Sequencing and PCR primers for primer information.
Reporter plasmid carring eight copies of mutated Gli-binding site
| Catalog number | RDB08062 |
|---|---|
| Resource name | 8xm3'Gli-BS-delta51-LucII |
| Clone info. | Reporter plasmid carring eight copies of mutated Gli-binding site. Use as a negative control of 8x3'Gli-BS-delta51-LucII (cat.#08061). |
| Vector backbone | delta51-LucII (Plasmid) |
| Size of vector backbone | 6.1 kb |
| Selectable markers | Amp^r |
| Gene/insert name | firefly (Photinus pyralis) luciferase cDNA |
| Depositor | Sasaki, Hiroshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Development, 124, 1313-1322 (1997).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB08062 | 8xm3'Gli-BS-delta51-LucII | DNA solution |
Electronic file
Featured content
| Featured content | Dnaconda's recommendation EXR0001j (Japanese text) |
|---|---|
| Featured content | Promoter Collection (Outline) (English text) |
| Featured content | Promoter Collection (Outline) (Japanese text) |
| Featured content | Hedgehog signaling (English text) |
References
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB08062_A6Cep1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pGL3-RV6R | RDB08062_A6Cea.seq |
|---|---|
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>08062_08062_A6Ce_pGL3_RV6R_E09_15.ab1 1 GTCTCCATCA AAAGGTGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATTT 61 TACCAACAGT ACCGGAATGC CAAGCTTGGC TGCCCGCACA GCCCTGCTCA CCGTCTCCGC 121 AGCTCAGCCC TGGCTGGTCG CTCCGTGCTC TCCGGTCTCA CAGCCCCGCT TTTACGTGCC 181 CCGGAGCCTG GGCTGGCCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGAAC AGATTCGCGA 241 TCGAGAGCTT CTACTTCCCA CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG 301 CTTGTCGAGA GCTTCTACTT CCCACTTCCC TGCTTGTCGA GAGCTTCTAC TTCCCACTTC 361 CCTGCTTGTC GAGAGCTTCT ACTTCCCACT TCCCTGCTTG TCGAGAGCTT CTACTTCCCA 421 CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG CTTGTCGAGA GCTTCTACTT 481 CCCACTTCCC TGCTTGTCGA TCGCGAATCT GTTCCCGGGG ATCCAGACAT GATAAGATAC 541 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA 601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC 661 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG 721 TAAAACCTCT ACAAATGTGA TATGGCTGAT CCAGACATGA TAAGATACAT TGATGAGTTT 781 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT 841 ATTGCTTTAT TTTGTAACCA TTATAAAGCT GCAATAAACA AGTTAACAAC AATTTGCATT 901 TCATTTTATG TTTCAGGTTC AGGGGGGAGG TGTGGGGAGG GTTTTTTAAA GCAAGTAAAA 961 CCTCCTACAA TGTGATATGG CTGAATCCCT GGCCTTCGCG GCGGTTTCGG TGGATGGACG 1021 GGTTGAAAAA ACTCTGGAAC ACATGCAAGC TCCCAGGAAA GACGGTCACA GCCTTGTCTG 1081 GTAAGCCGGA TGGCCGGGAA GCAGAACCAA GCCTCGGTCC AGGGTCGGCC GGTTCAAGCC 1141 GAG // |
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| Primer: Luc-C_F | RDB08062_A6Ceb.seq |
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>08062_08062_A6Ce_Luc-C_F_D12_04_ABI08.ab1 1 TAAAACGGAT CGCCAGTCAA GTAACAACCG CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG 61 TGGACGAAGT ACCGAAAGGT CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC 121 TCATAAAGGC CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG 181 ACGAAATTCT TAGCTATTGT AATACTCTAG AGGATCTTTG TGAAGGAACC TTACTTCTGT 241 GGTGTGACAT AATTGGACAA ACTACCTACA GAGATTTAAA GCTCTAAGGT AAATATAAAA 301 TTTTTAAGTG TATAATGTGT TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA 361 ACCTATGGAA CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT 421 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA ACATTCTACT 481 CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT TTCCTTCAGA ATTGCTAAGT 541 TTTTTGAGTC ATGCTGTGTT TAGTAATAGA ACTCTTGCTT GCTTTGCTAT TTACACCACA 601 AAGGAAAAAG CTGCACTGCT ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA 661 AGTAGGCATA ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA 721 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT AATTTGTAAA 781 GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA GAGATCATAA TCAGCCATAC 841 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA 901 ACATAAAATG AAATGCAATT TGTTGTTGTT AACTTTGTTT ATTGCAGCTT ATAATGGTTT 961 ACAAATAAAG CAATAGCATC ACAAATTTCA ACAAATAAAG CATTTTTTTT CACTGCATTC 1021 TAGTTGTGGG TTGTCCCAAA CTCATCAATG TATCTTATCA TGGTCTGCCT CCGAAGGCCA 1081 AGAAAGCTTC CTCTGTGTTC CTCATAAAAC CCTAACCTTC CTCTACTTGA GAGGACAATT 1141 CCAATCATAG GGCCTGCCAA TCCACCCTCA GTGGTCTTCT TGTATTAAGG TCACTACCAA 1201 AAGGAAATTG GGGTAGGGGG GTTCTTTTTC CCATGCGGA // |
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| Primer: RDB08068_F1 | RDB08062_A6Cec.seq |
|
>08062_08062_A6Ce_RDB8068_F1_E12_05_ABI08.ab1 1 CGGTCAGGAT GGGAGGGGAC TTCCCAGATA TTTTAAAATT ACCCTTAGAA AGCGGTCTGT 61 GAAAAACCCC TACCCAATTT CCTTTTTGTT AAGTGACCTA ATTAACAGGA GGACACAGAG 121 GGTGGATGGG CAGCCTATGA TTGGAATGTC CTCTCAAGTA GAGGAGGTTA GGGTTTATGA 181 GGACACAGAG GAGCTTCCTG GGCCTCGAGG CAGACATGAT AAGATACATT GATGAGTTTG 241 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA 301 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC 361 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTT // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802. |
|---|---|
| reference | Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133. |
| user report | Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014). |
| user report | Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987. |
2019.03.07
GNP_filter3_RDBDEP_html_190307.pl