Resource data sheet
Prev.

pCMV/SV-Flag-Lats2

Expression clone of mouse Lats2

Catalog number RDB12213
Resource name pCMV/SV-Flag-Lats2
Clone info. Expression clone of mouse Lats2 cDNA.
Vector backbone pCMV/SV-Flag1 (Plasmid)
Size of vector backbone 4.6 kb
Gene/insert name Mouse Lats2 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Lats2 (NCBI Gene 50523) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12213 pCMV/SV-Flag-Lats2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12213_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12213_A6Aja.seq
>12213_12213_A6Aj_CMV-Forward_F01_16.ab1
    1 CTGTCCTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTCCACCAT GGACTACAAG GACGACGATG ACAAGGGATC
  181 CATGAGGCCA AAGACTTTTC CTGCCACAAC TTACTCTGGA AATAGCCGGC AGCGATTGCA
  241 AGAGATTCGA GAGGGGCTGA AGCAGCCATC CAAGGCTTCC ACCCAGGGGC TGCTGGTGGG
  301 ACCAAACAGT GACACTTCCC TGGATGCCAA AGTCCTGGGG AGCAAAGATG CCTCCAGGCA
  361 GCAGCAAATG AGAGCCACCC CGAAGTTTGG ACCTTATCAA AAAGCTCTCA GGGAAATCCG
  421 ATATTCCCTC CTGCCTTTTG CCAACGAGTC AGGCACTTCG GCAGCTGCAG AGGTGAACCG
  481 GCAGATGCTT CAGGAGTTGG TGAATGCGGG ATGTGACCAG GAGATGGCTG GCAGAGCGCT
  541 CAAGCAGACG GGCAGTAGGA GTATCGAAGC TGCCTTGGAG TACATCAGTA AGATGGGCTA
  601 CCTGGACCCC AGGAATGAGC AGATTGTGCG AGTCATCAAG CAGACCTCCC CAGGAAAGGG
  661 CCTGGCGCCC ACCCCGGTGA CTCGGCGGCC CAGTTTCGAG GGCACAGGGG AAGCACTCCC
  721 ATCCTACCAC CAGCTGGGTG GTGCAAACTA CGAGGGCCCC GCCGCACTGG AGGAGATGCC
  781 GCGGCAATAT TTAGACTTTC TCTTCCCTGG AGCCGGAGCC GGCACCCACG GTGCCCAGGC
  841 TCACCAGCAT CCTCCCAAAG GGTACAGCAC AGCAGTAGAG CCAAGTGCGC ACTTTCCGGG
  901 CACACACTAT GGTCGTGGTC ATCTACTATC GGAGCAGCCT GGGTATGGGG TGCAGCGCAG
  961 TTCCTCCCTT CCAGACAGAC GCCACCAGAT GCCTATTCCA GCATGACAGC CCACGTGGCC
 1021 CTCCGCAGCT CACTTCTGCC ATGCTGGCTG TACACTGCCT CGCACACAGT GCGCTACCCA
1081 CTGGCAGCAT ACATGTGTGG CACCGGGGGA TTCCCAACGT AT
//
Primer: GNP_primer#10 RDB12213_A6Ajb.seq
>12213_12213_A6Aj_pMAMneo_Reverse_D04_10.ab1
    1 TGTGCATGTC CACCACAGAA GTAAGGTTCC TTCACAAAGA TCCTCTAGCA TTTAGGTGAC
   61 ACTATAGAAT AGGGCCCTCG AGTTGTGGTT AACTGAGGCT TACACGTACA CCGGCTGGCA
  121 GCCCTCGGCC GCACCTTCCA AGTCCGCATC CCCTGGGTCT GCACTCTCTG CGGGCTCTGA
  181 GGGCTTCGGG CACCGGAAGG GATAGCCGTT GTCATCGAAG AACCTGCGGA AGGTGAACTC
  241 ATAGAAGGCG TGCTCTGGAT GCTTGCTGCT GGGGGAGGCC AGCGTGTCCC AGGCCTTGGC
  301 GCTCTCTCCG CTGGCCTCGT GCCAGGGGCT TTCTTCATCC ACCGGGTCAA AATTGGAGGT
  361 GTCCATGGGG TGGCTGATGG TGGGGACGTA GGGTGCAGGC TGCTTTCGGA TGTCACGGGA
  421 AAAGTCGATG GTGTTGAAGA ACGGGTGTGC CTTGAGGTCA TCTGCCCCAT CCCTGCCCAG
  481 GCGGCAGTCA GCCGCGCAGC ACAGCTTCGT GATGAGGTCT CGGGCCTCAG CGCTGAGCCT
  541 CACCTGCGTA GGGATATGCA GCGTGCTCTC CCAGTTGATC ACCTTCAGCT GCGTCTCTGT
  601 GGGGGTGGGG GCCAAGAAAG GCGGCTGCCC AACCAGCATC TCAAAGAGAA TCACACCGAC
  661 GCTCCACCAG TCACAGAGCT GCGTGTACCC TTTGCGGAGA AGCACCTCCG GAGCGATGTA
  721 ATTTGGTGTC CCGACAAGAG AATGTGCCAG GCACCTCTGG TGCTGCTTCT GCGCCCTCTG
  781 CTCCAGGGTC TTTAACCTGT CTCCACAGCG ACAGTTGGAA ACATCGTCCC AGAGGTCACC
  841 GGGCTCCATG CTGTCCTGTC TCATGTGGTT CCCTTTCTGG TAGTACTTGG AAATTGTGAG
  901 TCCACCTGAA ATCCAGTGCA GAGGCCAAAA TCTGTCAGCT TTAATATGAC CATCCAGGTC
  961 GATGAGTATG TTGTCAGCTT GATGTCCCGG TGGATAAAGC CCATCTTGTG ACACTTTCAT
 1021 GACAGGTCAC TTCTGCCAAT GTAGAGCGGT AGCTGTCTCA AGCAGACCTC ATTCTGATCA
1081 GCAAGGCTCA ATCATATTGC CGGA
//
Primer: Lats2#2 RDB12213_A6Ajc.seq
>12213_12213_A6Aj_Lats2#2_H01_22.ab1
    1 GACATCTAAG GAGACGATGT CCTGAACCGG AATCAAGTGG CCCATGTCAA GGCTGAGAGG
   61 GACATCCTGG CTGAAGCAGA CAATGAGTGG GTGGTCAAAC TCTACTACTC CTTCCAGGAC
  121 AAGGACAGCC TGTACTTTGT GATGGACTAC ATACCAGGCG GGGATATGAT GAGCCTGCTG
  181 ATCAGGATGG AGGTCTTCCC TGAGCACCTG GCCCGCTTCT ACATTGCAGA GTTGACCCTG
  241 GCCATTGAAA GTGTCCACAA GATGGGCTTT ATCCACCGGG ACATCAAGCC TGACAACATA
  301 CTCATCGACC TGGATGGTCA TATTAAGCTG ACAGATTTTG GCCTCTGCAC TGGATTCAGG
  361 TGGACTCACA ATTCCAAGTA CTACCAGAAA GGGAACCACA TGAGACAGGA CAGCATGGAG
  421 CCCGGTGACC TCTGGGACGA TGTTTCCAAC TGTCGCTGTG GAGACAGGTT AAAGACCCTG
  481 GAGCAGAGGG CGCAGAAGCA GCACCAGAGG TGCCTGGCAC ATTCTCTTGT CGGGACACCA
  541 AATTACATCG CTCCGGAGGT GCTTCTCCGC AAAGGGTACA CGCAGCTCTG TGACTGGTGG
  601 AGCGTCGGTG TGATTCTCTT TGAGATGCTG GTTGGGCAGC CGCCTTTCTT GGCCCCCACC
  661 CCCACAGAGA CGCAGCTGAA GGTGATCAAC TGGGAGAGCA CGCTGCATAT CCCTACGCAG
  721 GTGAGGCTCA GCGCTGAGGC CCGAGACCTC ATCACGAAGC TGTGCTGCGC GGCTGACTGC
  781 CGCCTGGGCA GGGATGGGGC AGATGACCTC AAGGCACACC CGTTCTTCAA CACCATCGAC
  841 TTTTCCCGTG ACATCCGAAA GCAGCCTGCA CCCTACGTCC CCACCATCAG CCACCCCATG
  901 GACACCTCCA ATTTTGACCC GGTGGATGAA GAAAGCCCCT GGCACGAGGC CAGCGGAGAG
  961 AGCGCCAAAG GCCTGGGACA CGCTGGGCCT CCCCCAGCAG CAAGCATCCA GAGCACGCCT
 1021 TCTATGAGTT CACCTTTCGC AGTTCTCGAT GACACGGCTA TCCCTTCGGT GCCGAGGCCC
 1081 TCAGAGCCCC GCAGAGAGTG CAGACCAAGG AATGCGAACT TGAAGATCGC GAGGTGCCAG
1141 CCGAGGGTA
//
Primer: RDB12213_A6Ajd.seq
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl