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pcDNA3.1-pA83-HA-Yap

Expression clone of mouse Yap1

Catalog number RDB12190
Resource name pcDNA3.1-pA83-HA-Yap
Clone info. Expression clone of mouse Yap1. cDNA fragment 210 to 1,663 nt of NM_009534.3 (CDS, 207..1625) is cloned.
See Fig. 4A of Nishioka et al., Dev. Cell 16: 398-410 (2009). The expression vector has a stretch of poly(A). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Yap1 (NCBI Gene 22601) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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RDB12190 pcDNA3.1-pA83-HA-Yap DNA solution

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References and tips

Electronic file

Featured content

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12190_A4Dlp1_v2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12190_A4Dla.seq
>12190_12190_A4Dl_CMV-Forward_A04_01.ab1
    1 CTGGTAAACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTT GGTACCGAGC TCGGATCCAC
  181 TAGTCCAGTG TGGTGGAATT GTAATACGAC TCACTATAGG GAGACCCAAG CTTGCCGCCA
  241 CCATGTACCC ATACGACGTT CCAGATTACG CTGGTACCGA CCCCGCGCAA CAGCCGCCGC
  301 CCCAGCCGGC CCCGCAAGGC CCCGCGCCGC CGTCCGTGTC TCCGGCCGGG ACCCCCGCGG
  361 CCCCGCCCGC ACCCCCGGCC GGCCACCAGG TCGTGCACGT CCGCGGGGAC TCGGAGACCG
  421 ACTTGGAGGC GCTCTTCAAT GCCGTCATGA ACCCCAAGAC GGCCAACGTG CCTCAGACCG
  481 TGCCCATGCG GCTTCGCAAG CTGCCCGACT CCTTCTTCAA GCCGCCTGAG CCCAAGTCCC
  541 ACTCGAGACA GGCCAGTACT GATGCAGGTA CTGCGGGAGC TCTGACTCCA CAGCATGTTC
  601 GAGCTCACTC CTCTCCAGCC TCCCTGCAGC TGGGTGCCGT TTCTCCTGGG ACACTCACAG
  661 CCAGTGGCGT TGTCTCTGGC CCTGCCGCTG CCCCTGCAGC TCAGCATCTC CGGCAGTCCT
  721 CCTTTGAGAT CCCTGATGAT GTACCACTGC CAGCAGGCTG GGAGATGGCC AAGACATCTT
  781 CTGGTCAAAG ATACTTCTTA AATCACAACG ATCAGACAAC AACATGGCAG GACCCCCGGA
  841 AGGCCATGCT TTCGCAACTG AACGTTCCTG CGCCTGCCAG CCCAGCGGTG CCCCAGACGC
  901 TGATGAATTC TGCCTCACGA CCTCTTCCTG ATGGATGGGA GCAAGCCATG ACTCACGATG
  961 GAGAAGTTTA CTACATAAAC CATAAGAACA AGACCACATC CTGGCTGGGA CCCAAGGCTG
 1021 GGACCCTCGT TTTGCCATGA ACCCAGAGGG ATCACTCAGA GTTGCTCCAG TTGAGCAGCC
1081 CCCCCACCCG TGCTCCCAGA GCTCACAGGG AGGCGTCCTG GGTGGAGGCA AGATTTCA
//
Primer: GNP_primer#10 RDB12190_A4Dlb.seq
>12190_12190_A4Dl_GNP_primer+#10_B04_04.ab1
    1 TTATTTGTGC CATTCATTTA AAAGATAACG TTTACAGGTT CTTTGGTGCC CATAGTCGAG
   61 CGGCCGCCAC TGTGCTGGAT ATCTGCAGAA TTTAGAAGGC ACAGTCGAGG CTGATCAGCG
  121 AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT AGATGCATGC TCGAGCGGCC
  181 GCCAGTGTGA TGGATATCTG CAGAATTCCA GCACACTGGC GGCCGTTACT AGTGGATCCC
  241 TCACAGACTC AGAGTGGCTC CCTGCAGCTC TATAACCACG TGAGAAAGCT TTCTTTATCT
  301 AGCTTGGTGG CAGCCAACAC AGACTCCACG TCCAAGATTT CGGAACTCAG CGCTTCCTGC
  361 AGACTGGGCA TCAGCTCCTC CCCTTCTATG TTCATTGCAT CTCCTTCCAG TGTGCCAAGG
  421 TCCACATTTG TCCCAGGGAG GGCTTCCAGG TAGTCGGGGA AGCGGCTCTG CTGTGACGGC
  481 AGGGTGCTTT GGCTGATGGT GTCTCCTGTA TCCATTTCAT CCACACTGTT GAGGAAGTCG
  541 TCTGGGGTCC GAGGGATGCT GTAGCTGCTC ATGCTGAGGC CGCTGTCTGT GCTCTCATCT
  601 CGAGAGTGAT AGGTGCCACT GTTAAGAAAG GGATCGGAAC TATTGGTTGT CATTGTTCTC
  661 AATTCCTGAG ACATCCCAGG AGAAGACACT GCATTCGGAG TCCCTCCATC CTGCTCCAGT
  721 GTAGGCAACT GGCTGCGCAG AGCTAATTCC TGCCGAAATA ATTCCTGTTG TTTCAACCGC
  781 AGTCTCTCCT TCTCCATCTG TAACTGCTGC AGCTGTATTT GCTGCTGCTG GTTGGAACTG
  841 CCTCCACCCA GGACGCCTCC CTGTGGGCTC TGGGGAGCCA AGGGTGGGGG CTGCTTCACT
  901 GGAGCACTCT GAGTGATCCT CTGGTTCATG GCAAAACGAG GGTCCAGCCT TGGGTCCAGC
  961 CAGGATGTGG TCTTGTTCTT ATGGGTTTAT GTAGTAAACT TCTCCATCCT GAGTCATGGC
 1021 TTGCTCCCAT CCATCAGGAA GAGGTCCTGA GGCAGAATTT CATCAGCGTC TGGGGCACTC
 1081 GCTGGGCTGG CAAGCGCAAG CACCGATCCA GTGCGAAGGC ATTGCCTTCC GGGGGGGATT
1141 CCTGTTCT
//
Primer: f1ori_R3 RDB12190_A4Dlc.seq
>12190_12190_A4Dl_f1ori_R3_A01_01.ab1
    1 GGATGCATGA CCCAGCTGGT TCTTTCCGCC TCAGAAGCCA TAGAGCCCAC CGCATCCCCA
   61 GCATGCCTGC TATTGTCTTC CCAATCCTCC CCCTTGCTGT CCTGCCCCAC CCCACCCCCC
  121 AGAATAGAAT GACACCTACT CAGACAATGC GATGCAATTT CCTCATTTTA TTAGGAAAGG
  181 ACAGTGGGAG TGGCACCTTC CAGGGTCAAG GAAGGCACGG GGGAGGGGCA AACAACAGAT
  241 GGCTGGCAAC TAGAAGGCAC AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG
  301 TGATGATGAC CGGTATGCAT ATTCAGATCC TCTTCTGAGA TGAGTTTTTG TTCGAAGGGC
  361 CCTCTAGACT CGAGGTACGC CACAGCGTAT GATGCGTATT TTTTTTTTTT TTTTTTTTTT
  421 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTCC TTTCCCAAAT
  481 TTTTTTTTGG GCCCTTTTAT TTAAAAAAAA AAACTTTTAA GGGTTTTTGG GGGCCCAAAA
  541 CCAAGGGGCC CCCCTGGGGG GGGAATTTGG AAAAATTTAA AAGGCCCATC CAGGGGTAAT
  601 CCCCAACCTT TAATTTTTGG GGAACCCAAA AAAAAGGGGC CCCTAAAAAC CTTGCCCAAG
  661 GGCCCCCCAG GGGGAGGAAT TTTGGAAAAA TTCCCCCCCC GTGGGGGCCT TTTAAAGGGA
  721 TCCCCCCCAA AACCCAAGGG GGCTCCCGGG GTCTTTAAAA CCCGGGGAAA AATTTTTTTT
  781 TTTTTTTTTG GGGGGGGCCC AAAAAAAACC CCCCCCAAAA ATTTGGAAAC CCCCCCTTTC
  841 CTGAAAATGG GGAAAACCCC CCCCCCCTTT AAGTTTATGG CCCCCCCCCC AGGGGGGCAA
  901 GGGCCCATTT TTTTCCCAGG GGAGGGCTTT CCAGATATTC CGGGAACCCC CTCTTGTGTG
  961 TAAAGGCGGG GTCTTTTGGG CGAAAGGGGG CCCCCTGAAT ACTTTCTCCC CCACTGTGTT
 1021 GAGAATATCT CTCTGGGGGC TCAAGAGATA GTTAACTGCC TCATGCTAGA GCCCCCGTTT
 1081 GTGTTCTCTC TATCCAAAGA GTGAGAGGGC CCCCTGTTTA GGAGAGGATC TCGACACATT
1141 TGTTGTGCTA TGTTCTCATA TCCGCGGAGA GCACACTACT CCCACAGGAG
//
Primer: SV40pro_F RDB12190_A4Dld.seq
>12190_12190_A4Dl_SV40pro_F_B03_06.ab1
    1 GCTTGTCCCT GGGGCATTCA GAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGCGAAATGA CCGACCAAGC
  961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC CGCCTTCTAT GAAAGGTTGG
 1021 GCTTCGGAAT CGTTTTCCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG
 1081 CTGGAGTTCT TCGGCCCCAC CCCAACCTGT TAATGGCCAG CCTATAATGG TACAAATAAA
1141 GCAATTAGGC ATTCCACCAA AACTTC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.
reference Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415.

2019.04.09

GNP_filter3_RDBDEP_html_190327.pl