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pMYs-Tead1-VP16-IRES-EGFP (#RDB12172)

Retroviral vector of mouse Tead1, constitutive active

Clone info. Retroviral vector of mouse Tead1, activation domain of herpes simplex virus VP16, constitutive active.
Comment N-terminal FLAG and Myc-tag. Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.2 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead1 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infections virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12172 pMYs-Tead1-VP16-IRES-EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMYs-Tead1-VP16-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12172).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12172_A5Esp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro(PFB-5') ()
Sequence file: RDB12172_A5Esa.seq check
>12172_12172_A5Es_5'Retro(PFB-5')_C07_07.ab1
    1 CGGGTCGTTC GAATATTGCG CGGACTGCTA GTTATTAAGG ATCCCAGTGT GGTGGTACGG
   61 GAATTCCTGC AGGCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTAA ATTCCACCAT
  121 GGACTACAAG GACGACGATG ACAAGGGATC CGAATTCGAG CTCCGTCGAA TGGAACAGAA
  181 ACTGATTAGC GAAGAAGATC TGAACGAAGG CCGCATGGAG CCCAGCAGCT GGAGCGGCAG
  241 CGAGAGCCCT GCCGAAAACA TGGAAAGGAT GAGCGACTCG GCAGATAAGC CGATTGACAA
  301 CGACGCGGAG GGCGTCTGGA GTCCTGATAT TGAGCAGAGT TTCCAGGAGG CCCTGGCTAT
  361 CTATCCGCCG TGTGGGAGGA GAAAAATCAT CTTATCAGAC GAAGGCAAAA TGTATGGTAG
  421 AAATGAATTG ATAGCCAGAT ACATCAAACT CAGGACGGGA AAGACAAGGA CCAGGAAGCA
  481 GGTGTCTAGT CACATTCAGG TTCTTGCCAG AAGGAAATCT CGTGATTTTC ATTCCAAGCT
  541 GAAGGATCAG ACTGCCAAGG ACAAGGCCCT GCAGCACATG GCTGCCATGT CATCAGCCCA
  601 GATCGTCTCG GCTACTGCCA TCCACAACAA GCTGGGGCTG CCTGGGATTC CACGCCCCAC
  661 CTTCCCGGCC CCCCCGACCG ATGTCAGCCT GGGGGACGAG CTCCACTTAG ACGGCGAGGA
  721 CGTGGCGATG GCGCATGCCG ACGCGCTAGA CGATTTCGAT CTGGACATGT TGGGGGACGG
  781 GGATTCCCCG GGTCCGGGAT TTACCCCCCA CGACTCCGCC CCCTACGGCG CTCTGGATAT
  841 GGCCGACTTC GAGTTTGAGC AGATGTTTAC CGATGCCCTT GGAATTGACG AGTACGGTGG
  901 GTAGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG ACTATGGGCA CCAAAGAACC
  961 CTGTAAACGT TATCTTTTTA AATTGATGTG CACAAATAAA GTTGGAAGAA AATCACATCA
 1021 AATATACAAA ACATATAGAC ATAAAGACAT AAAATAAAAT TCCGCCACTT CATTACGCGC
 1081 TGTGTGGGCG TATCCTTCGA GTCTTAGAGA GTCTCGAGGC TGGCGCGGCC CGGACGTACG
1141 TAGCTCGTCC TCTTATCCAT CT
//
Primer: IRES-F ()
Sequence file: RDB12172_A5Esb.seq check
>12172_12172_A5Es_IRES-F_D07_10.ab1
    1 GGATCTTATG TGTTTAGTCG AGGTTAAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT
   61 GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC AAGGGCGAGG
  121 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA
  181 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT
  241 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT
  301 ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT
  361 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT
  421 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
  481 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA
  541 ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA
  601 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
  661 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG
  721 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
  781 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AAGCGGCCGG CCGCCAGCAC
  841 AGTGGTCGAC CGGGCCGCCA GCACAGTGGT CGATCGAGGG GGGGCCCGCG ATTAGTCCAA
  901 TTTGTTAAAG ACAGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG
  961 CTGAAGCCTA TAGAGTACGA GCCATAGATA GAATAAAAGA TTTTATTTAG TCTCCAGAAA
 1021 AATGGGGGGA ATGAAAGACC CCATCTTGTT AGTTTGGCAG CTAGCTTAGT TACGGCAATT
 1081 TTGGCAGCAT GCAAAATACA TACTGAGATA GAGCAAGTTC AGATCCAATG CTAAGTAACA
1141 GAGAAGACAG CAGGAT
//
Primer: pMFG-1 ()
Sequence file: RDB12172_A5Esc.seq check
>12172_12172_A5Es_pMFG-1_E07_13.ab1
    1 GAATCCGAGA GGGCTGTTAG TACTCTTGTC ATGTCAGGGT CAGGGAAGTT TACAAGGGGC
   61 GGGGGTGCCC CATATAAGAT CTCATATGGC CATATGGGGG CGCCTAGAGA AGGAGTGAGG
  121 GCTGGATAAA GGGAGGATCG AGGCGGGGTC GAACGAGGAG GTTCAAGGGG GAGAGACGGG
  181 GCGGATGGAG GAAGAGGAGG CGGAGGCTTA GGGTGTACAA AGGGCTTGAC CCAGGGAGGG
  241 GGGTCAAAAG CCAAGGCTTC CCAGGTCACG ATGTAGGGGA CCTGGTCTGG GTGTCCATGC
  301 GGGCCAGGTG AAAAGACCTT GATCTTAACC TGGGTGATGA GGTCTCGGTT AAAGGTGCCG
  361 TCTCGCGGCC ATCCGACGTT AAAGGTTGGC CATTCTGCAG AGCAGAAGGT AACCCAACGT
  421 CTCTTCTTGA CATCTACCGA CTGGTTGTGA GCGATCCGCT CGACATCTTT CCAGTGACCT
  481 AAGGTCAAAC TTAAGGGAGT GGTAACAGTC TGGCCCTAAT TTTCAGACAA ATACAGAAAC
  541 ACAGTCAGAC AGAGACAACA CAGAACGATG CTGCAGCAGA CAAGACGCGC GGCGCGGCTT
  601 CGGTCCCAAA CCGAAAGCAA AAATTCAGAC GGAGGCGGGA ACTGTTTTAG GTTCTCGTCT
  661 CCTACCAGAA CCACATATCC CTCCTATTAG GGGGGTGCAC CAAAGAGTCC AAAACGATCG
  721 GGATTTTTGG ACTCAGGTCG GGCCACAAAA ACGGCCCCCG AAGTCCCTGG GACGTCTCCC
  781 AGGGTTGCGG CCGGGTGTTC CGAACTCGTC AGTTCCACCA CGGGTCCGCC AGATACAGAG
  841 CTAGTTAGCT AACTAGTACA GACGCAGGCG CAAACATTAG ATGCCGGCAC AAACACGCAC
  901 AAAGACAGAG ACAGACACGA AACGACCGCT GGCCAGCTTA CCTCCCGGCG GGGGGGGTCG
  961 GTGGTCCCTG GGGCAGGGGT CTCCAAATCT CGGTGGAACC TCCCAAATGA AAGACCCCCG
 1021 AGGTGGGCAG TCAATCAATC TGAGGAGACC CTCCCAAGGA TCAGCGAGTC CACGATTCGG
 1081 GATGCAAACA GCAGAGGCTT TATTGGGGAA TACGGTACCT GGGCGAACTC AGTCCATCCG
1141 TAGACTTGAC GCCGCCGGAG CTGGAGGGGG TGTGTGGGGC CTCCAT
//
Primer: Amp_R ()
Sequence file: RDB12172_A5Esd.seq check
>12172_12172_A5Es_Amp_R_F07_16.ab1
    1 TTCGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGCATAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG
  181 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAAAAGTTC AGATCAAGGT CAGGAACAGA
  241 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
  301 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
  361 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA
  421 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
  481 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
  541 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGACTGAGT
  601 CGCCCGGGTA CCCGTATTCC CAATAAAGCC TCTTGCTGTT TGCATCCGAA TCGTGGACTC
  661 GCTGATCCTT GGGAGGGTCT CCTCAGATTG ATTGACTGCC CACCTCGGGG GTCTTTCATT
  721 TGGAGGTTCC ACCGAGATTT GGAGACCCCT GCCCAGGGAC CACCGACCCC CCCGCCGGGA
  781 GGTAAGCTGG CCAGCGGTCG TTTCGTGTCT GTCTCTGTCT TTGTGCGTGT TTGTGCCGGC
  841 ATCTAATGTT TGCGCCTGCG TCTGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC
  901 CGTGGTGGAA CTGACGAGTT CGGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC
  961 TTCGGGGGGC CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT
 1021 TTGGGTGCAC CCCCCTAATA GGAGGGATAT GTGGTTCTGG GTAGGAGACG AGAACCCTAA
 1081 AACAGTTCCG CTCGTCTGAT TTTTGCCTTT CGCTTGGAAC GAAGCCGCGA CGCGCGTCAT
1141 GGTCTGCCTT GCAGCATCGA TCTGTGATTG ATCTTCGTGA TCCTTGGA
//
Primer: EGFP-C ()
Sequence file: RDB12172_A5Ese.seq check
>12172_12172_A5Es_EGFP-C_G07_19.ab1
    1 CAGGTACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT
   61 CGACCGGGCC GCCAGCACAG TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT
  121 AAAGACAGGA TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG
  181 CCTATAGAGT ACGAGCCATA GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG
  241 GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG
  301 GCATGGAAAA TACATAACTG AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC
  361 AGCAGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC
  421 AAGAACAGTT GGAACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG
  481 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT
  541 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT
  601 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA
  661 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG
  721 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC
  781 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACATGCAGC
  841 ATGTATCAAA ATTAATTTGG TTTTTTTTTC TTAAGTATTT ACATTAAATG GCCATAGTTG
  901 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT
  961 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
 1021 TCAAAGGCGG TAATACGGTT ATCCACAGAA TTCATGGATA ACGCAGGAAA GGACATGTGA
 1081 GCAAACGCAG CCAACGTAGG AACGTTAAGG GCGCGATTGC TGGGCGTTTT TTTCCAATAA
1141 GG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]