Resource data sheet
Prev.

pMYs-HA-Yap1 DTead BD-IRES-EGFP

Retroviral vector of mouse Yap1, deletion of Tead BD

Catalog number RDB12178
Resource name pMYs-HA-Yap1 DTead BD-IRES-EGFP
Clone info. Retroviral vector of mouse Yap1, deletion of Tead binding domain (BD). cDNA fragments 210 to 299 nt and 513 to 1,625 nt of NM_009534.3 (CDS, 207..1625) are cloned (deletion of Tead binding domain).
Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). G to A substitution at 807 nt (Ala to Thr at 201st aa).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Yap1 (NCBI Gene 22601) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Schedule A (GFP-A) [Link]
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Please use Schedule A (GFP-A) additionally.
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RDB12178 pMYs-HA-Yap1 DTead BD-IRES-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12178_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') RDB12178_A6Aja.seq
>12178_12178_A6Aj_5'Retro(pFB-5')_A10_01.ab1
    1 CGCCGCTAGG GGGTTTTTTC TTCGGACTGC TAGTTATTAA GGATCCATGT ACCCATACGA
   61 CGTTCCAGAT TACGCTGGTA CCGACCCCGC GCAACAGCCG CCGCCCCAGC CGGCCCCGCA
  121 AGGCCCCGCG CCGCCGTCCG TGTCTCCGGC CGGGACCCCC GCGGCCCCGC CCCTGACTCC
  181 ACAGCATGTT CGAGCTCACT CCTCTCCAGC CTCCCTGCAG CTGGGTGCCG TTTCTCCTGG
  241 GACACTCACA GCCAGTGGCG TTGTCTCTGG CCCTGCCGCT GCCCCTGCAG CTCAGCATCT
  301 CCGGCAGTCC TCCTTTGAGA TCCCTGATGA TGTACCACTG CCAGCAGGCT GGGAGATGGC
  361 CAAGACATCT TCTGGTCAAA GATACTTCTT AAATCACAAC GATCAGACAA CAACATGGCA
  421 GGACCCCCGG AAGGCCATGC TTTCGCAACT GAACGTTCCT GCGCCTACCA GCCCAGCGGT
  481 GCCCCAGACG CTGATGAATT CTGCCTCAGG ACCTCTTCCT GATGGATGGG AGCAAGCCAT
  541 GACTCAGGAT GGAGAAGTTT ACTACATAAA CCATAAGAAC AAGACCACAT CCTGGCTGGA
  601 CCCAAGGCTG GACCCTCGTT TTGCCATGAA CCAGAGGATC ACTCAGAGTG CTCCAGTGAA
  661 GCAGCCCCCA CCCTTGGCTC CCCAGAGCCC ACAGGGAGGC GTCCTGGGTG GAGGCAGTTC
  721 CAACCAGCAG CAGCAAATAC AGCTGCAGCA GTTACAGATG GAGAAGGAGA GACTGCGGTT
  781 GAAACAACAG GAATTATTTC GGCAGGAATT AGCTCTGCGC AGCCAGTTGC CTACACTGGA
  841 GCAGGATGGA GGGACTCCGA ATGCAGTGTC TTCTCCTGGG ATGTCTCAGG AATTGAGAAC
  901 AATGACAACC AATAGTTCCG ATCCCTTTCT TAACAGTGGC ACCTATCACT CTCGAGATGA
  961 GAGCACAGAC AGCGGCCTCA GCATGAGCAG CTACAGCATC CCTTCGGGAC CCCAGACGAC
 1021 TTTCTCACAG TGTGATGAAA TGGATACAGG AGAACATCAT CAGCAAGCAC CCTTGCCGTC
 1081 ACAGCAGAGT CGCTTCCCGA CTACCTGGAA GCTCTGGAAC AATGTGAACC TTGGCCACCA
1141 CCTGGAAAAG GAC
//
Primer: IRES-R RDB12178_A6Ajb.seq
>12178_12178_A6Aj_IRES-R_C08_08.ab1
    1 GCATGGCGGG GCTCGGCCAG TACGTTAGGG GGGGGGGAGG GAGAGGGGGG GGGGGGGCGG
   61 AATTTACGTA GCGGCCGCCT ATAACCACGT GAGAAAGCTT TCTTTATCTA GCTTGGTGGC
  121 AGCCAACACA GACTCCACGT CCAAGATTTC GGAACTCAGC GCTTCCTGCA GACTGGGCAT
  181 CAGCTCCTCC CCTTCTATGT TCATTGCATC TCCTTCCAGT GTGCCAAGGT CCACATTTGT
  241 CCCAGGGAGG GCTTCCAGGT AGTCGGGGAA GCGGCTCTGC TGTGACGGCA GGGTGCTTTG
  301 GCTGATGGTG TCTCCTGTAT CCATTTCATC CACACTGTTG AGGAAGTCGT CTGGGGTCCG
  361 AGGGATGCTG TAGCTGCTCA TGCTGAGGCC GCTGTCTGTG CTCTCATCTC GAGAGTGATA
  421 GGTGCCACTG TTAAGAAAGG GATCGGAACT ATTGGTTGTC ATTGTTCTCA ATTCCTGAGA
  481 CATCCCAGGA GAAGACACTG CATTCGGAGT CCCTCCATCC TGCTCCAGTG TAGGCAACTG
  541 GCTGCGCAGA GCTAATTCCT GCCGAAATAA TTCCTGTTGT TTCAACCGCA GTCTCTCCTT
  601 CTCCATCTGT AACTGCTGCA GCTGTATTTG CTGCTGCTGG TTGGAACTGC CTCCACCCAG
  661 GACGCCTCCC TGTGGGCTCT GGGGAGCCAA GGGTGGGGGC TGCTTCACTG GAGCACTCTG
  721 AGTGATCCTC TGGTTCATGG CAAACGAGGG TCCAGCCTTG GGTCCAGCCA GGATGTGGTC
  781 TTGTTCTATG GTTATGTAGT AACTTCTCCA TCCTGGAGTC ATGCTGCTCC CATCATCAGA
  841 AGAGGTCTGA GCAGAATCAT CACGTTCTGG GGCACCCGCT GGCCTGTAGG CCAGAACGTC
  901 AGTGCGAGCC ATGGCCTCGG GTCCTGCATG TGTGTCTGGA TCGTTGGGAT CAGAGGATCT
  961 CGACGAAGTG TCTGGCATTC AGCTGTGCAT GTACTTCAGG ATCCAGAACT GCGAATGCTC
1021 GA
//
Primer: IRES-F RDB12178_A6Ajc.seq
>12178_12178_A6Aj_IRES-F_B10_04.ab1
    1 GGCAATGCCT TTTAATGTGT TTAGTCGAGG TTAAAAAACG TCTAGGCCCC CCGAACCACG
   61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
  121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
  601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
  661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
  721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
  781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGGCCG
  841 CCAGCACAGT GGTCGACCGG GCCGCCAGCA CAGTGGTCGA TCGAGGGGGG GCCCGCGATT
  901 AGTCCAATTT GTTAAAGACA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA
  961 TCACCAGCTG AAGCCTATAG AGTACGAGCC ATAGAATAGA ATAAAAAGAT TTTAATTAGT
 1021 CCTCCAGAAA AAGGGGGGGA ATGAAAGACC CCCAACCTTG TAGTTTGGGC AAAGCTAGCT
 1081 TAGGTAACGG CCAATTTTGC AAAGGCCGAT GGAAAATACA ATTAACCTGA AGAATGAGAG
1141 AAGTTCAGAA ATTCAAGGTT TTAGAGAC
//
Primer: Amp_R RDB12178_A6Ajd.seq
>12178_12178_A6Aj_Amp_R_D08_11.ab1
    1 ATCTTTTTAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT
  661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT
  721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG
  781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG
  841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC
  901 CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAAGGG
  961 ACTTCGGGGG CCGTTTTTTG TGGCCCGGAC CTGAAGTCCC AAAATTCCCG ATCGTTTTGG
 1021 ACTTCTTTGG TGCACCCCTA TAGGAGGGAT ATGGTGGTTC TGGGTAGAGA CGAAGAACTT
1081 AAACAGTCCC GGCCTCCGTC CTGAATTATT GCTTTTCGAG TTTGGGGAAC CGGAAAAGG
//
Primer: pMFG_1 RDB12178_A6Aje.seq
>12178_12178_A6Aj_pMFG-1_E08_14.ab1
    1 GCGGGGAGAG GGCTGTTAGT ACTCTTGTCA TGTCAGGGTC AGGGAAGTTT ACAAGGGGCG
   61 GGGGTGCCCC ATATAAGATC TCATATGGCC ATATGGGGGC GCCTAGAGAA GGAGTGAGGG
  121 CTGGATAAAG GGAGGATCGA GGCGGGGTCG AACGAGGAGG TTCAAGGGGG AGAGACGGGG
  181 CGGATGGAGG AAGAGGAGGC GGAGGCTTAG GGTGTACAAA GGGCTTGACC CAGGGAGGGG
  241 GGTCAAAAGC CAAGGCTTCC CAGGTCACGA TGTAGGGGAC CTGGTCTGGG TGTCCATGCG
  301 GGCCAGGTGA AAAGACCTTG ATCTTAACCT GGGTGATGAG GTCTCGGTTA AAGGTGCCGT
  361 CTCGCGGCCA TCCGACGTTA AAGGTTGGCC ATTCTGCAGA GCAGAAGGTA ACCCAACGTC
  421 TCTTCTTGAC ATCTACCGAC TGGTTGTGAG CGATCCGCTC GACATCTTTC CAGTGACCTA
  481 AGGTCAAACT TAAGGGAGTG GTAACAGTCT GGCCCTAATT TTCAGACAAA TACAGAAACA
  541 CAGTCAGACA GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC
  601 GGTCCCAAAC CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC
  661 CTACCAGAAC CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG
  721 GATTTTTGGA CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA
  781 GGGTTGCGGC CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC
  841 TAGTTAGCTA ACTAGTACAG ACGCAGGCGC AAACATTAGA TGCCGGCACA AACACGCACA
  901 AAGACAGAGA CAGACACGAA ACGACCGCTG GGCCAGCTTA CCTCCCGGCG GGGGGGTCGG
  961 TGGTCCCTGG GCAGGGGTCT CCAATCTCGG TGGACTCGAA ATGAAGACCC CCGAGTGGGC
 1021 AGTCCATTCA TCTGAAGAGA CCTCCAAGAT CAGCGAGTTC ACGATCAGAT GCACAGCAGA
1081 GCCTTTATGG AATACGGTTA CCCGGGCGCA
//
Primer: EGFP-C RDB12178_A6Ajf.seq
>12178_12178_A6Aj_EGFP-C_F08_17.ab1
    1 CAGGGCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG
   61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT
  121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA
  181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG
  241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA
  301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA
  361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC
  421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT
  481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC
  541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT
  601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC
  661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC
  721 GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT
  781 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCACATGCAG
  841 CATGTATCAA AATTAATTTG GTTTTTTTTC TTAAGTATTT ACATTAAATG GCCATAGTTG
  901 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT
  961 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGG TATCAGCTCA
 1021 CTCAAAGGCG GGTAATACGG GTTATCCCAC AGAATTCAGG GGATTACGCA GAAAAGAACA
1081 TGTGGAGCCA AACGCCCAGG CCAAAGGGCC AGGAACCGTA AAAAAAAGGC TGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl