RDB08068 - delta51-LucII

Resource data sheet
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delta51-LucII

Reporter plasmid of chicken delta-crystallin basal promoter

Catalog number	RDB08068
Resource name	delta51-LucII
Clone info.	Reporter plasmid of chicken delta-crystallin basal promoter. Use empty control of 8x3'Gli-BS-delta51-LucII (cat.# RDB08061) and 8xGT-IIC-LucII (cat.# RDB08067).
Vector backbone	delta51-LucII (Plasmid)
Size of vector backbone	6.1 kb
Selectable markers	Amp^r
Gene/insert name	firefly (Photinus pyralis) luciferase cDNA
Depositor	Sasaki, Hiroshi \| Kondoh, Hisato \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 13, 5206-5215 (1993).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 13, 5206-5215 (1993).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Cell. Biol., 13, 5206-5215 (1993).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08068	delta51-LucII	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content	Promoter Collection (Outline) (English text)
Featured content	Promoter Collection (Outline) (Japanese text)
Featured content	Hippo signaling (English text)
Featured content	Hedgehog signaling (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB08068_A8Ipp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3-RV6R	RDB08068_A8Ipa.seq
>08068_08068_A8Ip_pGL3_RV6R_A10_01_ABI24.ab1 1 CTAAGGAAAG GTATAGTAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATTT 61 TACCAACAGT ACCGGAATGC CAAGCTTGGC TGCCCGCACA GCCCTGCTCA CCGTCTCCGC 121 AGCTCAGCCC TGGCTGGTCG CTCCGTGCTC TCCGGTCTCA CAGCCCCGCT TTTACGTGCC 181 CCGGAGCCTG GGCTGGCCCT GCAGGTCGAC TCTAGAGGAT CCAGACATGA TAAGATACAT 241 TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT 301 TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA 361 TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA 421 AAACCTCTAC AAATGTGATA TGGCTGATCC AGACATGATA AGATACATTG ATGAGTTTGG 481 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT 541 TGCTTTATTT GTAACCATTA TAAGCTGCAT AAACAAGTTA ACATCAATTG CATTCATTTT 601 ATGTTTCGGT TCAGGGGGAG GTGTGGGGAG GT //
Primer: Luc-C_F	RDB08068_A8Ipb.seq
>08068_08068_A8Ip_Luc-C_F_C10_07_ABI24.ab1 1 TGAATCGGTC CAGTCAAGTA ACAACCGCGA AAAGTTGCGC GGAGGAGTTG TGTTTGTGGA 61 CGAAGTACCG AAAGGTCTTA CCGGAAAACT CGACGCAAGA AAAATCAGAG AGATCCTCAT 121 AAAGGCCAAG AAGGGCGGAA AGTCCAAATT GTAAAATGTA ACTGTATTCA GCGATGACGA 181 AATTCTTAGC TATTGTAATA CTCTAGAGGA TCTTTGTGAA GGAACCTTAC TTCTGTGGTG 241 TGACATAATT GGACAAACTA CCTACAGAGA TTTAAAGCTC TAAGGTAAAT ATAAAATTTT 301 TAAGTGTATA ATGTGTTAAA CTACTGATTC TAATTGTTTG TGTATTTTAG ATTCCAACCT 361 ATGGAACTGA TGAATGGGAG CAGTGGTGGA ATGCCTTTAA TGAGGAAAAC CTGTTTTGCT 421 CAGAAGAAAT GCCATCTAGT GATGATGAGG CTACTGCTGA CTCTCAACAT TCTACTCCTC 481 CAAAAAAGAA GAGAAAGGTA GAAGACCCCA AGGACTTTCC TTCAGAATTG CTAAGTTTTT 541 TGAGTCATGC TGTGTTTAGT AATAGAACTC TTGCTTGCTT TGCTATTTAC ACCACAAAGG 601 AAAAAGCTGC ACTGCTATAC AAGAAAATTA TGGAAAAATA TTCTGTAACC TTTATAAGTA 661 GGCATAACAG TTATAATCAT AACATACTGT TTTTTCTTAC TCCACACAGG CATAGAGTGT 721 CTGCTATTAA TAACTATGCT CAAAAATTGT GTACCTTTAG CTTTTTAATT TGTAAAGGGG 781 TTAATAAGGA ATATTTGATG TATAGTGCCT TGACTAGAGA TCATAATCAG CCATACCACA 841 TTTGTAGAGG TTTTACTTGC TTTAAAAAAA CCTCCCACAC CTCCCCCCTG AAACCTGAAA 901 CATAAAATGA ATGCAATTGT TGTTGTTAAA CTTTGTTTAT TGCAGCTTAT AATGGTTTAC 961 AATAAAGCAA ATAGCCATCA CAAATTTTCA CAAATAAAGC ATTTTTTTTT TCACTGCATT 1021 CTAGTTGTGG ATTTGACGAA CTCAATCCAA TGTATCTATC CATGGTCTGC ATTCGAAAGG 1081 GTCCTCAGGA AAGCCTTC //
Primer: RDB08068_F1	RDB08068_A8Ipc.seq
>08068_08068_A8Ip_RDB8068_F1_F12_06_ABI08.ab1 1 TCAGGGCAGT GGAAGGGACT TCCCAGATAT TTTAAAATTA CCCTTAGAAA GCGGTCTGTG 61 AAAAACCCCT ACCCAATTTC CTTTTTGTTA AGTGACCTAA TTAACAGGAG GACACAGAGG 121 GTGGATGGGC AGCCTATGAT TGGAATGTCC TCTCAAGTAG AGGAGGTTAG GGTTTATGAG 181 GACACAGAGG AGCTTCCTGG GCCTCGAGGC AGACATGATA AGATACATTG ATGAGTTTGG 241 ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT 301 TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA 361 TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT //

Please visit Sequencing and PCR primers for primer information.

References

original	Kamachi, Y., Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer. Mol. Cell. Biol., 13 (9): 5206-5215 (1993). PMID 8355679.

2019.03.07

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