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pcDNA3.1-His-Gli1

Expression vector of mouse Gli1 cDNA

Catalog number RDB08064
Resource name pcDNA3.1-His-Gli1
Clone info. Expression vector of mouse Gli1 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Gli1 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Gli1 (NCBI Gene 14632) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08064 pcDNA3.1-His-Gli1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0074j (Japanese text)
Featured content Dnaconda's recommendation EXR0074e (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB17503_A9Ibp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: CMV pro, 6xHis, T7 tag, Xpress tag, insert 5' | Primer: CMV_Forward | Sequence: RDB17503_A9Iba.seq
>08064_17503_A9Ib_CMV_Forward_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNGN NGCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACC ATGGGGGGTT
  181 CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGG
  241 ATCTGTACGA CGATGACGAT AAGGTACCTA AGGATCCAGT GTGGTGGAAT TCTGCAGATG
  301 ACGCCATGTT CAATCCAATG ACTCCACCAC AAGTCAATAG CTATAGTGAG CCATGCTGTC
  361 TCCGACCCCT CCACAGCCAA GGAGTCCCCA GCATGGGAAC AGAAGGACTT TCTGGTCTGC
  421 CCTTTTGCCA CCAAGCCAAC TTTATGTCAG GGTCCCAGGG TTATGGAGCA GCCAGAGAGA
  481 CCAGCAGCTG CACTGAAGGA TCTCTCTTTC CTCCTCCTCC TCCTCCTCGG AGTTCAGTCA
  541 AATTAACAAA GAAGCGGGCT CTCTCCATCT CGCCCCTTTC TGATGCCAGC CTCGACCTGC
  601 AAACCGTAAT CCGGACCTCA CCCAGCTCCC TGGTGGCTTT CATCAACTCT CGCTGTACAT
  661 CTCCGGGCGG TTCCTACGGC CATCTCTCCA TTGGTACCAT GAGCCCTTCT TTAGGATTCC
  721 CACCTCAGAT GAGTCATCAA AAAGGAACTT CACCTCCCTA TGGAGTCCAG CCCTGTGTTC
  781 CACATGACTC TACTCGGGGT TCAATGATGC TTCACCCCCA GGCCNGGGGA NCACGTGCAA
  841 CCTGCCAGCT GAANTCAGAN CTGGATATGA NGGTTGGCAA GTGCCCGGNN GNNCCTTTGG
  901 AANGGGANAT GNNTAGCCCC NACTCCNCAN GCNTACNNGN NCNNNNNNNG GGGNNGCTGG
961 NTNGGCGGNN NGNNCTNNNN ANANANGANA ANC
//
Region: BGH p(A), insert 3' | Primer: BGH_rev2 | Sequence: RDB17503_A9Ibb.seq
>08064_17503_A9Ib_BGH_rev2_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG GGNNGGNNNN NNACAGANGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGACTC GAGCGGCCGC TTAGGCACTA
  121 GAGTTGAGGA ATTGTGTCTC TCCAGGCAGA GACCCCAGCA AGACACTCAT GTTACCCACT
  181 GCCATGTTGG GGGGCCCAGA GGGAGATGGG GTGTTTTTAG AGCTGTCCCC TTGCTCATGG
  241 GAAAGAGGAG GGCTCAGGCC CTGGGCCTCA TCTAGGATAG CCACAAAGTC CAGCTGAGTG
  301 TTGTCCAGGT CAAGAGAGTC CAGAGCGTTA CACACCTGCC CTTCAGGAGG AGGGTACAAG
  361 GCAGGGCCTG CTCCTAACCT GCCCACCTCA GGATGGCCAC AGCTGGGGTT GGTATCCCCC
  421 ACCTTGAGGG GCCCATAGCA AGGGGACAGC GGGGGCAGAA GTCGGGGTGG TGCTGCTGGC
  481 CTGTGGGAAG GCCTGTTTAC TCCCACGGTG AAAGTTTCAT GGCAGGGTGA AGGGGCAGGA
  541 TAGGAGCCTG ATTTGTGATT GGCCGAAGCA GGTGCAAAGC CAGATCCATA TGCTGCCGCC
  601 GCTGCTGCTG GGGTCTTGGC AGGGCTCTGA CTAACTTGGG AACCCCCCAG AAACTTGGGG
  661 CTCTGGTATG GGAGTTCCTG GTTGGGGAGC CCTCCCCGGT TTCTTCCCTC CCACAACAAT
  721 TCCTGCTGCG ACTGAACGTA ATTACACACC AGCTGANCTT TGAGCTGTCC TGTGGAATGA
  781 GAGGANGANG GGTTGAAATT GANGNCAAGC CTGNNTTCTG TTGAGAGATA ACCATGGNGA
  841 GGCTGAGGAT AGGGANNNGA CTGGGGGNTA CTGGGGCTGA NGGANCTGGG GANGATGTGA
  901 AAACAGANGC TGCNGGCCTG GGGNNNCCNN NACNGGGGGN GGGCATTGAN GNNNNNGNNN
961 ATCCNGNGGN NNCNNANCNN NCNNGNCACC NNNNNTNNNN GNNTTTNNC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
user report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).

2019.10.11

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