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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

8xm3'Gli-BS-delta51-LucII

Reporter plasmid carring eight copies of mutated Gli-binding site

Catalog number RDB08062
Resource name 8xm3'Gli-BS-delta51-LucII
Clone info. Reporter plasmid carring eight copies of mutated Gli-binding site.
Comment Use as a negative control of 8x3'Gli-BS-delta51-LucII (cat.#08061).
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
firefly (Photinus pyralis) luciferase

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Development, 124, 1313-1322 (1997)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 124, 1313-1322 (1997).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08062 8xm3'Gli-BS-delta51-LucII DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Dnaconda's recommendation EXR0001j (Japanese text)
            Featured content Dnaconda's recommendation EXR0074e (English text)
            Featured content Dnaconda's recommendation EXR0074j (Japanese text)
            Featured content Promoter Collection (Outline) (English text)
            Featured content Promoter Collection (Outline) (Japanese text)
            Featured content Hedgehog signaling (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08062_A6Cep1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pGL3_RV6R
            Region: chicken delta-crystallin Basal Pro,8xm3'Gli-BS,SV40 pA
            Sequence file: RDB08062_A6Ced.seq check
            >08062_08062_A6Ce_pGL3_RV6R_E09_15.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNGC CTTTCTTTAT GTTTTTGGCG TCTTCCNTTT
               61 TACCAACAGT ACCGGAATGC CAAGCTTGGC TGCCCGCACA GCCCTGCTCA CCGTCTCCGC
              121 AGCTCAGCCC TGGCTGGTCG CTCCGTGCTC TCCGGTCTCA CAGCCCCGCT TTTACGTGCC
              181 CCGGAGCCTG GGCTGGCCCT GCAGGTCGAC TCTAGAGGAT CCCCGGGAAC AGATTCGCGA
              241 TCGAGAGCTT CTACTTCCCA CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG
              301 CTTGTCGAGA GCTTCTACTT CCCACTTCCC TGCTTGTCGA GAGCTTCTAC TTCCCACTTC
              361 CCTGCTTGTC GAGAGCTTCT ACTTCCCACT TCCCTGCTTG TCGAGAGCTT CTACTTCCCA
              421 CTTCCCTGCT TGTCGAGAGC TTCTACTTCC CACTTCCCTG CTTGTCGAGA GCTTCTACTT
              481 CCCACTTCCC TGCTTGTCGA TCGCGAATCT GTTCCCGGGG ATCCAGACAT GATAAGATAC
              541 ATTGATGANT TTGGACAAAC CACAACTAGA ATGCANTGAA AAAAATGCTT TATTTGTGAA
              601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC
              661 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
              721 TAAAACCTCT ACAAATGTGA TATGGCTGAT CCAGACATGA TAAGANACAT TGATGAGTTT
              781 GGACAAACCA CAACTAGAAT GCANTGAAAA AAATGCNTTA TTTGTGAAAT TTGTGATGCT
              841 ATTGCTTTAT NTTGTAACCN TTANNANNCT GCAATAAACA AGTTANCAAC NANTTGCNTT
              901 TCNTTTTATG TTTCNNNNTN NNGGGGGAGG TGTGGGNANG NTTTTTTNAA NNNNNNNAAA
            961 CCNNNTNNNN NGTGNNNNGN NTGNNTNCNN NNNNNNNN
            //
            Primer: RDB08068_F1
            Region: SV40 pA
            Sequence file: RDB08062_A6Cee.seq check
            >08062_08062_A6Ce_RDB8068_F1_E12_05_ABI08.ab1
                1 NNNNNNNNNN NNNGNNGGNN TTCCNNGATN NTTTAAAATT ACCCTTAGAA AGCGGTCTGT
               61 GAAAAACCCC TACCCAATTT CCTTTTTGTT AAGTGACCTA ATTAACAGGA GGACACAGAG
              121 GGTGGATGGG CAGCCTATGA TTGGAATGTC CTCTCAAGTA GAGGAGGTTA GGGTTTATGA
              181 GGACACAGAG GAGCTTCCTG GGCCTCGAGG CAGACATGAT AAGATACATT GATGAGTTTG
              241 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA
              301 TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC
              361 ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAANCNNN NNNANNNNNN
            421 NNNN
            //
            Primer: Luc-C_F
            Region: Luc 3',small t intron,SV40 NLS
            Sequence file: RDB08062_A6Cef.seq check
            >08062_08062_A6Ce_Luc-C_F_D12_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNCCNG NCNNGTAACN ACCGCGAAAA AGTTGCGCGG AGGAGTTGTG
               61 TTTGTGGACG AAGTACCGAA AGGTCTTACC GGAAAACTCG ACGCAAGAAA AATCAGAGAG
              121 ATCCTCATAA AGGCCAAGAA GGGCGGAAAG TCCAAATTGT AAAATGTAAC TGTATTCAGC
              181 GATGACGAAA TTCTTAGCTA TTGTAATACT CTAGAGGATC TTTGTGAAGG AACCTTACTT
              241 CTGTGGTGTG ACATAATTGG ACAAACTACC TACAGAGATT TAAAGCTCTA AGGTAAATAT
              301 AAAATTTTTA AGTGTATAAT GTGTTAAACT ACTGATTCTA ATTGTTTGTG TATTTTAGAT
              361 TCCAACCTAT GGAACTGATG AATGGGAGCA GTGGTGGAAT GCCTTTAATG AGGAAAACCT
              421 GTTTTGCTCA GAAGAAATGC CATCTAGTGA TGATGAGGCT ACTGCTGACT CTCAACATTC
              481 TACTCCTCCA AAAAAGAAGA GAAAGGTAGA AGACCCCAAG GACTTTCCTT CAGAATTGCT
              541 AAGTTTTTTG AGTCATGCTG TGTTTAGTAA TAGAACTCTT GCTTGCTTTG CTATTTACAC
              601 CACAAAGGAA AAAGCTGCAC TGCTATACAA GAAAATTATG GAAAAATATT CTGTAACCTT
              661 TATAAGTAGG CATAACAGTT ATAATCATAA CATACTGTTT TTTCTTACTC CACACAGGCA
              721 TAGAGTGTCT GCTATTAATA ACTATGCTCA AAAATTGTGT ANCTTTAGCT TTTTAATTTG
              781 TAAAGGGGTT AATAAGGAAT ATTTGATGTA TAGTGCCTTG NCTAGAGATC NTAATCAGCC
              841 ATACNACNTT TGTAGAGGNT TTACTTGCTT TNAAAAANNT CCCACACNTC CCCCTGAACC
              901 NGAAACATNA ANNNNATGCN NNTNNNTGNT GTTAANNTNG TTNANTGCAN CTTNNAATGG
              961 NTNANAANTA AANNNNNNCA TCNCNNNTTN NNNNAANNAA NNCNNTTTTT TTCNNTGNNN
            1021 TCNAGTNNTG GNTNNNNNNA NNNNNTCANN GNNTNNNATN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sasaki, H., A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development, 124 (7): 1313-1322 (1997). PMID 9118802.
            reference Gunarta, I.K., Critical role of glioma-associated oncogene homolog 1 in maintaining invasive and mesenchymal-like properties of melanoma cells. Cancer Sci. 108 (8): 1602-1611 (2017). PMID 28635133.
            user_report Dettmer, R., FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells. Cells 9 (9): 1927 (2020). PMID 32825270.
            user_report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014).
            user_report Wang, D.H., Hedgehog signaling regulates FOXA2 in esophageal embryogenesis and Barrett's metaplasia. J. Clin. Invest. 124 (9): 3767-80 (2014). PMID 25083987.

            2022.05.17

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