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pcDNA3.1-His-Gli1

Expression vector of mouse Gli1 cDNA

Catalog number RDB08064
Resource name pcDNA3.1-His-Gli1
Clone info. Expression vector of mouse Gli1 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Gli1 cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Mouse Gli1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08064 pcDNA3.1-His-Gli1 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0074e (English text)
          Featured content Dnaconda's recommendation EXR0074j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17503_A9Ibp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, 6xHis, T7 tag, Xpress tag, insert 5'
          Sequence file: RDB17503_A9Ibd.seq check
          >08064_17503_A9Ib_3_CMV_Forward_A02_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNT NNNNGCANTG GGCGGTAGGC GTGTACGGNG GGANGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT ACCATGGGGG
            181 GTTCTCATCA TCATCATCAT CATGGTATGG CTAGCATGAC TGGTGGACAG CAAATGGGTC
            241 GGGATCTGTA CGACGATGAC GATAAGGTAC CTAAGGATCC AGTGTGGTGG AATTCTGCAG
            301 ATGACGCCAT GTTCAATCCA ATGACTCCAC CACAAGTCAA TAGCTATAGT GAGCCATGCT
            361 GTCTCCGACC CCTCCACAGC CAAGGAGTCC CCAGCATGGG AACAGAAGGA CTTTCTGGTC
            421 TGCCCTTTTG CCACCAAGCC AACTTTATGT CAGGGTCCCA GGGTTATGGA GCAGCCAGAG
            481 AGACCAGCAG CTGCACTGAA GGATCTCTCT TTCCTCCTCC TCCTCCTCCT CGGAGTTCAG
            541 TCAAATTAAC AAAGAAGCGG GCTCTCTCCA TCTCGCCCCT TTCTGATGCC AGCCTCGACC
            601 TGCAAACCGT AATCCGGACC TCACCCAGCT CCCTGGTGGC TTTCATCAAC TCTCGCTGTA
            661 CATCTCCGGG CGGTTCCTAC GGCCATCTCT CCATTGGTAC CATGAGCCCT TCTTTAGGAT
            721 TCCCACCTCA GATGAGTCAT CAAAAAGGAA CTTCACCTCC CTATGGAGTC CAGCCCTGTG
            781 TTCCACATGA CTCTACTCGG GGTTCAATGA TGCTTCACCC CNAGGCCCGG GGANCACGTG
            841 CAACCTGCCA GCTGAAGTCA GANCTGGATA TGATGNTTGG NAAGTGCCCG GANGNNCCNT
            901 TNGANNGGGA NATGNCTAGC CCCAACNNNN CANGNNTACA NGATCACCTG TNGGGGANGC
          961 TGGNNGGNNN GNANGNNCNN NNNNNANANG ANAANNCCNN ANCNNNAN
          //
          Primer: bGH-B_R
          Region: insert 3'
          Sequence file: RDB17503_A9Ibe.seq check
          >08064_17503_A9Ib_3_bGH-B_R_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGTNNNNN NGGCCCTCTA GANTCGAGCG
             61 GCCGCTTAGG CACTAGAGTT GAGGAATTGT GTCTCTCCAG GCAGAGACCC CAGCAAGACA
            121 CTCATGTTAC CCACTGCCAT GTTGGGGGGC CCAGAGGGAG ATGGGGTGTT TTTAGAGCTG
            181 TCCCCTTGCT CATGGGAAAG AGGAGGGCTC AGGCCCTGGG CCTCATCTAG GATAGCCACA
            241 AAGTCCAGCT GAGTGTTGTC CAGGTCAAGA GAGTCCAGAG CGTTACACAC CTGCCCTTCA
            301 GGAGGAGGGT ACAAGGCAGG GCCTGCTCCT AACCTGCCCA CCTCAGGATG GCCACAGCTG
            361 GGGTTGGTAT CCCCCACCTT GAGGGGCCCA TAGCAAGGGG ACAGCGGGGG CAGAAGTCGG
            421 GGTGGTGCTG CTGGCCTGTG GGAAGGCCTG TTTACTCCCA CGGTGAAAGT TTCATGGCAG
            481 GGTGAAGGGG CAGGATAGGA GCCTGATTTG TGATTGGCCG AAGCAGGTGC AAAGCCAGAT
            541 CCATATGCTG CCGCCGCTGC TGCTGGGGTC TTGGCAGGGC TCTGACTAAC TTGGGAACCC
            601 CCCANAAACT TGGGGCTCTG GTATGGGAGT TCCTGGTTGG GGAGCCCTCC CCGGTTTCTT
            661 CCCTCCCACA ACAATTCCTG CTGCGACTGA ACGTAATTAC ACACCAGCTG AGCTTTGAGC
            721 TGTCCTGTGG AATGAGAGGA GGAGGGGTTG AAATTGANGC CAAGCCTGGG TTCTGTTGAG
            781 AGATAACCAT GGGGANGCTG ANGATAGGGA NCCGACTGGG GNTACTGNNG CTGANGGANC
            841 TGGGGATGAT GTGAAAACAG ANGCTGCNGG NCCTGNNNAN CCTTCNCTGG GGNGGGCATT
          901 NNNNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori, NeoR
          Sequence file: RDB17503_A9Ibf.seq check
          >08064_17503_A9Ib_3_SV40pro_ori_F_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNCCTCTG CCTCTGANCN NTTCNNGAAG TAGTGAGGAG
             61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
            121 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
            181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
            241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
            301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
            361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
            421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
            481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
            541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
            601 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
            661 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA NGATCTCGTC GTGACCCATG
            721 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
            781 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
            841 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCNT GCTTTACGGN ATCNNNGCTC
            901 CNGATTCGCA GNGCATCNCC TTCTATCNNN TTCTNGACNA NTNCTTCTGA NCGGGNNTCN
            961 GGGGNNTCGA AANTGANCGA NCNANNNNNN NCCNANCNTG NCNNNCNNNN NNNNTNNNAN
          1021 TNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
          user report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl