Please review the QC test results indicated by icon below as well as clone information before placing your order.

8xm3'Gli-BS-delta51-LucII

Reporter plasmid carring eight copies of mutated Gli-binding site

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08062_A6Cep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3-RV6R Sequence file: RDB08062_A6Cea.seq
>08062_08062_A6Ce_pGL3_RV6R_E09_15.ab1     1 GTCTCCATCA AAAGGTGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATTT    61 TACCAACAGT ACCGGAATGC CAAGCTTGGC TGCCCGCACA GCCCTGCTCA CCGTCTCCGC   121 AGCTCAGCCC TGGCTGGTCG CTCCGTGCTC TCCGGTCTCA CAGCCCCGCT TTTACGTGCC   181 CCGGAGCCTG GGCTGGCCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGAAC AGATTCGCGA   241 TCGAGAGCTT CTACTTCCCA CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG   301 CTTGTCGAGA GCTTCTACTT CCCACTTCCC TGCTTGTCGA GAGCTTCTAC TTCCCACTTC   361 CCTGCTTGTC GAGAGCTTCT ACTTCCCACT TCCCTGCTTG TCGAGAGCTT CTACTTCCCA   421 CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG CTTGTCGAGA GCTTCTACTT   481 CCCACTTCCC TGCTTGTCGA TCGCGAATCT GTTCCCGGGG ATCCAGACAT GATAAGATAC   541 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA   601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC   661 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG   721 TAAAACCTCT ACAAATGTGA TATGGCTGAT CCAGACATGA TAAGATACAT TGATGAGTTT   781 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT   841 ATTGCTTTAT TTTGTAACCA TTATAAAGCT GCAATAAACA AGTTAACAAC AATTTGCATT   901 TCATTTTATG TTTCAGGTTC AGGGGGGAGG TGTGGGGAGG GTTTTTTAAA GCAAGTAAAA   961 CCTCCTACAA TGTGATATGG CTGAATCCCT GGCCTTCGCG GCGGTTTCGG TGGATGGACG  1021 GGTTGAAAAA ACTCTGGAAC ACATGCAAGC TCCCAGGAAA GACGGTCACA GCCTTGTCTG  1081 GTAAGCCGGA TGGCCGGGAA GCAGAACCAA GCCTCGGTCC AGGGTCGGCC GGTTCAAGCC 1141 GAG //
Primer: Luc-C_F Sequence file: RDB08062_A6Ceb.seq
>08062_08062_A6Ce_Luc-C_F_D12_04_ABI08.ab1     1 TAAAACGGAT CGCCAGTCAA GTAACAACCG CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG    61 TGGACGAAGT ACCGAAAGGT CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC   121 TCATAAAGGC CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG   181 ACGAAATTCT TAGCTATTGT AATACTCTAG AGGATCTTTG TGAAGGAACC TTACTTCTGT   241 GGTGTGACAT AATTGGACAA ACTACCTACA GAGATTTAAA GCTCTAAGGT AAATATAAAA   301 TTTTTAAGTG TATAATGTGT TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA   361 ACCTATGGAA CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT   421 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA ACATTCTACT   481 CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT TTCCTTCAGA ATTGCTAAGT   541 TTTTTGAGTC ATGCTGTGTT TAGTAATAGA ACTCTTGCTT GCTTTGCTAT TTACACCACA   601 AAGGAAAAAG CTGCACTGCT ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA   661 AGTAGGCATA ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA   721 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT AATTTGTAAA   781 GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA GAGATCATAA TCAGCCATAC   841 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA   901 ACATAAAATG AAATGCAATT TGTTGTTGTT AACTTTGTTT ATTGCAGCTT ATAATGGTTT   961 ACAAATAAAG CAATAGCATC ACAAATTTCA ACAAATAAAG CATTTTTTTT CACTGCATTC  1021 TAGTTGTGGG TTGTCCCAAA CTCATCAATG TATCTTATCA TGGTCTGCCT CCGAAGGCCA  1081 AGAAAGCTTC CTCTGTGTTC CTCATAAAAC CCTAACCTTC CTCTACTTGA GAGGACAATT  1141 CCAATCATAG GGCCTGCCAA TCCACCCTCA GTGGTCTTCT TGTATTAAGG TCACTACCAA 1201 AAGGAAATTG GGGTAGGGGG GTTCTTTTTC CCATGCGGA //
Primer: RDB08068_F1 Sequence file: RDB08062_A6Cec.seq
>08062_08062_A6Ce_RDB8068_F1_E12_05_ABI08.ab1     1 CGGTCAGGAT GGGAGGGGAC TTCCCAGATA TTTTAAAATT ACCCTTAGAA AGCGGTCTGT    61 GAAAAACCCC TACCCAATTT CCTTTTTGTT AAGTGACCTA ATTAACAGGA GGACACAGAG   121 GGTGGATGGG CAGCCTATGA TTGGAATGTC CTCTCAAGTA GAGGAGGTTA GGGTTTATGA   181 GGACACAGAG GAGCTTCCTG GGCCTCGAGG CAGACATGAT AAGATACATT GATGAGTTTG   241 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA   301 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC 361 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2020.12.30

GNP_filter3_RDBDEP_html_201222.pl