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pcDNA3.1-His-Gli1

Expression vector of mouse Gli1 cDNA

Catalog number RDB08064
Resource name pcDNA3.1-His-Gli1
Clone info. Expression vector of mouse Gli1 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Gli1 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Gli1 (NCBI Gene 14632) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08064 pcDNA3.1-His-Gli1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08064_A3Fo.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08064_A3Foa.seq
>08064_08064_A3Fo_T7_20bp_H05_23.ab1
    1 CAAGCTGGCT AGCGTTTAAC TTAAGCTTAC CATGGGGGGT TCTCATCATC ATCATCATCA
   61 TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA
  121 TAAGGTACCT AAGGATCCAG TGTGGTGGAA TTCTGCAGAT GACGCCATGT TCAATCCAAT
  181 GACTCCACCA CAAGTCAATA GCTATAGTGA GCCATGCTGT CTCCGACCCC TCCACAGCCA
  241 AGGAGTCCCC AGCATGGGAA CAGAAGGACT TTCTGGTCTG CCCTTTTGCC ACCAAGCCAA
  301 CTTTATGTCA GGGTCCCAGG GTTATGGAGC AGCCAGAGAG ACCAGCAGCT GCACTGAAGG
  361 ATCTCTCTTT CCTCCTCCTC CTCCTCCTCG GAGTTCAGTC AAATTAACAA AGAAGCGGGC
  421 TCTCTCCATC TCGCCCCTTT CTGATGCCAG CCTCGACCTG CAAACCGTAA TCCGGACCTC
  481 ACCCAGCTCC CTGGTGGCTT TCATCAACTC TCGCTGTACA TCTCCGGGCG GTTCCTACGG
  541 CCATCTCTCC ATTGGTACCA TGAGCCCTTC TTTAGGATTC CCACCTCAGA TGAGTCATCA
  601 AAAAGGAACT TCACCTCCCT ATGGAGTCCA GCCCTGTGTT CCACATGACT CTACTCGGGG
  661 TTCAATGATG CTTCACCCCC AGGCCCGGGG ACCACGTGCA ACCTGCCAGC TGAAGTCAGA
  721 GCTGGATATG ATGGTTGGCA AGTGCCCGGA GGACCCTTTG GAAGGGGACA TGTCTAGCCC
  781 CAACTCCACA GGCATACAGG ATCACCTGTT GGGGATGCTG GATGGGCGGG AGGACCTGGA
  841 GAGAGAGGAG AAGCCTGAGC CTGAGTCTGT GTATGAGACA GACTGCCGCT GGGATGGTTG
  901 CAGCCAGGAG TTCGATTCCC AGGAGCAGCT GGGTGCACCA CATCAACAGT GAGCATATCC
  961 ACGGGGAGCG GAACGAATTT CGTGTGCCAT TGGGGGAGTG CTCCAGGAGC TGAGCCCTTT
 1021 CAGCCATACA TGCTGCTGCT GCACATGCGC AGACCCACGG CGAGAGCTAA CGAGTGCACG
1081 ATGAGGCCTG CTCCGTAAGT TCCTAAATTT CAACG
//
Primer: BGH_rev RDB08064_A3Fob.seq
>08064_08064_A3Fo_BGHrev_G04_19.ab1
    1 AAAACGGAAC TCTAGACTCG AGCGGCCGCT TAGGCACTAG AGTTGAGGAA TTGTGTCTCT
   61 CCAGGCAGAG ACCCCAGCAA GACACTCATG TTACCCACTG CCATGTTGGG GGGCCCAGAG
  121 GGAGATGGGG TGTTTTTAGA GCTGTCCCCT TGCTCATGGG AAAGAGGAGG GCTCAGGCCC
  181 TGGGCCTCAT CTAGGATAGC CACAAAGTCC AGCTGAGTGT TGTCCAGGTC AAGAGAGTCC
  241 AGAGCGTTAC ACACCTGCCC TTCAGGAGGA GGGTACAAGG CAGGGCCTGC TCCTAACCTG
  301 CCCACCTCAG GATGGCCACA GCTGGGGTTG GTATCCCCCA CCTTGAGGGG CCCATAGCAA
  361 GGGGACAGCG GGGGCAGAAG TCGGGGTGGT GCTGCTGGCC TGTGGGAAGG CCTGTTTACT
  421 CCCACGGTGA AAGTTTCATG GCAGGGTGAA GGGGCAGGAT AGGAGCCTGA TTTGTGATTG
  481 GCCGAAGCAG GTGCAAAGCC AGATCCATAT GCTGCCGCCG CTGCTGCTGG GGTCTTGGCA
  541 GGGCTCTGAC TAACTTGGGA ACCCCCCAGA AACTTGGGGC TCTGGTATGG GAGTTCCTGG
  601 TTGGGGAGCC CTCCCCGGTT TCTTCCCTCC CACAACAATT CCTGCTGCGA CTGAACGTAA
  661 TTACACACCA GCTGAGCTTT GAGCTGTCCT GTGGAATGAG AGGAGGAGGG GTTGAAATTG
  721 AGGCCAAGCC TGGGTTCTGT TGAGAGATAA CCATGGGGAG GCTGAGGATA GGGACCCGAC
  781 TGGGGATACT GGGGCTGAGG GAGCTGGGGA TGATGTGAAA ACAGAGGCTG CGGGCCTGGG
  841 GGACCCTTCA CTGGGGTGGG CATTGAGGCA GGGTGCCAAT CCGGTGGAGT CAGACCCCAC
  901 TGGGCACCCT TGTTCTGGTT TTACCTGCAC TTGTCCATAA TGCTCAAGTC GAGGACACTG
  961 GCTATAGGCA GCACCCGGGA GCCCTTATTG CTAGGGTACA CCCCCAGCAT GAGAAGGGGA
 1021 ACTCACCCCC AAGTTTTCTG GGGTGGGAAT CAGGATAGGA GACTTGCCTG GGCACAGTTG
1081 ATCAGGTCAT AGTTGATTGG TGCACCAGGA CCCAGGAAGG CAGGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
user report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).

2018.09.03

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