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8xGT-IIC-LucII

Reporter plasmid carring eight copies of Tead-binding motif

Catalog number RDB08067
Resource name 8xGT-IIC-LucII
Clone info. Reporter plasmid carring eight copies of Tead-binding motif.
Monitoring Hippo signaling. See Fig. 1G of Ota and Sasaki, Development 135: 4059-4069 (2008). Use delta51-LucII (cat.# RDB08068) as a negative control.
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 135, 4059-4069 (2008).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08067 8xGT-IIC-LucII DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Remarks, protocol and/or map (pdf) RDB08067.pdf

Original reference

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Hippo signaling (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08067_A3Hrp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: PGV-CCW
Region: Chicken gamma-crystallin Basal Pro,8XGT-Iic,SV40 pA
Sequence file: RDB08067_A3Hra.seq check
>08067_08067_A3Hr_pGV-CCW_C01_07.ab1
    1 GGCCGTCGGG ATGCCAGCTT GGCTGCCCGC ACAGCCCTGC TCACCGTCTC CGCAGCTCAG
   61 CCCTGGCTGG TCGCTCCGTG CTCTCCGGTC TCACAGCCCC GCTTTTACGT GCCCCGGAGC
  121 CTGGGCTGGC CCTGCAGGTC GACGGTATCG ATAAGCTTGA TGGACACACA TTCCACAGCT
  181 GGGGACACAC ATTCCACAGC TGGGGACACA CATTCCACAG CTGGGGACAC ACATTCCACA
  241 GCTGGGGACA CACATTCCAC AGCTGGGGAC ACACATTCCA CAGCTGGGGA CACACATTCC
  301 ACAGCTGGGG ACACACATTC CACAGCATCG AATTCCTGCA GCCCGGGGGA TCCAGACATG
  361 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT
  421 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA
  481 GTTAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT
  541 TAAAGCAAGT AAAACCTCTA CAAATGTGAT ATGGCTGATC CAGACATGAT AAGATACATT
  601 GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT
  661 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAT
  721 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
  781 AACCTCTACA AATGTGATAT GGCTGATCCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA
  841 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA
  901 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA
  961 CCCCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT
 1021 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTT CGGCTGCGGC GAGCGGTATC
 1081 AGCTCACTCA AAGGCGTATT ACCGGCTATC ACAGAATCAT GGGGAATAAC CGCCAGGAAA
1141 AAGAAAAACC AATGTGA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856.
reference Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852.
reference Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415.

2020.12.17

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