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pGEX-mG9(M)-2a

Bacteria expression vector of mouse galectin-9 (the predominant isoform of mG9 in most cell and tissue types (mG9(M)/mG9M), GST fusion protein

Catalog number RDB08442
Resource name pGEX-mG9(M)-2a
Clone info. Bacterial expression clone of mouse galectin-9.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse galectin-9 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Mouse galectin-9

          Reference sequence
            

          Distribution information

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          RDB08442 pGEX-mG9(M)-2a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08442z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08442_A2Crp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST, thrombin site, insert 5'
          Sequence file: RDB08442_A2Cra.seq check
          >08442_08442_B1HbF_pGEX-F2_D04_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTGN NGCTATCCNN CNAATTGATA AGTACTTGAA
             61 ATCCAGCAAG TATATAGCAT GGCCTTTGCA GGGCTGGCAA GCCACGTTTG GTGGTGGCGA
            121 CCATCCTCCA AAATCGGATC TGGTTCCGCG TGGATCCATG GCTCTCTTCA GTGCCCAGTC
            181 TCCATACATT AACCCGATCA TCCCCTTTAC TGGACCAATC CAAGGAGGGC TGCAGGAGGG
            241 ACTTCAGGTG ACCCTCCAGG GGACTACCAA GAGTTTTGCA CAAAGGTTTG TGGTGAACTT
            301 TCAGAACAGC TTCAATGGAA ATGACATTGC CTTCCACTTC AACCCCCGGT TTGAGGAAGG
            361 AGGGTATGTG GTTTGCAACA CGAAGCAGAA CGGACAGTGG GGTCCTGAGG AGAGAAAGAT
            421 GCAGATGCCC TTCCAGAAGG GGATGCCCTT TGAGCTTTGC TTCCTGGTGC AGAGGTCAGA
            481 GTTCAAGGTG ATGGTGAACA AGAAATTCTT TGTGCAGTAC CAACACCGCG TACCCTACCA
            541 CCTCGTGGAC ACCATCGCTG TCTCCGGCTG CTTGAAGCTG TCCTTTATCA CCTTCCAGAC
            601 TCAGAACTTT CGTCCTGCCC ACCAGGCACC CATGGCTCAA ACTACCATCC ATATGGTTCA
            661 CAGCACCCCT GGACAGATGT TCTCTACTCC TGGAATCCCT CCTGTGGTGT ACCCCACCCC
            721 AGCCTATACC ATACCTTTCT ACACCCCCAT TCCAAATGGG CTTTACCCGT CCNAGTCCAT
            781 CATGATATCA GGCAATGTCT TGCCAGATGC TACGAGGTTC CATATCAACC TTCGCTGTGG
            841 ANGTGANATT NCTTTCCACC NGANCCCCCC GTTTCAATGA NAANGCTGTT GTCCGAAACA
            901 NTCANNTNNN CAACTCCNGG GGGGCAGGNA NANCGNANNT CTGCTTNGGA NGNNGCCCNT
          961 NNANNCNANG NCCNNANCTT NNCNGNNNNN NNNNN
          //
          Primer: pJB8-Forward
          Region: insert 3'
          Sequence file: RDB08442_A2Crb.seq check
          >08442_08442_B1HbF_pJB8-Forward_E04_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN TNGGTNNNGG CTGCGCCCCG NCNCCCGCCA ACACCCGCTG
             61 ACGCGCCCTG ACGGGCTTGT CNNCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT
            121 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGATCG
            181 TCAGTCAGTC ACGATGCGGC CGCTCGAGTC GACCCGGGAA TTCCTATGTC TGCACGTGGG
            241 TCAGCTGGAT ATCACCCGCC ACTTCTAGAG TGTTGATATC CTGCAAGTTC TTCAGGCGGT
            301 GGTAATATTC ACACATGTGT TGACCATTCA CAGCTACCTT GAAGCAGTGA CCTTCACATA
            361 TGATCCACAC CGAGAAGCTC TGGCCTCGAC TGAAGGGCAT CCTCCCAAGC AGACTTCGCT
            421 CTTCCTGCCC CCAGGAGTTG TTGATCTGAG TGTTTCGGAC AACAGCATTC TCATTGAAAC
            481 GGGGGTTCAG GTGGAAAGCA ATGTCACCTC CACAGCGAAG GTTGATATGG AACCTCGTAG
            541 CATCTGGCAA GACATTGCCT GATATCATGA TGGACTTGGA CGGGTAAAGC CCATTTGGAA
            601 TGGGGGTGTA GAAAGGTATG GTATAGGCTG GGGTGGGGTA CACCACAGGA GGGATTCCAG
            661 GAGTAGAGAA CATCTGTCCA GGGGTGCTGT GAACCATATG GATGGTAGTT TGAGCCATGG
            721 GTGCCTGGTG GGCAGGACGA AAGTTCTGAN TCTGGAANGT GATAAAGGAC AGCTTCAAGC
            781 AGCCGGAGAC AGCGATGGTG TCCACGANGN GGTANGGTAC GCGGTGTTGG TACTGCACAA
            841 AGAANTTCTT GTTCACCATC ACCTTGAACT CTGANCTCTG CACCNNNAAN CAAAGCTCAA
          901 AGGGCATNCC CTTCTGNNAN NNNNTCTGCA TCTTTCTNNN NNNNNNN
          //
          Primer: lacIpro-B_F
          Region: lacI pro, lacI
          Sequence file: RDB08442_A2Crc.seq check
          >08442_08442_B1HbF_lacIpro-B_F_F04_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNANCGNNN CNGTGANCGN GGNNGCGGAA GANCGCCTGN
             61 TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATAAAT TCCGACACCA
            121 TCGAATGGCG CAAAACCTTT CGCGGTATGG CATGATAGCG CCCGGAAGAG AGTCAATTCA
            181 GGGTGGTGAA TGTGAAACCA GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT
            241 ATCAGACCGT TTCCCGCGTG GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA
            301 AAGTGGAAGC GGCGATGGCG GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG
            361 CGGGCAAACA GTCGTTGCTG ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT
            421 CGCAAATTGT CGCGGCGATT AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT
            481 CGATGGTAGA ACGAAGCGGC GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC
            541 AACGCGTCAG TGGGCTGATC ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG
            601 AAGCTGCCTG CACTAATGTT CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA
            661 ACAGTATTAT TTTCTCCCAT GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT
            721 TGGGTCACCA GCAAATCGCG CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC
            781 GNCTGGCTGG NTGGCATAAA TATCTCACTC GCAATCAAAT TCAGCCGATA NCGGAACGGG
            841 AANGCGACTG GANTGCCATG NCCNGGTTTT CNNNAAACNN TGCNAATGCT GANNGNNGNC
            901 ATCGTTCCCN CNNCNATGCT NGNTGNCNAC GATCNNATNG NGCTGGGNNN NANGNNNGNC
          961 NNTTNNNNAN NNCNNGNCTN NNCNNTNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl