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8xGT-IIC-LucII (#RDB08067)

Reporter plasmid carring eight copies of Tead-binding motif

Clone info. Reporter plasmid carring eight copies of Tead-binding motif.
Comment Monitoring Hippo signaling. See Fig. 1G of Ota and Sasaki, Development 135: 4059-4069 (2008). Use delta51-LucII (cat.# RDB08068) as a negative control.
Vector backbone delta51-LucII (RDB08068) (Plasmid)
Size of vector backbone 6.1 kb
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Sasaki, Hiroshi |
 
Remarks, protocol and/or map (pdf) RDB08067.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 135, 4059-4069 (2008).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 135, 4059-4069 (2008).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08067 8xGT-IIC-LucII DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The 8xGT-IIC-LucII was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08067).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19897_B3Bap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Luc-B_R (Pr0790)
Region: Luc 5',Chicken delta-crystallin Basal pro,8xGT-IIC,SV40 pA
Sequence file: RDB19897_B3Baa.seq check
>08067_19897_B3Ba_3_Luc-B_R_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCTCTTN NTAGCCTTAT GCNGTTGCTC TCCNGCGGTT
   61 CCNTCCTCTA GAGGANAGAA TGGCGCCGGG CCTTTCTTTA TGTTTTTGGC GTCTTCCATT
  121 TTACCAACAG TACCGGAATG CCAAGCTTGG CTGCCCGCAC AGCCCTGCTC ACCGTCTCCG
  181 CAGCTCAGCC CTGGCTGGTC GCTCCGTGCT CTCCGGTCTC ACAGCCCCGC TTTTACGTGC
  241 CCCGGAGCCT GGGCTGGCCC TGCAGGTCGA CGGTATCGAT AAGCTTGATG GACACACATT
  301 CCACAGCTGG GGACACACAT TCCACAGCTG GGGACACACA TTCCACAGCT GGGGACACAC
  361 ATTCCACAGC TGGGGACACA CATTCCACAG CTGGGGACAC ACATTCCACA GCTGGGGACA
  421 CACATTCCAC AGCTGGGGAC ACACATTCCA CAGCATCGAA TTCCTGCAGC CCGGGGGATC
  481 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA
  541 AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA
  601 ATAAACAAGT TAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GANGTGTGGG
  661 ANGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGATAT GGCTGATCCA GACATGATAA
  721 GATACATTGA TGANTTTGGA CAAACCACAA CTAGAATGNA GNGAAAAAAA TGCTTTATTT
  781 GTGAAANTTG TGANGCTATT GCTTTATTTG NAACCNTTAT AAGCTGCAAT AAACNAGTTN
  841 NCNACNNNNG CATNCATTTT ATGNTTCNNN NCNNNGGNNN NNGNNGNNNG NTTTTTTNAN
901 NCNNGTAAAN NNNNNNNNN
//
Primer: Luc_inner_R2 (Pr1286)
Region: Luc 5',Chicken delta-crystallin Basal pro
Sequence file: RDB19897_B3Bab.seq check
>08067_19897_B3Ba_3_Luc_inner_R2_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC TGNNGCANGC AGTTCTATGC GGAAGGGCCA CACCCTTAGG
   61 TAACCCAGTA GATCCAGAGG AATTCATTAT CAGTGCAATT GTTTTGTCAC GATCAAAGGA
  121 CTCTGGTACA AAATCGTATT CATTAAAACC GGGAGGTAGA TGAGATGTGA CGAACGTGTA
  181 CATCGACTGA AATCCCTGGT AATCCGTTTT AGAATCCATG ATAATAATTT TCTGGATTAT
  241 TGGTAATTTT TTTTGCACGT TCAAAATTTT TTGCAACCCC TTTTTGGAAA CAAACACTAC
  301 GGTAGGCTGC GAAATGTTCA TACTGTTGAG CAATTCACGT TCATTATAAA TGTCGTTCGC
  361 GGGCGCAACT GCAACTCCGA TAAATAACGC GCCCAACACC GGCATAAAGA ATTGAAGAGA
  421 GTTTTCACTG CATACGACGA TTCTGTGATT TGTATTCAGC CCATATCGTT TCATAGCTTC
  481 TGCCAACCGA ACGGACATTT CGAAGTATTC CGCGTACGTG ATGTTCACCT CGATATGTGC
  541 ATCTGTAAAA GCAATTGTTC CAGGAACCAG GGCGTATCTC TTCATAGCCT TATGCAGTTG
  601 CTCTCCAGCG GTTCCATCCT CTAGAGGATA GAATGGCGCC GGGCCTTTCT TTATGTTTTT
  661 GGCGTCTTCC ATTTTACCAA CAGTACCGGA ATGCCAAGCT TGGCTGCCCG CACAGCCCTG
  721 CTCACCGTCT CCGCAGCTCA GCCCTGGCTG GTCGCTCCGT GCTCTCCGGT CTCACAGCCC
  781 CGCTTTTACG TGCCCCGGAN CCTGGGCTGG CCCTGCANGT CGACGGTATC GATAAGCTTG
  841 ATGGNNACAC ATTCCACAGC NGGGGANACA CATTCCACAG CNNGGGNNAC ACATTCCACA
  901 NNNNGGGNNN ACACNTTCCN CANCNGGGGN NNNCNCNTNN CACAGCNNGG NNNNACACNN
961 TNNNNANNNN GGNNNNNNNN NTNNCNNNNN NNNGGNNNNN NNNNN
//
Primer: Luc inner_F1 (Pr0617)
Region: Luc mid
Sequence file: RDB19897_B3Bac.seq check
>08067_19897_B3Ba_3_Luc_inner_F1_D03_12_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTTG NNNNNGAGTC CTTTGNNCGT GACAAAACAA TTGCACTGAT
   61 AATGAATTCC TCTGGATCTA CTGGGTTACC TAAGGGTGTG GCCCTTCCGC ATAGAACTGC
  121 CTGCGTCAGA TTCTCGCATG CCAGAGATCC TATTTTTGGC AATCAAATCA TTCCGGATAC
  181 TGCGATTTTA AGTGTTGTTC CATTCCATCA CGGTTTTGGA ATGTTTACTA CACTCGGATA
  241 TTTGATATGT GGATTTCGAG TCGTCTTAAT GTATAGATTT GAAGAAGAGC TGTTTTTACG
  301 ATCCCTTCAG GATTACAAAA TTCAAAGTGC GTTGCTAGTA CCAACCCTAT TTTCATTCTT
  361 CGCCAAAAGC ACTCTGATTG ACAAATACGA TTTATCTAAT TTACACGAAA TTGCTTCTGG
  421 GGGCGCACCT CTTTCGAAAG AAGTCGGGGA AGCGGTTGCA AAACGCTTCC ATCTTCCAGG
  481 GATACGACAA GGATATGGGC TCACTGAGAC TACATCAGCT ATTCTGATTA CACCCGAGGG
  541 GGATGATAAA CCGGGCGCGG TCGGTAAAGT TGTTCCATTT TTTGAAGCGA AGGTTGTGGA
  601 TCTGGATACC GGGAAAACGC TGGGCGTTAA TCAGAGAGGC GAATTATGTG TCAGAGGANC
  661 TATGATTATG TCCGGTTATG TAAACAATCC GGAAGCGANC AACGCCTTGN TTGACAAGGN
  721 TGGATGGCTA CNTTCTGGAG ACATAGCTTA CTGGGNCGAA GANGAACACT TCTTCATAGT
  781 TGANNGCTTG AAGTCTTTAA TTNAATACNA AGGANNTCNN NTGGCCCCCG CTGNANTNNG
  841 NANTCGATNT TGTTACNACA CCCCNACATC TTCNANNCNG NNGNNNNNNN TCTTCCNNNN
901 NANGNNNNNN GNNNNNNNNC NNNNNNNNNN
//
Primer: pMAMneo-Reverse (Pr0088)
Region: Luc 3'
Sequence file: RDB19897_B3Bad.seq check
>08067_19897_B3Ba_3_pMAMneo-Reverse_C03_09_ABI24.ab1
    1 NNNNNNNNNN GNNNNNCNNN NNNAGTAAGG NTCCTTCNCN AANNANCCTC TAGAGTATTA
   61 CAATAGCTAA GAATTTCGTC ATCGCTGAAT ACAGTTACAT TTTACAATTT GGACTTTCCG
  121 CCCTTCTTGG CCTTTATGAG GATCTCTCTG ATTTTTCTTG CGTCGAGTTT TCCGGTAAGA
  181 CCTTTCGGTA CTTCGTCCAC AAACACAACT CCTCCGCGCA ACTTTTTCGC GGTTGTTACT
  241 TGACTGGCGA CGTAATCCAC GATCTCTTTT TCCGTCATCG TCTTTCCGTG CTCCAAAACA
  301 ACAACGGCGG CGGGAAGTTC ACCGGCGTCA TCGTCGGGAA GACCTGCCAC GCCCGCGTCG
  361 AAGATGTTGG GGTGTTGTAA CAATATCGAT TCCAATTCAG CGGGGGCCAC CTGATATCCT
  421 TTGTATTTAA TTAAAGACTT CAAGCGGTCA ACTATGAAGA AGTGTTCGTC TTCGTCCCAG
  481 TAAGCTATGT CTCCAGAATG TAGCCATCCA TCCTTGTCAA TCAAGGCGTT GGTCGCTTCC
  541 GGATTGTTTA CATAACCGGA CATAATCATA GGTCCTCTGA CACATAATTC GCCTCTCTGA
  601 TTAACGCCCA GCGTTTTCCC GGTATCCAGA TCCACAACCT TCGCTTCAAA AAATGGAACA
  661 ACTTTACCGA CCGCGCCCGG TTTATCATCC CCCTCGGGTG TAATCAGAAT AGCTGANGTA
  721 GTCTCAGTGA GCCCATATCC TTGTCGNATC CCTGGAAGAN GGAAGCGTTT TGCAACCGCT
  781 TCCCCGANTT CTTTCGAAAG ANGTGCGCCC CCNGAAGCAA TTTCGTGNNA ATTAGANNAA
  841 TCGTATTTGN CNNNCANANN GCTTTTGGNN AANAATGNAA ATNGGGNNGG NACTANCNNC
901 GCACTTNNNA NTTTNNNAAN NCNNGNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]
reference Nagashima, S., Validation of chemical compound library screening for transcriptional co-activator with PDZ-binding motif inhibitors using GFP-fused transcriptional co-activator with PDZ-binding motif. Cancer Sci. 107 (6): 791-802 (2016). PMID 27009852. [link to RRC of NBRP]
reference Tsutsumi, R., YAP and TAZ, Hippo signaling targets, act as a rheostat for nuclear SHP2 function. Dev. Cell, 26 (6): 658-665 (2013). PMID 24035415. [link to RRC of NBRP]