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Nozomu Nishi's Resource

Nozomu Nishi's web page in English /  in Japanese.

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Catalog #Name of cloneRunning titlePublication
RDB04217pGEX-G1-1aBacteria expression vector of human galectin-1, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB04218pGEX-G2-1aBacteria expression vector of human galectin-2, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB04219pGEX-G3-1aBacteria expression vector of human galectin-3, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB04220pTrc-G4-1aBacteria expression vector of human galectin-4, (His) 6 -containing-tag proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB04221pGEX-G7-5aBacteria expression vector of human galectin-7, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB04222pGEX-G8(M)-2aBacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB04223pGEX-G9(M)-1aBacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M), GST fusion proteinMatsushita, N., Requirement of divalent galactoside-binding activity of ecalectin/galectin-9 for eosinophil chemoattraction. J. Biol. Chem., 275, 8355-8360 (2000). PMID 10722666. (original).
RDB04224pTrc-G10-4aBacteria expression vector of human galectin-10, (His) 6 -containing-tag proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB04225pGEX-LNG12-3Bacteria expression vector of human galectin-12 with an N-terminally extended NCRD (LNG12), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08392pET-G1-1aBacteria expression vector of human galectin-1, tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08393pET-G3-1aBacteria expression vector of human galectin-3, tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08394pGEX-G3CRD-1aBacteria expression vector of human galectin-3 (C-terminal lectin domain/carbohydrate recognition domain, G3CRD), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08395pET-G3CRD-1aBacteria expression vector of human galectin-3 (C-terminal lectin domain/carbohydrate recognition domain, G3CRD), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08396pGEX-G4-1aBacteria expression vector of human galectin-4, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08398pGEX-G4CT-1aBacteria expression vector of human galectin-4 (C-terminal CRD with a part of the linker peptide, G4CT), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08399pET-G7-1aBacteria expression vector of human galectin-7, tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08400pGEX-G7 R54H-12aBacteria expression vector of human galectin-7 with an R54H mutation, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08401pGEX-G8(M) R69H-1aBacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M) with an R69H mutation, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08402pGEX-G8(M) R233H-2aBacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M) with an R233H mutation, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08403pGEX-G8(M) R69, 233H-1aBacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M) with R69,233H mutations, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08404pET-G8(M)-1aBacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08405pGEX-G8(L)-1aBacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08406pGEX-G8(L) R69H-1aBacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L) with an R69H mutation, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08407pGEX-G8(L) R275H-3aBacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L) with an R275H mutation, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08408pGEX-G8(L) R69, 275H-1aBacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L) with R69,275H mutations, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08409pGEX-G8(L) R197A-1aBacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L) with an R197A mutation, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08410pET-G8(L)-12aBacteria expression vector of human galectin-8 with a long linker peptide (G8(L)/G8L), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08411pGEX-G8NT-1aBacteria expression vector of human galectin-8 (N-terminal CRD with a part of the linker peptide, G8NT), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08412pGEX-G8CT-1aBacteria expression vector of human galectin-8 (C-terminal CRD with a part of the linker peptide, G8CT), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08413pGEX-G8NCRD-1aBacteria expression vector of human galectin-8 (N-terminal CRD, G8NCRD), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08414pGEX-G8CCRD-1aBacteria expression vector of human galectin-8 (C-terminal CRD, G8CCRD), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08415pGEX-G9(S)-1aBacteria expression vector of human galectin-9 with a short linker peptide (G9(S)/G9S), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08416pET-G9(S)-3aBacteria expression vector of human galectin-9 with a short linker peptide (G9(S)/G9S), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08417pGEX-G9(M) R65H-2aBacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M) with an R65H mutation, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08418pGEX-G9(M) R239H-1aBacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M) with an R239H mutation, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08419pGEX-G9(M) R65, 239H-1aBacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M) with R65,239H mutations, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08420pET-G9(M)-4aBacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08421pGEX-G9(L)-2aBacteria expression vector of human galectin-9 with a long linker peptide (G9(L)/G9L), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08422pET-G9(L)-5aBacteria expression vector of human galectin-9 with a long linker peptide (G9(L)/G9L), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08423pGEX-G9NT-1aBacteria expression vector of human galectin-9 (N-terminal CRD with a part of the linker peptide, G9NT), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08424pGEX-G9CT-1aBacteria expression vector of human galectin-9 (C-terminal CRD with a part of the linker peptide, G9CT), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08425pGEX-G9NCRD-1aBacteria expression vector of human galectin-9 (N-terminal CRD, G9NCRD), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08426pGEX-G9CCRD-1aBacteria expression vector of human galectin-9 (C-terminal CRD, G9CCRD), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08427pGEX-G10-8aBacteria expression vector of human galectin-10, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08428pGEX-G12-1aBacteria expression vector of human galectin-12, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08429pGEX-G12NT-1bBacteria expression vector of human galectin-12 (N-terminal CRD with a part of the linker peptide, G12NT), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08430pGEX-G12CT-1bBacteria expression vector of human galectin-12 (C-terminal CRD with a part of the linker peptide, G12CT), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08431pGEX-G13-1aBacteria expression vector of human galectin-13, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08432pGEX-PPL13-1aBacteria expression vector of human galectin-14/PPL13, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08433pGEX-mG1-1aBacteria expression vector of mouse galectin-1, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08434pET-mG1-1aBacteria expression vector of mouse galectin-1, tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08435pGEX-mG2-1aBacteria expression vector of mouse galectin-2, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08436pGEX-mG3-1aBacteria expression vector of mouse galectin-3, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08437pGEX-mG4-1aBacteria expression vector of mouse galectin-4, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08438pGEX-mG7-7aBacteria expression vector of mouse galectin-7, GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08439pET-mG7-1aBacteria expression vector of mouse galectin-7, tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08440pGEX-mG8(M)-2aBacteria expression vector of mouse galectin-8 (the predominant isoform of mG8 in most cell and tissue types, mG8(M)/mG8M), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08441pET-mG8(M)-1aBacteria expression vector of mouse galectin-8 (the predominant isoform of mG8 in most cell and tissue types, mG8(M)/mG8M), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08442pGEX-mG9(M)-2aBacteria expression vector of mouse galectin-9 (the predominant isoform of mG9 in most cell and tissue types (mG9(M)/mG9M), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08443pET-mG9(M)-1aBacteria expression vector of mouse galectin-9 (the predominant isoform of mG9 in most cell and tissue types (mG9(M)/mG9M), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08444pET-mG9(L)-3aBacteria expression vector of mouse galectin-9 with a long linker peptide (mG9(L)/mG9L), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08445pGEX-RaG8(M)-1aBacteria expression vector of rat galectin-8 (the predominant isoform of rat G8 in most cell and tissue types, RaG8(M)/RaG8M), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08446pET-RaG8(M)-1aBacteria expression vector of rat galectin-8 (the predominant isoform of rat G8 in most cell and tissue types, RaG8(M)/RaG8M), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08447pTrc-RaG9(M)-2aBacteria expression vector of rat galectin-9 (the predominant isoform of RaG9 in most cell and tissue types (RaG9(M)/RaG9M), (His) 6 -containing-tag proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08448pGEX-CSG1-2aBacteria expression vector of human galectin-1 in which all Cys residues are substituted with Ser residues (G1C3,17,43,61,89,131S: CSG1), GST fusion proteinNishi, N., Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1. Glycobiology 18: 1065-1073 (2008). PMID 18796645. (original).
RDB08449pET-CSG1-1aBacteria expression vector of human galectin-1 in which all Cys residues are substituted with Ser residues (G1C3,17,43,61,89,131S: CSG1), tag-free proteinNishi, N., Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1. Glycobiology 18: 1065-1073 (2008). PMID 18796645. (original).
RDB08451pET-G9Null-11aBacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null), tag-free proteinNishi, N., Development of highly stable galectins: truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins. FEBS Lett. 579: 2058-2064 (2005). PMID 15811318. (original).
RDB08452pET-G9Null R65D-2aBacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null) with an R65D mutation, tag-free proteinNishi, N., Development of highly stable galectins: truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins. FEBS Lett. 579: 2058-2064 (2005). PMID 15811318. (original).
RDB08453pET-G9Null R212D-3aBacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null) with an R212D mutation, tag-free proteinNishi, N., Development of highly stable galectins: truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins. FEBS Lett. 579: 2058-2064 (2005). PMID 15811318. (original).
RDB08454pGEX-G8Null-1aBacteria expression vector of human galectin-8 with a truncated linker peptide (G8Null), GST fusion proteinNishi, N., Development of highly stable galectins: truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins. FEBS Lett. 579: 2058-2064 (2005). PMID 15811318. (original).
RDB08455pET-G8Null-5aBacteria expression vector of human galectin-8 with a truncated linker peptide (G8Null), tag-free proteinNishi, N., Development of highly stable galectins: truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins. FEBS Lett. 579: 2058-2064 (2005). PMID 15811318. (original).
RDB08456pET-mG8Null-2aBacteria expression vector of mouse galectin-8 with a truncated linker peptide (mG8Null), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08457pET-mG9Null-5aBacteria expression vector of mouse galectin-9 with a truncated linker peptide (mG9Null), tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08458pET-mG9Null R64H-1aBacteria expression vector of mouse galectin-9 with a truncated linker peptide (mG9Null) with an R64H mutation, tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB08459pET-mG9Null R211H-2aBacteria expression vector of mouse galectin-9 with a truncated linker peptide (mG9Null) with an R211H mutation, tag-free proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB15282pET-ssG9Bacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null) with a 10-amino acid deletion and a single amino acid mutation at the remaining linker peptide (ssG9), tag-free proteinItoh, A., Optimization of the inter-domain structure of galectin-9 for recombinant production. Glycobiology 23 (8): 920-925 (2013). PMID 23507964. (original).
RDB15983mIGEHMouse cDNA for IgE H chain.Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205. (original).
RDB15984mIGELMouse cDNA for IgE L chain kappa.Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205. (original).
RDB15985pELN-1Bacterial expression vector of porcine elastin (ELN) long form.Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189. (original).
RDB15986pELN-2Bacterial expression vector of porcine elastin (ELN) short form.Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189. (original).
RDB15987pOGNPorcine cDNA for osteoglycin/mimecan (OGN).Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189. (original).
RDB18237pGEX-G4NTBacteria expression vector of human galectin-4 (N-terminal CRD with a part of the linker peptide, G4NT), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB18238pGEX-G4NullBacteria expression vector of human galectin-4 with a truncated linker peptide (G4Null), GST fusion proteinNishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB19436pET-mG3-2Bacterial expression vector of mouse galectin-3 (tag-free protein).Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB19437pET-G4-1Bacterial expression vector of human galectin-4 (tag-free protein).Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).
RDB19438pET-G4NullBacterial expression vector of human galectin-4 with a partially truncated linker peptide (tag-free protein).Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). (protocol).

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2022.07.27

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