Resource data sheet
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pcDNA3.1-pA83-Lats2KD

Expression clone of mouse Lats2 cDNA, kinase dead variant

Catalog number RDB12201
Resource name pcDNA3.1-pA83-Lats2KD
Clone info. Expression clone of mouse Lats2 cDNA. Catalytically inactive (kinase dead) variant. N-terminal HA-tag.
D768A mutation in kinase subdomain VII, corresponding human D809A mutation. The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Lats2 cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Lats2 (NCBI Gene 50523) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12201 pcDNA3.1-pA83-Lats2KD DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12201_A4Fup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12201_A4Fua.seq
>12201_12201_A4Fu_CMV-Foward_A09_03.ab1
    1 AGAGATTTTC ACCTTCCGCC CCAATTGAAG GCAAAATGGG CGGTAGGCGT GTACGGTGGG
   61 AGGTCTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
  121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCCGGCTA GTTAAGCTTG GTACCGAGCT
  181 CGGATCCACT AGTCCAGTGT GGTGGAATTG ATCCCAGTGT GGTGGTACGG GAATTCCTGC
  241 AGGATGTACC CATACGACGT TCCAGATTAC GCTGGTACCA TGAGGCCAAA GACTTTTCCT
  301 GCCACAACTT ACTCTGGAAA TAGCCGGCAG CGATTGCAAG AGATTCGAGA GGGGCTGAAG
  361 CAGCCATCCA AGGCTTCCAC CCAGGGGCTG CTGGTGGGAC CAAACAGTGA CACTTCCCTG
  421 GATGCCAAAG TCCTGGGGAG CAAAGATGCC TCCAGGCAGC AGCAAATGAG AGCCACCCCG
  481 AAGTTTGGAC CTTATCAAAA AGCTCTCAGG GAAATCCGAT ATTCCCTCCT GCCTTTTGCC
  541 AACGAGTCAG GCACTTCGGC AGCTGCAGAG GTGAACCGGC AGATGCTTCA GGAGTTGGTG
  601 AATGCGGGAT GTGACCAGGA GATGGCTGGC AGAGCGCTCA AGCAGACGGG CAGTAGGAGT
  661 ATCGAAGCTG CCTTGGAGTA CATCAGTAAG ATGGGCTACC TGGACCCCAG GAATGAGCAG
  721 ATTGTGCGAG TCATCAAGCA GACCTCCCCA GGAAAAGGGC CTGGCGCCCA CCCCGGTGAC
  781 TCGGCGGCCC AGTTTCGAGG GCACAGGGGA AGCACTCCCA TCCTACCACC AGCTGGGTGG
  841 TGCAAACTAC GAGGGCCCCG CCGCACTGGA GGAGATGCCG CGGCAATATT TTAGACTTTC
  901 TCTTCCCTGG AGCCGGAGCC GGGCACCCAA CGGTGCCCAG GCTCACCAGC ATCCTCCCAA
  961 AAGGGTAACA GCACAGCAGT AGAGCCAAGT GCGCCACTTT CCCGGGGCAC CACACTTATG
 1021 GGTCGGTGGT CCATCCTACT AATCGGAGCC AGCCCTGGTT ATGGGGGGTT GCCAAGCGCC
 1081 AAGTTCTCGT TTCCAGGAAA CCAAGAACGG CACCAAGATG CCCTTATTCC AAGCAATGGC
1141 CAAGGCCCAA GGGGGTGGCC T
//
Primer: GNP_primer#10 RDB12201_A4Fub.seq
>12201_12201_A4Fu_GNP_primer#10_B09_06.ab1
    1 TTATTGTGCA ATTCATTTAA AAGATAACGT TTACAGGTTC TTTGGTGCCC ATAGTCGAGC
   61 GGCCGCCACT GTGCTGGATA TCTGCAGAAT TTCGAGGTTA CACGTACACC GGCTGGCAGC
  121 CCTCGGCCGC ACCTTCCAAG TCCGCATCCC CTGGGTCTGC ACTCTCTGCG GGCTCTGAGG
  181 GCTTCGGGCA CCGGAAGGGA TAGCCATTGT CATCGAAGAA CCTGCGGAAG GTGAACTCAT
  241 AGAAGGCGTG CTCTGGATGC TTGCTGCTGG GGGAGGCCAG CGTGTCCCAG GCCTTGGCGC
  301 TCTCTCCGCT GGCCTCGTGC CAGGGGCTTT CTTCATCCAC CGGGTCAAAA TTGGAGGTGT
  361 CCATGGGGTG GCTGATGGTG GGGACGTAGG GTGCAGGCTG CTTTCGGATG TCACGGGAAA
  421 AGTCGATGGT GTTGAAGAAC GGGTGTGCCT TGAGGTCATC TGCCCCATCC CTGCCCAGGC
  481 GGCAGTCAGC CGCGCAGCAC AGCTTCGTGA TGAGGTCTCG GGCCTCAGCG CTGAGCCTCA
  541 CCTGCGTAGG GATATGCAGC GTGCTCTCCC AGTTGATCAC CTTCAGCTGC GTCTCTGTGG
  601 GGGTGGGGGC CAAGAAAGGC GGCTGCCCAA CCAGCATCTC AAAGAGAATC ACACCGACGC
  661 TCCACCAGTC ACAGAGCTGC GTGTACCCTT TGCGGAGAAG CACCTCCGGA GCGATGTAAT
  721 TTGGTGTCCC GACAAGAGAA TGTGCCAGCA CCTCTGGTGC TGCTTCTGCG CCCTCTGCTC
  781 CAGGGTCTTT AACCTGTCTC CACAGCGACA GTTGGAAACA TCGTCCCAGA GTCACCGGGC
  841 TCATGCTGTC TGTCTCATGT GATCCCTTTC TGGTAGTACT GAATGTGAGT CCACCTGATC
  901 AGTGCAAAGC AAAGCTGTCA GCTAATATGA CATCAGTCGA TGAATATGTT GTCAGCTGAT
  961 GTCGTGATAA GCATCTGGAT CGTCATGGCC AGTCAACATC GCAAGTAACG GTCAGTTCAG
1021 AGATGCTCAT CGGATACACG TAGTCA
//
Primer: BGH_rev RDB12201_A4Fuc.seq
>12201_12201_A4Fu_BGHrev_C09_09.ab1
    1 GGTGTCTCAT GGTGATGGTG ATGATGACCG GTATGCATAT TCAGATCCTC TTCTGAGATG
   61 AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA CAGCGTATGA TGCGTATTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTCTTCCCCA AATTTTTTTT TGGGCCCTTT CATTTAAAAA AAAAAACTTT TACGGGTTTT
  241 TGGGGCCCCA AAACCAAGGG CCCCCCCCGG GGGGGAAATT CCGCAAAATT TCGGGGTTAC
  301 CCCACCCCCG GGGGGGACCC CTCGCCCCCC CCTTCCAAGC CCCCCCCCCC GGGGTCGGCC
  361 CCCCCGGGGG GCTTAGGGGG TTTCGGCCCC CGAAAGGAAA ACCCTTTTCC CCCAAAAACC
  421 CCGGGAAAGG GAAACCTAAA AAAGGGGGGC TTGGAATTTT TTTTGGGGGG GAAGGCCAGG
  481 GTGCCCCAGG CCTTGGCCCC TTCCCCGGGG GCCCCGGGCC GGGGGTTTTT TTCTCCCCCC
  541 GGGCAAAAAT TGGGGGTGCC CTGGGGGGGG TTAAGGGGGG GAACTAAGGG GCCAGGTTTT
  601 TTTCGAATTT CACGGAAAAT CCATTGGGTT TAAAAAACGG TTTGCCCTTG GGGTCTTTTG
  661 CCCTTCCGGC CCAGGGGGAA TAACCCGGAA ACAACCTCCG GAAGAGGTCT CGGGCCTCAG
  721 GCTAAACCCC CCTGGCGAGG ATTGCAGCGC GCCCCCCAGT TGAACCCCCC CACCCCCCCC
  781 CCTGTGGGGG GGGCCAAAAA GGGGGGTGCC CAACCGTATC TCAAAAAGAA TCCCCCCGCC
  841 CCCCCCCCAG TCACAGAGCT GCGTGTACCC TTTTTGCAGA GAAGCACCTC CGGAGCGTAT
  901 ATATTGTGTC TCACGACAAA AAGATGTGCC AGCACCTTGG GTCTGATTCG GCCCTTGGTC
961 CAGCCTTACG TCCCAGCGAG GTGACTCGTC AGTACCGCTC ATGCGTTCGT ATCGA
//
Primer: Lats2#11 RDB12201_A4Fud.seq
>12201_12201_A4Fu_Lats2#11_C02_08.ab1
    1 CAAAAGTCAG CCGGAGGCTA CAGCTGGAGC AGGAAATGGC CAAAGCTGGG CTCTGTGAGG
   61 CCGAGCAGGA GCAGATGAGG AAGATCCTCT ACCAGAAGGA GTCTAACTAC AACCGGCTGA
  121 AGAGGGCCAA GATGGACAAG TCCATGTTTG TGAAAATCAA GACTCTAGGC ATCGGTGCCT
  181 TTGGGGAAGT GTGCCTCGCT TGTAAGCTGG ACACTCACGC TCTGTACGCC ATGAAGACTC
  241 TCAGGAAGAA GGATGTCCTG AACCGGAATC AAGTGGCCCA TGTCAAGGCT GAGAGGGACA
  301 TCCTGGCTGA AGCAGACAAT GAGTGGGTGG TCAAACTCTA CTACTCCTTC CAGGACAAGG
  361 ACAGCCTGTA CTTTGTGATG GACTACATAC CAGGCGGGGA TATGATGAGC CTGCTGATCA
  421 GGATGGAGGT CTTCCCTGAG CACCTGGCCC GCTTCTACAT TGCAGAGTTG ACCCTGGCCA
  481 TTGAAAGTGT CCACAAGATG GGCTTTATCC ACCGGGACAT CAAGCCTGAC AACATACTCA
  541 TCGACCTGGA TGGTCATATT AAGCTGACAG CTTTTGGCCT CTGCACTGGA TTCAGGTGGA
  601 CTCACAATTC CAAGTACTAC CAGAAAGGGA ACCACATGAG ACAGGACAGC ATGGAGCCCG
  661 GTGACCTCTG GGACGATGTT TCCAACTGTC GCTGTGGAGA CAGGTTAAAG ACCCTGGAGC
  721 AGAGGGCGCA GAAGCAGCAC CAGAGGTGCC TGGCACATTC TCTTGTCGGG ACACCAAATT
  781 ACATCGCTCC GGAGGTGCTT CTCCGCAAAG GGTACACGCA GCTCTGTGAC TGGTGGAGCG
  841 TCGGTGTGAT TCTCTTTGAG ATGCTGGTTG GGCAGCCGCC TTTCTTGGCC CCCACCCCCC
  901 ACAGAGACGC AGCTGAATGG TGATCAACTG GGAGAGCACG CTGCATATCC CTACGCAGGT
  961 GAGGCTCAGC GCTGAGGCCC GAGACCTCAT CACGAAGCTG TGCTGCGCGG CTGACTGCCC
 1021 GCCCTGGCAG GATGGGGGCA GATGACCTCA AGGCACACCC GATTCTTCAA CACCATCGAA
1081 CTTTTCCGTT GACATCGGAA AGGCCAGTCA GCACTCCTTT ACGGATTCCC TCCCA
//
Primer: SV40pro_F RDB12201_A4Fue.seq
>12201_12201_A4Dl_SV40pro_F_G10_19.ab1
    1 CCCTGTCCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTTCTG AGCGGGACTC TGGGGTTTCG CGAAATGACC CGAACCCAAG
  961 CGAACGCCCA TCCTGCCCAT CACGAGATTT CCGATTCGAT CGGCCGCCGT TCTTATGAAA
1021 TGTTCGGCTT CGTATTCGAT TTTCCAGGAA CGATC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl