Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEX-G4Null

Bacteria expression vector of human galectin-4 with a truncated linker peptide (G4Null), GST fusion protein

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18238_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403) Region: GST, thrombin site, Insert 5' Sequence file: RDB18238_B0Gfa.seq
>D07204A3_B0Gf_2_pGEX-F2_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNTTNNANN NNGTATTGNN GCTATCCCNC AAATTGATAA GTACTTGAAA    61 TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC   121 CATCCTCCAA AATCGGATCT GGTTCCGCGT GGATCCCCAG GAATTCCCAT GGCCTATGTC   181 CCCGCACCGG GCTACCAGCC CACCTACAAC CCGACGCTGC CTTACTACCA GCCCATCCCG   241 GGCGGGCTCA ACGTGGGAAT GTCTGTTTAC ATCCAAGGAG TGGCCAGCGA GCACATGAAG   301 CGGTTCTTCG TGAACTTTGT GGTTGGGCAG GATCCGGGCT CAGACGTCGC CTTCCACTTC   361 AATCCGCGGT TTGACGGCTG GGACAAGGTG GTCTTCAACA CGTTGCAGGG CGGGAAGTGG   421 GGCAGCGAGG AGAGGAAGAG GAGCATGCCC TTCAAAAAGG GTGCCGCCTT TGAGCTGGTC   481 TTCATAGTCC TGGCTGAGCA CTACAAGGTG GTGGTAAATG GAAATCCCTT CTATGAGTAC   541 GGGCACCGGC TTCCCCTACA GATGGTCACC CACCTGCAAG TGGATGGGGA TCTGCAACTT   601 CAATCAATCA ACTTCATCGG AGGCCAGCCC CTCACCTTCA ACCCGCCTGT GCCATATTTC   661 GGGAGGCTGC AAGGAGGGCT CACAGCTCGA AGAACCATCA TCATCAAGGG CTATGTGCCT   721 CCCACAGGCA AGAGCTTTGC TATCAACTTC NAGGTGGGCT CCTCANGGGA NATAGCTCTG   781 CACATTAATC CCCGCATGGG CAACGGTACC GNGGTCCGGA ACAGCCTTCT GAATGGCTCG   841 TGGGGATNCG AGGANAANAA GATCACCCAC AACCCNTTTG GNNCCGGANA GTTCTTTGAT   901 CTGNNNNTTC NCTGNGGNTN GNATNGCTTC NAGGTTTACG CNNATGNNCN GCACCNCTTT 961 GANTTTGCCN NNNNNNNNTC NGNNNNNNNN NANGNNNNNN NNNN //
Primer: pJB8-Forward (Pr0084) Region: Insert 3' Sequence file: RDB18238_B0Gfb.seq
>D07204A3_B0Gf_2_pJB8-Forward_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNNGTNA TGGCTGCGCC CCGNCNCCCG CCAACACCCG    61 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG   121 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGA   181 TCGTCAGTCA GTCACGATGC GGCCGCTCGA GTTAGATCTG GACATAGGAC AAGGTGACAT   241 CACCCTGGAT TTCCAATGTG TCCACCCTCT GGAAGGCCGA GAGGCGATGG GCAAAGTCAA   301 AGAGGTGCTG GCCATTGGCG TAAACCTTGA AGCGATCCAA GCCACAGCGA ATGGACAGAT   361 CAAAGAACTG TCCGGGACCA AATGGGTTGT GGGTGATCTT CTTCTCCTCG GATCCCCACG   421 AGCCATTCAG AAGGCTGTTC CGGACCACGG TACCGTTGCC CATGCGGGGA TTAATGTGCA   481 GAGCTATGTC CCCTGAGGAG CCCACCTTGA AGTTGATAGC AAAGCTCTTG CCTGTGGGAG   541 GCACATAGCC CTTGATGATG ATGGTTCTTC GAGCTGTGAG CCCTCCTTGC AGCCTCCCGA   601 AATATGGCAC AGGCGGGTTG AAGGTGAGGG GCTGGCCTCC GATGAAGTTG ATTGATTGAA   661 GTTGCAGATC CCCATCCACT TGCAGGTGGG TGACCATCTG TAGGGGAAGC CGGTGCCCGT   721 ACTCATAGAA GGGATTTCCA TTTACCACCA CCTTGTAGTG CTCAGCCAGG ACTATGAAGA   781 CCAGCTCAAA GGCGGCACCC TTTTTGAAGG GCATGCTCCT CTTCCTCTCC TCGCTGCCCC   841 ACTTCCCGCC CTGCAACGTG TTGAANANCA CCTTGNCCCA GCCGTNANNC GCGGANTGAA   901 GTGGAAGGCG ANGTCTGANC CNNNATCCTG NCNNACCNCA ANGTTCACGA ANAANNGCTT   961 CATGNNCTCG CTGGNCNNNN CNTNNNATNN NAACAGANNT TNCCNNNNNN ANCNNNCCNG 1021 GNNNNGGNNN NNNNNNN //
Primer: M13_-40 (Pr0410) Region: lacI Sequence file: RDB18238_B0Gfc.seq
>D07204A3_B0Gf_2_M13_-40_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNTNNT GGTCNTAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC    61 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT   121 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC   181 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG   241 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT   301 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT   361 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA   421 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA   481 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT   541 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT   601 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG   661 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGANC CAATGCGACC AGATGCTCCA   721 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG   781 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGNATCCT   841 GNTCATCCAG CGGATAGTTA ATGATCAGCC CACNGACGCG TNGCGCGANA ANANTGTGCA   901 NNGCCGCTTT ACAGGNTTCN ANGNCGCTTC NTNCNANCAT CGANNNCNNN NCGCTGGNAN 961 CNANNNGATN NGNNCNANNN TNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl