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pcDNA3.1-pA83-HA-Yap (#RDB12190)

Expression clone of mouse Yap1

Clone info. Expression clone of mouse Yap1. cDNA fragment 210 to 1,663 nt of NM_009534.3 (CDS, 207..1625) is cloned.
Comment See Fig. 4A of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12190 pcDNA3.1-pA83-HA-Yap DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-HA-Yap was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12190).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12190_A3I9p1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: T7 pro,insert 5'( HA, Yap 5')
Sequence file: RDB12190_A3I9f.seq check
>12190_1_CMV_F_A10_01.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCTC TGGNTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA
  121 TTAATACGAC TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCGAGCTCGG
  181 ATCCACTAGT CCAGTGTGGT GGAATTGTAA TACGACTCAC TATAGGGAGA CCCAAGCTTG
  241 CCGCCACCAT GTACCCATAC GACGTTCCAG ATTACGCTGG TACCGACCCC GCGCAACAGC
  301 CGCCGCCCCA GCCGGCCCCG CAAGGCCCCG CGCCGCCGTC CGTGTCTCCG GCCGGGACCC
  361 CCGCGGCCCC GCCCGCACCC CCGGCCGGCC ACCAGGTCGT GCACGTCCGC GGGGACTCGG
  421 AGACCGACTT GGAGGCGCTC TTCAATGCCG TCATGAACCC CAAGACGGCC AACGTGCCTC
  481 AGACCGTGCC CATGCGGCTT CGCAAGCTGC CCGACTCCTT CTTCAAGCCG CCTGAGCCCA
  541 AGTCCCACTC GAGACANGNN NGTACTGATG CAGGTACTGC GGGAGCTCTG ACTCCACAGC
  601 ATGTTCGAGC TCACTCCTCT CCAGCCTCCC TGCAGCTGGG TGCCGTTTCT CCTGGGACAC
  661 TCACAGCCNG TGGNNNTGTC TCTGGCCCTG CCGCTGCCCC TGCANCTCAN CATCTCCGGC
  721 AGTCCTCCTT TGAGATCCCT GATGATGTAN CNACTGCCAG CAGNNNTGGG AGATGGCCNA
  781 GACATCTTCT GGTCAAAGAN ACTTCTNANN TCACNNCGAT CANACNACAA CATGNNNNNG
  841 NNCCCNNGAN NGNNNNGNNT TTCNNNNNCT GAACGTTCCT GCGCCNGNCC NNNCNAGCGG
  901 NGCCCCNNNA NGCTGNNGAN NNNNNNCNNC NNNACCNNCN NNCCNGANNG NNGGNANNNN
  961 GCNNNGNNNN NNNNNGGNNG NANNNTTANN TANNNNNNNC NNNAANAAAC NNNANCNCAT
 1021 NNTNGNNTNN GNNNCCNANN NNNNGNNNCN NNCNTTTTNN CNNTNNNAAC CNNNNNNNNN
1081 N
//
Primer: GNP Primer #10 (Pr0763)
Region: insert mid(Yap 3')
Sequence file: RDB12190_A3I9g.seq check
>12190_1_GNP10_B08_05.ab1
    1 NNNNNNNNNN NNNNNNCATT NNNNNNNNNA ACGTTTACNG GTTCTTTGGT GCCCATAGTC
   61 GAGCGGCCGC CACTGTGCTG GATATCTGCA GAATTTAGAA GGCACAGTCG AGGCTGATCA
  121 GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC TCTAGATGCA TGCTCGAGCG
  181 GCCGCCAGTG TGATGGATAT CTGCAGAATT CCAGCACACT GGCGGCCGTT ACTAGTGGAT
  241 CCCTCACAGA CTCAGAGTGG CTCCCTGCAG CTCTATAACC ACGTGAGAAA GCTTTCTTTA
  301 TCTAGCTTGG TGGCAGCCAA CACAGACTCC ACGTCCAAGA TTTCGGAACT CAGCGCTTCC
  361 TGCAGACTGG GCATCAGCTC CTCCCCTTCT ATGTTCATTG CATCTCCTTC CAGTGTGCCA
  421 AGGTCCACAT TTGTCCCAGG GAGGGCTTCC AGGTANNCGG NNAANCGGCT CTGCTGTGAC
  481 GGCAGGGTGC TTTGGCTGAT GGTGNNNCNN GNNNNNNTTT CNTCCNNNNT GNNGANGAAN
541 NCNTCTGNNN NNNNANNNAN NNNNNNNNTG NNCNNGNNGA NNNNNNTNNN NNNNNNN
//
Primer: RDB15793_F1 (Pr0550)
Region: insert 5'( HA, Yap 5')
Sequence file: RDB12190_A3I9h.seq check
>12190_12190_B3CfF_RDB15793_F1_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGCTAGNN NNNNTTGGTA CCGAGCTCGG ATCCACTAGT
   61 CCAGTGTGGT GGAATTGTAA TACGACTCAC TATAGGGAGA CCCAAGCTTG CCGCCACCAT
  121 GTACCCATAC GACGTTCCAG ATTACGCTGG TACCGACCCC GCGCAACAGC CGCCGCCCCA
  181 GCCGGCCCCG CAAGGCCCCG CGCCGCCGTC CGTGTCTCCG GCCGGGACCC CCGCGGCCCC
  241 GCCCGCACCC CCGGCCGGCC ACCAGGTCGT GCACGTCCGC GGGGACTCGG AGACCGACTT
  301 GGAGGCGCTC TTCAATGCCG TCATGAACCC CAAGACGGCC AACGTGCCTC AGACCGTGCC
  361 CATGCGGCTT CGCAAGCTGC CCGACTCCTT CTTCAAGCCG CCTGAGCCCA AGTCCCACTC
  421 GAGACAGGCC AGTACTGATG CAGGTACTGC GGGAGCTCTG ACTCCACAGC ATGTTCGAGC
  481 TCACTCCTCT CCAGCCTCCC TGCAGCTGGG TGCCGTTTCT CCTGGGACAN TCNCNNNCAG
  541 TGGNNNNNNC TCTGGCCCTG CCGNNNNNCC TGCNNNTCAN NATCTCCGGN NNNCCTCCTT
  601 TGANANNNNN NANNNNNNNN CACTGCCANC NNNGCTGGNN NNTGGNCCAN NANNTCNNNN
661 NNGNNNNNAN NNNANTNNNN NNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGH pA, TRF2-3'UTR+polyA83
Sequence file: RDB12190_A3I9i.seq check
>12190_12190_B0CjF_BGH_rev2_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNNGGGCNNA CAACAGANGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACTCAAT GGTGATGGTG ATGATGACCG GTATGCATAT
  121 TCAGATCCTC TTCTGAGATG AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA
  181 CAGCGTATGA TGCGTATTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
241 TTTTTTTTTT TTTTTTTTTT TTNNNNNNNN NN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB12190_A3I9j.seq check
>12190_12190_B0CjF_SV40pro_ori_F_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNCCN CTGCCTCTGN NCTATTCCNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA NGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
  121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
  241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
  301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
  361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
  421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
  541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
  601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT
  661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGATCTC GTCGTGACCC ATGGCGATGC
  721 CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCNTCG ACNGTGGNCG
  781 GCTGGGNGTG GCGGANCGCT ATCANGANNT ANCGTTGGCT ACCNNNGANA TNGNTGANNA
  841 NCTTGGCGGC GAANGGGCTG ANCGCTTCCN CNNGCTTTAC GGTNTCGCNG CTNCNNATTC
901 NCANCNNATC GCNNNCNATC GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]
reference Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415. [link to RRC of NBRP]