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pcDNA3.1-His-hGLI3

Expression vector of human Gli3 cDNA

Catalog number RDB08066
Resource name pcDNA3.1-His-hGLI3
Clone info. Expression vector of human Gli3 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Selectable markers Amp^r
Gene/insert name Human GLI3 cDNA
Depositor|Developer Sasaki, Hiroshi |
 
Other clones in our bank human GLI3 (NCBI Gene 2737) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08066 pcDNA3.1-His-hGLI3 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18391_B0I7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro,6X His,T7 tag,Xpress tag,insert 5'
Sequence file: RDB18391_B0I7a.seq check
>08066_18391_B0I7_3_CMV_Forward_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGCG GTANGCGTGT ACGGNGNGNA GGTCTATATA
   61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
  121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACC ATGGGGGGTT
  181 CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGG
  241 ATCTGTACGA CGATGACGAT AAGGTACCTA AGGATCCAGT GTGGTGGAAT TCTGCAGATG
  301 AAGACATCAT GGAGGCCCAG TCCCACAGCT CCACGACCAC TGAAAAGAAA AAAGTTGAGA
  361 ATTCCATAGT GAAGTGCTCC ACTCGAACAG ATGTGAGCGA GAAAGCCGTT GCCTCCAGCA
  421 CCACTTCTAA TGAGGATGAA AGTCCTGGAC AGACTTATCA CAGAGAGAGA AGAAACGCAA
  481 TCACTATGCA GCCACAGAAT GTCCAGGGGC TCAGCAAAGT CAGTGAGGAA CCTTCAACAT
  541 CGAGTGACGA GAGGGCCTCA TTGATCAAGA AAGAGATCCA TGGGTCCCTG CCACACGTGG
  601 CGGANCCCTC TGTGCCGTAC CGCGGGACGG TGTTTGCCAT GGACCCCAGG AATGGTTACA
  661 TGGAGCCCCA CTACCACCCT CCTCATCTTT TCCCTGCCTT CCATCCTCCT GTACCAATTG
  721 ATGCCAGACA TCATGANGGC CGTTACCATT ACGATCCATC TCCGATTCCT CCATTGCATA
  781 TGACTTCCGC CTTATCTAGT ANCCCTACGT ATCCGGACCT GCCCTTCATT ANGATCTCCC
  841 CACACCGGAN NCCNACNGCN GCTTCCNANN CNCCCTTCAG CCCTNCNCAT NCCNACATNA
901 ATCCNTACAT GGNNNATATN NNNNNCNNNC ACANCAGCCN NTCGNNNTNN NNNNNNNN
//
Primer: bGH_rev3
Region: bGHpA,insert 3'
Sequence file: RDB18391_B0I7b.seq check
>08066_18391_B0I7_3_BGH_rev3_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNGGNNNNN NACAGNNGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCT CAACTCCTAT
  121 TGATTTCCGT TGGTTGCAGT CTTTTTTTCC TAAAGCCTAT TGCATAACTG CAAGGAATTT
  181 GCTTTCTTCC GCTAGGGAGG TCAGCAAAGA ACTCATGTCC CCGATAGCCA TGTTGGTGGT
  241 GCTCATGGAC AGCGCTGGGA ATGGGAGGGA CGCCCGAGGC GTGGTGAGGC GGGAGGAGCT
  301 ATGGGAAAGG TTCTGAATGA TACTTGGGCT CAGGGCCCCC GACATCAGGC TGGAGTGGTC
  361 CCCATCGTCT ATGATGGCAT CGAAGTCAAT CTGTACCCCT TCCAGGTCAT GGCTGTCGAG
  421 GCTGTCCACT GTGCTTGTCA CCTGATTAGC ACCTGGGGAA AGTAACTCAG AGTTTTTGGC
  481 GCTGGTCCCC TGTAGCAGGC AGCTGGCGTC TGAAATAGAG AATGAACCAG CTTTCGTGTC
  541 TTGCTGACTG AAGCCCACGG TTTGGTCATA GAACTGACCA GAGTAATTCT GCAGATTAGA
  601 GCACAGCGGA TGGGGCTGCC CTTTCATCTC CATCTTGATA CCATTCACCC TGCAGGTCTG
  661 ACTTGTGTCA CTGAGCTGTC CTGACTGCAG AGCAAGGCTG TCCCTCGGCA TAGCCTGGCG
  721 CCTGCTGCCC CCAAAGCTGG CACATGGCTG GTAGCCCCTG ACAACTGCCA AGCTTGACGG
  781 CTGGCTGCNC ATGCCGTGAN CCNNNGGCAN GCANCTCTCT GGCCCTTGGT ANATGTTGAT
  841 GTGTGANGTA NCACTAATCT GNCNAAGCAT CNGCTNANCG GGGGCNGNCN NNCCCNGGNN
901 NCNGCNNGCT NNNNNNNANN NNNTGGNNAN CNNNNNNNCC NNNNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori,NeoR
Sequence file: RDB18391_B0I7c.seq check
>08066_18391_B0I7_3_SV40pro_ori_F_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNCCTCT GCCTCTGNNC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG
   61 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC
  121 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC
  181 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
  241 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
  301 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
  361 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
  421 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
  481 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
  541 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
  601 TTGTCGATCA GGATGATCTG GACGAANANC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
  661 CCAGGCTCAA GGCGCGCATG CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT
  721 GCTTGCCGAA TATCATGGTG GAANATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
  781 TGGGNGTGGC GGNNCGCTAT CANGNNNTAN CGTTGGCTAC CCGNGNTATT GCTGAAGANC
  841 TTGGCNGCGA ANNGGCNNNN CGCTTNNNCN NGCTTTACNG NATCGNNGCN NCNNNNTNNN
901 ANNNNATCGN CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
user report Patel, R., Novel GLI3 pathogenic variants in complex pre- and postaxial polysyndactyly and Greig cephalopolysyndactyly syndrome Am. J. Med. Genet. A 185 (1): 97-104 (2021). PMID 33058447.

2020.12.30

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