RDB12176 - pMYs-Tead4-VP16-IRES-EGFP

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pMYs-Tead4-VP16-IRES-EGFP (#RDB12176)

Retroviral vector of mouse Tead4, constitutive active

Clone info.	Retroviral vector of mouse Tead4, activation domain of herpes simplex virus VP16, constitutive active.
Comment	Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: C to G substitution (Pro, synonym) at 1265 nt of NM_011567.2.
Vector backbone	pMYs-IRES-EGFP (Plasmid)
Size of vector backbone	6.7 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Tead4 cDNA HSV VP16 protein cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.

Remarks Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks	Remember that you will be working with samples containing infections virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 MTAに書く付加的使用条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12176	pMYs-Tead4-VP16-IRES-EGFP	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMYs-Tead4-VP16-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12176).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12176_A6Ajp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro(pFB-5') () Sequence file: RDB12176_A6Aja.seq
>12176_12176_A6Aj_5'Retro(pFB-5')_E08_14.ab1 1 TTCAGCTAAG ATTCATCTCG GACTGCTAGT TATTAAGGAT CCCAGTGTGG TGGTACGGGA 61 ATTCCTGCAG GGTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT 121 AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 181 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGTACCAGG ATCCAGTGTG 241 GTGGAATTCA TTACCTCCAA CGAGTGGAGC TCTCCCGACT CCCCCGAGGG GAGCAGCATC 301 TCTGGGGGCA GCCAGGCACT GGACAAGCCC ATCGACAATG ATGCAGAGGG TGTATGGAGC 361 CCCGAAATTG AGCGAAGCTT CCAGGAGGCC CTGGCCATCT ACCCACCCTG CGGCCGCCGC 421 AAAATTATCC TGACGGAGGA AGGCAAGATG TATGGTCGGA ATGAGCTGAT CGCACGCCAT 481 ATCAAGCTCA GGACAGGGAA GACGCGCACA AGGAAGCAGG TCTCCAGCCA CATCCAGGTG 541 CTTGCCCGTC GAAAAGCCCG GGAGATCCAG GCCAAACTCA AGGACCAGGC AGCTAAGAAC 601 AAGGCCCTGC AGAGCATGGC TGCCATGTCG TCCGCACAGA TCGTCTCGGC CACAGCCTTC 661 CACAGTAAAA TGGCTCTTGC CCGGGGCCCT GGCTACCCAG CAATCTCAGG GTTTTGGCAA 721 GGAGCTTTGC CAGGCCAACC TGGAACATCC CACGATGTGA AACCTTTCTC TCAAAACACC 781 TACCCTGTCC AGCCTCCGCT GCCTCTGCCA GGCTTTGAGT CTCCTGCAGG GCCCACCCCC 841 TCACCCTCCG CACCGCTCGC TCCTCCGTGG CAAGGCCGCA GCATAGCTAG CTCCAAGCTC 901 TGGATGTTGG AGTTCTCGGC TTTCCTGGAG CGCCAGCAAG ATCCGGGACA CATACAACAA 961 ACACCTGTTT GTGCACATCA GCCCAGTCAA GCCCAAAGCT ACAGTGACCC CTACCTCGAA 1021 CTGTGGACAT CCCGCCCAAT CTATGACAGT TCCCAGAAGA GAATGGAGCC TCACAAGCTA 1081 TTTGACCGGG TCCTCTATGC TTTCTTCTGT GAAGGTCTGG CAGACCCCTC CA //
Primer: IRES-R () Sequence file: RDB12176_A6Ajb.seq
>12176_12176_A6Aj_IRES-R_D08_11.ab1 1 GCACGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGGGCGGAAT 61 TTACGTAGCG GCCGCGGCGC GCCGGCCCTC GAGGTAGAAG GCACAGTCGA GGCTGATCAG 121 CGGGTTTAAA CGGGCCCTCT AGATCCTACC CACCGTACTC GTCAATTCCA AGGGCATCGG 181 TAAACATCTG CTCAAACTCG AAGTCGGCCA TATCCAGAGC GCCGTAGGGG GCGGAGTCGT 241 GGGGGGTAAA TCCCGGACCC GGGGAATCCC CGTCCCCCAA CATGTCCAGA TCGAAATCGT 301 CTAGCGCGTC GGCATGCGCC ATCGCCACGT CCTCGCCGTC TAAGTGGAGC TCGTCCCCCA 361 GGCTGACATC GGTCGGGGGG GCATCGATGT TGGTATTGAG GTCTGCCCAG AACTTCACAA 421 GGAAGAAGGC ATTAGAGGGC CCCCGTTCAA ATAGCTCCTT GAGGCCTCCC TTCTTCTCTG 481 GGAACTTGTC ATAGATTTGG CGGATGTCCA CAGTTTCGAG GTAGGGGTCA CTGTAGCTTG 541 GGCTTGACTG GCTGATGTGC ACAAACAGGT GTTTGTTGTA TGTGTCCGGA TCTTGCTGGC 601 GCTCCAGGAA AGCCGAGAAC TCCAACATCC AGAGCTTGGA GCTAGCTATG CTGCGGCCTT 661 GCCACGGAGG AGCGAGCGGT GCGGAGGGTG AGGGGGTGGG CCCTGCAGGA GACTCAAAGC 721 CTGGCAGAGG CAGCGGAGGC TGGACAGGGT AGGTGTTTTG AGAGAAAGGT TTCACATCGT 781 GGGATGTTCC AGGTTGGCCT GGCAAAGCTC CTTGCCAAAA CCCTGAGATT GCTGGGTAGC 841 CAGGGCCCCG GGCAAGAGCC ATTTTACTGT GGAAGGCTGT GGCCGAGACG ATCTGTGCGG 901 ACGACATGGC AGCCATGCTC TGCAGGGCCT TGTTCTTAGC TGCCTGGTCC CTTGAGTTTG 961 GCCTGGATCT CCCGGGCTTT TCGACGGGCA AGCACCTGGA TGTGGCTGGG AGACCTGCTT 1021 CCTTTGTGCG CGTCTTTCCC TGTCCTGAGC TTTGATATGG GCGTGGCGAT CAGCTCATTC 1081 GACCATAACA TCTTGCCGTC CTCGTCAAGA ATAATTTGCC GCGCGGCAGG GATGGGATGA 1141 TGCCAGGACT CTGGAAGACC TTTTCG //
Primer: IRES-F () Sequence file: RDB12176_A6Ajc.seq
>12176_12176_A6Aj_IRES-F_F08_17.ab1 1 TCCAATCCGT TTCTGTGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC GAACCACGGG 61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG 121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG 181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT 241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT 301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT 361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG 421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA 481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA 541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA 601 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA 661 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA 721 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT 781 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC 841 AGCACAGTGG TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGG CCCGCGATTA 901 GTCCAATTTG TTAAAGACAG GATATCAGTG GTCCAGGCTC TAGTTTTGAC TCAACAATAT 961 CACCAGCTGA AGCCTATAGA GTACGAGCCA TAGATAGAAT AAAAGATTTT ATTTAGTCTC 1021 AGAAAAAGGG GGGGAAATGA AGACCCCACT GTAGTTTGGG CAAGCTTAGC TAGTACGCCA 1081 TTTTGCAAGC ATGGTAAAAT ACATAACCTG AGATAGAGAA GTTCAGGAAT TCAAGGCTTT 1141 AG //
Primer: Amp_R () Sequence file: RDB12176_A6Ajd.seq
>12176_12176_A6Aj_Amp_R_G08_20.ab1 1 TTCGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 121 CTGCATAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG 181 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAAAAGTTC AGATCAAGGT CAGGAACAGA 241 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC 301 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG 361 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA 421 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC 481 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG 541 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGACTGAGT 601 CGCCCGGGTA CCCGTATTCC CAATAAAGCC TCTTGCTGTT TGCATCCGAA TCGTGGACTC 661 GCTGATCCTT GGGAGGGTCT CCTCAGATTG ATTGACTGCC CACCTCGGGG GTCTTTCATT 721 TGGAGGTTCC ACCGAGATTT GGAGACCCCT GCCCAGGGAC CACCGACCCC CCCGCCGGGA 781 GGTAAGCTGG CCAGCGGTCG TTTCGTGTCT GTCTCTGTCT TTGTGCGTGT TTGTGCCGGC 841 ATCTAATGTT TGCGCCTGCG TCTGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC 901 CGTGGTGGAA CTGACGAGTT CGGAACACCC GGGCCGCAAC CCTGGAGACG TCCAGGACTT 961 CGGGGGCCGT TTTGTGCCCG ACTGAGTCAA AATCCGATCG GTTTGACTCT TGTGCACCCC 1021 CCTTATAGGA AATTGTGATC TGTTAGAGAC GAACCTAAAC AGTTCGCTTC GACTGATTAG 1081 CATCGATGGA CGAGCGGCGG GCGACCTGAC T //
Primer: pMFG-1 () Sequence file: RDB12176_A6Aje.seq
>12176_12176_A6Aj_pMFG-1_H08_23.ab1 1 AAAAGGGGGG GAAGGGCTGT TAGTAACTCT TGTCATGTCA GGGTCAGGGA AGTTTACAAG 61 GGGCGGGGGT GCCCCATATA AGATCTCATA TGGCCATATG GGGGCGCCTA GAGAAGGAGT 121 GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA GGGGGAGAGA 181 CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT TGACCCAGGG 241 AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT CTGGGTGTCC 301 ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC GGTTAAAGGT 361 GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA AGGTAACCCA 421 ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT CTTTCCAGTG 481 ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG ACAAATACAG 541 AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CAGACAAGAC GCGCGGCGCG 601 GCTTCGGTCC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT TTAGGTTCTC 661 GTCTCCTACC AGAACCACAT ATCCCTCCTA TTAGGGGGGT GCACCAAAGA GTCCAAAACG 721 ATCGGGATTT TTGGACTCAG GTCGGGCCAC AAAAACGGCC CCCGAAGTCC CTGGGACGTC 781 TCCCAGGGTT GCGGCCGGGT GTTCCGAACT CGTCAGTTCC ACCACGGGTC CGCCAGATAC 841 AGAGCTAGTT AGCTAACTAG TACAGACGCA GGCGCAAACA TTAGATGCCG GCACAAACAC 901 GCACAAAGAC AGAGACAGAC ACGAAACGAC CGCTGGGCCA GCTTACCTCC CGGCGGGGGG 961 GGTCGGTGGT CCCTGGGCAG GGGTCTCCAA ATCTCGGTGG AACCCTCCAA ATGAAAGACC 1021 CCCGGAGTGG GCAGTCAATC AATCTGAAGG AGACCCCTCC CAAAGAATCA GCGAAGTCCA 1081 CGATCGATGC AAACAGCCAA GAAGGCCTTT ATGGAAATAC GGGTACCGGG CGACTCAGTT 1141 CAATTCGAGA CTGCCGCCGA ATTAGAGGTA //
Primer: EGFP-C () Sequence file: RDB12176_A6Ajf.seq
>12176_12176_A6Aj_EGFP-C_A09_03.ab1 1 CAGGGGAATC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG 61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT 121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA 181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG 241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA 301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA 361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC 421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT 481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC 541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT 601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC 661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC 721 GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT 781 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGGTCT TTCACATGCA 841 GCATGTATCA AAATTAATTT GGTTTTTTTT CTTAAGTATT TACATTAAAT GGCCATAGTT 901 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 961 TTCCTCGCTC ACTGACTCGC TGCGCTCGGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 1021 ACTCAAAGGC GTAATACGGT TATCCACAGA AATCAGGGGA TACGCAGAAA GAACATGTGG 1081 AGCCAAGGCA GCAACGCCAA GGAACCGTAA AAGGCCCGCG ATTTACCTTT G //

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References

Original, user report and related articles

original	Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]