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pcDNA3.1-His-Gli2 (#RDB08065)

Expression vector of mouse Gli2 cDNA

Clone info. Expression vector of mouse Gli2 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Gli2 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08065 pcDNA3.1-His-Gli2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-His-Gli2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08065).

Reference section:

Sasaki, H., Nishizaki, Y., Hui, C., Nakafuku, M., Kondoh, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17729_A9Kfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB17729_A9Kfa.seq check
>08065_17729_A9Kf_3_CMV_Forward_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGGCG GTAGGCGNGN NNNGTGGGAG
   61 GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA
  121 ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTACCA
  181 TGGGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA
  241 TGGGTCGGGA TCTGTACGAC GATGACGATA AGGTACCTAG GATCCAGTGT GGTGGAATTC
  301 TGCAGATCTC TGAGATGGAG ACTTCTGCCC CAGCCCCTGC ACTGGAGAAG AAAGAAGCCA
  361 AGAGTGGTCT CTTGGAGGAC AGCAGCTTCC CCGACCCAGG GAAAAAGGCC TGTCCTCTGG
  421 CGGTGGCCGC AGCTGTAGCC GCCCACGGAG TTCCTCAGCA GCTCCTGCCG GCTTTCCACG
  481 CGCCTTTGCC GATTGACATG AGACACCAGG AGGGAAGGTA CCATTATGAC CCTCACTCTG
  541 TCCACAGTGT ACACGGGCCT CCCACCCTAA GTGGCAGCCC TGTCATCTCA GATATCTCCT
  601 TGATACGACT TTCTCCACAC CCTGCTGGCC CTGGAGAGTC ACCCTTCAGC GCCCACCACC
  661 CCTACGTGAA CCCCCATATG GAGCACTACC TCCGGTCTGT GCACAGCAGC CCCACACTCT
  721 CAATGATCTC TGCCGCCAGG GGCCTCAGCC CTGCTGATGT GGCCCACGAA CATCTGAAAG
  781 ANAGGGGACT CTTTAGCCTC GCAGCCCCAG GCACCAACCC TTCANANTAT TACCACCAGA
  841 TGANCCTCAT GGCAAGCCAC CCCACCCCTT ATGGGGNCCT TCTAATGCNN NNCGGGGGNG
  901 CTGCTANCGC ANCCCATCTC CATGACTACN TCAACCCTGN GGATGCNTCA CGATTCTCTA
  961 NTCCACGNGT GANCCCNCGN NTGANCCNCA NGCGGGCTCT NNNNNTCNNC CCGCNNNCNN
1021 ANGNCANCNN CNANNNNNNA NCNNNNNNNN NN
//
Primer: bGH-M_R (Pr0747)
Region: BGH p(A), insert 3'
Sequence file: RDB17729_A9Kfb.seq check
>08065_17729_A9Kf_3_bGH-M_R_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGGNNNNN NNNNNACAGN NGGCTGGCAA CTAGAAGGCA
   61 CANGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCTTAGGT
  121 CATCATGTTT AAAAACTTGC TCTCTTCAGC CAGGCTGGTG AGCATGGAAC TCATGTCGCC
  181 CACGGCCATG TTGCTGATGC CCGCAGGGAT GGAGGGCAGT GTCAAGGAAT TCCGGGGTGT
  241 GGTGAGGCGT GAGGAATTCT GGGAGAGATT GTGGAGAAGG GTTGGGCTCA GTGCACCAGA
  301 AAACAAACTC GAGTGATCAC CATCATCCAT GATGGCATCA AAGTCAATCT GGGGGGGCTC
  361 CAGGAGCTGG GAGTCCACGG TGCTAGACAC CTGGTTGACT CCCGGTGAAG GCAAAGGCTC
  421 AGGCTGGATA CTGTCTGAGC AAGCTTGTGG TTGTGGCGGC TGGGGCTGCA CGGCTGGGCA
  481 GCCTCCATTC TGTTCATACA TGTGGATCTG GCCATAGTAG TATAGCGTGT TCTGATCTTG
  541 TGCCCGCCCT GTGTCCTGAG ATGGCTGAGG AGCTAGGGAT ACCATGGTCC CTGCTGGTCC
  601 CTTGGGCATA GCTTCTGCTG TCTCTTGGTT GGCTGATGTG ACCATGGCTT GGCCAGTGGT
  661 CCTGGCATAC AACTGCTGTT GCCCAGAACG TACTCCACGG TGACGGCTGG CCGTGTTGGG
  721 TTCCAGGGGT GGCCGANGCT GCATGAGACC AAAGCCCGCG TTCTGCTGGG ACTCCATGAA
  781 GCCAGGCTGA TGGGATGACA GANNGTTGGA NCCCTGNTGG TANCTGTCCT GCTGGGGATA
  841 GCTCNNGANT GGGTGANGTT GTGANNCCGC ANCCNNGGAN GAANTGGGGN NCCCNNGCTC
  901 CNGTTGATGN NNNNNCATGA TGGTGGNANT GGGGNNAACT TNNCNTTTGC TGANGGANNC
  961 CCNNGNNGNN NGNNTNNNNT GNNNTATTNN TGNNNNNNNN NAAANNNGNN NCNNNNANNC
1021 NCNNNNNNTG NTNNGNNNNN NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR
Sequence file: RDB17729_A9Kfc.seq check
>08065_17729_A9Kf_3_SV40pro_ori_F_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNGNNN CCTCTGNCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT
   61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC
  121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
  181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
  241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
  301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
  361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
  421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
  481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC
  541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
  601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT
  661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA
  721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG
  781 NCGGCTGGGT GTGGCGGANC GCTATCANGA CATAGCGTTG GCTACCCGTG ATATTGCTGA
  841 ANAGCTTGNC GGCGAATGGG CTGANCGCTT CCTCGTGCTT TACNGNATCG CCGCTCCCGA
  901 TTCGCANCGC ATCNCCTTCT ATCGCCTTCT TGANNANTTC TTCTGANCGG GNNTCTGGGG
  961 TNCNAAATGA CCNANCNAGC NACNCCNNNN NTGNCNTCNC GANNTTTNNA NTNNNNNNNN
1021 NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [link to RRC of NBRP]
user_report Mikami, Y., GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas. J. Cancer Res. Clin. Oncol. 143 (8): 1381-1393 (2017). PMID 28342001. [link to RRC of NBRP]