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pCMV-Flag-Tead1

Expression clone of mouse Tead1, FLAG-tag

Catalog number RDB12170
Resource name pCMV-Flag-Tead1
Clone info. Expression clone of mouse Tead1, FLAG-tag.
Vector backbone pCMV/SV2 (Plasmid)
Gene/insert name Mouse Tead1 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Tead1 (NCBI Gene 21676) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12170 pCMV-Flag-Tead1 DNA solution

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References and tips

Electronic file

Featured content

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12170_A5Es.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12170_A5Esa.seq
>12170_12170_A5Es_CMV-Forward_A07_01.ab1
    1 CGTAACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTTCCACCA TGGACTACAA GGACGACGAT GACAAGGGAT
  181 CCGAATTCGA GCTCCGTCGA CAAGCTTGCG GCCGCATGGA GCCCAGCAGC TGGAGCGGCA
  241 GCGAGAGCCC TGCCGAAAAC ATGGAAAGGA TGAGCGACTC GGCAGATAAG CCGATTGACA
  301 ACGACGCGGA GGGCGTCTGG AGTCCTGATA TTGAGCAGAG TTTCCAGGAG GCCCTGGCTA
  361 TCTATCCGCC GTGTGGGAGG AGAAAAATCA TCTTATCAGA CGAAGGCAAA ATGTATGGTA
  421 GAAATGAATT GATAGCCAGA TACATCAAAC TCAGGACGGG AAAGACAAGG ACCAGGAAGC
  481 AGGTGTCTAG TCACATTCAG GTTCTTGCCA GAAGGAAATC TCGTGATTTT CATTCCAAGC
  541 TGAAGGATCA GACTGCCAAG GACAAGGCCC TGCAGCACAT GGCTGCCATG TCATCAGCCC
  601 AGATCGTCTC GGCTACTGCC ATCCACAACA AGCTGGGGCT GCCTGGGATT CCACGCCCCA
  661 CCTTCCCGGG GGGTCCGGGG TTCTGGCCTG GGATGATACA GACAGGACAG CCAGGATCCT
  721 CACAAGACGT CAAGCCCTTT GTGCAGCAGG CCTACCCCAT CCAGCCAGCA GTCACAGCCC
  781 CCATTCCAGG GTTTGAGCCT ACGTCAGCCC CAGCCCCCTC AGTTCCTGCC TGGCAGGGCC
  841 GATCCATTGG CACAACCAAG CTTCGCCTGG TGGAATTCTC CGCTTTCCTT GAACAGCAGA
  901 GAGACCCAGA CTCGTACAAC AAACACCTCT TCGTGCACAT CGGGCATGCC AACCATTCTT
  961 ACAGTGACCC GTTGCTCGAA TCTGTGGACA TTCGTCAGAT ATATGACAAA TTTCCCTGAA
 1021 AAGAAAGGTG GCTTGAAAGG AGCTGTTTGG AAAGGGGCCC CTCAAAACGC CCTTTCCTTC
 1081 TCGTCAAATT CTGGGCGGGA ACCTAACTGC CATATCTAGG ACGACGGCCG GGGCCTTTTA
1141 ATGGGTGGTG GAAGCCAAGT TCCAGT
//
Primer: pMAMneo-Reverse RDB12170_A5Esb.seq
>12170_12170_A5Es_pMAMneo-Reverse_B07_04.ab1
    1 TTGTATATGT CACACCACAG AAGTAAGGTT CCTTCACAAA GATCCTCTAG CATTTAGGTG
   61 ACACTATAGA ATAGGGCCCT CTAGATCAGT CCTTCACAAG CCTGTAGATA TGGTGCTGTG
  121 CTCCGTGTTC GCTATTCGAG ACTTCAAATA CACAGGCCAT GCAGAGCAGA GTTTCTTGTG
  181 TATCCCTGTT TGTTACCACC AATAATATGG TGAAGTTTTC CAAAACACTG TTCATCATAT
  241 ATTTCTCTGG TAGGTGTTTG AGCTTGTGGA TGAAGTTGAT CATATATTCA CACATTGGCG
  301 AGCGGTTTAT TCGGTACACG AATCGACCAT TCTCGAACCT CGCATACTCC GTCTCTACTT
  361 TTTCTACTAC TTGTTTCCCA AAGGAGCACA CTTTGGTGGA ACAGGTAACT GTCATGTTCT
  421 CAGAACTCTC ATACTGACTG CTCACACCAT AAAAGGCCCC GGCGTCGTCT TGGATATTGC
  481 AGTTTAAGTC CGCCCAGAAT TTGACGAGGA AGAAGGCGTT TTGAGGGCCC TTTCCAAACA
  541 GCTCCTTCAA GCCACCTTTC TTTTCAGGAA ATTTGTCATA TATCTGACGA ATGTCCACAG
  601 ATTCGAGCAA CGGGTCACTG TAAGAATGGT TGGCATGCCC GATGTGCACG AAGAGGTGTT
  661 TGTTGTACGA GTCTGGGTCT CTCTGCTGTT CAAGGAAAGC GGAGAATTCC ACCAGGCGAA
  721 GCTTGGTTGT GCCAATGGAT CGGCCCTGCC AGGCAGGAAC TGAGGGGGCT GGGGCTGACG
  781 TAGGCTCAAA CCCTGGAATG GGGGCTGTGA CTGCTGGCTG GATGGGGTAG GCCTGCTGCA
  841 CAAAGGGCTT GACGTCTTGT GAGGATCCTG GCTGTCCTGT CTGTATCATC CCAGGCCAGA
  901 ACCCCGGACC CCCCGGGAAG GTGGGGCGTG GAATCCCAGG CAGCCCCAGC TTGTTGTGGA
  961 TGGCAGTAGC CGAGACGATC TGGGCTGATG ACATGGCAGC CATGTGCTGC AGGGCCTTGT
 1021 CCTTGGCAGT CTGATCCTTC AGCTTGGATG AATCACGAGA TTCTTCTGGC AGATCTTGAT
 1081 GTGACTAGAC ACTTGCCTCC TGGTCCTGTC GTGCCGTCCT GAGTGGAATG TATCTGGCCT
1141 ATCAATTTCA ATTTTCCTTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl