A6Aja.seq
>12193_12193_A6Aj_CMV-Forward_D03_12.ab1
    1 ACTTTTAACT TCCGCCCCAT TGACGCAAAA TGGGCGGTAG GCGTGGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTTGGTACC GAGCTCGGAT
  181 CCACTAGTCC AGTGTGGTGG AATTGTAATA CGACTCACTA TAGGGAGACC CAAGCTTGCC
  241 GCCACCATGT ACCCATACGA CGTTCCAGAT TACGCTGGTA CCATGAATCC GTCCTCGGTG
  301 CCCCATCCGC TCCCGCCGCC AGGGCAGCAA GTCATCCACG TCACGCAGGA CCTGGACACC
  361 GACCTCGAAG CCCTCTTCAA CTCTGTCATG AACCCCAAGC CCAGCTCATG GCGGAAAAAG
  421 ATCCTCCCGG AGTCCTTCTT TAAGGAGCCC GATTCCGGCT CGCACTCGCG CCAATCCAGC
  481 ACAGACTCAT CAGGCGGCCA CCCGGGGCCT CGACTAGCTG GCGGCGCGCA GCACGTCCGC
  541 TCGCACTCGT CGCCCGCATC CCTGCAGCTG GGCACCGGTG CGGGAGCCGC TGGAGGCCCT
  601 GCACAGCAGC ATGCACATCT CCGCCAGCAG TCCTATGACG TGACCGACGA GCTGCCGTTG
  661 CCCCCCGGGT GGGAGATGAC CTTCACGGCC ACTGGCCAGA GATACTTCCT TAATCACATA
  721 GAGAAAATCA CCACATGGCA AGACCCCAGG AAGGTGATGA ATCAGCCTCT GAATCATGTG
  781 AACCTCCACC CGTCCATCAC TTCCACCTCG GTGCCACAGA GGTCCATGGC AGTGTCCCAG
  841 CCGAATCTCG CAATGAATCA CCAAACACCA GCAAGTCGTG GCCACTAGCC TGAGTCCACA
  901 GGAACCACCC GACTCAGAAC CAACCCACAG GGCTCATGAG TGTGCCCAAT GCACTGACCA
  961 CTCAGCAGCA GCAGCAGCAG AAACTGCGGC TTCAGAGGAT CCAGGATGGA GAGAGAGAAG
 1021 GATTAGATGC GTCAAGAGGA GGCTCATGGA GCAGAAGCTG CCCCTCTGCC CGACAGCCTC
 1081 CCAATGGAAA ACCGAGACCA ATGCCCTGTC CAACCACGGC TTGCATGGAG CCACAGAATT
1141 ATGTGAAAGA ATCTTGTTTC TACACCCA
//
Primer: GNP_primer#10 RDB12193_A6Ajb.seq
>12193_12193_A6Aj_GNPprimer#10_E03_15.ab1
    1 CTATTTGGTG CAAATTCATT TAAAAGATAA CGTTTACAGG TTCTTTGGTG CCCATAGTCG
   61 AGCGGCCGCC ACTGTGCTGG ATATCTGCAG AATTTAGAAG GCACAGTCGA GGCTGATCAG
  121 CGAGCTCTAG CATTTAGGTG ACACTATAGA ATAGGGCCCT CTAGATGCAT GCTCGAGCGG
  181 CCGCCAGTGT GATGGATATC TGCAGAATTC TTACAGCCAG GTTAGAAAGG GCTCGCTTTT
  241 GTTCAGAGCA GACTCTACAT CATTGAAGAG AGGGATCAGA TCTTCAGACT CCAAAGTCCC
  301 GAGGTCAACA TTTGTTCCTG GAAGGCAGTC CAGGAAATCA GGGAAGCGGG TCTGCTGGGG
  361 ATTGACGGTC ATGGGTGTCT GACCGGAATT TTCACCTGTA TCCATCTCGT CCATGTTGCT
  421 GAGGAAGTCT TCTGGAGTTG TGGGGACACT GTAGCACCCT AACCCCAGGC CACTGTCTGT
  481 GCTCTGCTCC CGTGAATGAT AGGGCCCTCC ATTGAGGAAA GGATCTGAGC TACTGTTGGT
  541 GACAGATCTC ATATCTGTGC TCATGGCAGG CGTGTTGACA GGGGCCATGG TCTCGGTTTC
  601 CATGGGGAGC TGTCGGCAGA GGGCAGCTTC CTGCCTCATG AGCTCCTCTT GACGCATCCT
  661 AATCCTCTCT CTCTCCATCT GGATCCTCTG AAGCCGCAGT TTCTGCTGCT GCTGCTGCTG
  721 AGTGGTCAGT GCATTGGGCA ACACTCATGA GCCCTGTGGG TTGGTTCTGA GTCGGGTGGT
  781 TCTGTGGACT CAGGCTAGTG GCCACGACTT GCTGGGTGTT GGTGATTCAT TGCGAGATTC
  841 GGCTGGGACA CTGCCATGGA CCTCTGTGGC ACCGAGGTGG AAAGTGAATG GACGGGTGGA
  901 GGTTTCACAT GATTCAGAGG CTGATTCATC ACCCTTCCCT GGGGGTCTTG CCCATGTGGG
  961 TGATTTTTCT CTATGTGATT AAGGAAGTTA TCTCTGGGCC AAGTGGGCCG TGAAGGTCAT
 1021 CTTCCCCACC CGGGGGGGCA ACGGCAGCTC GTTCCGGTCA CGTCATAGAA CTTGCTTGGC
 1081 GGGAGAATTG TGCAATGCTG CCTGTGCAAG GGCCTCAGCG GACTTCCGGT CAACGGTGTC
1141 CAAACCTGCC AGGGAATGGC GGGCGCAAGC AAGAGTGGCG AAAGC
//
Primer: BGH_rev2 RDB12193_A6Ajc.seq
>12193_12193_A6Aj_BGH_rev2_B04_04.ab1
    1 TATGGGTAGA GCACGGGGGA GGGGCAACAA CAGATGGCTG GCAACTAGAA GGCACAGTCG
   61 AGGCTGATCA GCGGGTTTAA ACTCAATGGT GATGGTGATG ATGACCGGTA TGCATATTCA
  121 GATCCTCTTC TGAGATGAGT TTTTGTTCGA AGGGCCCTCT AGACTCGAGG TACGCCACAG
  181 CGTATGATGC GTATTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  241 TTTTTTTTTT TTTTTTTTTT TTTTCTTTCC CAAATTTTTT TTTGGGCCCT TTCAATTTAA
  301 AAAAAAAACT TTTACGGGTT TTTTGGGCCC CAAAGCCAAG CCGCCCCCCC TGTGGGGGAT
  361 TTTTCCAAAA TTTAAAAGGC CCAGTCGGGG GTTTTCCCCG GCCTTTAACT TTTGGGGGAC
  421 CCTTAAAAAA GGGGCCCCTT AAATCCTTCC CCCACCCGCC CCCCGGGGGA GGAATTTTTC
  481 CAAAATTTTT CCCCCCCGTT TAAAAAGGGC CTCTTTTTTT TCAAAACAAA CTCTCCTTTT
  541 TTAAAGAAGG GGGTTAATTT TTCAAACCCC AAAGCCCCGG GGGCAAATTT TTTTCCTGGA
  601 AGGGCTCCCA AAAAAACAGG GAACCGGGTC TTCGGGGGAT TAACGGCCAT GGGTTTTTAC
  661 CCCAAATTTT CCCCGGTTCC CCTCCCCCCC ATGTTGGGGA AAAAATCTTT TGGGTTGGGG
  721 GGGAACTTTT ACCCCCCTAA CCCCGGGCCA TTTTTTTGTT TCTCCCCCCG AAAAAAAAAA
  781 AGGGCCCCCC CTTTGGGGAA AAGGGTTTTG ACCCCCCTTT TGGGGGAAAA AAAACCAAAA
  841 ACTGGGCCCC ATGGGCGGGG TTTTTTTAAA GGGGGCCCAG GGCCCCCGGT TCCCAGGGGG
  901 GAGAATTTTC CCCAAAAGGG GAACCTTTCC CCCCCCCAAA AACCTCCTCT TTTGAACCCA
  961 CCCAAAAACT TTTTTTTTTT TAAATGGGGA CACCCCCGAA AACCCCCCAA TTTTTTCTCT
 1021 GCTGTCCGGT GGGGAAAAGA GGCCCAGGGC CTTTTGGGCG CACACCTTTA TAAGCCCCCC
 1081 GTGTGGGGGG TGGTGGTCTT CTAGAGATCT GCGGGGGGGT GTCTCTCGTG GAGACTACTC
 1141 AGAGCTCTGT AGTGTGGCCA CACCACATTT TTGGTGTGTT TGTGTGATAA TATATATCAT
1201 ATTAGCGCA
//
Primer: SV40pro_F RDB12193_A6Ajd.seq
>12193_12193_A6Aj_SV40pro_F_G03_21.ab1
    1 CCTGTCTGGC ATTCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
  121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
  661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAC CAAGCGACGC
  961 CCAACCCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG TGGGCTTCGG
 1021 AATCGTTTTC CGGACGCAGC TGGATGATCC TCCAGCGCGG GGAATCTCAA TGCCTGCAGT
1081 CCTCGCCACC CAACTTGTTA TGGCAGCCTA TATGGTTACC AACTAAGGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl