Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-pA83-Tead4

Expression clone of mouse Tead4

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13965_A6Bmp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,T7 prox2,6XHis,T7 tag,Xpress tag,insert 5' Sequence file: RDB13965_A6Bma.seq
>D03261A1_A6Bm_1_CMV-Forward_A01_01.ab1     1 ACGATCACTT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT   121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGTTAAGCT TGGTACCGAG CTCGGATCCA   181 CTAGTCCAGT GTGGTGGAAT TGTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC   241 GTTTAAACTT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG   301 CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGTACCAGG   361 ATCCAGTGTG GTGGAATTCA TTACCTCCAA CGAGTGGAGC TCTCCCGACT CCCCCGAGGG   421 GAGCAGCATC TCTGGGGGCA GCCAGGCACT GGACAAGCCC ATCGACAATG ATGCAGAGGG   481 TGTATGGAGC CCCGAAATTG AGCGAAGCTT CCAGGAGGCC CTGGCCATCT ACCCACCCTG   541 CGGCCGCCGC AAAATTATCC TGACGGAGGA AGGCAAGATG TATGGTCGGA ATGAGCTGAT   601 CGCACGCCAT ATCAAGCTCA GGACAGGGAA GACGCGCACA AGGAAGCAGG TCTCCAGCCA   661 CATCCAGGTG CTTGCCCGTC GAAAAGCCCG GGAGATCCAG GCCAAACTCA AGGACCAGGC   721 AGCTAAGAAC AAGGCCCTGC AGAGCATGGC TGCCATGTCG TCCGCACAGA TCGTCTCGGC   781 CACAGCCTTC CACAGTAAAA TGGCTCTTGC CCGGGGCCCT GGCTACCCAG CAATCTCAGG   841 GTTTTGGCAA GGAGCTTTGC CAGGCCAACC TGGAACATCC CACGATGTGA AAACCTTTCT   901 CTCAAAACAC CTACCCTGTC CAGCCTCCGC TGCCTCTGCC AGGCTTTGAG TCTCCTGCAG   961 GGCCCACCCC TCACCCTCCG CACGGCTCGC TCCTCCGTGG CAAGGCCGCA GCATAGCTAG  1021 CTCAGCTCTG GATGGTGGAG TCTCGCTTTC TGAGCGCAGC AGATCGGACA CTACCACAAC  1081 ACCTGTTTGG TGCCAATCAG CCAGTCAGCC AGCCTACAGT GAACCCTTAC CCTCGGAACC 1141 TGTGGTGG //
Primer: GNP Primer #10 Region: insert 3' Sequence file: RDB13965_A6Bmb.seq
>D03261A1_A6Bm_1_GNPprimer#10_B01_04.ab1     1 TTTTTTTGGT GCCATTCATT TAAAAAGATA ACGTTTACAG GTTCTTTGGT GCCCATAGTC    61 GAGCGGCCGC CACTGTGCTG GATATCTGCA GAATTTAGAA GGCACAGTCG AGGCTGATCA   121 GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CTATTCTTTC ACAAGTCGGT AGATGTGGTG   181 CTGAGCCCCA TGCTCGCTGG CTGAAACTTC GAAGACATAT GCAATACACA GCAAGGTCTC   241 CTGTGTGTCT CGGTTGGTGA CCACCTGCAG GATGGTGAAG TTCTCCAGCA CACTGTTCAT   301 CATGTACTTC TCGGGTAAGT GTTTCAGCTT GTGGATAAAG TTGATCATGT ACTCACAGAG   361 AGGGGACCGG TGGATGCGGT ACAAATAGTG GCCATTCTCA TAGCGGGCAT ACTCCGTCTC   421 AACTTTCTCC ACCACTTGCT TGCCAAATGA GCAGACCTTC GTAGAGCAGG TGATAATCAT   481 GTTCTCCGGG CTCTCGTACT GGCTGGAGAC CCCATAGAAG GCGCTGCCCT CGTCATCGAT   541 GTTGGTATTG AGGTCTGCCC AGAACTTCAC AAGGAAGAAG GCATTAGAGG GCCCCCGTTC   601 AAATAGCTCC TTGAGGCCTC CCTTCTTCTC TGGGAACTTG TCATAGATTT GGCGGATGTC   661 CACAGTTTCG AGGTAGGGGT CACTGTAGCT TGGGCTTGAC TGGCTGATGT GCACAAACAG   721 GTGTTTGTTG TATGTGTCCG GATCTTGCTG GCGCTCCAGG AAAGCCGAGA ACTCCAACAT   781 CCAGAGCTTG GAGCTAGCTA TGCTGCGGCC TTGCCACGGA GGAGCGAGCG GTGCGGAGGG   841 TGAGGGGTGG GCCCTGCAGG AGACTCAAAG CCTGGCAGAG GCAGCGGAGG CTGGACAGGG   901 TAGGTGTTTT GAGAGAAAGG TTTCACATCG TGGGATGTTC CAGGTTGGCC TGGCAAGCTC   961 CTTGCCAAAC CCTGAGATTG CTGGTAGCCA GGGCCCGGCA GAGCCATTTA CTGTGGATCT  1021 GTGGCGAGAA CGATCTGGTG CGGGACGACA TGCCAGGCAT GCTCTGCAGG GCTTGTTCCT  1081 AAGCTGCTGG TCTGGAGTTG TCCTGGATCC CGCTTCGGAC GGGCAGCACC TGGAATGTGG 1141 CTGGGGAACT GCT //
Primer: BGH_rev2 Region: bGHpA,6xHis,Myc Sequence file: RDB13965_A6Bmc.seq
>D03261A1_A6Bm_1_BGH_rev2_C01_07.ab1     1 CGATAGGTCA GATGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC GGTATGCATA   121 TTCAGATCCT CTTCTGAGAT GAGTTTTTGT TCGAAGGGCC CTCTAGACTC GAGGTACGCC   181 ACAGCGTATG ATGCGTATTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT   241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT CTTTCCCAAA CTTTTTTTTG GGCCCATTCA   301 ATTTAAAAAA AAAACTTTTA CGGGTTTTTT GGGCCCCAAA ACCAACCGCC CCCCCCGGGG   361 GGGGAATTCT CCAAAATTTA AAAGGCCCAG CCGGGGGTTT TCCGGGGGTT TAAAGGGGCC   421 CCCAAAACCC AAGCTTTTTT TTCAAAAATG GGAAAAGGGG GGGGGAACCC CCCTGCCCCC   481 GGGGGAAAAT TTCAAAAACA TTTGCAAACC CACCCAGGGT CCCTTGGGTT TCCCGGTTGG   541 GGACCCCCCG GGGGGGGGGA AATTTCTCCC CCCACCTTTT CATCTTTTCT TTTCGGGGAA   601 AATGTTTCCC CTTTGGGAAA AAGTTTTTTT TTCCCCCCAA AAGGGGGCCC CGGGAATCCG   661 GAAAAAAAAG GGGCCTTTTT CAAAGGGGGG AAACCCCGTC CAAATTTTCC CCCCCCTTTT   721 TTCAAAAAAG ACAAACCTTT TAAAAAAGGG GAAAATCTTT TTCCCGGGGC TCCCTCCGGG   781 GGGGAACCCC CAAAAAAGGG CCCCCCCCCC CCCAAGTTTG TTTGGGGCTC CCCCCAACTT   841 CCCAAGAAAA GGGTTTTAGG GCCCCCTATA AAACCCCTAG GGCCTCTCTT TTCTTCGGGG   901 ATTTGTCAAT ATATTGCGGG AAGTCCCCTT TTAGAGAGAG GGCACCTGTA CCTTTGCGCT   961 ACACAGATGA GAGACCCACA CCAGGTGTTG TTGTGTGTTT CGTATTCTGT GGCGCTCCAG  1021 AAGACGAAAA TCCTACACCC AGCTGTGAGA TACATTTCGC GCGCCTTACA AAGAGAGACA 1081 CGTGAAGTAG AGTGTGCTCT GGAATACTAC GTTCGGGAAG AGCAGCG //
Primer: SV40pro_F Region: SV40 pro_ori,NeoR Sequence file: RDB13965_A6Bmd.seq
>D03261A1_A6Bm_1_SV40pro_F_D01_10.ab1     1 CCTTGGGCTG TAGCAATCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC   121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT   661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCCAAGCG   961 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AGGTGGGCTT  1021 CGGATCGTTT CCGGACGCCG GCTGGAATGA TCCTCCAGCG CGGGGGATCT CATGCCTGAA  1081 GTCCTCGCCC ACCCCGACTG TTATGCAGCT ATATGGCTAC AATAAGCCAT TAGCCATCCA 1141 CGAATTCACC A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl