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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMYs-Tead4-VP16-IRES-EGFP

Retroviral vector of mouse Tead4, constitutive active

Catalog number RDB12176
Resource name pMYs-Tead4-VP16-IRES-EGFP
Clone info. Retroviral vector of mouse Tead4, activation domain of herpes simplex virus VP16, constitutive active.
Comment Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: C to G substitution (Pro, synonym) at 1265 nt of NM_011567.2.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead4 cDNA HSV VP16 protein cDNA
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank

External Database
Mouse Tead4 HSV VP16 protein

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infections virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          付加的提供条件:
          pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12176 pMYs-Tead4-VP16-IRES-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12176_A6Ajp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: 5'Retro(pFB-5') ()
          Sequence file: RDB12176_A6Aja.seq check
          >12176_12176_A6Aj_5'Retro(pFB-5')_E08_14.ab1
              1 TTCAGCTAAG ATTCATCTCG GACTGCTAGT TATTAAGGAT CCCAGTGTGG TGGTACGGGA
             61 ATTCCTGCAG GGTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT
            121 AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT
            181 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGTACCAGG ATCCAGTGTG
            241 GTGGAATTCA TTACCTCCAA CGAGTGGAGC TCTCCCGACT CCCCCGAGGG GAGCAGCATC
            301 TCTGGGGGCA GCCAGGCACT GGACAAGCCC ATCGACAATG ATGCAGAGGG TGTATGGAGC
            361 CCCGAAATTG AGCGAAGCTT CCAGGAGGCC CTGGCCATCT ACCCACCCTG CGGCCGCCGC
            421 AAAATTATCC TGACGGAGGA AGGCAAGATG TATGGTCGGA ATGAGCTGAT CGCACGCCAT
            481 ATCAAGCTCA GGACAGGGAA GACGCGCACA AGGAAGCAGG TCTCCAGCCA CATCCAGGTG
            541 CTTGCCCGTC GAAAAGCCCG GGAGATCCAG GCCAAACTCA AGGACCAGGC AGCTAAGAAC
            601 AAGGCCCTGC AGAGCATGGC TGCCATGTCG TCCGCACAGA TCGTCTCGGC CACAGCCTTC
            661 CACAGTAAAA TGGCTCTTGC CCGGGGCCCT GGCTACCCAG CAATCTCAGG GTTTTGGCAA
            721 GGAGCTTTGC CAGGCCAACC TGGAACATCC CACGATGTGA AACCTTTCTC TCAAAACACC
            781 TACCCTGTCC AGCCTCCGCT GCCTCTGCCA GGCTTTGAGT CTCCTGCAGG GCCCACCCCC
            841 TCACCCTCCG CACCGCTCGC TCCTCCGTGG CAAGGCCGCA GCATAGCTAG CTCCAAGCTC
            901 TGGATGTTGG AGTTCTCGGC TTTCCTGGAG CGCCAGCAAG ATCCGGGACA CATACAACAA
            961 ACACCTGTTT GTGCACATCA GCCCAGTCAA GCCCAAAGCT ACAGTGACCC CTACCTCGAA
           1021 CTGTGGACAT CCCGCCCAAT CTATGACAGT TCCCAGAAGA GAATGGAGCC TCACAAGCTA
          1081 TTTGACCGGG TCCTCTATGC TTTCTTCTGT GAAGGTCTGG CAGACCCCTC CA
          //
          Primer: IRES-R ()
          Sequence file: RDB12176_A6Ajb.seq check
          >12176_12176_A6Aj_IRES-R_D08_11.ab1
              1 GCACGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGGGCGGAAT
             61 TTACGTAGCG GCCGCGGCGC GCCGGCCCTC GAGGTAGAAG GCACAGTCGA GGCTGATCAG
            121 CGGGTTTAAA CGGGCCCTCT AGATCCTACC CACCGTACTC GTCAATTCCA AGGGCATCGG
            181 TAAACATCTG CTCAAACTCG AAGTCGGCCA TATCCAGAGC GCCGTAGGGG GCGGAGTCGT
            241 GGGGGGTAAA TCCCGGACCC GGGGAATCCC CGTCCCCCAA CATGTCCAGA TCGAAATCGT
            301 CTAGCGCGTC GGCATGCGCC ATCGCCACGT CCTCGCCGTC TAAGTGGAGC TCGTCCCCCA
            361 GGCTGACATC GGTCGGGGGG GCATCGATGT TGGTATTGAG GTCTGCCCAG AACTTCACAA
            421 GGAAGAAGGC ATTAGAGGGC CCCCGTTCAA ATAGCTCCTT GAGGCCTCCC TTCTTCTCTG
            481 GGAACTTGTC ATAGATTTGG CGGATGTCCA CAGTTTCGAG GTAGGGGTCA CTGTAGCTTG
            541 GGCTTGACTG GCTGATGTGC ACAAACAGGT GTTTGTTGTA TGTGTCCGGA TCTTGCTGGC
            601 GCTCCAGGAA AGCCGAGAAC TCCAACATCC AGAGCTTGGA GCTAGCTATG CTGCGGCCTT
            661 GCCACGGAGG AGCGAGCGGT GCGGAGGGTG AGGGGGTGGG CCCTGCAGGA GACTCAAAGC
            721 CTGGCAGAGG CAGCGGAGGC TGGACAGGGT AGGTGTTTTG AGAGAAAGGT TTCACATCGT
            781 GGGATGTTCC AGGTTGGCCT GGCAAAGCTC CTTGCCAAAA CCCTGAGATT GCTGGGTAGC
            841 CAGGGCCCCG GGCAAGAGCC ATTTTACTGT GGAAGGCTGT GGCCGAGACG ATCTGTGCGG
            901 ACGACATGGC AGCCATGCTC TGCAGGGCCT TGTTCTTAGC TGCCTGGTCC CTTGAGTTTG
            961 GCCTGGATCT CCCGGGCTTT TCGACGGGCA AGCACCTGGA TGTGGCTGGG AGACCTGCTT
           1021 CCTTTGTGCG CGTCTTTCCC TGTCCTGAGC TTTGATATGG GCGTGGCGAT CAGCTCATTC
           1081 GACCATAACA TCTTGCCGTC CTCGTCAAGA ATAATTTGCC GCGCGGCAGG GATGGGATGA
          1141 TGCCAGGACT CTGGAAGACC TTTTCG
          //
          Primer: IRES-F ()
          Sequence file: RDB12176_A6Ajc.seq check
          >12176_12176_A6Aj_IRES-F_F08_17.ab1
              1 TCCAATCCGT TTCTGTGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC GAACCACGGG
             61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG
            121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG
            181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT
            241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT
            301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT
            361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG
            421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA
            481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA
            541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA
            601 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA
            661 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA
            721 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
            781 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC
            841 AGCACAGTGG TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGG CCCGCGATTA
            901 GTCCAATTTG TTAAAGACAG GATATCAGTG GTCCAGGCTC TAGTTTTGAC TCAACAATAT
            961 CACCAGCTGA AGCCTATAGA GTACGAGCCA TAGATAGAAT AAAAGATTTT ATTTAGTCTC
           1021 AGAAAAAGGG GGGGAAATGA AGACCCCACT GTAGTTTGGG CAAGCTTAGC TAGTACGCCA
           1081 TTTTGCAAGC ATGGTAAAAT ACATAACCTG AGATAGAGAA GTTCAGGAAT TCAAGGCTTT
          1141 AG
          //
          Primer: Amp_R ()
          Sequence file: RDB12176_A6Ajd.seq check
          >12176_12176_A6Aj_Amp_R_G08_20.ab1
              1 TTCGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
             61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGCATAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG
            181 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAAAAGTTC AGATCAAGGT CAGGAACAGA
            241 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
            301 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
            361 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA
            421 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
            481 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
            541 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGACTGAGT
            601 CGCCCGGGTA CCCGTATTCC CAATAAAGCC TCTTGCTGTT TGCATCCGAA TCGTGGACTC
            661 GCTGATCCTT GGGAGGGTCT CCTCAGATTG ATTGACTGCC CACCTCGGGG GTCTTTCATT
            721 TGGAGGTTCC ACCGAGATTT GGAGACCCCT GCCCAGGGAC CACCGACCCC CCCGCCGGGA
            781 GGTAAGCTGG CCAGCGGTCG TTTCGTGTCT GTCTCTGTCT TTGTGCGTGT TTGTGCCGGC
            841 ATCTAATGTT TGCGCCTGCG TCTGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC
            901 CGTGGTGGAA CTGACGAGTT CGGAACACCC GGGCCGCAAC CCTGGAGACG TCCAGGACTT
            961 CGGGGGCCGT TTTGTGCCCG ACTGAGTCAA AATCCGATCG GTTTGACTCT TGTGCACCCC
           1021 CCTTATAGGA AATTGTGATC TGTTAGAGAC GAACCTAAAC AGTTCGCTTC GACTGATTAG
          1081 CATCGATGGA CGAGCGGCGG GCGACCTGAC T
          //
          Primer: pMFG-1 ()
          Sequence file: RDB12176_A6Aje.seq check
          >12176_12176_A6Aj_pMFG-1_H08_23.ab1
              1 AAAAGGGGGG GAAGGGCTGT TAGTAACTCT TGTCATGTCA GGGTCAGGGA AGTTTACAAG
             61 GGGCGGGGGT GCCCCATATA AGATCTCATA TGGCCATATG GGGGCGCCTA GAGAAGGAGT
            121 GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA GGGGGAGAGA
            181 CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT TGACCCAGGG
            241 AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT CTGGGTGTCC
            301 ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC GGTTAAAGGT
            361 GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA AGGTAACCCA
            421 ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT CTTTCCAGTG
            481 ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG ACAAATACAG
            541 AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CAGACAAGAC GCGCGGCGCG
            601 GCTTCGGTCC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT TTAGGTTCTC
            661 GTCTCCTACC AGAACCACAT ATCCCTCCTA TTAGGGGGGT GCACCAAAGA GTCCAAAACG
            721 ATCGGGATTT TTGGACTCAG GTCGGGCCAC AAAAACGGCC CCCGAAGTCC CTGGGACGTC
            781 TCCCAGGGTT GCGGCCGGGT GTTCCGAACT CGTCAGTTCC ACCACGGGTC CGCCAGATAC
            841 AGAGCTAGTT AGCTAACTAG TACAGACGCA GGCGCAAACA TTAGATGCCG GCACAAACAC
            901 GCACAAAGAC AGAGACAGAC ACGAAACGAC CGCTGGGCCA GCTTACCTCC CGGCGGGGGG
            961 GGTCGGTGGT CCCTGGGCAG GGGTCTCCAA ATCTCGGTGG AACCCTCCAA ATGAAAGACC
           1021 CCCGGAGTGG GCAGTCAATC AATCTGAAGG AGACCCCTCC CAAAGAATCA GCGAAGTCCA
           1081 CGATCGATGC AAACAGCCAA GAAGGCCTTT ATGGAAATAC GGGTACCGGG CGACTCAGTT
          1141 CAATTCGAGA CTGCCGCCGA ATTAGAGGTA
          //
          Primer: EGFP-C ()
          Sequence file: RDB12176_A6Ajf.seq check
          >12176_12176_A6Aj_EGFP-C_A09_03.ab1
              1 CAGGGGAATC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG
             61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT
            121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA
            181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG
            241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA
            301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA
            361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC
            421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT
            481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC
            541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT
            601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC
            661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC
            721 GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT
            781 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGGTCT TTCACATGCA
            841 GCATGTATCA AAATTAATTT GGTTTTTTTT CTTAAGTATT TACATTAAAT GGCCATAGTT
            901 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
            961 TTCCTCGCTC ACTGACTCGC TGCGCTCGGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
           1021 ACTCAAAGGC GTAATACGGT TATCCACAGA AATCAGGGGA TACGCAGAAA GAACATGTGG
          1081 AGCCAAGGCA GCAACGCCAA GGAACCGTAA AAGGCCCGCG ATTTACCTTT G
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl