Resource data sheet
Prev.

pMYs-Tead2-VP16-IRES-EGFP

Retroviral vector of mouse Tead2, constitutive active

Catalog number RDB12174
Resource name pMYs-Tead2-VP16-IRES-EGFP
Clone info. Retroviral vector of mouse Tead2, activation domain of herpes simplex virus VP16, constitutive active. cDNA fragment 87 to 1,424 nt of NM_011565.2 (CDS, 87..1424) is cloned.
Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to A substitution at 453 nt (Val to Ile at 123rd aa).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.2 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA HSV VP16 protein cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Tead2 (NCBI Gene 21677) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infections virus.
Please use Schedule A (GFP-A) additionally.
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RDB12174 pMYs-Tead2-VP16-IRES-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12174_A4Jmp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro(pFB-5') RDB12174_A4Jma.seq
>12174_12174_A4Jm_5'Retro(pFB-5')_G04_19.ab1
    1 CAACTGTCAT GCACGTTACT AGCGTCGTTT AGCGTCTCGG ACTGCTGTTA TTAGGATCCC
   61 AGTGTGGTGG TACGGGAATT CCTGCAGGAG CTATTTAGGT GACACTATAG AATACAAGCT
  121 ACTTGTTCTT TTTGCAGGAT CCCATCGATT CGAATTCGAG GCCCAGATGG GGGATCCCCG
  181 GACTGGGGCC CCCCTGGATG ATGGCGGGGG CTGGACAGGT AGCGAGGAAG GCAGCGAAGA
  241 GGGCACCGGA GGCAGCGAGG GCGTTGGAGG TGATGGCAGC CCTGATGCAG AAGGTGTGTG
  301 GAGCCCCGAC ATTGAGCAGA GTTTTCAGGA GGCCCTGGCC ATCTACCCTC CCTGTGGCCG
  361 TCGCAAGATC ATCCTGTCAG ATGAGGGCAA GATGTATGGC CGGAACGAAC TCATTGCCCG
  421 TTACATCAAG CTGAGGACAG GGAAGACGAG AACGCGAAAG CAGGTCTCCA GCCATATTCA
  481 GGTTTTGGCT CGAAGGAAAT CGAGAGAAAT TCAGTCCAAG CTGAAGGACC AAATCTCCAA
  541 GGACAAGGAA TTCCCGGGGA TCTGGGCCCC CCCGACCGAT GTCAGCCTGG GGGACGAGCT
  601 CCACTTAGAC GGCGAGGACG TGGCGATGGC GCATGCCGAC GCGCTAGACG ATTTCGATCT
  661 GGACATGTTG GGGGACGGGG ATTCCCCGGG TCCGGGATTT ACCCCCCACG ACTCCGCCCC
  721 CTACGGCGCT CTGGATATGG CCGACTTCGA GTTTGAGCAG ATGTTTACCG ATGCCCTTGG
  781 AATTGACGAG TACGGTGGGT AGGCCTCGAG GGCCCGGCGC GCCGCGGCCG CTACGTAAAT
841 TCGCCCCCCC CCCCCCCTTC TTCC
//
Primer: IRES-R RDB12174_A4Jmb.seq
>12174_12174_A4Jm_IRES-R_A03_03.ab1
    1 GCACGCGGCT CGGCAGTACG TTAGGGGGGG GGGAGGGAGA GGGGGGGGGG GGGCGGAATT
   61 TACGTAGCGG CCGCGGCGCG CCGGCCCTCG AGGCCTACCC ACCGTACTCG TCAATTCCAA
  121 GGGCATCGGT AAACATCTGC TCAAACTCGA AGTCGGCCAT ATCCAGAGCG CCGTAGGGGG
  181 CGGAGTCGTG GGGGGTAAAT CCCGGACCCG GGGAATCCCC GTCCCCCAAC ATGTCCAGAT
  241 CGAAATCGTC TAGCGCGTCG GCATGCGCCA TCGCCACGTC CTCGCCGTCT AAGTGGAGCT
  301 CGTCCCCCAG GCTGACATCG GTCGGGGGGG CCCAGATCCC CGGGAATTCC TTGTCCTTGG
  361 AGATTTGGTC CTTCAGCTTG GACTGAATTT CTCTCGATTT CCTTCGAGCC AAAACCTGAA
  421 TATGGCTGGA GACCTGCTTT CGCGTTCTCG TCTTCCCTGT CCTCAGCTTG ATGTAACGGG
  481 CAATGAGTTC GTTCCGGCCA TACATCTTGC CCTCATCTGA CAGGATGATC TTGCGACGGC
  541 CACAGGGAGG GTAGATGGCC AGGGCCTCCT GAAAACTCTG CTCAATGTCG GGGCTCCACA
  601 CACCTTCTGC ATCAGGGCTG CCATCACCTC CAACGCCCTC GCTGCCTCCG GTGCCCTCTT
  661 CGCTGCCTTC CTCGCTACCT GTCCAGCCCC CGCCATCATC CAGGGGGGCC CCAGTCCGGG
  721 GATCCCCCAT CTGGGCCTCG AATTCGAATC GATGGGATCC TGCAAAAAGA ACAAGTAGCT
  781 TGTATTCTAT AGTGTCACCT AAATAGCTCC TGCAGGAATT CCCGTACCAC CACACTGGGA
  841 TCCTTAATTA ACTAGCTAGA TCCGGCAGTC TAGAGGATGG TCCACCCCCG GGGTCGGCAG
  901 CCTTCACGTG GGCGGCGTGT ATCCAAGCTG CGATGCCGTC TACTTTGAGG GCGGTGGGGG
  961 GTGGTCAGCA GGACTGTGTA AGGTCCTTTC CAGCGAGGTT CTAGGTTCTT AGTCTGGTGT
 1021 CGGCGGGACC CACACTGTGT CGCCGACTCG GTAAGGGTGA GGTACCACCG GTCGGTCCAG
 1081 CTGTTCTTGG TAGGCTGCGC CAGAGGTCTC AGACTCGTGC CTGAACTTAA GGTTAGAGGA
1141 GTCTGTAGTG AGCTTGGAAG AAGAAGGGGG CCT
//
Primer: IRES_F RDB12174_A4Jmc.seq
>12174_12174_A4Jm_IRES_F_A02_02.ab1
    1 GGCAGCCTTT TATGTGGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC GAACCACGGG
   61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG
  121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG
  181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT
  241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT
  301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT
  361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG
  421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA
  481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA
  541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAAGCA GAAGAACGGC ATCAAAGGTG
  601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
  661 CAGAACACCC CCATCGGCGA CGGCCCCCGT GCTGCTGCCC GACAACCACT ACCCTGAGCA
  721 CCCAGTCCGC CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG
  781 TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAGTAA AGCGGCCGGC
  841 CGCCAGCACA GTGGTCGAAC GGGGCCGCCA GCACAGTGGT CGAATCGAAG GGGGGGCCCG
  901 CGAATTAGTC CAATTTGTTA AAAGACAAGG AATATTCAAT GGTTCAAGGG CTCAATTTTG
961 ACCTCAACCA ATATTCACCA CCTGA
//
Primer: Amp_R RDB12174_A4Jmd.seq
>12174_12174_A6Aj_Amp_R_A07_01.ab1
    1 TTCGTTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT
  661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT
  721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCCGG
  781 GAGGTAAGCT GGGCCAGCGG TCGTTTCGTG TCTGTCTCTG TCTTTGTGCG TGTTTGTGCC
  841 GGCATCTATG TTTGCGCCTG CGTCTGTACT AGTAGCTTAC TAGCTCTGTA TCTGCGACCG
  901 TGTGACTGAC GAGTCGAACA CCCGCGCAAC CTTGGGAGAC GTCCAAGAAC TCGGGACGTT
  961 TTGTGCCGAC TGATCCAATC GATCGATGAC CTTAGTGCAC GCTATCGAGA TCTGATTCGG
1021 TAGAC
//
Primer: pMFG-1 RDB12174_A4Jme.seq
>12174_12174_A6Aj_pMFG-1_D07_10.ab1
    1 GCAGGGGGGA GGGGCTGTTA GTACTCTTGT CATGTCAGGG TCAGGGAAGT TTACAAGGGG
   61 CGGGGGTGCC CCATATAAGA TCTCATATGG CCATATGGGG GCGCCTAGAG AAGGAGTGAG
  121 GGCTGGATAA AGGGAGGATC GAGGCGGGGT CGAACGAGGA GGTTCAAGGG GGAGAGACGG
  181 GGCGGATGGA GGAAGAGGAG GCGGAGGCTT AGGGTGTACA AAGGGCTTGA CCCAGGGAGG
  241 GGGGTCAAAA GCCAAGGCTT CCCAGGTCAC GATGTAGGGG ACCTGGTCTG GGTGTCCATG
  301 CGGGCCAGGT GAAAAGACCT TGATCTTAAC CTGGGTGATG AGGTCTCGGT TAAAGGTGCC
  361 GTCTCGCGGC CATCCGACGT TAAAGGTTGG CCATTCTGCA GAGCAGAAGG TAACCCAACG
  421 TCTCTTCTTG ACATCTACCG ACTGGTTGTG AGCGATCCGC TCGACATCTT TCCAGTGACC
  481 TAAGGTCAAA CTTAAGGGAG TGGTAACAGT CTGGCCCTAA TTTTCAGACA AATACAGAAA
  541 CACAGTCAGA CAGAGACAAC ACAGAACGAT GCTGCAGCAG ACAAGACGCG CGGCGCGGCT
  601 TCGGTCCCAA ACCGAAAGCA AAAATTCAGA CGGAGGCGGG AACTGTTTTA GGTTCTCGTC
  661 TCCTACCAGA ACCACATATC CCTCCTATTA GGGGGGTGCA CCAAAGAGTC CAAAACGATC
  721 GGGATTTTTG GACTCAGGTC GGGCCACAAA AACGGCCCCC GAAGTCCCTG GGACGTCTCC
  781 CAGGGTTGCG GCCGGGTGTT CCGAACTCGT CAGTTCCACC ACGGGTCCGC CAGATACAGA
  841 GCTAGTTAGC TAACTAGTAC AGACGCAGGC GCAACATTAG ATGCCGGGCA CAACACCGCC
  901 ACAGACGAGA CGACACGAAA CGACCGCTGC CAGCCTAACC TCCCGGCGGG GGTCGTGGTC
  961 CCTGGCAAGG TCTCAATCTC GTGAACCTCA TTGAAGATCC TCGAGGTGGG AGTCATCATC
 1021 TGAGAGACCC TCCAGGATAC CGCGATTCCG ATTCGAGTGC ACCGGAACGC TTATGATCGT
1081 ACCGGCGCAC TTGAGTCAA
//
Primer: EGFP-C RDB12174_A4Jmf.seq
>12174_12174_A6Aj_EGFP-C_B07_04.ab1
    1 GGGGGGACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG
   61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT
  121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA
  181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG
  241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA
  301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA
  361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC
  421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT
  481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC
  541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT
  601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC
  661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC
  721 GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT
  781 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCACATGCAG
  841 CATGTATCAA AATTAATTTG GTTTTTTTTT CTTAAGTATT TACATTAAAT GGCCATAGTT
  901 GCATTAATGA ATCGGCCAAC GCGCGGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
  961 CTTCCTCGCT CACTGACTCG CTGCGCTCGG GTCGTTTCGG GCTGCGGGCG AGCGGTATCA
 1021 GCTCACTCAA GCCGGTAATA CGTTATCCCA ACAGAATCAA GGGAATACGC AAGTAAAGAA
1081 CATGGTGACT AAGGTCAGCA AGGCCAGACC GGTAAAAAGG CCGCGCGTTG ACG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.
user report Zhang, J., TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma. Am. J. Pathol. pii: S0002-9440 (18) 30776-4 (2019). PMID 30794805.

2019.08.05

GNP_filter3_RDBDEP_html_190432.pl