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pGEX-mG1-1a

Bacteria expression vector of mouse galectin-1, GST fusion protein

Catalog number RDB08433
Resource name pGEX-mG1-1a
Clone info. Bacterial expression clone of mouse galectin-1.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse galectin-1 cDNA
Depositor|Developer Nishi, Nozomu |
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External Database
Mouse galectin-1

          Reference sequence
            

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
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          RDB08433 pGEX-mG1-1a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08433z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08433_A2Cmp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST, thrombin site, insert
          Sequence file: RDB08433_A2Cma.seq check
          >08433_08433_B1HbF_pGEX-F2_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT ANTGNNGCTA TCCCNCNAAT TGNTAAGTAC TTGAAATCCN
             61 GCAAGTATAT AGCATGGCCT TTGCAGGGCT GGCAAGCCAC GTTTGGTGGT GGCGACCATC
            121 CTCCAAAATC GGATCTGGTT CCGCGTGGAT CCATGGCCTG TGGTCTGGTC GCCAGCAACC
            181 TGAATCTCAA ACCTGGGGAA TGTCTCAAAG TTCGGGGAGA GGTGGCCTCG GACGCCAAGA
            241 GCTTTGTGCT GAACCTGGGA AAAGACAGCA ACAACCTGTG CCTACACTTC AATCCTCGCT
            301 TCAATGCCCA TGGAGACGCC AACACCATTG TGTGTAACAC CAAGGAAGAT GGGACCTGGG
            361 GAACCGAACA CCGGGAACCT GCCTTCCCCT TCCAGCCCGG GAGCATCACA GAGGTGTGCA
            421 TCACCTTTGA CCAGGCTGAC CTGACCATCA AGCTGCCAGA CGGACATGAA TTCAAGTTCC
            481 CCAACCGCCT CAACATGGAG GCCATCAACT ACATGGCGGC GGATGGAGAC TTCAAGATTA
            541 AGTGCGTGGC CTTTGAGTGA CTCGAGCGGC CGCATCGTGA CTGACTGACG ATCTGCCTCG
            601 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG
            661 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG
            721 GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTANCGA TAGCGGANTG TATAATTCTT
            781 GAAGACGAAA GGGCCTCGNG ATACGCCTAT TTTTATANGT TAATGTCATG ATAATAATGG
            841 TTTCTTANAC GTCANGNGNN ANTTTTCGGG GAAATGTGCN CGGAACCCCT ATTNGNTTAT
            901 TTTTCNNAAN ACANNNAAAT ATGTANNCNN NNCATGNNNN NNNNANCNNN NNNANNNNNN
          961 NNNN
          //
          Primer: lacIpro-B_F
          Region: lacI pro, lacI
          Sequence file: RDB08433_A2Cmb.seq check
          >08433_08433_B1HbF_lacIpro-B_F_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNNC GNNTCNGTGN NCGAGGAANC GGAAGANCGC CTGATGCGGT
             61 ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT AAATTCCGAC ACCATCGAAT
            121 GGCGCAAAAC CTTTCGCGGT ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG
            181 TGAATGTGAA ACCAGTAACG TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA
            241 CCGTTTCCCG CGTGGTGAAC CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG
            301 AAGCGGCGAT GGCGGAGCTG AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA
            361 AACAGTCGTT GCTGATTGGC GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA
            421 TTGTCGCGGC GATTAAATCT CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG
            481 TAGAACGAAG CGGCGTCGAA GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG
            541 TCAGTGGGCT GATCATTAAC TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG
            601 CCTGCACTAA TGTTCCGGCG TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA
            661 TTATTTTCTC CCATGAAGAC GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC
            721 ACCAGCAAAT CGCGCTGTTA GCGGGCCCAT TAAGTTCTGT CTCGGCGCGT CTGCGTCTGG
            781 CTGGCTGGCA TAANTATCTC ACTCGCAATC AAATTCAGCC GATAGCGGAA CGGGAANGCG
            841 ANNGGNGTGC CATGTCCGGT TTTCAACAAA CCATGCAAAT GCTGAATGAN GGCATCGTTC
            901 CCACNGCNNN GCTGGTTGCC NACNATCANA TGGNGCNNGN NGCNNNGNGC GCNNTTACCN
          961 ANNNNNGNNN GCNNNNNNGN NNNGNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl