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8xGT-IIC-LucII

Reporter plasmid carring eight copies of Tead-binding motif

Catalog number RDB08067
Resource name 8xGT-IIC-LucII
Clone info. Reporter plasmid carring eight copies of Tead-binding motif.
Monitoring Hippo signaling. See Fig. 1G of Ota and Sasaki, Development 135: 4059-4069 (2008). Use delta51-LucII (cat.# RDB08068) as a negative control.
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor Sasaki, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Development, 135, 4059-4069 (2008).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08067 8xGT-IIC-LucII DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB08067.pdf from Depositor

Featured content

Featured content Hippo signaling (English text)

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08067_A3Hr.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGV-CCW RDB08067_A3Hra.seq
>8067-2-CCW_D10_10.ab1
    1 GTGCCCCGTT CGGAGATGCC AGGCTTGGCT GCCCGCACAG CCCTGCTCAC CGTCTCCGCA
   61 GCTCAGCCCT GGCTGGTCGC TCCGTGCTCT CCGGTCTCAC AGCCCCGCTT TTACGTGCCC
  121 CGGAGCCTGG GCTGGCCCTG CAGGTCGACG GTATCGATAA GCTTGATGGA CACACATTCC
  181 ACAGCTGGGG ACACACATTC CACAGCTGGG GACACACATT CCACAGCTGG GGACACACAT
  241 TCCACAGCTG GGGACACACA TTCCACAGCT GGGGACACAC ATTCCACAGC TGGGGACACA
  301 CATTCCACAG CTGGGGACAC ACATTCCACA GCATCGAATT CCTGCAGCCC GGGGGATCCA
  361 GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA
  421 TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT
  481 AAACAAGTTA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG
  541 GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGATATGG CTGATCCAGA CATGATAAGA
  601 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
  661 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
  721 AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC
  781 AAGTAAAACC TCTACAAATG TGATATGGCT GATCCTGCCT CGCGCGTTTC GGTGATGACG
  841 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG
  901 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGGTG TTGGCGGGTG TCGGGGCGCA
  961 GCCATGACCC CCTGCATTAA TGAATCGGCC AACCGCGCGG GGAGAGGCGG TTTGCGTTAT
 1021 TGGGCGCTCT TCCGCTTTCC TTCGCTCACT GACTTCGCTT GCGCTCGGTC GTTTCGGCCT
 1081 GCTGCCGAGC CGGTAATCAG CTCCACTTCA AGGCCGTTAA TTACTGTTAT CCCACAGAAA
1141 TTCCAGGGGG AATATAACAC GGTCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856.
reference Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852.
reference Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415.

2018.12.10

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