RDB12190 - pcDNA3.1-pA83-HA-Yap

Resource data sheet
Prev.
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pcDNA3.1-pA83-HA-Yap

Expression clone of mouse Yap1

Catalog number	RDB12190
Resource name	pcDNA3.1-pA83-HA-Yap
Clone info.	Expression clone of mouse Yap1. cDNA fragment 210 to 1,663 nt of NM_009534.3 (CDS, 207..1625) is cloned.
Comment	See Fig. 4A of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone	pcDNA3.1-pA83 (Plasmid)
Size of vector backbone	5.9 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Yap1
Depositor\|Developer	Sasaki, Hiroshi \|

Other clones in our bank

mouse Yap1

External Database
Mouse Yap1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

Ordering
Order form [Credit Card Payment] [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose [Word]
Please visit Information of Request for Distribution.[link]

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12190	pcDNA3.1-pA83-HA-Yap	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-HA-Yap was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12190).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12190_A3I9p1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: T7 pro,insert 5'( HA, Yap 5') Sequence file: RDB12190_A3I9f.seq
>12190_1_CMV_F_A10_01.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG 61 TCTATATAAG CAGAGCTCTC TGGNTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA 121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCGAGCTCGG 181 ATCCACTAGT CCAGTGTGGT GGAATTGTAA TACGACTCAC TATAGGGAGA CCCAAGCTTG 241 CCGCCACCAT GTACCCATAC GACGTTCCAG ATTACGCTGG TACCGACCCC GCGCAACAGC 301 CGCCGCCCCA GCCGGCCCCG CAAGGCCCCG CGCCGCCGTC CGTGTCTCCG GCCGGGACCC 361 CCGCGGCCCC GCCCGCACCC CCGGCCGGCC ACCAGGTCGT GCACGTCCGC GGGGACTCGG 421 AGACCGACTT GGAGGCGCTC TTCAATGCCG TCATGAACCC CAAGACGGCC AACGTGCCTC 481 AGACCGTGCC CATGCGGCTT CGCAAGCTGC CCGACTCCTT CTTCAAGCCG CCTGAGCCCA 541 AGTCCCACTC GAGACANGNN NGTACTGATG CAGGTACTGC GGGAGCTCTG ACTCCACAGC 601 ATGTTCGAGC TCACTCCTCT CCAGCCTCCC TGCAGCTGGG TGCCGTTTCT CCTGGGACAC 661 TCACAGCCNG TGGNNNTGTC TCTGGCCCTG CCGCTGCCCC TGCANCTCAN CATCTCCGGC 721 AGTCCTCCTT TGAGATCCCT GATGATGTAN CNACTGCCAG CAGNNNTGGG AGATGGCCNA 781 GACATCTTCT GGTCAAAGAN ACTTCTNANN TCACNNCGAT CANACNACAA CATGNNNNNG 841 NNCCCNNGAN NGNNNNGNNT TTCNNNNNCT GAACGTTCCT GCGCCNGNCC NNNCNAGCGG 901 NGCCCCNNNA NGCTGNNGAN NNNNNNCNNC NNNACCNNCN NNCCNGANNG NNGGNANNNN 961 GCNNNGNNNN NNNNNGGNNG NANNNTTANN TANNNNNNNC NNNAANAAAC NNNANCNCAT 1021 NNTNGNNTNN GNNNCCNANN NNNNGNNNCN NNCNTTTTNN CNNTNNNAAC CNNNNNNNNN 1081 N //
Primer: GNP Primer #10 (Pr0763) Region: insert mid(Yap 3') Sequence file: RDB12190_A3I9g.seq
>12190_1_GNP10_B08_05.ab1 1 NNNNNNNNNN NNNNNNCATT NNNNNNNNNA ACGTTTACNG GTTCTTTGGT GCCCATAGTC 61 GAGCGGCCGC CACTGTGCTG GATATCTGCA GAATTTAGAA GGCACAGTCG AGGCTGATCA 121 GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC TCTAGATGCA TGCTCGAGCG 181 GCCGCCAGTG TGATGGATAT CTGCAGAATT CCAGCACACT GGCGGCCGTT ACTAGTGGAT 241 CCCTCACAGA CTCAGAGTGG CTCCCTGCAG CTCTATAACC ACGTGAGAAA GCTTTCTTTA 301 TCTAGCTTGG TGGCAGCCAA CACAGACTCC ACGTCCAAGA TTTCGGAACT CAGCGCTTCC 361 TGCAGACTGG GCATCAGCTC CTCCCCTTCT ATGTTCATTG CATCTCCTTC CAGTGTGCCA 421 AGGTCCACAT TTGTCCCAGG GAGGGCTTCC AGGTANNCGG NNAANCGGCT CTGCTGTGAC 481 GGCAGGGTGC TTTGGCTGAT GGTGNNNCNN GNNNNNNTTT CNTCCNNNNT GNNGANGAAN 541 NCNTCTGNNN NNNNANNNAN NNNNNNNNTG NNCNNGNNGA NNNNNNTNNN NNNNNNN //
