Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMYs-Tead1-VP16-IRES-EGFP

Retroviral vector of mouse Tead1, constitutive active

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12172_A5Esp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro(PFB-5') () Sequence file: RDB12172_A5Esa.seq
>12172_12172_A5Es_5'Retro(PFB-5')_C07_07.ab1     1 CGGGTCGTTC GAATATTGCG CGGACTGCTA GTTATTAAGG ATCCCAGTGT GGTGGTACGG    61 GAATTCCTGC AGGCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTAA ATTCCACCAT   121 GGACTACAAG GACGACGATG ACAAGGGATC CGAATTCGAG CTCCGTCGAA TGGAACAGAA   181 ACTGATTAGC GAAGAAGATC TGAACGAAGG CCGCATGGAG CCCAGCAGCT GGAGCGGCAG   241 CGAGAGCCCT GCCGAAAACA TGGAAAGGAT GAGCGACTCG GCAGATAAGC CGATTGACAA   301 CGACGCGGAG GGCGTCTGGA GTCCTGATAT TGAGCAGAGT TTCCAGGAGG CCCTGGCTAT   361 CTATCCGCCG TGTGGGAGGA GAAAAATCAT CTTATCAGAC GAAGGCAAAA TGTATGGTAG   421 AAATGAATTG ATAGCCAGAT ACATCAAACT CAGGACGGGA AAGACAAGGA CCAGGAAGCA   481 GGTGTCTAGT CACATTCAGG TTCTTGCCAG AAGGAAATCT CGTGATTTTC ATTCCAAGCT   541 GAAGGATCAG ACTGCCAAGG ACAAGGCCCT GCAGCACATG GCTGCCATGT CATCAGCCCA   601 GATCGTCTCG GCTACTGCCA TCCACAACAA GCTGGGGCTG CCTGGGATTC CACGCCCCAC   661 CTTCCCGGCC CCCCCGACCG ATGTCAGCCT GGGGGACGAG CTCCACTTAG ACGGCGAGGA   721 CGTGGCGATG GCGCATGCCG ACGCGCTAGA CGATTTCGAT CTGGACATGT TGGGGGACGG   781 GGATTCCCCG GGTCCGGGAT TTACCCCCCA CGACTCCGCC CCCTACGGCG CTCTGGATAT   841 GGCCGACTTC GAGTTTGAGC AGATGTTTAC CGATGCCCTT GGAATTGACG AGTACGGTGG   901 GTAGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG ACTATGGGCA CCAAAGAACC   961 CTGTAAACGT TATCTTTTTA AATTGATGTG CACAAATAAA GTTGGAAGAA AATCACATCA  1021 AATATACAAA ACATATAGAC ATAAAGACAT AAAATAAAAT TCCGCCACTT CATTACGCGC  1081 TGTGTGGGCG TATCCTTCGA GTCTTAGAGA GTCTCGAGGC TGGCGCGGCC CGGACGTACG 1141 TAGCTCGTCC TCTTATCCAT CT //
Primer: IRES-F () Sequence file: RDB12172_A5Esb.seq
>12172_12172_A5Es_IRES-F_D07_10.ab1     1 GGATCTTATG TGTTTAGTCG AGGTTAAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT    61 GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC AAGGGCGAGG   121 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA   181 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT   241 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT   301 ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT   361 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT   421 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA   481 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA   541 ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA   601 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA   661 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG   721 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG   781 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AAGCGGCCGG CCGCCAGCAC   841 AGTGGTCGAC CGGGCCGCCA GCACAGTGGT CGATCGAGGG GGGGCCCGCG ATTAGTCCAA   901 TTTGTTAAAG ACAGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG   961 CTGAAGCCTA TAGAGTACGA GCCATAGATA GAATAAAAGA TTTTATTTAG TCTCCAGAAA  1021 AATGGGGGGA ATGAAAGACC CCATCTTGTT AGTTTGGCAG CTAGCTTAGT TACGGCAATT  1081 TTGGCAGCAT GCAAAATACA TACTGAGATA GAGCAAGTTC AGATCCAATG CTAAGTAACA 1141 GAGAAGACAG CAGGAT //
Primer: pMFG-1 () Sequence file: RDB12172_A5Esc.seq
>12172_12172_A5Es_pMFG-1_E07_13.ab1     1 GAATCCGAGA GGGCTGTTAG TACTCTTGTC ATGTCAGGGT CAGGGAAGTT TACAAGGGGC    61 GGGGGTGCCC CATATAAGAT CTCATATGGC CATATGGGGG CGCCTAGAGA AGGAGTGAGG   121 GCTGGATAAA GGGAGGATCG AGGCGGGGTC GAACGAGGAG GTTCAAGGGG GAGAGACGGG   181 GCGGATGGAG GAAGAGGAGG CGGAGGCTTA GGGTGTACAA AGGGCTTGAC CCAGGGAGGG   241 GGGTCAAAAG CCAAGGCTTC CCAGGTCACG ATGTAGGGGA CCTGGTCTGG GTGTCCATGC   301 GGGCCAGGTG AAAAGACCTT GATCTTAACC TGGGTGATGA GGTCTCGGTT AAAGGTGCCG   361 TCTCGCGGCC ATCCGACGTT AAAGGTTGGC CATTCTGCAG AGCAGAAGGT AACCCAACGT   421 CTCTTCTTGA CATCTACCGA CTGGTTGTGA GCGATCCGCT CGACATCTTT CCAGTGACCT   481 AAGGTCAAAC TTAAGGGAGT GGTAACAGTC TGGCCCTAAT TTTCAGACAA ATACAGAAAC   541 ACAGTCAGAC AGAGACAACA CAGAACGATG CTGCAGCAGA CAAGACGCGC GGCGCGGCTT   601 CGGTCCCAAA CCGAAAGCAA AAATTCAGAC GGAGGCGGGA ACTGTTTTAG GTTCTCGTCT   661 CCTACCAGAA CCACATATCC CTCCTATTAG GGGGGTGCAC CAAAGAGTCC AAAACGATCG   721 GGATTTTTGG ACTCAGGTCG GGCCACAAAA ACGGCCCCCG AAGTCCCTGG GACGTCTCCC   781 AGGGTTGCGG CCGGGTGTTC CGAACTCGTC AGTTCCACCA CGGGTCCGCC AGATACAGAG   841 CTAGTTAGCT AACTAGTACA GACGCAGGCG CAAACATTAG ATGCCGGCAC AAACACGCAC   901 AAAGACAGAG ACAGACACGA AACGACCGCT GGCCAGCTTA CCTCCCGGCG GGGGGGGTCG   961 GTGGTCCCTG GGGCAGGGGT CTCCAAATCT CGGTGGAACC TCCCAAATGA AAGACCCCCG  1021 AGGTGGGCAG TCAATCAATC TGAGGAGACC CTCCCAAGGA TCAGCGAGTC CACGATTCGG  1081 GATGCAAACA GCAGAGGCTT TATTGGGGAA TACGGTACCT GGGCGAACTC AGTCCATCCG 1141 TAGACTTGAC GCCGCCGGAG CTGGAGGGGG TGTGTGGGGC CTCCAT //
Primer: Amp_R () Sequence file: RDB12172_A5Esd.seq
>12172_12172_A5Es_Amp_R_F07_16.ab1     1 TTCGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG    61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC   121 CTGCATAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG   181 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAAAAGTTC AGATCAAGGT CAGGAACAGA   241 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC   301 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG   361 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA   421 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC   481 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG   541 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGACTGAGT   601 CGCCCGGGTA CCCGTATTCC CAATAAAGCC TCTTGCTGTT TGCATCCGAA TCGTGGACTC   661 GCTGATCCTT GGGAGGGTCT CCTCAGATTG ATTGACTGCC CACCTCGGGG GTCTTTCATT   721 TGGAGGTTCC ACCGAGATTT GGAGACCCCT GCCCAGGGAC CACCGACCCC CCCGCCGGGA   781 GGTAAGCTGG CCAGCGGTCG TTTCGTGTCT GTCTCTGTCT TTGTGCGTGT TTGTGCCGGC   841 ATCTAATGTT TGCGCCTGCG TCTGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC   901 CGTGGTGGAA CTGACGAGTT CGGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC   961 TTCGGGGGGC CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT  1021 TTGGGTGCAC CCCCCTAATA GGAGGGATAT GTGGTTCTGG GTAGGAGACG AGAACCCTAA  1081 AACAGTTCCG CTCGTCTGAT TTTTGCCTTT CGCTTGGAAC GAAGCCGCGA CGCGCGTCAT 1141 GGTCTGCCTT GCAGCATCGA TCTGTGATTG ATCTTCGTGA TCCTTGGA //
Primer: EGFP-C () Sequence file: RDB12172_A5Ese.seq
>12172_12172_A5Es_EGFP-C_G07_19.ab1     1 CAGGTACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT    61 CGACCGGGCC GCCAGCACAG TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT   121 AAAGACAGGA TATCAGTGGT CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG   181 CCTATAGAGT ACGAGCCATA GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG   241 GGAATGAAAG ACCCCACCTG TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG   301 GCATGGAAAA TACATAACTG AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC   361 AGCAGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC   421 AAGAACAGTT GGAACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG   481 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT   541 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT   601 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA   661 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG   721 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC   781 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CACATGCAGC   841 ATGTATCAAA ATTAATTTGG TTTTTTTTTC TTAAGTATTT ACATTAAATG GCCATAGTTG   901 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT   961 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC  1021 TCAAAGGCGG TAATACGGTT ATCCACAGAA TTCATGGATA ACGCAGGAAA GGACATGTGA  1081 GCAAACGCAG CCAACGTAGG AACGTTAAGG GCGCGATTGC TGGGCGTTTT TTTCCAATAA 1141 GG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl