Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-His-Gli1

Expression vector of mouse Gli1 cDNA

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17503_A9Ibp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB17503_A9Ibd.seq
>08064_17503_A9Ib_3_CMV_Forward_A02_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNNGCANTG GGCGGTAGGC GTGTACGGNG GGANGTCTAT    61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT   121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT ACCATGGGGG   181 GTTCTCATCA TCATCATCAT CATGGTATGG CTAGCATGAC TGGTGGACAG CAAATGGGTC   241 GGGATCTGTA CGACGATGAC GATAAGGTAC CTAAGGATCC AGTGTGGTGG AATTCTGCAG   301 ATGACGCCAT GTTCAATCCA ATGACTCCAC CACAAGTCAA TAGCTATAGT GAGCCATGCT   361 GTCTCCGACC CCTCCACAGC CAAGGAGTCC CCAGCATGGG AACAGAAGGA CTTTCTGGTC   421 TGCCCTTTTG CCACCAAGCC AACTTTATGT CAGGGTCCCA GGGTTATGGA GCAGCCAGAG   481 AGACCAGCAG CTGCACTGAA GGATCTCTCT TTCCTCCTCC TCCTCCTCCT CGGAGTTCAG   541 TCAAATTAAC AAAGAAGCGG GCTCTCTCCA TCTCGCCCCT TTCTGATGCC AGCCTCGACC   601 TGCAAACCGT AATCCGGACC TCACCCAGCT CCCTGGTGGC TTTCATCAAC TCTCGCTGTA   661 CATCTCCGGG CGGTTCCTAC GGCCATCTCT CCATTGGTAC CATGAGCCCT TCTTTAGGAT   721 TCCCACCTCA GATGAGTCAT CAAAAAGGAA CTTCACCTCC CTATGGAGTC CAGCCCTGTG   781 TTCCACATGA CTCTACTCGG GGTTCAATGA TGCTTCACCC CNAGGCCCGG GGANCACGTG   841 CAACCTGCCA GCTGAAGTCA GANCTGGATA TGATGNTTGG NAAGTGCCCG GANGNNCCNT   901 TNGANNGGGA NATGNCTAGC CCCAACNNNN CANGNNTACA NGATCACCTG TNGGGGANGC 961 TGGNNGGNNN GNANGNNCNN NNNNNANANG ANAANNCCNN ANCNNNAN //
Primer: bGH-B_R (Pr0746) Region: insert 3' Sequence file: RDB17503_A9Ibe.seq
>08064_17503_A9Ib_3_bGH-B_R_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGTNNNNN NGGCCCTCTA GANTCGAGCG    61 GCCGCTTAGG CACTAGAGTT GAGGAATTGT GTCTCTCCAG GCAGAGACCC CAGCAAGACA   121 CTCATGTTAC CCACTGCCAT GTTGGGGGGC CCAGAGGGAG ATGGGGTGTT TTTAGAGCTG   181 TCCCCTTGCT CATGGGAAAG AGGAGGGCTC AGGCCCTGGG CCTCATCTAG GATAGCCACA   241 AAGTCCAGCT GAGTGTTGTC CAGGTCAAGA GAGTCCAGAG CGTTACACAC CTGCCCTTCA   301 GGAGGAGGGT ACAAGGCAGG GCCTGCTCCT AACCTGCCCA CCTCAGGATG GCCACAGCTG   361 GGGTTGGTAT CCCCCACCTT GAGGGGCCCA TAGCAAGGGG ACAGCGGGGG CAGAAGTCGG   421 GGTGGTGCTG CTGGCCTGTG GGAAGGCCTG TTTACTCCCA CGGTGAAAGT TTCATGGCAG   481 GGTGAAGGGG CAGGATAGGA GCCTGATTTG TGATTGGCCG AAGCAGGTGC AAAGCCAGAT   541 CCATATGCTG CCGCCGCTGC TGCTGGGGTC TTGGCAGGGC TCTGACTAAC TTGGGAACCC   601 CCCANAAACT TGGGGCTCTG GTATGGGAGT TCCTGGTTGG GGAGCCCTCC CCGGTTTCTT   661 CCCTCCCACA ACAATTCCTG CTGCGACTGA ACGTAATTAC ACACCAGCTG AGCTTTGAGC   721 TGTCCTGTGG AATGAGAGGA GGAGGGGTTG AAATTGANGC CAAGCCTGGG TTCTGTTGAG   781 AGATAACCAT GGGGANGCTG ANGATAGGGA NCCGACTGGG GNTACTGNNG CTGANGGANC   841 TGGGGATGAT GTGAAAACAG ANGCTGCNGG NCCTGNNNAN CCTTCNCTGG GGNGGGCATT 901 NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, NeoR Sequence file: RDB17503_A9Ibf.seq
>08064_17503_A9Ib_3_SV40pro_ori_F_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCCTCTG CCTCTGANCN NTTCNNGAAG TAGTGAGGAG    61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG   121 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG   181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA   241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG   301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT   361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA   421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC   481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG   541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG   601 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG   661 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA NGATCTCGTC GTGACCCATG   721 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT   781 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG   841 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCNT GCTTTACGGN ATCNNNGCTC   901 CNGATTCGCA GNGCATCNCC TTCTATCNNN TTCTNGACNA NTNCTTCTGA NCGGGNNTCN   961 GGGGNNTCGA AANTGANCGA NCNANNNNNN NCCNANCNTG NCNNNCNNNN NNNNTNNNAN 1021 TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl