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Ecad-Flag/pCAG

Expression clone of mouse E-cadherin

Catalog number RDB12215
Resource name Ecad-Flag/pCAG
Clone info. Expression clone of mouse E-cadherin, FLAG-tagged.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 6.1 kb
Gene/insert name Mouse E-Cadherin cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Mouse E-Cadherin

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12215 Ecad-Flag/pCAG DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Ecad-Flag/pCAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12215).

          Reference section:

          Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12215_A3Iip1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1 (Pr0164)
          Region: insert 5'(Ecad 5')
          Sequence file: RDB12215_A3Iia.seq check
          >12215_1_pAxCAF1_B05_05.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNTTCTT CTTTTTCCNA CNNCTCCTGG
             61 GCAACGTGCT GGTTATTGNN NNNNNNNNNN NNTTTGGCAA AGAATTGGTA CCGATGATCT
            121 GCCTTACAAT TAATACATAA CCTTTTGTAT CATACACATA CGATTTAGGT GACACTATAG
            181 AATTCGGGCC GGCCTAACCC GGCCCTGCCC GACCGCACCC GAGCTCAGTG TTTGCTCGGC
            241 GTCTGCCGGG TCCGCCATGG GAGCCCGGTG CCGCAGCTTT TCCGCGCTCC TGCTCCTGCT
            301 GCAGGTCTCC TCATGGCTTT GCCAGGAGCT GGAGCCTGAG TCCTGCAGTC CCGGCTTCAG
            361 TTCCGAGGTC TACACCTTCC CGGTGCCGGA GAGGCACCTG GAGAGAGGCC ATGTCCTGGG
            421 CAGAGTGAGA TTTGAAGGAT GCACCGGCCG GCCAAGGACA GCCTTCTTTT CGGAAGACTC
            481 CCGATTCAAA GTGGCGACAG ACGGCACCAT CACAGTGAAG CGGCATCTAA AGCTCCACAA
            541 GCTGGAGACC AGTTTCCTCG TCCGCGCCCG GGACTCCAGT CATAGGGAGC TGTCTACCAA
            601 AGTGACGCTG AAGTCCATGG GGCACCNNNN NCACCGGCAC CACCACCGCG ACCCTGCCTC
            661 TGAATCCAAC CCAGAGCTGC TCATGTTTCC CAGCGTGTAC CCAGGTCTCN NAAGACAGAA
            721 ACGAGACTGG GTCATCCCTC CCATCAGCTG CCCCGAAAAT GAAAAGGGTG AATTCCCAAA
            781 GAACCTGNTT CANATCNAAT CCAACAGGGA CAAAGAAACN AAGGNTTTCT ACNGCATCAC
            841 CGGCNNANNG AGCTGACAAN NCCCCCGTTG GCGTTTNCAT CNTTGANAGG GAGACAGGNN
            901 TGGCTGAAAA GTGACANNNN NNTCNGGNAT AGAGAANNCN NTTGNCNAAG TACATCCTCN
            961 ANTNCTCATG NCNNNGTCAT CAAANGGGGG AANCNGNNGG NANNNNNCCC NNGGNNNANN
          1021 NNNATNNNNN NNNNCANNNC NNANNGNNNA NNNNNNNNNN
          //
          Primer: pCA-bgter (Pr0066)
          Region: insert 3'(FLAG,Ecad 3')
          Sequence file: RDB12215_A3Iib.seq check
          >12215_12215_A3Ii_1_pCA-bgter_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN TTTTGGNNNA NGNNNNNGNN CTNNGTGGTA TTTGTGAGCC
             61 AGGGCATTGG CCACACCAGC CACCACCTTC TGATAGGCAG CCTGCACCTG AGGAGTGAAT
            121 TGTCGACGAT ATCTTATTTG TCATCGTCGT CCTTGTAGTC TCTAGAGTCG TCCTCACCAC
            181 CGCCGTACAT GTCCGCCAGC TTCTTGAATC GGTTGCCCCA CTCGTTCAGA TAATCGTAGT
            241 CCTGGTCCTG ATCCGACTCA GAGGAGTTCA GTGAGCTCAG GCTAGCGGCT TCAGAACCAC
            301 TGCCCTCGTA ATCGAACACC AACAGAGAGT CGTAAGGGGG TGCCGTGGGG TCGCTGTCGG
            361 CTGCCTTCAG GTTTTCATCG ATGAAGTTTC CAATTTCATC AGGATTGGCA GGACGGGGAC
            421 GATACTGGGG CACGCTCATG AGGGTGGGAG CCACATCATT TCGAGTCACT TCCGGTCGGG
            481 CATCCAGGCC CCTGTGCAGC TGGCTCAAAT CAAAGTCCTG GTCTTCTTCT CCACCTCCTT
            541 CTTCATCATA GTAATACACA TTGTCCCGGG TATCATCATC TGGTGGCAGC AGGGGCTCTT
            601 TGACCACCGT TCTCCTCCGT AGAAACAGTA GGAGCAGCAG GATCAGAATC AGCAGGGCGA
            661 GGATCCCTCC AAGGANTCCG AGGATGGCNA NGAACTTGCA ATCCTGCTGN NNNNATTCCC
            721 GCCTTCATGC AGTTGNTGAN CNGTCCCNTN NNNGTCACAC ACATNGGACG TCCNAANNTG
            781 GNCNCCTGGG TCTTTGTTCT GGTTATCNNC GAGNTTNNAN ANNGNNNTTT TGNANTCGCC
          841 NNNNNNNNNN
          //
          Primer: phRLR1 (Pr0219)
          Region: NeoR
          Sequence file: RDB12215_A3Iic.seq check
          >12215_12215_A3Ii_1_phRLR1_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTTTGATGNN TTTAGAAAAA TAAACAAATA GGGGTTCCGC
             61 GCACATTTCC CCGAAAAGTG CCACCTGGGT CGAGCAGTGT GGTTTTCAAG AGGAAGCAAA
            121 AAGCCTCTCC ACCCAGGCCT GGAATGTTTC CACCCAATGT CGAGCAGTGT GGTTTTGCAA
            181 GAGGAAGCAA AAAGCCTCTC CACCCAGGCC TGGAATGTTT CCACCCAATG TCGAGCAAAC
            241 CCCGCCCAGC GTCTTGTCAT TGGCGAATTA ATTCGAACAC GCANATGCAG TCGGGGCGGC
            301 GCGGTCCCAG GTCCACTTCG CATATTAAGG TGACGCGTGT GGCCTCGAAC ACCGAGCGAC
            361 CCTGCAGCCA ATATGGGATC GGCCATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC
            421 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT
            481 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG
            541 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG
            601 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA
            661 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA
            721 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC
            781 GACNNCCAAG CGAAACANNN NNTCNAGCGN NNANGNACTC GGATGGAAGC CGGTCTTGNC
            841 GATCNNGANG ATCTGGANNA ANANCATCNN GGGGCTCGCG CCNGCCNAAC TNTTCNCCAG
            901 GNNNNNNGGC NCNNATGCCC NANGGNNNNG ATNTCNNCNN GACCCNNGGN NNATGNNNNG
          961 CTTGNCNAAT ANCNNNNNNN AAANNNNNNN CNTTNTNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

          2024.03.26

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