Primer: RDB15793_F1 (Pr0550) Region: insert 5'( HA, Yap 5') Sequence file: RDB12190_A3I9h.seq
>12190_12190_B3CfF_RDB15793_F1_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGCTAGNN NNNNTTGGTA CCGAGCTCGG ATCCACTAGT 61 CCAGTGTGGT GGAATTGTAA TACGACTCAC TATAGGGAGA CCCAAGCTTG CCGCCACCAT 121 GTACCCATAC GACGTTCCAG ATTACGCTGG TACCGACCCC GCGCAACAGC CGCCGCCCCA 181 GCCGGCCCCG CAAGGCCCCG CGCCGCCGTC CGTGTCTCCG GCCGGGACCC CCGCGGCCCC 241 GCCCGCACCC CCGGCCGGCC ACCAGGTCGT GCACGTCCGC GGGGACTCGG AGACCGACTT 301 GGAGGCGCTC TTCAATGCCG TCATGAACCC CAAGACGGCC AACGTGCCTC AGACCGTGCC 361 CATGCGGCTT CGCAAGCTGC CCGACTCCTT CTTCAAGCCG CCTGAGCCCA AGTCCCACTC 421 GAGACAGGCC AGTACTGATG CAGGTACTGC GGGAGCTCTG ACTCCACAGC ATGTTCGAGC 481 TCACTCCTCT CCAGCCTCCC TGCAGCTGGG TGCCGTTTCT CCTGGGACAN TCNCNNNCAG 541 TGGNNNNNNC TCTGGCCCTG CCGNNNNNCC TGCNNNTCAN NATCTCCGGN NNNCCTCCTT 601 TGANANNNNN NANNNNNNNN CACTGCCANC NNNGCTGGNN NNTGGNCCAN NANNTCNNNN 661 NNGNNNNNAN NNNANTNNNN NNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA, TRF2-3'UTR+polyA83 Sequence file: RDB12190_A3I9i.seq
>12190_12190_B0CjF_BGH_rev2_C02_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNG GNNGGGCNNA CAACAGANGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGGGTT TAAACTCAAT GGTGATGGTG ATGATGACCG GTATGCATAT 121 TCAGATCCTC TTCTGAGATG AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA 181 CAGCGTATGA TGCGTATTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 241 TTTTTTTTTT TTTTTTTTTT TTNNNNNNNN NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB12190_A3I9j.seq
>12190_12190_B0CjF_SV40pro_ori_F_E02_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNCCN CTGCCTCTGN NCTATTCCNG AAGTAGTGAG GAGGCTTTTT 61 TGGAGGCCTA NGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA 121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC 181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG 241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC 301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC 361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG 421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA 481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG 541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG 601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT 661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGATCTC GTCGTGACCC ATGGCGATGC 721 CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCNTCG ACNGTGGNCG 781 GCTGGGNGTG GCGGANCGCT ATCANGANNT ANCGTTGGCT ACCNNNGANA TNGNTGANNA 841 NCTTGGCGGC GAANGGGCTG ANCGCTTCCN CNNGCTTTAC GGTNTCGCNG CTNCNNATTC 901 NCANCNNATC GCNNNCNATC GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]
reference	Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415. [link to RRC of NBRP]

2024.03.26

HTML_filter_RDB_240326.pl