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8xGT-IIC-LucII

Reporter plasmid carring eight copies of Tead-binding motif

Catalog number RDB08067
Resource name 8xGT-IIC-LucII
Clone info. Reporter plasmid carring eight copies of Tead-binding motif.
Comment Monitoring Hippo signaling. See Fig. 1G of Ota and Sasaki, Development 135: 4059-4069 (2008). Use delta51-LucII (cat.# RDB08068) as a negative control.
Vector backbone delta51-LucII (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
firefly (Photinus pyralis) luciferase

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 135, 4059-4069 (2008).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 135, 4059-4069 (2008).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
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            RDB08067 8xGT-IIC-LucII DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (pdf) RDB08067.pdf

            Original reference

            original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Hippo signaling (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB08067_A3Hrp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: PGV-CCW
            Region: Chicken gamma-crystallin Basal Pro,8XGT-Iic,SV40 pA
            Sequence file: RDB08067_A3Hra.seq check
            >08067_08067_A3Hr_pGV-CCW_C01_07.ab1
                1 GGCCGTCGGG ATGCCAGCTT GGCTGCCCGC ACAGCCCTGC TCACCGTCTC CGCAGCTCAG
               61 CCCTGGCTGG TCGCTCCGTG CTCTCCGGTC TCACAGCCCC GCTTTTACGT GCCCCGGAGC
              121 CTGGGCTGGC CCTGCAGGTC GACGGTATCG ATAAGCTTGA TGGACACACA TTCCACAGCT
              181 GGGGACACAC ATTCCACAGC TGGGGACACA CATTCCACAG CTGGGGACAC ACATTCCACA
              241 GCTGGGGACA CACATTCCAC AGCTGGGGAC ACACATTCCA CAGCTGGGGA CACACATTCC
              301 ACAGCTGGGG ACACACATTC CACAGCATCG AATTCCTGCA GCCCGGGGGA TCCAGACATG
              361 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT
              421 ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA
              481 GTTAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT
              541 TAAAGCAAGT AAAACCTCTA CAAATGTGAT ATGGCTGATC CAGACATGAT AAGATACATT
              601 GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT
              661 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAT
              721 TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA
              781 AACCTCTACA AATGTGATAT GGCTGATCCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA
              841 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA
              901 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA
              961 CCCCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT
             1021 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTT CGGCTGCGGC GAGCGGTATC
             1081 AGCTCACTCA AAGGCGTATT ACCGGCTATC ACAGAATCAT GGGGAATAAC CGCCAGGAAA
            1141 AAGAAAAACC AATGTGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856.
            reference Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852.
            reference Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415.

            2022.05.17

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