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pcDNA3.1-His-Gli2

Expression vector of mouse Gli2 cDNA

Catalog number RDB08065
Resource name pcDNA3.1-His-Gli2
Clone info. Expression vector of mouse Gli2 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Gli2 cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Mouse Gli2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08065 pcDNA3.1-His-Gli2 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17729_A9Kfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, 6xHis, T7 tag, Xpress tag, insert 5'
          Sequence file: RDB17729_A9Kfa.seq check
          >08065_17729_A9Kf_3_CMV_Forward_D02_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGGCG GTAGGCGNGN NNNGTGGGAG
             61 GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA
            121 ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTACCA
            181 TGGGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA
            241 TGGGTCGGGA TCTGTACGAC GATGACGATA AGGTACCTAG GATCCAGTGT GGTGGAATTC
            301 TGCAGATCTC TGAGATGGAG ACTTCTGCCC CAGCCCCTGC ACTGGAGAAG AAAGAAGCCA
            361 AGAGTGGTCT CTTGGAGGAC AGCAGCTTCC CCGACCCAGG GAAAAAGGCC TGTCCTCTGG
            421 CGGTGGCCGC AGCTGTAGCC GCCCACGGAG TTCCTCAGCA GCTCCTGCCG GCTTTCCACG
            481 CGCCTTTGCC GATTGACATG AGACACCAGG AGGGAAGGTA CCATTATGAC CCTCACTCTG
            541 TCCACAGTGT ACACGGGCCT CCCACCCTAA GTGGCAGCCC TGTCATCTCA GATATCTCCT
            601 TGATACGACT TTCTCCACAC CCTGCTGGCC CTGGAGAGTC ACCCTTCAGC GCCCACCACC
            661 CCTACGTGAA CCCCCATATG GAGCACTACC TCCGGTCTGT GCACAGCAGC CCCACACTCT
            721 CAATGATCTC TGCCGCCAGG GGCCTCAGCC CTGCTGATGT GGCCCACGAA CATCTGAAAG
            781 ANAGGGGACT CTTTAGCCTC GCAGCCCCAG GCACCAACCC TTCANANTAT TACCACCAGA
            841 TGANCCTCAT GGCAAGCCAC CCCACCCCTT ATGGGGNCCT TCTAATGCNN NNCGGGGGNG
            901 CTGCTANCGC ANCCCATCTC CATGACTACN TCAACCCTGN GGATGCNTCA CGATTCTCTA
            961 NTCCACGNGT GANCCCNCGN NTGANCCNCA NGCGGGCTCT NNNNNTCNNC CCGCNNNCNN
          1021 ANGNCANCNN CNANNNNNNA NCNNNNNNNN NN
          //
          Primer: bGH-M_R
          Region: BGH p(A), insert 3'
          Sequence file: RDB17729_A9Kfb.seq check
          >08065_17729_A9Kf_3_bGH-M_R_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGGNNNNN NNNNNACAGN NGGCTGGCAA CTAGAAGGCA
             61 CANGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCTTAGGT
            121 CATCATGTTT AAAAACTTGC TCTCTTCAGC CAGGCTGGTG AGCATGGAAC TCATGTCGCC
            181 CACGGCCATG TTGCTGATGC CCGCAGGGAT GGAGGGCAGT GTCAAGGAAT TCCGGGGTGT
            241 GGTGAGGCGT GAGGAATTCT GGGAGAGATT GTGGAGAAGG GTTGGGCTCA GTGCACCAGA
            301 AAACAAACTC GAGTGATCAC CATCATCCAT GATGGCATCA AAGTCAATCT GGGGGGGCTC
            361 CAGGAGCTGG GAGTCCACGG TGCTAGACAC CTGGTTGACT CCCGGTGAAG GCAAAGGCTC
            421 AGGCTGGATA CTGTCTGAGC AAGCTTGTGG TTGTGGCGGC TGGGGCTGCA CGGCTGGGCA
            481 GCCTCCATTC TGTTCATACA TGTGGATCTG GCCATAGTAG TATAGCGTGT TCTGATCTTG
            541 TGCCCGCCCT GTGTCCTGAG ATGGCTGAGG AGCTAGGGAT ACCATGGTCC CTGCTGGTCC
            601 CTTGGGCATA GCTTCTGCTG TCTCTTGGTT GGCTGATGTG ACCATGGCTT GGCCAGTGGT
            661 CCTGGCATAC AACTGCTGTT GCCCAGAACG TACTCCACGG TGACGGCTGG CCGTGTTGGG
            721 TTCCAGGGGT GGCCGANGCT GCATGAGACC AAAGCCCGCG TTCTGCTGGG ACTCCATGAA
            781 GCCAGGCTGA TGGGATGACA GANNGTTGGA NCCCTGNTGG TANCTGTCCT GCTGGGGATA
            841 GCTCNNGANT GGGTGANGTT GTGANNCCGC ANCCNNGGAN GAANTGGGGN NCCCNNGCTC
            901 CNGTTGATGN NNNNNCATGA TGGTGGNANT GGGGNNAACT TNNCNTTTGC TGANGGANNC
            961 CCNNGNNGNN NGNNTNNNNT GNNNTATTNN TGNNNNNNNN NAAANNNGNN NCNNNNANNC
          1021 NCNNNNNNTG NTNNGNNNNN NNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori, NeoR
          Sequence file: RDB17729_A9Kfc.seq check
          >08065_17729_A9Kf_3_SV40pro_ori_F_F02_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNGNNN CCTCTGNCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT
             61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
            121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
            181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
            241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
            301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
            361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
            421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
            481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
            541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
            601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
            661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
            721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
            781 NCGGCTGGGT GTGGCGGANC GCTATCANGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
            841 ANAGCTTGNC GGCGAATGGG CTGANCGCTT CCTCGTGCTT TACNGNATCG CCGCTCCCGA
            901 TTCGCANCGC ATCNCCTTCT ATCGCCTTCT TGANNANTTC TTCTGANCGG GNNTCTGGGG
            961 TNCNAAATGA CCNANCNAGC NACNCCNNNN NTGNCNTCNC GANNTTTNNA NTNNNNNNNN
          1021 NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
          user report Mikami, Y., GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas. J. Cancer Res. Clin. Oncol. 143 (8): 1381-1393 (2017). PMID 28342001.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl