Please review the QC test results indicated by icon below as well as clone information before placing your order.

8xm3'Gli-BS-delta51-LucII

Reporter plasmid carring eight copies of mutated Gli-binding site

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08062_A6Cep1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3_RV6R Region: chicken delta-crystallin Basal Pro,8xm3'Gli-BS,SV40 pA Sequence file: RDB08062_A6Ced.seq
>08062_08062_A6Ce_pGL3_RV6R_E09_15.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNGC CTTTCTTTAT GTTTTTGGCG TCTTCCNTTT    61 TACCAACAGT ACCGGAATGC CAAGCTTGGC TGCCCGCACA GCCCTGCTCA CCGTCTCCGC   121 AGCTCAGCCC TGGCTGGTCG CTCCGTGCTC TCCGGTCTCA CAGCCCCGCT TTTACGTGCC   181 CCGGAGCCTG GGCTGGCCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGAAC AGATTCGCGA   241 TCGAGAGCTT CTACTTCCCA CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG   301 CTTGTCGAGA GCTTCTACTT CCCACTTCCC TGCTTGTCGA GAGCTTCTAC TTCCCACTTC   361 CCTGCTTGTC GAGAGCTTCT ACTTCCCACT TCCCTGCTTG TCGAGAGCTT CTACTTCCCA   421 CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG CTTGTCGAGA GCTTCTACTT   481 CCCACTTCCC TGCTTGTCGA TCGCGAATCT GTTCCCGGGG ATCCAGACAT GATAAGATAC   541 ATTGATGANT TTGGACAAAC CACAACTAGA ATGCANTGAA AAAAATGCTT TATTTGTGAA   601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC   661 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG   721 TAAAACCTCT ACAAATGTGA TATGGCTGAT CCAGACATGA TAAGANACAT TGATGAGTTT   781 GGACAAACCA CAACTAGAAT GCANTGAAAA AAATGCNTTA TTTGTGAAAT TTGTGATGCT   841 ATTGCTTTAT NTTGTAACCN TTANNANNCT GCAATAAACA AGTTANCAAC NANTTGCNTT   901 TCNTTTTATG TTTCNNNNTN NNGGGGGAGG TGTGGGNANG NTTTTTTNAA NNNNNNNAAA 961 CCNNNTNNNN NGTGNNNNGN NTGNNTNCNN NNNNNNNN //
Primer: RDB08068_F1 Region: SV40 pA Sequence file: RDB08062_A6Cee.seq
>08062_08062_A6Ce_RDB8068_F1_E12_05_ABI08.ab1     1 NNNNNNNNNN NNNGNNGGNN TTCCNNGATN NTTTAAAATT ACCCTTAGAA AGCGGTCTGT    61 GAAAAACCCC TACCCAATTT CCTTTTTGTT AAGTGACCTA ATTAACAGGA GGACACAGAG   121 GGTGGATGGG CAGCCTATGA TTGGAATGTC CTCTCAAGTA GAGGAGGTTA GGGTTTATGA   181 GGACACAGAG GAGCTTCCTG GGCCTCGAGG CAGACATGAT AAGATACATT GATGAGTTTG   241 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA   301 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC   361 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAANCNNN NNNANNNNNN 421 NNNN //
Primer: Luc-C_F Region: Luc 3',small t intron,SV40 NLS Sequence file: RDB08062_A6Cef.seq
>08062_08062_A6Ce_Luc-C_F_D12_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNCCNG NCNNGTAACN ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG    61 TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG   121 ATCCTCATAA AGGCCAAGAA GGGCGGAAAG TCCAAATTGT AAAATGTAAC TGTATTCAGC   181 GATGACGAAA TTCTTAGCTA TTGTAATACT CTAGAGGATC TTTGTGAAGG AACCTTACTT   241 CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA AGGTAAATAT   301 AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG TATTTTAGAT   361 TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG AGGAAAACCT   421 GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT CTCAACATTC   481 TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT CAGAATTGCT   541 AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG CTATTTACAC   601 CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT CTGTAACCTT   661 TATAAGTAGG CATAACAGTT ATAATCATAA CATACTGTTT TTTCTTACTC CACACAGGCA   721 TAGAGTGTCT GCTATTAATA ACTATGCTCA AAAATTGTGT ANCTTTAGCT TTTTAATTTG   781 TAAAGGGGTT AATAAGGAAT ATTTGATGTA TAGTGCCTTG NCTAGAGATC NTAATCAGCC   841 ATACNACNTT TGTAGAGGNT TTACTTGCTT TNAAAAANNT CCCACACNTC CCCCTGAACC   901 NGAAACATNA ANNNNATGCN NNTNNNTGNT GTTAANNTNG TTNANTGCAN CTTNNAATGG   961 NTNANAANTA AANNNNNNCA TCNCNNNTTN NNNNAANNAA NNCNNTTTTT TTCNNTGNNN 1021 TCNAGTNNTG GNTNNNNNNA NNNNNTCANN GNNTNNNATN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

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