Resource data sheet
Prev.

pMYs-Tead4-VP16-IRES-EGFP

Retroviral vector of mouse Tead4, constitutive active

Catalog number RDB12176
Resource name pMYs-Tead4-VP16-IRES-EGFP
Clone info. Retroviral vector of mouse Tead4, activation domain of herpes simplex virus VP16, constitutive active.
Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: C to G substitution (Pro, synonym) at 1265 nt of NM_011567.2.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead4 cDNA HSV VP16 protein cDNA
Depositor Sasaki, Hiroshi |
 
Other clones in our bank mouse Tead4 (NCBI Gene 21679) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions for distribution Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Schedule A (GFP-A) [Link]
Remarks Remember that you will be working with samples containing infections virus.
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12176 pMYs-Tead4-VP16-IRES-EGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12176_A6Ajp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') RDB12176_A6Aja.seq
>12176_12176_A6Aj_5'Retro(pFB-5')_E08_14.ab1
    1 TTCAGCTAAG ATTCATCTCG GACTGCTAGT TATTAAGGAT CCCAGTGTGG TGGTACGGGA
   61 ATTCCTGCAG GGTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT
  121 AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT
  181 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGTACCAGG ATCCAGTGTG
  241 GTGGAATTCA TTACCTCCAA CGAGTGGAGC TCTCCCGACT CCCCCGAGGG GAGCAGCATC
  301 TCTGGGGGCA GCCAGGCACT GGACAAGCCC ATCGACAATG ATGCAGAGGG TGTATGGAGC
  361 CCCGAAATTG AGCGAAGCTT CCAGGAGGCC CTGGCCATCT ACCCACCCTG CGGCCGCCGC
  421 AAAATTATCC TGACGGAGGA AGGCAAGATG TATGGTCGGA ATGAGCTGAT CGCACGCCAT
  481 ATCAAGCTCA GGACAGGGAA GACGCGCACA AGGAAGCAGG TCTCCAGCCA CATCCAGGTG
  541 CTTGCCCGTC GAAAAGCCCG GGAGATCCAG GCCAAACTCA AGGACCAGGC AGCTAAGAAC
  601 AAGGCCCTGC AGAGCATGGC TGCCATGTCG TCCGCACAGA TCGTCTCGGC CACAGCCTTC
  661 CACAGTAAAA TGGCTCTTGC CCGGGGCCCT GGCTACCCAG CAATCTCAGG GTTTTGGCAA
  721 GGAGCTTTGC CAGGCCAACC TGGAACATCC CACGATGTGA AACCTTTCTC TCAAAACACC
  781 TACCCTGTCC AGCCTCCGCT GCCTCTGCCA GGCTTTGAGT CTCCTGCAGG GCCCACCCCC
  841 TCACCCTCCG CACCGCTCGC TCCTCCGTGG CAAGGCCGCA GCATAGCTAG CTCCAAGCTC
  901 TGGATGTTGG AGTTCTCGGC TTTCCTGGAG CGCCAGCAAG ATCCGGGACA CATACAACAA
  961 ACACCTGTTT GTGCACATCA GCCCAGTCAA GCCCAAAGCT ACAGTGACCC CTACCTCGAA
 1021 CTGTGGACAT CCCGCCCAAT CTATGACAGT TCCCAGAAGA GAATGGAGCC TCACAAGCTA
1081 TTTGACCGGG TCCTCTATGC TTTCTTCTGT GAAGGTCTGG CAGACCCCTC CA
//
Primer: IRES-R RDB12176_A6Ajb.seq
>12176_12176_A6Aj_IRES-R_D08_11.ab1
    1 GCACGCGGCT TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGGGGGG GGGGCGGAAT
   61 TTACGTAGCG GCCGCGGCGC GCCGGCCCTC GAGGTAGAAG GCACAGTCGA GGCTGATCAG
  121 CGGGTTTAAA CGGGCCCTCT AGATCCTACC CACCGTACTC GTCAATTCCA AGGGCATCGG
  181 TAAACATCTG CTCAAACTCG AAGTCGGCCA TATCCAGAGC GCCGTAGGGG GCGGAGTCGT
  241 GGGGGGTAAA TCCCGGACCC GGGGAATCCC CGTCCCCCAA CATGTCCAGA TCGAAATCGT
  301 CTAGCGCGTC GGCATGCGCC ATCGCCACGT CCTCGCCGTC TAAGTGGAGC TCGTCCCCCA
  361 GGCTGACATC GGTCGGGGGG GCATCGATGT TGGTATTGAG GTCTGCCCAG AACTTCACAA
  421 GGAAGAAGGC ATTAGAGGGC CCCCGTTCAA ATAGCTCCTT GAGGCCTCCC TTCTTCTCTG
  481 GGAACTTGTC ATAGATTTGG CGGATGTCCA CAGTTTCGAG GTAGGGGTCA CTGTAGCTTG
  541 GGCTTGACTG GCTGATGTGC ACAAACAGGT GTTTGTTGTA TGTGTCCGGA TCTTGCTGGC
  601 GCTCCAGGAA AGCCGAGAAC TCCAACATCC AGAGCTTGGA GCTAGCTATG CTGCGGCCTT
  661 GCCACGGAGG AGCGAGCGGT GCGGAGGGTG AGGGGGTGGG CCCTGCAGGA GACTCAAAGC
  721 CTGGCAGAGG CAGCGGAGGC TGGACAGGGT AGGTGTTTTG AGAGAAAGGT TTCACATCGT
  781 GGGATGTTCC AGGTTGGCCT GGCAAAGCTC CTTGCCAAAA CCCTGAGATT GCTGGGTAGC
  841 CAGGGCCCCG GGCAAGAGCC ATTTTACTGT GGAAGGCTGT GGCCGAGACG ATCTGTGCGG
  901 ACGACATGGC AGCCATGCTC TGCAGGGCCT TGTTCTTAGC TGCCTGGTCC CTTGAGTTTG
  961 GCCTGGATCT CCCGGGCTTT TCGACGGGCA AGCACCTGGA TGTGGCTGGG AGACCTGCTT
 1021 CCTTTGTGCG CGTCTTTCCC TGTCCTGAGC TTTGATATGG GCGTGGCGAT CAGCTCATTC
 1081 GACCATAACA TCTTGCCGTC CTCGTCAAGA ATAATTTGCC GCGCGGCAGG GATGGGATGA
1141 TGCCAGGACT CTGGAAGACC TTTTCG
//
Primer: IRES-F RDB12176_A6Ajc.seq
>12176_12176_A6Aj_IRES-F_F08_17.ab1
    1 TCCAATCCGT TTCTGTGTTT AGTCGAGGTT AAAAAACGTC TAGGCCCCCC GAACCACGGG
   61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG
  121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG
  181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT
  241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT
  301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT
  361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG
  421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA
  481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA
  541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA
  601 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA
  661 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA
  721 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT
  781 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC
  841 AGCACAGTGG TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGG CCCGCGATTA
  901 GTCCAATTTG TTAAAGACAG GATATCAGTG GTCCAGGCTC TAGTTTTGAC TCAACAATAT
  961 CACCAGCTGA AGCCTATAGA GTACGAGCCA TAGATAGAAT AAAAGATTTT ATTTAGTCTC
 1021 AGAAAAAGGG GGGGAAATGA AGACCCCACT GTAGTTTGGG CAAGCTTAGC TAGTACGCCA
 1081 TTTTGCAAGC ATGGTAAAAT ACATAACCTG AGATAGAGAA GTTCAGGAAT TCAAGGCTTT
1141 AG
//
Primer: Amp_R RDB12176_A6Ajd.seq
>12176_12176_A6Aj_Amp_R_G08_20.ab1
    1 TTCGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGCATAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA GCTTAAGTAA CGCCATTTTG
  181 CAAGGCATGG AAAAATACAT AACTGAGAAT AGAAAAGTTC AGATCAAGGT CAGGAACAGA
  241 TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC
  301 AGGGCCAAGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG
  361 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA
  421 GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC
  481 CTTATTTGAA CTAACCAATC AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG
  541 AGCTCAATAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TTGACTGAGT
  601 CGCCCGGGTA CCCGTATTCC CAATAAAGCC TCTTGCTGTT TGCATCCGAA TCGTGGACTC
  661 GCTGATCCTT GGGAGGGTCT CCTCAGATTG ATTGACTGCC CACCTCGGGG GTCTTTCATT
  721 TGGAGGTTCC ACCGAGATTT GGAGACCCCT GCCCAGGGAC CACCGACCCC CCCGCCGGGA
  781 GGTAAGCTGG CCAGCGGTCG TTTCGTGTCT GTCTCTGTCT TTGTGCGTGT TTGTGCCGGC
  841 ATCTAATGTT TGCGCCTGCG TCTGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC
  901 CGTGGTGGAA CTGACGAGTT CGGAACACCC GGGCCGCAAC CCTGGAGACG TCCAGGACTT
  961 CGGGGGCCGT TTTGTGCCCG ACTGAGTCAA AATCCGATCG GTTTGACTCT TGTGCACCCC
 1021 CCTTATAGGA AATTGTGATC TGTTAGAGAC GAACCTAAAC AGTTCGCTTC GACTGATTAG
1081 CATCGATGGA CGAGCGGCGG GCGACCTGAC T
//
Primer: pMFG_1 RDB12176_A6Aje.seq
>12176_12176_A6Aj_pMFG-1_H08_23.ab1
    1 AAAAGGGGGG GAAGGGCTGT TAGTAACTCT TGTCATGTCA GGGTCAGGGA AGTTTACAAG
   61 GGGCGGGGGT GCCCCATATA AGATCTCATA TGGCCATATG GGGGCGCCTA GAGAAGGAGT
  121 GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA GGGGGAGAGA
  181 CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT TGACCCAGGG
  241 AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT CTGGGTGTCC
  301 ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC GGTTAAAGGT
  361 GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA AGGTAACCCA
  421 ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT CTTTCCAGTG
  481 ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG ACAAATACAG
  541 AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CAGACAAGAC GCGCGGCGCG
  601 GCTTCGGTCC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT TTAGGTTCTC
  661 GTCTCCTACC AGAACCACAT ATCCCTCCTA TTAGGGGGGT GCACCAAAGA GTCCAAAACG
  721 ATCGGGATTT TTGGACTCAG GTCGGGCCAC AAAAACGGCC CCCGAAGTCC CTGGGACGTC
  781 TCCCAGGGTT GCGGCCGGGT GTTCCGAACT CGTCAGTTCC ACCACGGGTC CGCCAGATAC
  841 AGAGCTAGTT AGCTAACTAG TACAGACGCA GGCGCAAACA TTAGATGCCG GCACAAACAC
  901 GCACAAAGAC AGAGACAGAC ACGAAACGAC CGCTGGGCCA GCTTACCTCC CGGCGGGGGG
  961 GGTCGGTGGT CCCTGGGCAG GGGTCTCCAA ATCTCGGTGG AACCCTCCAA ATGAAAGACC
 1021 CCCGGAGTGG GCAGTCAATC AATCTGAAGG AGACCCCTCC CAAAGAATCA GCGAAGTCCA
 1081 CGATCGATGC AAACAGCCAA GAAGGCCTTT ATGGAAATAC GGGTACCGGG CGACTCAGTT
1141 CAATTCGAGA CTGCCGCCGA ATTAGAGGTA
//
Primer: EGFP-C RDB12176_A6Ajf.seq
>12176_12176_A6Aj_EGFP-C_A09_03.ab1
    1 CAGGGGAATC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG
   61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT
  121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA
  181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG
  241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA
  301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA
  361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC
  421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT
  481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC
  541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT
  601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC
  661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC
  721 GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT
  781 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGGTCT TTCACATGCA
  841 GCATGTATCA AAATTAATTT GGTTTTTTTT CTTAAGTATT TACATTAAAT GGCCATAGTT
  901 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
  961 TTCCTCGCTC ACTGACTCGC TGCGCTCGGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
 1021 ACTCAAAGGC GTAATACGGT TATCCACAGA AATCAGGGGA TACGCAGAAA GAACATGTGG
1081 AGCCAAGGCA GCAACGCCAA GGAACCGTAA AAGGCCCGCG ATTTACCTTT G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

2019.08.06

GNP_filter3_RDBDEP_html_190432.pl