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8xm3'Gli-BS-delta51-LucII

Reporter plasmid carring eight copies of mutated Gli-binding site

Catalog number RDB08062
Resource name 8xm3'Gli-BS-delta51-LucII
Clone info. Reporter plasmid carring eight copies of mutated Gli-binding site.
Use as a negative control of 8x3'Gli-BS-delta51-LucII (cat.#08061).
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Sasaki, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Development, 124, 1313-1322 (1997).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08062 8xm3'Gli-BS-delta51-LucII DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0001j (Japanese text)
Featured content Promoter Collection (Outline) (English text)
Featured content Promoter Collection (Outline) (Japanese text)
Featured content Hedgehog signaling (English text)

References

original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08062_A6Cep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3-RV6R RDB08062_A6Cea.seq
>08062_08062_A6Ce_pGL3_RV6R_E09_15.ab1
    1 GTCTCCATCA AAAGGTGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATTT
   61 TACCAACAGT ACCGGAATGC CAAGCTTGGC TGCCCGCACA GCCCTGCTCA CCGTCTCCGC
  121 AGCTCAGCCC TGGCTGGTCG CTCCGTGCTC TCCGGTCTCA CAGCCCCGCT TTTACGTGCC
  181 CCGGAGCCTG GGCTGGCCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGAAC AGATTCGCGA
  241 TCGAGAGCTT CTACTTCCCA CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG
  301 CTTGTCGAGA GCTTCTACTT CCCACTTCCC TGCTTGTCGA GAGCTTCTAC TTCCCACTTC
  361 CCTGCTTGTC GAGAGCTTCT ACTTCCCACT TCCCTGCTTG TCGAGAGCTT CTACTTCCCA
  421 CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG CTTGTCGAGA GCTTCTACTT
  481 CCCACTTCCC TGCTTGTCGA TCGCGAATCT GTTCCCGGGG ATCCAGACAT GATAAGATAC
  541 ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA
  601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC
  661 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
  721 TAAAACCTCT ACAAATGTGA TATGGCTGAT CCAGACATGA TAAGATACAT TGATGAGTTT
  781 GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT
  841 ATTGCTTTAT TTTGTAACCA TTATAAAGCT GCAATAAACA AGTTAACAAC AATTTGCATT
  901 TCATTTTATG TTTCAGGTTC AGGGGGGAGG TGTGGGGAGG GTTTTTTAAA GCAAGTAAAA
  961 CCTCCTACAA TGTGATATGG CTGAATCCCT GGCCTTCGCG GCGGTTTCGG TGGATGGACG
 1021 GGTTGAAAAA ACTCTGGAAC ACATGCAAGC TCCCAGGAAA GACGGTCACA GCCTTGTCTG
 1081 GTAAGCCGGA TGGCCGGGAA GCAGAACCAA GCCTCGGTCC AGGGTCGGCC GGTTCAAGCC
1141 GAG
//
Primer: Luc-C_F RDB08062_A6Ceb.seq
>08062_08062_A6Ce_Luc-C_F_D12_04_ABI08.ab1
    1 TAAAACGGAT CGCCAGTCAA GTAACAACCG CGAAAAAGTT GCGCGGAGGA GTTGTGTTTG
   61 TGGACGAAGT ACCGAAAGGT CTTACCGGAA AACTCGACGC AAGAAAAATC AGAGAGATCC
  121 TCATAAAGGC CAAGAAGGGC GGAAAGTCCA AATTGTAAAA TGTAACTGTA TTCAGCGATG
  181 ACGAAATTCT TAGCTATTGT AATACTCTAG AGGATCTTTG TGAAGGAACC TTACTTCTGT
  241 GGTGTGACAT AATTGGACAA ACTACCTACA GAGATTTAAA GCTCTAAGGT AAATATAAAA
  301 TTTTTAAGTG TATAATGTGT TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA
  361 ACCTATGGAA CTGATGAATG GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT
  421 TGCTCAGAAG AAATGCCATC TAGTGATGAT GAGGCTACTG CTGACTCTCA ACATTCTACT
  481 CCTCCAAAAA AGAAGAGAAA GGTAGAAGAC CCCAAGGACT TTCCTTCAGA ATTGCTAAGT
  541 TTTTTGAGTC ATGCTGTGTT TAGTAATAGA ACTCTTGCTT GCTTTGCTAT TTACACCACA
  601 AAGGAAAAAG CTGCACTGCT ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA
  661 AGTAGGCATA ACAGTTATAA TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA
  721 GTGTCTGCTA TTAATAACTA TGCTCAAAAA TTGTGTACCT TTAGCTTTTT AATTTGTAAA
  781 GGGGTTAATA AGGAATATTT GATGTATAGT GCCTTGACTA GAGATCATAA TCAGCCATAC
  841 CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA
  901 ACATAAAATG AAATGCAATT TGTTGTTGTT AACTTTGTTT ATTGCAGCTT ATAATGGTTT
  961 ACAAATAAAG CAATAGCATC ACAAATTTCA ACAAATAAAG CATTTTTTTT CACTGCATTC
 1021 TAGTTGTGGG TTGTCCCAAA CTCATCAATG TATCTTATCA TGGTCTGCCT CCGAAGGCCA
 1081 AGAAAGCTTC CTCTGTGTTC CTCATAAAAC CCTAACCTTC CTCTACTTGA GAGGACAATT
 1141 CCAATCATAG GGCCTGCCAA TCCACCCTCA GTGGTCTTCT TGTATTAAGG TCACTACCAA
1201 AAGGAAATTG GGGTAGGGGG GTTCTTTTTC CCATGCGGA
//
Primer: RDB08068_F1 RDB08062_A6Cec.seq
>08062_08062_A6Ce_RDB8068_F1_E12_05_ABI08.ab1
    1 CGGTCAGGAT GGGAGGGGAC TTCCCAGATA TTTTAAAATT ACCCTTAGAA AGCGGTCTGT
   61 GAAAAACCCC TACCCAATTT CCTTTTTGTT AAGTGACCTA ATTAACAGGA GGACACAGAG
  121 GGTGGATGGG CAGCCTATGA TTGGAATGTC CTCTCAAGTA GAGGAGGTTA GGGTTTATGA
  181 GGACACAGAG GAGCTTCCTG GGCCTCGAGG CAGACATGAT AAGATACATT GATGAGTTTG
  241 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA
  301 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC
361 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.
reference Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133.
user report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).
user report Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987.

2019.03.07

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