Resource data sheet
Prev.

HA-Amot130/pcDNA3.1-pA83

Expression clone of mouse Amot (p130)

Catalog number RDB12203
Resource name HA-Amot130/pcDNA3.1-pA83
Clone info. Expression clone of mouse Amot (p130). HA-tag.
See Fig. 4A of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Amot (NCBI Gene 27494) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12203 HA-Amot130/pcDNA3.1-pA83 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12203_A5Hpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12203_A5Hpa.seq
>12203_12203_A5Hp_CMV-Forward_A04_01.ab1
    1 GTAGTCGTCA CTCGCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA GCTTGCCGCC ACCATGTACC
  181 CATACGACGT TCCAGATTAC GCTGGATCCA GAAGTTCTGA CGATCAGCCA AGTGGAGGAA
  241 CGACGGTACT GCAACGGCTG CTTCAAGAGC AGCTTCGCTA TGGCAATCCC AGTGAGAATC
  301 GCAGTCTGCT TGCCATACAC CAGCAAGCCA CAGGGAATAG CTCTCCATTT TCCACGGGCA
  361 GTGGGAACCA AGGCCCTCAG AATGATGTAT TGAGTTCCCA GGATCACCAC CAGCAGCAGC
  421 TTGTGGCACA CCCTGCTCGC CAAGAGCCCC AGGGTCAGGA AATCCAGTCA GAAAACGGCG
  481 TCATGGAGAA GCAGCTGTCC CCTCGAATGC AGAATAACGA AGAACTCCCA ACCTATGAAG
  541 AGGCCAAGGT CCAGTCCCAG TACTTCAGGG GCCAGCAGCA TGCTAGTGTT GGGGCTGCTT
  601 TCTATGTCAC AGGAGTCACC AACCAGAAGA TGAGAACCGA GGGACGCCCG TCAGTCCAGC
  661 GCCTCACGCC CGGGAAGATG CACCAAGATG AAGGCCTTCG AGATCTTAAG CAAGGGCATG
  721 TCCGATCCTT GAGTGAAAGA CTGATGCAGA TGTCATTGGC TACTAGTGGA GTTAAAGCCC
  781 ACCCACCTGT TACCAGTGCT CCTCTCTCCC CACCACAACC CAATGATCTC TACAAGAATG
  841 CCACCAGTTC CAGTGAATTC TACAAGGCCC AAGGGCCACC TCCAAGCCAA CATAGTCTGA
  901 AGGGCATGGA ACACCGAGGT CCCCCGCCAG AATACCCTTT CAAGGGCGTG CCATCCCAGT
  961 CTGTAGTGTG TAAGTCCCAA GAGCCAGGGC ACTTCTATAG TGAGCATCGC CCTGAACCAG
 1021 CCCAGGAGAA CAGAGGGGCA AACTGATGAG TATCAGCACC CCCCCTGAGT ATGGGGGCAG
 1081 CCAGGGCAAC ACAGACATCT CCTCCGCTGT CCTTTGTCAG CAGGACTCCT CAACCTCATA
1141 GGCCTTACTT CCTTCCTTGA CAGCTGGAGC ATCAAGGCTT GCGCTCTTGG TT
//
Primer: mAmot-M#1 RDB12203_A5Hpb.seq
>12203_12203_A5Hp_mAmot-M#1_E08_14.ab1
    1 AGGGGAGAGC TACTCAGCTA TGCCTCGGGC TCAGCAGTCT GCTTCTTATC AGCCCATGCC
   61 AGCTGACCCT TTTGCTATCG TCTCTAGAGC TCAGCAGATG GTTGAGATCC TGTCTGATGA
  121 GAATCGCAAC TTGCGGCAGG AGTTGGATGG ATGCTATGAG AAGGTGGCGA GACTGCAGAA
  181 GGTGGAGACA GAAATCCAAC GGGTCTCAGA GGCTTATGAG AACTTGGTGA AGTCATCTTC
  241 CAAAAGAGAG GCTCTGGAGA AAGCCATGAG GAACAAGCTG GAGGGCGAGA TTAGAAGGAT
  301 GCATGACTTC AACAGAGATC TGAGAGACCG TCTAGAGACT GCCAACAAGC AGCTGGCAGA
  361 GAAGGAGTAC GAGGGGTCCG AGGACACCAG GAAGACCATC TCGCAGCTCT TTGCCAAACA
  421 TAAAGAAAAC CAGCGGGAGA AGGAGAAGCT GGAAGCTGAG CTGGCTACTG CCCGTTCTAC
  481 CAATGAAGAC CAAAGGCGAC ACATTGAAAT CCGAGACCAG GCATTGAGCA ATGCCCAGGC
  541 GAAGGTGGTG AAGCTGGAAG AAGAGCTGAA AAAGAAGCAA GTGTACGTAG ATAAGGTGGA
  601 GAAGATGCAG CAGGCCCTTG TGCAGCTCCA GGCCGCCTGT GAGAAGCGTG AGCAGCTGGA
  661 ACACCGGCTC CGGACACGTT TGGAGAGGGA ACTGGAATCC CTTAGAATCC AGCAGCGCCA
  721 GGGCAACTCC CAGCCCACTA ATGCTTCAGA ATACAATGCC GCTGCACTGA TGGAGCTCCT
  781 CCGGGAGAAG GAAGAGAGAA TTCTGGCCCT GGAAGCAGAT ATGACCAAGT GGGAGCAAAA
  841 GTATTTGGAA GAGAATGTGA TGAGACACTT TGCTCTGGAT GCTGCTGCAA CTGTGGCTGC
  901 ACAGAGAGAC ACAACAGTCA TTAGCCACTC TCCTAACACC AGCTATGACA CAGCCCTTAG
  961 AGGCCCGCAT CCAGAAGGAA GAGGAGAAAT CCTGATGGGC CAACAAGCGT TGCTTGACAT
 1021 GGAAGGAGGA TTAAGACCTC ATGCCAGATA TGAAAAGATG CAATGATCAG TGCTGCAGCA
 1081 GCGCTCCAGG AAAGAAGCGA GTTAAACCAG AGCAACTTCG ATCCAATGCG CAGTCAGTCC
1141 TGAATGTTCC GATCTTTTTT CA
//
Primer: mAmot-M#2 RDB12203_A5Hpc.seq
>12203_12203_A5Hp_mAmot-M#2_H10_22.ab1
    1 TCGTTATGAG CAGACTGCTG AGCCGAGGCA TAGCTGAGTA GGCTTCTCCT GGCTGCTGCT
   61 GCGCTGAAGT CAGGCTGGCC TGGGCGTGAC GGTAGTGGTC CCCCTGATGA CTCTGACTCG
  121 AAAGAAGATG CTGCTGATGC CTTGACAGGT GAGCAGAGTG GTCACCTTGG TTCGGGACCA
  181 GGTGTTGACC AGGAGGCAAT CTAGTGGACG GCGGAGACTG CAGCAAGGGC AAGCTGGAAG
  241 CCCCAGCTGT CAGGGAAGAA GTAGGGCTAT GAGGTTGAGA GTTCCTGGCT GACAAAGACA
  301 GCGAGGAGAT GTCCTGTGTT GCCCTGGCTG CCCCATACTC AGGGGGGTGC TGATACCTCA
  361 TCAGTTGCCC CTCTGTTCTC CCTGGCTGGT TCAGGCGATG CTCACTATAG AAGTGCCCTG
  421 GCTCTTGGGA CTTACACACT ACAGACTGGG ATGGCACGCC CTTGAAAGGG TATTCTGGCG
  481 GGGGACCTCG GTGTTCCATG CCCTTCAGAC TATGTTGGCT TGGAGGTGGC CCTTGGGCCT
  541 TGTAGAATTC ACTGGAACTG GTGGCATTCT TGTAGAGATC ATTGGGTTGT GGTGGGGAGA
  601 GAGGAGCACT GGTAACAGGT GGGTGGGCTT TAACTCCACT AGTAGCCAAT GACATCTGCA
  661 TCAGTCTTTC ACTCAAGGAT CGGACATGCC CTTGCTTAAG ATCTCGAAGG CCTTCATCTT
  721 GGTGCATCTT CCCGGGCGTG AGGCGCTGGA CTGACGGGCG TCCCTCGGTT CTCATCTTCT
  781 GGTTGGTGAC TCCTGTGACA TAGAAAGCAG CCCCAACACT AGCATGCTGC TGGCCCCTGA
  841 AGTACTGGGA CTGGACCTTG GCCTCTTCAT AGGTTGGGAG TTCTTCGTTA TTCTGCATTC
  901 GAGGGGACAG CTGCTTCTCC ATGACGCCGT TTTCTGACTG GATTTCCTGA CCCTGGGGCT
  961 CTTGGCGAGC AGGGTGTGCC ACAAGCTGCT GCTGGTGGTG ATCCTGGGAA CTCAATACAT
 1021 CATTCTGAGG GCCTTGGTTC CCACTGCCCG TGGAAAATGG AGAGCTATTC CCTGGTGGCT
 1081 TGCTGGTGTA TGGGCAAGCA GACTGCGAAT CTCACTTGGA ATGGCATTAG CGGAGCTGCT
1141 CTTTGAAGGC AGCCCGTTGC CAGTTAACCC GGTG
//
Primer: GNP_primer#10 RDB12203_A5Hpd.seq
>12203_12203_A5Hp_GNP_primer#10_B04_04.ab1
    1 TTTATTTTGT GCAATTCATT TAAAAAGATA ACGTTTACAG GTTCTTTGGT GCCCATAGTC
   61 GAGCGGCCAG CTTTGAGCGT TCTGCTTAAC CTATCTTTTT GAGGTACTCC CTAAGTATTC
  121 CAAAATAGAT TGGAATTCGT TTCTATGGTA TTACTTATGT ACTACCAGAG TCCAATAAAA
  181 AGTCCCTTTA GAAAAATCAT CCCCACCTCC CCACCCTATC TTCACCCCAG ATTAAACAAA
  241 TGAAAGGGAA AAATTATTAA AAAGAACTCT TGCTGATAAC GCCAGCTCTT GATTGTGCTG
  301 TTTAGATGAG ATATTCCACC ATCTCTGCAT CAGGCTCTTG CCCCAGGATC TGAATGGGTG
  361 TCTTTCTTTC CAGAGTGCTG GAGTGGAAAG CAGGGGCATC TGGCTTATCT GGGTTGCACA
  421 TCAGATTTGG TGTAGACAAG CGGCGTGGTC CGGAACTGGC ACCTGGATTT GCAGGACCCT
  481 CAGTTTGAAC CAAAGCTGGA GTTGGAGTTG GAACTGGACT AGGAGGTTCA GTCGGAGCTG
  541 GAGTTGATGC CTGAGTCGGA GTCGGAGCAG AAGCCTGAGC CGTTGCTGGA GCCGGAATCG
  601 AAGCTGGAGA TGGAACCGGA GCCGAAGTAG CTGGAGCAAC CTGAATAGCA GTAGTGGCAG
  661 CAGCAGCAGC AGCAGTGATA GCAGCAGTAG TAGCTGCAGC AACAGCAGCA GTAGCAGTGG
  721 CAGCAGTTGG AGAAACAGTA GCAGGAGTAA GAGATGCAGC AGCTGGAATC TGAACAGCAG
  781 CAGCTGGAGA GACAGGAGCA GCTGGAGGCA CTACAGCAGC TGGAGCGACA GGAGCAGCTG
  841 GAGAGATAGA ATTAGCTGCA GAGACAGAAG TAGCTGCAGA GACAGAAGTA GCTGGAGCGG
  901 CAGCAGCGGC AAGAGCAGCA GCGTTTGCTG GAGATGGGGT GGCGGCAGCA GCAGCTGGAG
  961 CAGCAACAGC AACTGGGAGC AGCAGCTACC ATGATAGTGG TGTTGGTGGC AGCAGTGGCA
 1021 GTGTTGGTGG CAGCAGTTGC ATTGATGGCA GCAGCAGGGG CAGCAGCAGC AACCTGGGAC
 1081 TAGCCATTGA GCCGGAGAAT GGGGAGTGAC AGCTGGCGGT TTGGGTCGGA TTTCGGAGCC
 1141 AGGTTCAGTT GGGTATGCTG CAATCCGCCA ACTGCCGTGT CTCATTGGAC CGTGAAGAGA
1201 AGGGGGGAGG AGTGAAGAAA TCCGAGTCGT
//
Primer: BGH_rev RDB12203_A5Hpe.seq
>12203_12203_A5Hp_BGHrev_G08_20.ab1
    1 CACTACTCAT GGTGATGGTG ATGATGACCG GTATGCATAT TCAGATCCTC TTCTGAGATG
   61 AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA CAGCGTATGA TGCGTATTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTTTTC CTTCCCCAAC TTTTTTTTGG GCCCCTTCCT TTTAAAAAAA AAACCTTTTC
  241 GGGGTTTTTG GGGCCCCAAA CCCAGGGGCC CCTTTTAAAA CCCCTTTCGC TTAACCCTTT
  301 TTTTTGGGGG CCCCCCCAAA ATTCCCAAAA AAAAAGGGAA ACCCTTTCCG GGGGTTTTCC
  361 TTGGGCCCCC CCAAACCCCA AAAAAACCCC CTTTAAAAAA AATCCCCCCC CCCCCCCCCC
  421 CCTTTTTCCC CCCCAATTAA ACAAAGGGAG GGGAAAAATT TTTAAAAAAA ACCCTTTGGA
  481 AAACCCCCCC CCTTGATTGG GGGTTTTAAA AAAAAATTCC CCCTTCCCGG AAAAGGGTTT
  541 TGCCCCAAAA TTTGGAGGGG GGTTTCCTTT CAAAAGGGGG GGGGGGAACC GGGGGCTTCC
  601 GTTATACCTG TTTTGCCTAC ATATGGTGAA ACACCGCGGT CCGGGAGGGG CCCGGATTTG
  661 AGGACCTCGT TTGACCAAGC TGAATTGATT GAACGGACTA GAGTCCTCGA CTGATTATCC
  721 TGATCGATCG AACAACCGGG CTTGGGGGCC GGATCAGCTG GATGACCGGA CCAAGTTCGA
  781 CACTGGATCC CATGGCCACC CCCCCAGTTA GCCAATAACT CGCACAGCAG ATACTTGTCC
  841 GTGGAACTTC AAGGTTTAAT TTCCGCGTAT CGACGGCGTG ACGGACCTTG ACATGTGACG
901 ATCCTGTAAT ATTCGAAAAA
//
Primer: SV40pro_F RDB12203_A5Hpf.seq
>12203_12203_A5Hp_SV40pro_F_D04_10.ab1
    1 GCGCTTGGTC ATTAACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCAAT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
  121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
  661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGCGAAATG ACCGACCAAG
  961 CGACGCCCAA CCCTGCCATC ACGAGATTTC GATTCCCACC GCCGGCCTTC TATGAAAGGT
 1021 TGGGCGTCGG AAATCGTTTT CCGGGGACGC CGGGCTGGAA TGGATTCGTC CAGCGACGGG
1081 GGAATCTCAA TGGCGTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl