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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET-mG3-2

Bacterial expression vector of mouse galectin-3 (tag-free protein).

Catalog number RDB19436
Resource name pET-mG3-2
Clone info. Bacterial expression vector of mouse galectin-3 (tag-free protein).
Comment Expression was confirmed by the depositor with SDS-PAGE and Western blotting.
Vector backbone pET-11a (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Ampicillin
Gene/insert name mouse Lgals3 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
mouse Lgals3

          Reference sequence
            
           
          Sequence (full) RDB19436zzt01.seq compiled by us

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.


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          RDB19436 pET-mG3-2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19436_B1Kqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pET-B_F
          Region: T7 pro, lacO, RBS, insert 5'
          Sequence file: RDB19436_B1Kqa.seq check
          >D08453A1_B1Kq_1_pET-B_F_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNCN NNNNNNGCCG NNCNCGATGC GTCCGGCNNN NNNGANCGAG
             61 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT
            121 CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGG CAGACAGCTT
            181 TTCGCTTAAC GATGCCTTAG CTGGCTCTGG AAACCCAAAC CCTCAAGGAT ATCCGGGTGC
            241 ATGGGGGAAC CAGCCTGGGG CAGGGGGCTA CCCAGGGGCT GCCTATCCTG GGGCCTATCC
            301 AGGACAGGCT CCTCCAGGGG CCTACCCAGG ACAGGCTCCT CCAGGGGCCT ATCCAGGACA
            361 GGCTCCTCCT AGTGCCTACC CCGGCCCAAC TGCCCCTGGA GCTTATCCTG GCCCAACTGC
            421 CCCTGGAGCT TATCCTGGCT CAACTGCCCC TGGAGCCTTC CCAGGGCAAC CTGGGGCACC
            481 TGGGGCCTAC CCCAGTGCTC CTGGAGGCTA TCCTGCTGCT GGCCCTTATG GTGTCCCCGC
            541 TGGACCACTG ACGGTGCCCT ATGACCTGCC CTTGCCTGGA GGAGTCATGC CCCGCATGCT
            601 GATCACAATC ATGGGCACAG TGAAACCCAA CGCAAACAGG ATTGTTCTAG ATTTCAGGAG
            661 AGGGAATGAT GTTGCCTTCC ACTTTAACCC CCGCTTCAAT GAGAACAACA GAAGAGTCAT
            721 TGTGTGTAAC ACGAAGCANG ACAATAACTG GGGAAAGGAA GAAAGACAGT CAGCCTTCCC
            781 CTTTGANANT GNAAAACCAT TCAAAATACA AGTCCTGGTT GAAGCTGANC ACTTCAAGGT
            841 TGCGGTCAAC GATGCTCACC TACTGCAGTA CNNNCNNTCN GATGAANAAN CTCCGGGAAN
            901 TCANCNNACT GGGGNTCAGT GNNGACATAA CCCNTCACCA NCGCTAACCN NGCCNTGATC
            961 TAAGGATCCG GCNGCTNACA NNCCCNNAAN NNAANNNNNN NNNGCTGCTG NCNNCNNNTG
           1021 ANNANNANNN NCNNNANNCC NNTNNGGNNN NCTAANNNNN NNNTNNANGG NTTTTNNNNN
          1081 NNNNN
          //
          Primer: pET-check-R1
          Region: T7 ter, insert 3'
          Sequence file: RDB19436_B1Kqb.seq check
          >D08453A1_B1Kq_1_pET-check-R1_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCA GNCNGGCNNC GTGTATGAAA TCTAACAATG
             61 CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC
            121 CGGTACTGCC GGGCCTCTTG CGGGATATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC
            181 CCTCAAGACC CGTTTAGAGG CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC
            241 AGCCAACTCA GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCCTTAGATC ATGGCGTGGT
            301 TAGCGCTGGT GAGGGTTATG TCACCACTGA TCCCCAGTTG GCTGATTTCC CGGAGGTTCT
            361 TCATCCGATG GTTGTACTGC AGTAGGTGAG CATCGTTGAC CGCAACCTTG AAGTGGTCAG
            421 CTTCAACCAG GACTTGTATT TTGAATGGTT TTCCACTCTC AAAGGGGAAG GCTGACTGTC
            481 TTTCTTCCTT TCCCCAGTTA TTGTCCTGCT TCGTGTTACA CACAATGACT CTTCTGTTGT
            541 TCTCATTGAA GCGGGGGTTA AAGTGGAAGG CAACATCATT CCCTCTCCTG AAATCTAGAA
            601 CAATCCTGTT TGCGTTGGGT TTCACTGTGC CCATGATTGT GATCAGCATG CGGGGCATGA
            661 CTCCTCCAGG CAAGGGCAGG TCATAGGGCA CCGTCAGTGG TCCAGCGGGG ACACCATAAG
            721 GGCCAGCAGC AGGATAGCCT CCAGGAGCAC TGGGGTAGGC CCCAGGTGCC CCAGGTTGCC
            781 CTGGGAANGN TCCAGNGGCA GTTGANCCAG GATAAGCTCC AGGGGCAGTT GGGNNAGGAT
            841 AAGCTCCNGG GGCAGTTNGG CCGGGGTANG CACTANGANG ANCCTGTCCN NNNAGGNCCC
            901 NGNANGANCC TGTCCTGGGN ANGNCCCTGG NNGANCCTGN CCNNNNNANN NCCCNNNNAT
            961 ANGNANCCCC TGGGNAGCCC CNNGNCNCAN NNNNNNTNNC CCNNNGNNNC CGGNNNTNCN
          1021 TNNNNGNNTN NGNNTNNNNN ANNNNNNNNN N
          //
          Primer: lacIpro_F
          Region: lacI pro, lacI
          Sequence file: RDB19436_B1Kqc.seq check
          >D08453A1_B1Kq_1_lacIpro_F_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNAN ANTCAATTCN GGGTGGTGAA TGTGAAACCA
             61 GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG
            121 GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG
            181 GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG
            241 ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT
            301 AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC
            361 GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC
            421 ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT
            481 CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT
            541 GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCANNA GCAAATCGCG
            601 CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC NTCTGGCTGG CTGGCATNNT
            661 ATCTCACTCG CAATCAAATT CNGCCNANAG CGGANCGGGN NNGNGANNNN NNNGNNNNGN
          721 NNNGNNTTNN NNNNANNNNN NNNANNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl