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pcDNA3.1-pA83-Tead4 (#RDB12185)

Expression clone of mouse Tead4

Clone info. Expression clone of mouse Tead4.
Comment See Fig. 2B of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Known differences (at least): C to G substitution (Pro, synonym) at 1265 nt of NM_011567.2.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead4
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12185 pcDNA3.1-pA83-Tead4 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-Tead4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12185).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13965_A6Bmp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,T7 prox2,6XHis,T7 tag,Xpress tag,insert 5'
Sequence file: RDB13965_A6Bma.seq check
>D03261A1_A6Bm_1_CMV-Forward_A01_01.ab1
    1 ACGATCACTT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGTTAAGCT TGGTACCGAG CTCGGATCCA
  181 CTAGTCCAGT GTGGTGGAAT TGTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC
  241 GTTTAAACTT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG
  301 CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGTACCAGG
  361 ATCCAGTGTG GTGGAATTCA TTACCTCCAA CGAGTGGAGC TCTCCCGACT CCCCCGAGGG
  421 GAGCAGCATC TCTGGGGGCA GCCAGGCACT GGACAAGCCC ATCGACAATG ATGCAGAGGG
  481 TGTATGGAGC CCCGAAATTG AGCGAAGCTT CCAGGAGGCC CTGGCCATCT ACCCACCCTG
  541 CGGCCGCCGC AAAATTATCC TGACGGAGGA AGGCAAGATG TATGGTCGGA ATGAGCTGAT
  601 CGCACGCCAT ATCAAGCTCA GGACAGGGAA GACGCGCACA AGGAAGCAGG TCTCCAGCCA
  661 CATCCAGGTG CTTGCCCGTC GAAAAGCCCG GGAGATCCAG GCCAAACTCA AGGACCAGGC
  721 AGCTAAGAAC AAGGCCCTGC AGAGCATGGC TGCCATGTCG TCCGCACAGA TCGTCTCGGC
  781 CACAGCCTTC CACAGTAAAA TGGCTCTTGC CCGGGGCCCT GGCTACCCAG CAATCTCAGG
  841 GTTTTGGCAA GGAGCTTTGC CAGGCCAACC TGGAACATCC CACGATGTGA AAACCTTTCT
  901 CTCAAAACAC CTACCCTGTC CAGCCTCCGC TGCCTCTGCC AGGCTTTGAG TCTCCTGCAG
  961 GGCCCACCCC TCACCCTCCG CACGGCTCGC TCCTCCGTGG CAAGGCCGCA GCATAGCTAG
 1021 CTCAGCTCTG GATGGTGGAG TCTCGCTTTC TGAGCGCAGC AGATCGGACA CTACCACAAC
 1081 ACCTGTTTGG TGCCAATCAG CCAGTCAGCC AGCCTACAGT GAACCCTTAC CCTCGGAACC
1141 TGTGGTGG
//
Primer: GNP Primer #10 (Pr0763)
Region: insert 3'
Sequence file: RDB13965_A6Bmb.seq check
>D03261A1_A6Bm_1_GNPprimer#10_B01_04.ab1
    1 TTTTTTTGGT GCCATTCATT TAAAAAGATA ACGTTTACAG GTTCTTTGGT GCCCATAGTC
   61 GAGCGGCCGC CACTGTGCTG GATATCTGCA GAATTTAGAA GGCACAGTCG AGGCTGATCA
  121 GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CTATTCTTTC ACAAGTCGGT AGATGTGGTG
  181 CTGAGCCCCA TGCTCGCTGG CTGAAACTTC GAAGACATAT GCAATACACA GCAAGGTCTC
  241 CTGTGTGTCT CGGTTGGTGA CCACCTGCAG GATGGTGAAG TTCTCCAGCA CACTGTTCAT
  301 CATGTACTTC TCGGGTAAGT GTTTCAGCTT GTGGATAAAG TTGATCATGT ACTCACAGAG
  361 AGGGGACCGG TGGATGCGGT ACAAATAGTG GCCATTCTCA TAGCGGGCAT ACTCCGTCTC
  421 AACTTTCTCC ACCACTTGCT TGCCAAATGA GCAGACCTTC GTAGAGCAGG TGATAATCAT
  481 GTTCTCCGGG CTCTCGTACT GGCTGGAGAC CCCATAGAAG GCGCTGCCCT CGTCATCGAT
  541 GTTGGTATTG AGGTCTGCCC AGAACTTCAC AAGGAAGAAG GCATTAGAGG GCCCCCGTTC
  601 AAATAGCTCC TTGAGGCCTC CCTTCTTCTC TGGGAACTTG TCATAGATTT GGCGGATGTC
  661 CACAGTTTCG AGGTAGGGGT CACTGTAGCT TGGGCTTGAC TGGCTGATGT GCACAAACAG
  721 GTGTTTGTTG TATGTGTCCG GATCTTGCTG GCGCTCCAGG AAAGCCGAGA ACTCCAACAT
  781 CCAGAGCTTG GAGCTAGCTA TGCTGCGGCC TTGCCACGGA GGAGCGAGCG GTGCGGAGGG
  841 TGAGGGGTGG GCCCTGCAGG AGACTCAAAG CCTGGCAGAG GCAGCGGAGG CTGGACAGGG
  901 TAGGTGTTTT GAGAGAAAGG TTTCACATCG TGGGATGTTC CAGGTTGGCC TGGCAAGCTC
  961 CTTGCCAAAC CCTGAGATTG CTGGTAGCCA GGGCCCGGCA GAGCCATTTA CTGTGGATCT
 1021 GTGGCGAGAA CGATCTGGTG CGGGACGACA TGCCAGGCAT GCTCTGCAGG GCTTGTTCCT
 1081 AAGCTGCTGG TCTGGAGTTG TCCTGGATCC CGCTTCGGAC GGGCAGCACC TGGAATGTGG
1141 CTGGGGAACT GCT
//
Primer: BGH_rev2 (Pr0606)
Region: bGHpA,6xHis,Myc
Sequence file: RDB13965_A6Bmc.seq check
>D03261A1_A6Bm_1_BGH_rev2_C01_07.ab1
    1 CGATAGGTCA GATGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA
   61 GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC GGTATGCATA
  121 TTCAGATCCT CTTCTGAGAT GAGTTTTTGT TCGAAGGGCC CTCTAGACTC GAGGTACGCC
  181 ACAGCGTATG ATGCGTATTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT CTTTCCCAAA CTTTTTTTTG GGCCCATTCA
  301 ATTTAAAAAA AAAACTTTTA CGGGTTTTTT GGGCCCCAAA ACCAACCGCC CCCCCCGGGG
  361 GGGGAATTCT CCAAAATTTA AAAGGCCCAG CCGGGGGTTT TCCGGGGGTT TAAAGGGGCC
  421 CCCAAAACCC AAGCTTTTTT TTCAAAAATG GGAAAAGGGG GGGGGAACCC CCCTGCCCCC
  481 GGGGGAAAAT TTCAAAAACA TTTGCAAACC CACCCAGGGT CCCTTGGGTT TCCCGGTTGG
  541 GGACCCCCCG GGGGGGGGGA AATTTCTCCC CCCACCTTTT CATCTTTTCT TTTCGGGGAA
  601 AATGTTTCCC CTTTGGGAAA AAGTTTTTTT TTCCCCCCAA AAGGGGGCCC CGGGAATCCG
  661 GAAAAAAAAG GGGCCTTTTT CAAAGGGGGG AAACCCCGTC CAAATTTTCC CCCCCCTTTT
  721 TTCAAAAAAG ACAAACCTTT TAAAAAAGGG GAAAATCTTT TTCCCGGGGC TCCCTCCGGG
  781 GGGGAACCCC CAAAAAAGGG CCCCCCCCCC CCCAAGTTTG TTTGGGGCTC CCCCCAACTT
  841 CCCAAGAAAA GGGTTTTAGG GCCCCCTATA AAACCCCTAG GGCCTCTCTT TTCTTCGGGG
  901 ATTTGTCAAT ATATTGCGGG AAGTCCCCTT TTAGAGAGAG GGCACCTGTA CCTTTGCGCT
  961 ACACAGATGA GAGACCCACA CCAGGTGTTG TTGTGTGTTT CGTATTCTGT GGCGCTCCAG
 1021 AAGACGAAAA TCCTACACCC AGCTGTGAGA TACATTTCGC GCGCCTTACA AAGAGAGACA
1081 CGTGAAGTAG AGTGTGCTCT GGAATACTAC GTTCGGGAAG AGCAGCG
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB13965_A6Bmd.seq check
>D03261A1_A6Bm_1_SV40pro_F_D01_10.ab1
    1 CCTTGGGCTG TAGCAATCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCCAAGCG
  961 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AGGTGGGCTT
 1021 CGGATCGTTT CCGGACGCCG GCTGGAATGA TCCTCCAGCG CGGGGGATCT CATGCCTGAA
 1081 GTCCTCGCCC ACCCCGACTG TTATGCAGCT ATATGGCTAC AATAAGCCAT TAGCCATCCA
1141 CGAATTCACC A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]