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pCMV-Flag-Tead1

Expression clone of mouse Tead1, FLAG-tag

Catalog number RDB12170
Resource name pCMV-Flag-Tead1
Clone info. Expression clone of mouse Tead1, FLAG-tag.
Vector backbone pCMV/SV2 (Plasmid)
Gene/insert name Mouse Tead1 cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Mouse Tead1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12170 pCMV-Flag-Tead1 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12170_A5Es.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12170_A5Esa.seq check
          >12170_12170_A5Es_CMV-Forward_A07_01.ab1
              1 CGTAACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTTCCACCA TGGACTACAA GGACGACGAT GACAAGGGAT
            181 CCGAATTCGA GCTCCGTCGA CAAGCTTGCG GCCGCATGGA GCCCAGCAGC TGGAGCGGCA
            241 GCGAGAGCCC TGCCGAAAAC ATGGAAAGGA TGAGCGACTC GGCAGATAAG CCGATTGACA
            301 ACGACGCGGA GGGCGTCTGG AGTCCTGATA TTGAGCAGAG TTTCCAGGAG GCCCTGGCTA
            361 TCTATCCGCC GTGTGGGAGG AGAAAAATCA TCTTATCAGA CGAAGGCAAA ATGTATGGTA
            421 GAAATGAATT GATAGCCAGA TACATCAAAC TCAGGACGGG AAAGACAAGG ACCAGGAAGC
            481 AGGTGTCTAG TCACATTCAG GTTCTTGCCA GAAGGAAATC TCGTGATTTT CATTCCAAGC
            541 TGAAGGATCA GACTGCCAAG GACAAGGCCC TGCAGCACAT GGCTGCCATG TCATCAGCCC
            601 AGATCGTCTC GGCTACTGCC ATCCACAACA AGCTGGGGCT GCCTGGGATT CCACGCCCCA
            661 CCTTCCCGGG GGGTCCGGGG TTCTGGCCTG GGATGATACA GACAGGACAG CCAGGATCCT
            721 CACAAGACGT CAAGCCCTTT GTGCAGCAGG CCTACCCCAT CCAGCCAGCA GTCACAGCCC
            781 CCATTCCAGG GTTTGAGCCT ACGTCAGCCC CAGCCCCCTC AGTTCCTGCC TGGCAGGGCC
            841 GATCCATTGG CACAACCAAG CTTCGCCTGG TGGAATTCTC CGCTTTCCTT GAACAGCAGA
            901 GAGACCCAGA CTCGTACAAC AAACACCTCT TCGTGCACAT CGGGCATGCC AACCATTCTT
            961 ACAGTGACCC GTTGCTCGAA TCTGTGGACA TTCGTCAGAT ATATGACAAA TTTCCCTGAA
           1021 AAGAAAGGTG GCTTGAAAGG AGCTGTTTGG AAAGGGGCCC CTCAAAACGC CCTTTCCTTC
           1081 TCGTCAAATT CTGGGCGGGA ACCTAACTGC CATATCTAGG ACGACGGCCG GGGCCTTTTA
          1141 ATGGGTGGTG GAAGCCAAGT TCCAGT
          //
          Primer: pMAMneo-Reverse
          Sequence file: RDB12170_A5Esb.seq check
          >12170_12170_A5Es_pMAMneo-Reverse_B07_04.ab1
              1 TTGTATATGT CACACCACAG AAGTAAGGTT CCTTCACAAA GATCCTCTAG CATTTAGGTG
             61 ACACTATAGA ATAGGGCCCT CTAGATCAGT CCTTCACAAG CCTGTAGATA TGGTGCTGTG
            121 CTCCGTGTTC GCTATTCGAG ACTTCAAATA CACAGGCCAT GCAGAGCAGA GTTTCTTGTG
            181 TATCCCTGTT TGTTACCACC AATAATATGG TGAAGTTTTC CAAAACACTG TTCATCATAT
            241 ATTTCTCTGG TAGGTGTTTG AGCTTGTGGA TGAAGTTGAT CATATATTCA CACATTGGCG
            301 AGCGGTTTAT TCGGTACACG AATCGACCAT TCTCGAACCT CGCATACTCC GTCTCTACTT
            361 TTTCTACTAC TTGTTTCCCA AAGGAGCACA CTTTGGTGGA ACAGGTAACT GTCATGTTCT
            421 CAGAACTCTC ATACTGACTG CTCACACCAT AAAAGGCCCC GGCGTCGTCT TGGATATTGC
            481 AGTTTAAGTC CGCCCAGAAT TTGACGAGGA AGAAGGCGTT TTGAGGGCCC TTTCCAAACA
            541 GCTCCTTCAA GCCACCTTTC TTTTCAGGAA ATTTGTCATA TATCTGACGA ATGTCCACAG
            601 ATTCGAGCAA CGGGTCACTG TAAGAATGGT TGGCATGCCC GATGTGCACG AAGAGGTGTT
            661 TGTTGTACGA GTCTGGGTCT CTCTGCTGTT CAAGGAAAGC GGAGAATTCC ACCAGGCGAA
            721 GCTTGGTTGT GCCAATGGAT CGGCCCTGCC AGGCAGGAAC TGAGGGGGCT GGGGCTGACG
            781 TAGGCTCAAA CCCTGGAATG GGGGCTGTGA CTGCTGGCTG GATGGGGTAG GCCTGCTGCA
            841 CAAAGGGCTT GACGTCTTGT GAGGATCCTG GCTGTCCTGT CTGTATCATC CCAGGCCAGA
            901 ACCCCGGACC CCCCGGGAAG GTGGGGCGTG GAATCCCAGG CAGCCCCAGC TTGTTGTGGA
            961 TGGCAGTAGC CGAGACGATC TGGGCTGATG ACATGGCAGC CATGTGCTGC AGGGCCTTGT
           1021 CCTTGGCAGT CTGATCCTTC AGCTTGGATG AATCACGAGA TTCTTCTGGC AGATCTTGAT
           1081 GTGACTAGAC ACTTGCCTCC TGGTCCTGTC GTGCCGTCCT GAGTGGAATG TATCTGGCCT
          1141 ATCAATTTCA ATTTTCCTTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl