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pcDNA3.1-His-Gli2

Expression vector of mouse Gli2 cDNA

Catalog number RDB08065
Resource name pcDNA3.1-His-Gli2
Clone info. Expression vector of mouse Gli2 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Gli2 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Gli2 (NCBI Gene 14633) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08065 pcDNA3.1-His-Gli2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08065_A3Fo.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08065_A3Foa.seq
>08065_08065_A3Fo_T7_20bp_A05_02.ab1
    1 CAGAGGCTGG CTAGCGTTTA ACTTAAGCTT ACCATGGGGG GTTCTCATCA TCATCATCAT
   61 CATGGTATGG CTAGCATGAC TGGTGGACAG CAAATGGGTC GGGATCTGTA CGACGATGAC
  121 GATAAGGTAC CTAGGATCCA GTGTGGTGGA ATTCTGCAGA TCTCTGAGAT GGAGACTTCT
  181 GCCCCAGCCC CTGCACTGGA GAAGAAAGAA GCCAAGAGTG GTCTCTTGGA GGACAGCAGC
  241 TTCCCCGACC CAGGGAAAAA GGCCTGTCCT CTGGCGGTGG CCGCAGCTGT AGCCGCCCAC
  301 GGAGTTCCTC AGCAGCTCCT GCCGGCTTTC CACGCGCCTT TGCCGATTGA CATGAGACAC
  361 CAGGAGGGAA GGTACCATTA TGACCCTCAC TCTGTCCACA GTGTACACGG GCCTCCCACC
  421 CTAAGTGGCA GCCCTGTCAT CTCAGATATC TCCTTGATAC GACTTTCTCC ACACCCTGCT
  481 GGCCCTGGAG AGTCACCCTT CAGCGCCCAC CACCCCTACG TGAACCCCCA TATGGAGCAC
  541 TACCTCCGGT CTGTGCACAG CAGCCCCACA CTCTCAATGA TCTCTGCCGC CAGGGGCCTC
  601 AGCCCTGCTG ATGTGGCCCA CGAACATCTG AAAGAGAGGG GACTCTTTAG CCTCGCAGCC
  661 CCAGGCACCA ACCCTTCAGA CTATTACCAC CAGATGACCC TCATGGCAAG CCACCCCACC
  721 CCTTATGGGG ACCTTCTAAT GCAGAGCGGG GGTGCTGCTA GCGCACCCCA TCTCCATGAC
  781 TACCTCAACC CTGTGGATGC ATCACGATTC TCTAGTCCAC GTGTGACCCC ACGACTGAGC
  841 CGCAAGCGGG CTCTGTCCAT CTCCCCGCTC TCAGATGCCA GCCTCGACCT ACAACGCATG
  901 ATTCGGACCT CTCCCAACTC GCTGGTAGCT TACATCAACA ACTCCAGGAG CAGCTCAGCA
  961 GCCAGTGGCT CTTATGGACA TCTGTCTGCT GGTGCCCTCA GCCCAGCCCT TCACTTTTTC
 1021 CCCACCCCAT CAAATCCCTG TGGTCCTACC CAAGCAGATC CCTGAGGCCA GCAGCGGGGT
1081 CTGGAGCTCA GCATTTGGAC ACACACCACC ACCCCCCTGT GAACCCCAAG C
//
Primer: BGH_rev RDB08065_A3Fob.seq
>08065_08065_A3Fo_BGHrev_B05_05.ab1
    1 GGTTCGGGGA CTCTAGACTC GAGCGGCCGC TTAGGTCATC ATGTTTAAAA ACTTGCTCTC
   61 TTCAGCCAGG CTGGTGAGCA TGGAACTCAT GTCGCCCACG GCCATGTTGC TGATGCCCGC
  121 AGGGATGGAG GGCAGTGTCA AGGAATTCCG GGGTGTGGTG AGGCGTGAGG AATTCTGGGA
  181 GAGATTGTGG AGAAAGGTTG GGCTCAGTGC ACCAGAAAAC AAACTCGAGT GATCACCATC
  241 ATCCATGATG GCATCAAAGT CAATCTGGGG GGGCTCCAGG AGCTGGGAGT CCACGGTGCT
  301 AGACACCTGG TTGACTCCCG GTGAAGGCAA AGGCTCAGGC TGGATACTGT CTGAGCAAGC
  361 TTGTGGTTGT GGCGGCTGGG GCTGCACGGC TGGGCAGCCT CCATTCTGTT CATACATGTG
  421 GATCTGGCCA TAGTAGTATA GCGTGTTCTG ATCTTGTGCC CGCCCTGTGT CCTGAGATGG
  481 CTGAGGAGCT AGGGATACCA TGGTCCCTGC TGGTCCCTTG GGCATAGCTT CTGCTGTCTC
  541 TTGGTTGGCT GATGTGACCA TGGCTTGGCC AGTGGTCCTG GCATACAACT GCTGTTGCCC
  601 AGAACGTACT CCACGGTGAC GGCTGGCCGT GTTGGGTTCC AGGGGTGGCC GAGGCTGCAT
  661 GAGACCAAAG CCCGCGTTCT GCTGGGACTC CATGAAGCCA GGCTGATGGG ATGACAGAAG
  721 GTTGGAGCCC TGTTGGTAGC TGTCCTGCTG GGGATAGCTC AGGACTGGGT GAGGTTGTGA
  781 CACCGCAGCC AGGGATGAAT TGGGGACCCC GTGCTCCCGT TGATGGCCAT TCATGATGGT
  841 GGCATTGGGG ACAACTTCCA TTTGCTGAGG GAGTCCCAGG TGGCTGGGCT TGGCTGCCAT
  901 ATTGCTGCAG GAGGGAGAAA ACTGGTTCAG AGATCCGCTG ACAATGTTTG GGCTCAGCTG
  961 GGGGGTGGGC TTGGCTGTAG CTGGCCCTGC GGGCTCATGG GCCGCACTCA TACCCAGGCA
 1021 CTGACTGCCC TGCAGCTGGG CTGTCGAGGG CTGTTTGCGT TGTTAGCTCC TCTTGATGCA
 1081 GAGGGGCTTC TTCGAGCCTG GTAGTTGCCG CCTGGAATGC TTGATCTTAG AACCCTTCCC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
user report Mikami, Y., GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas. J. Cancer Res. Clin. Oncol. 143 (8): 1381-1393 (2017). PMID 28342001.

2018.10.22

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