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pcDNA3.1-pA83-HA-TAZ

Expression clone of mouse TAZ

Catalog number RDB12193
Resource name pcDNA3.1-pA83-HA-TAZ
Clone info. Expression clone of mouse TAZ. cDNA fragment 281 to 1,468 of NM_133784.3 (CDS, 281..1468) is cloned.
Comment See Fig. 4A of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Two instead of one T7 promoter sequences are appeared.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Wwtr1|TAZ
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank
External Database
Mouse Wwtr1|TAZ
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
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          RDB12193 pcDNA3.1-pA83-HA-TAZ DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3.1-pA83-HA-TAZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12193).

          Reference section:

          Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12193_A6Ajp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro x2,insert 5'(HA,mTAZ 5')
          Sequence file: RDB12193_A6Aja.seq check
          >12193_12193_A6Aj_CMV-Forward_D03_12.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCGGTAN GCGTNNTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
            121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTTGGTACC GAGCTCGGAT
            181 CCACTAGTCC AGTGTGGTGG AATTGTAATA CGACTCACTA TAGGGAGACC CAAGCTTGCC
            241 GCCACCATGT ACCCATACGA CGTTCCAGAT TACGCTGGTA CCATGAATCC GTCCTCGGTG
            301 CCCCATCCGC TCCCGCCGCC AGGGCAGCAA GTCATCCACG TCACGCAGGA CCTGGACACC
            361 GACCTCGAAG CCCTCTTCAA CTCTGTCATG AACCCCAAGC CCAGCTCATG GCGGAAAAAG
            421 ATCCTCCCGG AGTCCTTCTT TAAGGAGCCC GATTCCGGCT CGCACTCGCG CCAATCCAGC
            481 ACAGACTCAT CAGGCGGCCN NCCGGGGCCT CGACTAGCTG GCGGCGCGCA GCACGTCCGC
            541 TCGCACTCGT CGCCCGCATC CCTGCAGCTG GGCACCGGTG CGGGAGCCGC TGGAGGCCCT
            601 GCACAGCAGC ATGCACATCT CCGCCAGCAG TCCTATGACG TGACCGACGA GCTGCCGTTG
            661 CCCCCCGGGT GGGAGATGAC CTTCACGGCC ACTGGCCAGA GATACTTCCT TAATCACATA
            721 GAGAAAATCA CNNNNNGGCA AGACCCCAGG AANGTGATGA ATCAGCCTCT GAATCATGTG
            781 AACCTCCACC CGTCCATCAC TTCCNCCTCG GTGCNNCAGA NGTCCATGGC AGTGTCCCAG
            841 CCGAATCTCG CANTGANTCA CCNAACNCCA GCNAGNCGTG GCNNCTANCC TGANTNCACN
            901 NNAACNNCCC GACNNANAAN NNACCCACAN GGCTCATNAG TGTGCCNNNN GCACNNNNCA
            961 CTCNGCAGCA GCAGCANCNN AAACTGNNGN TNCNNAGNAN NCANNANNNN NANANANNNN
          1021 GANTNNNTGC NTNNNGANGN NNNNNATNNN NNNNNNN
          //
          Primer: GNP Primer #10 (Pr0763)
          Region: TRF2 3’-UTR and polyA,insert 3' (mTAZ 3')
          Sequence file: RDB12193_A6Ajb.seq check
          >12193_12193_A6Aj_GNPprimer#10_E03_15.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAA CGTTTACNGG TTCTTTGGTG CCCATAGTCG
             61 AGCGGCCGCC ACTGTGCTGG ATATCTGCAG AATTTAGAAG GCACAGTCGA GGCTGATCAG
            121 CGAGCTCTAG CATTTAGGTG ACACTATAGA ATAGGGCCCT CTAGATGCAT GCTCGAGCGG
            181 CCGCCAGTGT GATGGATATC TGCAGAATTC TTACAGCCAG GTTAGAAAGG GCTCGCTTTT
            241 GTTCAGAGCA GACTCTACAT CATTGAAGAG AGGGATCAGA TCTTCAGACT CCAAAGTCCC
            301 GAGGTCAACA TTTGTTCCTG GAAGGCAGTC CANGAAATCA GGGAAGCGGG TCTGCTGGGG
            361 ATTGACGGTC ATGGGTGTCT GACCGGAATT TTCACCTGTA TCCATCTCGT CCATGTTGCT
            421 NANNAAGTCT TCTGGAGTTG TGGGGACACT GTAGCACCCT AACCCCAGGC CACTGTCTGT
            481 GCTCTGCTCC CGTGAATGAT AGGGCCCTCC ATTGAGGAAA GGATCTGAGC TACTGTTGGT
            541 GACAGATCTC ATATCTGTGC TCATGGCAGG CGTGTTGACA GGGGCCATGG TCTCGGTTTC
            601 CATGGGGAGC TGTCNNNNNA GGGCANCTTC CTGCCTCATG AGCTCCTCTT GACNCATCCT
            661 AATCCTCTCT CTCTCCATCT GGATCCTCTG AANCCNCNNT TTCTGCTGCT GCTGCTGCTG
            721 ANTGGTCAGT GCATTGGNCN NNNNTCNTGA GCCCTGTNGG TTGGTTCTGA GTCGGGTNGT
            781 TCTGTGGANT CAGGCNNNNG NCCACGACTT GCNNGGTGTT GGTGATTCAT TGCNAGATTC
            841 NGCTGGGACA CTGCCNTGGA CCTCTGTGGC ACCNANGNNN NAANTNNNNG NNCNGGTGNA
            901 GGTTNNACNT GATTCANANG NTNNNTNNTC ACCNTTNCNN NGGGNNNNNG NCNNNNNNGG
            961 NGNNTTTTCT CTATGTNANN NANNNANNNN TNNCTNGNCC NNGTNGNNNN NNNNNGNNNN
          1021 NNNNNCNNCN NNNGNGGGNN NNNNNNNN
          //
          Primer: bGH_rev2 (Pr0606)
          Region: TRF2 3’-UTR and polyA
          Sequence file: RDB12193_A6Ajc.seq check
          >12193_12193_A6Aj_BGH_rev2_B04_04.ab1
              1 NNNNNNNNNN NNNNGGNNGN NGGNNNNNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG
             61 AGGCTGATNN NNGGGTTTAA ACTCAATGGT GATGGTGATG ATGACCGGTA TGCATATTCA
            121 GATCCTCTTC TGAGATGAGT TTTTGTTCGA AGGGCCCTCT AGACTCGAGG TACGCCACAG
            181 CGTATGATGC GTATTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
          241 TTTTTTTTTT TTTTTTTTTN NNNNNNNNN
          //
          Primer: SV40pro_F
          Sequence file: RDB12193_A6Ajd.seq check
          >12193_12193_A6Aj_SV40pro_F_G03_21.ab1
              1 CCTGTCTGGC ATTCAGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
             61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT
            121 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
            181 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
            241 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
            301 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
            361 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
            421 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
            481 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
            541 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
            601 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC
            661 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
            721 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
            781 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
            841 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
            901 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAC CAAGCGACGC
            961 CCAACCCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG TGGGCTTCGG
           1021 AATCGTTTTC CGGACGCAGC TGGATGATCC TCCAGCGCGG GGAATCTCAA TGCCTGCAGT
          1081 CCTCGCCACC CAACTTGTTA TGGCAGCCTA TATGGTTACC AACTAAGGCA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl