Resource data sheet
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pcDNA3.1-His-hGLI3

Expression vector of human Gli3 cDNA

Catalog number RDB08066
Resource name pcDNA3.1-His-hGLI3
Clone info. Expression vector of human Gli3 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Selectable markers Amp^r
Gene/insert name Human GLI3 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank human GLI3 (NCBI Gene 2737) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08066 pcDNA3.1-His-hGLI3 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08066_A7Ktp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB08066_A7Kta.seq
>08066_08066_A7Kt_CMV-Forward_D09_12_ABI24.ab1
    1 CGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTACCATG GGGGGTTCTC
  181 ATCATCATCA TCATCATGGT ATGGCTAGCA TGACTGGTGG ACAGCAAATG GGTCGGGATC
  241 TGTACGACGA TGACGATAAG GTACCTAAGG ATCCAGTGTG GTGGAATTCT GCAGATGAAG
  301 ACATCATGGA GGCCCAGTCC CACAGCTCCA CGACCACTGA AAAGAAAAAA GTTGAGAATT
  361 CCATAGTGAA GTGCTCCACT CGAACAGATG TGAGCGAGAA AGCCGTTGCC TCCAGCACCA
  421 CTTCTAATGA GGATGAAAGT CCTGGACAGA CTTATCACAG AGAGAGAAGA AACGCAATCA
  481 CTATGCAGCC ACAGAATGTC CAGGGGCTCA GCAAAGTCAG TGAGGAACCT TCAACATCGA
  541 GTGACGAGAG GGCCTCATTG ATCAAGAAAG AGATCCATGG GTCCCTGCCA CACGTGGCGG
  601 AGCCCTCTGT GCCGTACCGC GGGACGGTGT TTGCCATGGA CCCCAGGAAT GGTTACATGG
  661 AGCCCCACTA CCACCCTCCT CATCTTTTCC CTGCCTTCCA TCCTCCTGTA CCAATTGATG
  721 CCAGACATCA TGAGGGCCGT TACCATTACG ATCCATCTCC GATTCCTCCA TTGCATATGA
  781 CTTCCGCCTT ATCTAGTAGC CCTACGTATC CGGACCTGCC CTTCATTAGG ATCTCCCCAC
  841 ACCGGAACCC CACTGCTGCT TCCGAGTCTC CCTTCAGCCC TCCACATCCC TACATTAATC
  901 CCTACATGGA CTATATCCGC TCCTTGCACA GCAGCCCATC GCTCTCCATG ATCTCAGCAA
  961 CCCGTGGGCT GAGCCCTACA GATGCGCCCC CATGCAGGAG TCAGCCCAGC AGAATACTAT
 1021 CATTCAGATG GACCTGCTAA CTTGCTAAGC GGCAGGCCCC TATGCAGACA TAATACCCTT
1081 CAGCCTGGAC CAACCGGACG GCACGGGGGC CAATC
//
Primer: BGH_rev2 RDB08066_A7Ktb.seq
>08066_08066_A7Kt_BGH_rev2_E09_15_ABI24.ab1
    1 CTTTAGAAGG TCAGGGAGGG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA
   61 GGCACAGTCG AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGGCCGCTCA
  121 ACTCCTATTG ATTTCCGTTG GTTGCAGTCT TTTTTTCCTA AAGCCTATTG CATAACTGCA
  181 AGGAATTTGC TTTCTTCCGC TAGGGAGGTC AGCAAAGAAC TCATGTCCCC GATAGCCATG
  241 TTGGTGGTGC TCATGGACAG CGCTGGGAAT GGGAGGGACG CCCGAGGCGT GGTGAGGCGG
  301 GAGGAGCTAT GGGAAAGGTT CTGAATGATA CTTGGGCTCA GGGCCCCCGA CATCAGGCTG
  361 GAGTGGTCCC CATCGTCTAT GATGGCATCG AAGTCAATCT GTACCCCTTC CAGGTCATGG
  421 CTGTCGAGGC TGTCCACTGT GCTTGTCACC TGATTAGCAC CTGGGGAAAG TAACTCAGAG
  481 TTTTTGGCGC TGGTCCCCTG TAGCAGGCAG CTGGCGTCTG AAATAGAGAA TGAACCAGCT
  541 TTCGTGTCTT GCTGACTGAA GCCCACGGTT TGGTCATAGA ACTGACCAGA GTAATTCTGC
  601 AGATTAGAGC ACAGCGGATG GGGCTGCCCT TTCATCTCCA TCTTGATACC ATTCACCCTG
  661 CAGGTCTGAC TTGTGTCACT GAGCTGTCCT GACTGCAGAG CAAGGCTGTC CCTCGGCATA
  721 GCCTGGCGCC TGCTGCCCCC AAAGCTGGCA CATGGCTGGT AGCCCCTGAC AACTGCCAAG
  781 CTTGACGGCT GGCTGCCCAT GCCGTGAGCC CCTGGCAGGC AGCTCTCTGG CCCTTGGTAG
  841 ATGTTGATGT GTGAGGTAGC ACTAATCTGC CCAAGCATCT GCTGACCGGG GCGGCCTGCC
  901 CCCGGGTGCT GCATGCTGTC GCCGAGGAGC TGGTGAGCCA GGTACCCCTG TCCCACTGGG
  961 GTCCTGGTTT CTGCATGCCA TTCACCATGC TGCCAGCTGA CTCATTTGGC GCTACCGGCA
 1021 GGCCGAAATT CAGCTGGGCC CCCGCTCCCT TTGCATGGGG GTGCCTCCTT CCAGCTTTGA
 1081 GGCTTGGATT CCCGGCACTA CAGGCACCGT TCGAGTTGCA CCAAGGTCAC TGGCTGACCA
1141 TGAATGTGTC GAC
//
Primer: SV40pro_F RDB08066_A7Ktc.seq
>08066_08066_A7Kt_SV40pro_F_F09_18_ABI24.ab1
    1 CGAGGACTTT TTTGGGAGGC TAGGGCTTTT GCAAAAGCTC CCGGGAGCTT GTATATCCAT
   61 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
  121 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
  181 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
  241 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT
  301 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
  361 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
  421 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
  481 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
  541 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
  601 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC
  661 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT
  721 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
  781 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC
  841 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG
  901 ACTCTGGGGT TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA
  961 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTTCCGG GACGCCCGGC
 1021 TGGATGAATC TCAGCGCGGG GAATCTCATG CCTGGAGTTC CTCCGCCAAC CCCAACCTGT
1081 TATGGCCAGC TATATGGTTA CAATAAAGCC ATTTGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl