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pMYs-Tead2-VP16-IRES-EGFP

Retroviral vector of mouse Tead2, constitutive active

Catalog number RDB12174
Resource name pMYs-Tead2-VP16-IRES-EGFP
Clone info. Retroviral vector of mouse Tead2, activation domain of herpes simplex virus VP16, constitutive active. cDNA fragment 87 to 1,424 nt of NM_011565.2 (CDS, 87..1424) is cloned.
Comment Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to A substitution at 453 nt (Val to Ile at 123rd aa).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.2 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA HSV VP16 protein cDNA
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank

External Database
Mouse Tead2 HSV VP16 protein

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks Remember that you will be working with samples containing infections virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          付加的提供条件 pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12174 pMYs-Tead2-VP16-IRES-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12174_A4Jmp1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: 5'Retro(pFB-5')
          Region: insert5'(mTead2,VP16)
          Sequence file: RDB12174_A4Jma.seq check
          >12174_12174_A4Jm_5'Retro(pFB-5')_G04_19.ab1
              1 CAACTGTCAT GCACGTTACT AGCGTCGTTT AGCGTCTCGG ACTGCTGTTA TTAGGATCCC
             61 AGTGTGGTGG TACGGGAATT CCTGCAGGAG CTATTTAGGT GACACTATAG AATACAAGCT
            121 ACTTGTTCTT TTTGCAGGAT CCCATCGATT CGAATTCGAG GCCCAGATGG GGGATCCCCG
            181 GACTGGGGCC CCCCTGGATG ATGGCGGGGG CTGGACAGGT AGCGAGGAAG GCAGCGAAGA
            241 GGGCACCGGA GGCAGCGAGG GCGTTGGAGG TGATGGCAGC CCTGATGCAG AAGGTGTGTG
            301 GAGCCCCGAC ATTGAGCAGA GTTTTCAGGA GGCCCTGGCC ATCTACCCTC CCTGTGGCCG
            361 TCGCAAGATC ATCCTGTCAG ATGAGGGCAA GATGTATGGC CGGAACGAAC TCATTGCCCG
            421 TTACATCAAG CTGAGGACAG GGAAGACGAG AACGCGAAAG CAGGTCTCCA GCCATATTCA
            481 GGTTTTGGCT CGAAGGAAAT CGAGAGAAAT TCAGTCCAAG CTGAAGGACC AAATCTCCAA
            541 GGACAAGGAA TTCCCGGGGA TCTGGGCCCC CCCGACCGAT GTCAGCCTGG GGGACGAGCT
            601 CCACTTAGAC GGCGAGGACG TGGCGATGGC GCATGCCGAC GCGCTAGACG ATTTCGATCT
            661 GGACATGTTG GGGGACGGGG ATTCCCCGGG TCCGGGATTT ACCCCCCACG ACTCCGCCCC
            721 CTACGGCGCT CTGGATATGG CCGACTTCGA GTTTGAGCAG ATGTTTACCG ATGCCCTTGG
            781 AATTGACGAG TACGGTGGGT AGGCCTCGAG GGCCCGGCGC GCCGCGGCCG CTACGTAAAT
          841 TCGCCCCCCC CCCCCCCTTC TTCC
          //
          Primer: IRES-R
          Region: IRES,insert,MMLV gag-pol
          Sequence file: RDB12174_A4Jmb.seq check
          >12174_12174_A4Jm_IRES-R_A03_03.ab1
              1 GCACGCGGCT CGGCAGTACG TTAGGGGGGG GGGAGGGAGA GGGGGGGGGG GGGCGGAATT
             61 TACGTAGCGG CCGCGGCGCG CCGGCCCTCG AGGCCTACCC ACCGTACTCG TCAATTCCAA
            121 GGGCATCGGT AAACATCTGC TCAAACTCGA AGTCGGCCAT ATCCAGAGCG CCGTAGGGGG
            181 CGGAGTCGTG GGGGGTAAAT CCCGGACCCG GGGAATCCCC GTCCCCCAAC ATGTCCAGAT
            241 CGAAATCGTC TAGCGCGTCG GCATGCGCCA TCGCCACGTC CTCGCCGTCT AAGTGGAGCT
            301 CGTCCCCCAG GCTGACATCG GTCGGGGGGG CCCAGATCCC CGGGAATTCC TTGTCCTTGG
            361 AGATTTGGTC CTTCAGCTTG GACTGAATTT CTCTCGATTT CCTTCGAGCC AAAACCTGAA
            421 TATGGCTGGA GACCTGCTTT CGCGTTCTCG TCTTCCCTGT CCTCAGCTTG ATGTAACGGG
            481 CAATGAGTTC GTTCCGGCCA TACATCTTGC CCTCATCTGA CAGGATGATC TTGCGACGGC
            541 CACAGGGAGG GTAGATGGCC AGGGCCTCCT GAAAACTCTG CTCAATGTCG GGGCTCCACA
            601 CACCTTCTGC ATCAGGGCTG CCATCACCTC CAACGCCCTC GCTGCCTCCG GTGCCCTCTT
            661 CGCTGCCTTC CTCGCTACCT GTCCAGCCCC CGCCATCATC CAGGGGGGCC CCAGTCCGGG
            721 GATCCCCCAT CTGGGCCTCG AATTCGAATC GATGGGATCC TGCAAAAAGA ACAAGTAGCT
            781 TGTATTCTAT AGTGTCACCT AAATAGCTCC TGCAGGAATT CCCGTACCAC CACACTGGGA
            841 TCCTTAATTA ACTAGCTAGA TCCGGCAGTC TAGAGGATGG TCCACCCCCG GGGTCGGCAG
            901 CCTTCACGTG GGCGGCGTGT ATCCAAGCTG CGATGCCGTC TACTTTGAGG GCGGTGGGGG
            961 GTGGTCAGCA GGACTGTGTA AGGTCCTTTC CAGCGAGGTT CTAGGTTCTT AGTCTGGTGT
           1021 CGGCGGGACC CACACTGTGT CGCCGACTCG GTAAGGGTGA GGTACCACCG GTCGGTCCAG
           1081 CTGTTCTTGG TAGGCTGCGC CAGAGGTCTC AGACTCGTGC CTGAACTTAA GGTTAGAGGA
          1141 GTCTGTAGTG AGCTTGGAAG AAGAAGGGGG CCT
          //
          Primer: IRES-F
          Region: IRES,EGFP 5'
          Sequence file: RDB12174_A4Jmc.seq check
          >12174_12174_A4Jm_IRES_F_A02_02.ab1
              1 GGCAGCCTTT TATGTGGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC GAACCACGGG
             61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG
            121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG
            181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT
            241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT
            301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT
            361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG
            421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA
            481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA
            541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAAGCA GAAGAACGGC ATCAAAGGTG
            601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
            661 CAGAACACCC CCATCGGCGA CGGCCCCCGT GCTGCTGCCC GACAACCACT ACCCTGAGCA
            721 CCCAGTCCGC CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
            781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAGTAA AGCGGCCGGC
            841 CGCCAGCACA GTGGTCGAAC GGGGCCGCCA GCACAGTGGT CGAATCGAAG GGGGGGCCCG
            901 CGAATTAGTC CAATTTGTTA AAAGACAAGG AATATTCAAT GGTTCAAGGG CTCAATTTTG
          961 ACCTCAACCA ATATTCACCA CCTGA
          //
          Primer: Amp_R
          Region: 5'LTR,psi,
          Sequence file: RDB12174_A4Jmd.seq check
          >12174_12174_A6Aj_Amp_R_A07_01.ab1
              1 TTCGTTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
             61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
            121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
            181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
            241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
            301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
            361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
            421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
            481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
            541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
            601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT
            661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT
            721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCCGG
            781 GAGGTAAGCT GGGCCAGCGG TCGTTTCGTG TCTGTCTCTG TCTTTGTGCG TGTTTGTGCC
            841 GGCATCTATG TTTGCGCCTG CGTCTGTACT AGTAGCTTAC TAGCTCTGTA TCTGCGACCG
            901 TGTGACTGAC GAGTCGAACA CCCGCGCAAC CTTGGGAGAC GTCCAAGAAC TCGGGACGTT
            961 TTGTGCCGAC TGATCCAATC GATCGATGAC CTTAGTGCAC GCTATCGAGA TCTGATTCGG
          1021 TAGAC
          //
          Primer: pMFG_1
          Region: MMLV-gag-pol,psi
          Sequence file: RDB12174_A4Jme.seq check
          >12174_12174_A6Aj_pMFG-1_D07_10.ab1
              1 GCAGGGGGGA GGGGCTGTTA GTACTCTTGT CATGTCAGGG TCAGGGAAGT TTACAAGGGG
             61 CGGGGGTGCC CCATATAAGA TCTCATATGG CCATATGGGG GCGCCTAGAG AAGGAGTGAG
            121 GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG GGAGAGACGG
            181 GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA CCCAGGGAGG
            241 GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG GGTGTCCATG
            301 CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT TAAAGGTGCC
            361 GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG TAACCCAACG
            421 TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT TCCAGTGACC
            481 TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA AATACAGAAA
            541 CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG CGGCGCGGCT
            601 TCGGTCCCAA ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA GGTTCTCGTC
            661 TCCTACCAGA ACCACATATC CCTCCTATTA GGGGGGTGCA CCAAAGAGTC CAAAACGATC
            721 GGGATTTTTG GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG GGACGTCTCC
            781 CAGGGTTGCG GCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC CAGATACAGA
            841 GCTAGTTAGC TAACTAGTAC AGACGCAGGC GCAACATTAG ATGCCGGGCA CAACACCGCC
            901 ACAGACGAGA CGACACGAAA CGACCGCTGC CAGCCTAACC TCCCGGCGGG GGTCGTGGTC
            961 CCTGGCAAGG TCTCAATCTC GTGAACCTCA TTGAAGATCC TCGAGGTGGG AGTCATCATC
           1021 TGAGAGACCC TCCAGGATAC CGCGATTCCG ATTCGAGTGC ACCGGAACGC TTATGATCGT
          1081 ACCGGCGCAC TTGAGTCAA
          //
          Primer: EGFP-C
          Region: EGFP 3',3'LTR
          Sequence file: RDB12174_A4Jmf.seq check
          >12174_12174_A6Aj_EGFP-C_B07_04.ab1
              1 GGGGGGACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG
             61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT
            121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA
            181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG
            241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA
            301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA
            361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC
            421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT
            481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC
            541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT
            601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC
            661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC
            721 GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT
            781 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCACATGCAG
            841 CATGTATCAA AATTAATTTG GTTTTTTTTT CTTAAGTATT TACATTAAAT GGCCATAGTT
            901 GCATTAATGA ATCGGCCAAC GCGCGGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
            961 CTTCCTCGCT CACTGACTCG CTGCGCTCGG GTCGTTTCGG GCTGCGGGCG AGCGGTATCA
           1021 GCTCACTCAA GCCGGTAATA CGTTATCCCA ACAGAATCAA GGGAATACGC AAGTAAAGAA
          1081 CATGGTGACT AAGGTCAGCA AGGCCAGACC GGTAAAAAGG CCGCGCGTTG ACG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.
          user_report Zhang, J., TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma. Am. J. Pathol. pii: S0002-9440 (18) 30776-4 (2019). PMID 30794805.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl