Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-G4Null

Bacteria expression vector of human galectin-4 with a truncated linker peptide (G4Null), GST fusion protein

Catalog number RDB18238
Resource name pGEX-G4Null
Clone info. Bacterial expression clone of human galectin-4 with a truncated linker peptide.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human galectin-4 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Human galectin-4

          Reference sequence
            
           
          Sequence (partial) RDB18238pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースの利用は、学術機関の学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB18238 pGEX-G4Null DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18238_B0Gfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST, thrombin site, Insert 5'
          Sequence file: RDB18238_B0Gfa.seq check
          >D07204A3_B0Gf_2_pGEX-F2_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNTTNNANN NNGTATTGNN GCTATCCCNC AAATTGATAA GTACTTGAAA
             61 TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC
            121 CATCCTCCAA AATCGGATCT GGTTCCGCGT GGATCCCCAG GAATTCCCAT GGCCTATGTC
            181 CCCGCACCGG GCTACCAGCC CACCTACAAC CCGACGCTGC CTTACTACCA GCCCATCCCG
            241 GGCGGGCTCA ACGTGGGAAT GTCTGTTTAC ATCCAAGGAG TGGCCAGCGA GCACATGAAG
            301 CGGTTCTTCG TGAACTTTGT GGTTGGGCAG GATCCGGGCT CAGACGTCGC CTTCCACTTC
            361 AATCCGCGGT TTGACGGCTG GGACAAGGTG GTCTTCAACA CGTTGCAGGG CGGGAAGTGG
            421 GGCAGCGAGG AGAGGAAGAG GAGCATGCCC TTCAAAAAGG GTGCCGCCTT TGAGCTGGTC
            481 TTCATAGTCC TGGCTGAGCA CTACAAGGTG GTGGTAAATG GAAATCCCTT CTATGAGTAC
            541 GGGCACCGGC TTCCCCTACA GATGGTCACC CACCTGCAAG TGGATGGGGA TCTGCAACTT
            601 CAATCAATCA ACTTCATCGG AGGCCAGCCC CTCACCTTCA ACCCGCCTGT GCCATATTTC
            661 GGGAGGCTGC AAGGAGGGCT CACAGCTCGA AGAACCATCA TCATCAAGGG CTATGTGCCT
            721 CCCACAGGCA AGAGCTTTGC TATCAACTTC NAGGTGGGCT CCTCANGGGA NATAGCTCTG
            781 CACATTAATC CCCGCATGGG CAACGGTACC GNGGTCCGGA ACAGCCTTCT GAATGGCTCG
            841 TGGGGATNCG AGGANAANAA GATCACCCAC AACCCNTTTG GNNCCGGANA GTTCTTTGAT
            901 CTGNNNNTTC NCTGNGGNTN GNATNGCTTC NAGGTTTACG CNNATGNNCN GCACCNCTTT
          961 GANTTTGCCN NNNNNNNNTC NGNNNNNNNN NANGNNNNNN NNNN
          //
          Primer: pJB8-Forward
          Region: Insert 3'
          Sequence file: RDB18238_B0Gfb.seq check
          >D07204A3_B0Gf_2_pJB8-Forward_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGNNGTNA TGGCTGCGCC CCGNCNCCCG CCAACACCCG
             61 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG
            121 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGA
            181 TCGTCAGTCA GTCACGATGC GGCCGCTCGA GTTAGATCTG GACATAGGAC AAGGTGACAT
            241 CACCCTGGAT TTCCAATGTG TCCACCCTCT GGAAGGCCGA GAGGCGATGG GCAAAGTCAA
            301 AGAGGTGCTG GCCATTGGCG TAAACCTTGA AGCGATCCAA GCCACAGCGA ATGGACAGAT
            361 CAAAGAACTG TCCGGGACCA AATGGGTTGT GGGTGATCTT CTTCTCCTCG GATCCCCACG
            421 AGCCATTCAG AAGGCTGTTC CGGACCACGG TACCGTTGCC CATGCGGGGA TTAATGTGCA
            481 GAGCTATGTC CCCTGAGGAG CCCACCTTGA AGTTGATAGC AAAGCTCTTG CCTGTGGGAG
            541 GCACATAGCC CTTGATGATG ATGGTTCTTC GAGCTGTGAG CCCTCCTTGC AGCCTCCCGA
            601 AATATGGCAC AGGCGGGTTG AAGGTGAGGG GCTGGCCTCC GATGAAGTTG ATTGATTGAA
            661 GTTGCAGATC CCCATCCACT TGCAGGTGGG TGACCATCTG TAGGGGAAGC CGGTGCCCGT
            721 ACTCATAGAA GGGATTTCCA TTTACCACCA CCTTGTAGTG CTCAGCCAGG ACTATGAAGA
            781 CCAGCTCAAA GGCGGCACCC TTTTTGAAGG GCATGCTCCT CTTCCTCTCC TCGCTGCCCC
            841 ACTTCCCGCC CTGCAACGTG TTGAANANCA CCTTGNCCCA GCCGTNANNC GCGGANTGAA
            901 GTGGAAGGCG ANGTCTGANC CNNNATCCTG NCNNACCNCA ANGTTCACGA ANAANNGCTT
            961 CATGNNCTCG CTGGNCNNNN CNTNNNATNN NAACAGANNT TNCCNNNNNN ANCNNNCCNG
          1021 GNNNNGGNNN NNNNNNN
          //
          Primer: M13_-40
          Region: lacI
          Sequence file: RDB18238_B0Gfc.seq check
          >D07204A3_B0Gf_2_M13_-40_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTNNT GGTCNTAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
             61 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
            121 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
            181 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
            241 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT
            301 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT
            361 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA
            421 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA
            481 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT
            541 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT
            601 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG
            661 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGANC CAATGCGACC AGATGCTCCA
            721 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG
            781 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGNATCCT
            841 GNTCATCCAG CGGATAGTTA ATGATCAGCC CACNGACGCG TNGCGCGANA ANANTGTGCA
            901 NNGCCGCTTT ACAGGNTTCN ANGNCGCTTC NTNCNANCAT CGANNNCNNN NCGCTGGNAN
          961 CNANNNGATN NGNNCNANNN TNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.07.27

          GNP_filter3_RDBDEP_html_220727.pl