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delta51-LucII

Reporter plasmid of chicken delta-crystallin basal promoter

Catalog number RDB08068
Resource name delta51-LucII
Clone info. Reporter plasmid of chicken delta-crystallin basal promoter.
Use empty control of 8x3'Gli-BS-delta51-LucII (cat.# RDB08061) and 8xGT-IIC-LucII (cat.# RDB08067).
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Sasaki, Hiroshi | Kondoh, Hisato |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 13, 5206-5215 (1993).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08068 delta51-LucII DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Promoter Collection (Outline) (English text)
Featured content Promoter Collection (Outline) (Japanese text)
Featured content Hippo signaling (English text)
Featured content Hedgehog signaling (English text)

References

original Kamachi, Y., Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer. Mol. Cell. Biol., 13 (9): 5206-5215 (1993). PMID 8355679.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08068_A8Ipp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3-RV6R RDB08068_A8Ipa.seq
>08068_08068_A8Ip_pGL3_RV6R_A10_01_ABI24.ab1
    1 CTAAGGAAAG GTATAGTAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATTT
   61 TACCAACAGT ACCGGAATGC CAAGCTTGGC TGCCCGCACA GCCCTGCTCA CCGTCTCCGC
  121 AGCTCAGCCC TGGCTGGTCG CTCCGTGCTC TCCGGTCTCA CAGCCCCGCT TTTACGTGCC
  181 CCGGAGCCTG GGCTGGCCCT GCAGGTCGAC TCTAGAGGAT CCAGACATGA TAAGATACAT
  241 TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT
  301 TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA
  361 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA
  421 AAACCTCTAC AAATGTGATA TGGCTGATCC AGACATGATA AGATACATTG ATGAGTTTGG
  481 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT
  541 TGCTTTATTT GTAACCATTA TAAGCTGCAT AAACAAGTTA ACATCAATTG CATTCATTTT
601 ATGTTTCGGT TCAGGGGGAG GTGTGGGGAG GT
//
Primer: Luc-C_F RDB08068_A8Ipb.seq
>08068_08068_A8Ip_Luc-C_F_C10_07_ABI24.ab1
    1 TGAATCGGTC CAGTCAAGTA ACAACCGCGA AAAGTTGCGC GGAGGAGTTG TGTTTGTGGA
   61 CGAAGTACCG AAAGGTCTTA CCGGAAAACT CGACGCAAGA AAAATCAGAG AGATCCTCAT
  121 AAAGGCCAAG AAGGGCGGAA AGTCCAAATT GTAAAATGTA ACTGTATTCA GCGATGACGA
  181 AATTCTTAGC TATTGTAATA CTCTAGAGGA TCTTTGTGAA GGAACCTTAC TTCTGTGGTG
  241 TGACATAATT GGACAAACTA CCTACAGAGA TTTAAAGCTC TAAGGTAAAT ATAAAATTTT
  301 TAAGTGTATA ATGTGTTAAA CTACTGATTC TAATTGTTTG TGTATTTTAG ATTCCAACCT
  361 ATGGAACTGA TGAATGGGAG CAGTGGTGGA ATGCCTTTAA TGAGGAAAAC CTGTTTTGCT
  421 CAGAAGAAAT GCCATCTAGT GATGATGAGG CTACTGCTGA CTCTCAACAT TCTACTCCTC
  481 CAAAAAAGAA GAGAAAGGTA GAAGACCCCA AGGACTTTCC TTCAGAATTG CTAAGTTTTT
  541 TGAGTCATGC TGTGTTTAGT AATAGAACTC TTGCTTGCTT TGCTATTTAC ACCACAAAGG
  601 AAAAAGCTGC ACTGCTATAC AAGAAAATTA TGGAAAAATA TTCTGTAACC TTTATAAGTA
  661 GGCATAACAG TTATAATCAT AACATACTGT TTTTTCTTAC TCCACACAGG CATAGAGTGT
  721 CTGCTATTAA TAACTATGCT CAAAAATTGT GTACCTTTAG CTTTTTAATT TGTAAAGGGG
  781 TTAATAAGGA ATATTTGATG TATAGTGCCT TGACTAGAGA TCATAATCAG CCATACCACA
  841 TTTGTAGAGG TTTTACTTGC TTTAAAAAAA CCTCCCACAC CTCCCCCCTG AAACCTGAAA
  901 CATAAAATGA ATGCAATTGT TGTTGTTAAA CTTTGTTTAT TGCAGCTTAT AATGGTTTAC
  961 AATAAAGCAA ATAGCCATCA CAAATTTTCA CAAATAAAGC ATTTTTTTTT TCACTGCATT
 1021 CTAGTTGTGG ATTTGACGAA CTCAATCCAA TGTATCTATC CATGGTCTGC ATTCGAAAGG
1081 GTCCTCAGGA AAGCCTTC
//
Primer: RDB08068_F1 RDB08068_A8Ipc.seq
>08068_08068_A8Ip_RDB8068_F1_F12_06_ABI08.ab1
    1 TCAGGGCAGT GGAAGGGACT TCCCAGATAT TTTAAAATTA CCCTTAGAAA GCGGTCTGTG
   61 AAAAACCCCT ACCCAATTTC CTTTTTGTTA AGTGACCTAA TTAACAGGAG GACACAGAGG
  121 GTGGATGGGC AGCCTATGAT TGGAATGTCC TCTCAAGTAG AGGAGGTTAG GGTTTATGAG
  181 GACACAGAGG AGCTTCCTGG GCCTCGAGGC AGACATGATA AGATACATTG ATGAGTTTGG
  241 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT
  301 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA
361 TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kamachi, Y., Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer. Mol. Cell. Biol., 13 (9): 5206-5215 (1993). PMID 8355679.

2019.03.07

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