RDB19436 - pET-mG3-2

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pET-mG3-2 (#RDB19436)

Bacterial expression vector of mouse galectin-3 (tag-free protein).

Clone info.	Bacterial expression vector of mouse galectin-3 (tag-free protein).
Comment	Expression was confirmed by the depositor with SDS-PAGE and Western blotting.
Vector backbone	pET-11a (plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Ampicillin
Gene/insert name	mouse Lgals3 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Sequence (full)	RDB19436zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、学術機関の学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19436	pET-mG3-2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-mG3-2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19436).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19436_B1Kqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, RBS, insert 5' Sequence file: RDB19436_B1Kqa.seq
>D08453A1_B1Kq_1_pET-B_F_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNCN NNNNNNGCCG NNCNCGATGC GTCCGGCNNN NNNGANCGAG 61 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT 121 CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGG CAGACAGCTT 181 TTCGCTTAAC GATGCCTTAG CTGGCTCTGG AAACCCAAAC CCTCAAGGAT ATCCGGGTGC 241 ATGGGGGAAC CAGCCTGGGG CAGGGGGCTA CCCAGGGGCT GCCTATCCTG GGGCCTATCC 301 AGGACAGGCT CCTCCAGGGG CCTACCCAGG ACAGGCTCCT CCAGGGGCCT ATCCAGGACA 361 GGCTCCTCCT AGTGCCTACC CCGGCCCAAC TGCCCCTGGA GCTTATCCTG GCCCAACTGC 421 CCCTGGAGCT TATCCTGGCT CAACTGCCCC TGGAGCCTTC CCAGGGCAAC CTGGGGCACC 481 TGGGGCCTAC CCCAGTGCTC CTGGAGGCTA TCCTGCTGCT GGCCCTTATG GTGTCCCCGC 541 TGGACCACTG ACGGTGCCCT ATGACCTGCC CTTGCCTGGA GGAGTCATGC CCCGCATGCT 601 GATCACAATC ATGGGCACAG TGAAACCCAA CGCAAACAGG ATTGTTCTAG ATTTCAGGAG 661 AGGGAATGAT GTTGCCTTCC ACTTTAACCC CCGCTTCAAT GAGAACAACA GAAGAGTCAT 721 TGTGTGTAAC ACGAAGCANG ACAATAACTG GGGAAAGGAA GAAAGACAGT CAGCCTTCCC 781 CTTTGANANT GNAAAACCAT TCAAAATACA AGTCCTGGTT GAAGCTGANC ACTTCAAGGT 841 TGCGGTCAAC GATGCTCACC TACTGCAGTA CNNNCNNTCN GATGAANAAN CTCCGGGAAN 901 TCANCNNACT GGGGNTCAGT GNNGACATAA CCCNTCACCA NCGCTAACCN NGCCNTGATC 961 TAAGGATCCG GCNGCTNACA NNCCCNNAAN NNAANNNNNN NNNGCTGCTG NCNNCNNNTG 1021 ANNANNANNN NCNNNANNCC NNTNNGGNNN NCTAANNNNN NNNTNNANGG NTTTTNNNNN 1081 NNNNN //
Primer: pET-check-R1 (Pr0192) Region: T7 ter, insert 3' Sequence file: RDB19436_B1Kqb.seq
>D08453A1_B1Kq_1_pET-check-R1_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCA GNCNGGCNNC GTGTATGAAA TCTAACAATG 61 CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC 121 CGGTACTGCC GGGCCTCTTG CGGGATATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC 181 CCTCAAGACC CGTTTAGAGG CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC 241 AGCCAACTCA GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCCTTAGATC ATGGCGTGGT 301 TAGCGCTGGT GAGGGTTATG TCACCACTGA TCCCCAGTTG GCTGATTTCC CGGAGGTTCT 361 TCATCCGATG GTTGTACTGC AGTAGGTGAG CATCGTTGAC CGCAACCTTG AAGTGGTCAG 421 CTTCAACCAG GACTTGTATT TTGAATGGTT TTCCACTCTC AAAGGGGAAG GCTGACTGTC 481 TTTCTTCCTT TCCCCAGTTA TTGTCCTGCT TCGTGTTACA CACAATGACT CTTCTGTTGT 541 TCTCATTGAA GCGGGGGTTA AAGTGGAAGG CAACATCATT CCCTCTCCTG AAATCTAGAA 601 CAATCCTGTT TGCGTTGGGT TTCACTGTGC CCATGATTGT GATCAGCATG CGGGGCATGA 661 CTCCTCCAGG CAAGGGCAGG TCATAGGGCA CCGTCAGTGG TCCAGCGGGG ACACCATAAG 721 GGCCAGCAGC AGGATAGCCT CCAGGAGCAC TGGGGTAGGC CCCAGGTGCC CCAGGTTGCC 781 CTGGGAANGN TCCAGNGGCA GTTGANCCAG GATAAGCTCC AGGGGCAGTT GGGNNAGGAT 841 AAGCTCCNGG GGCAGTTNGG CCGGGGTANG CACTANGANG ANCCTGTCCN NNNAGGNCCC 901 NGNANGANCC TGTCCTGGGN ANGNCCCTGG NNGANCCTGN CCNNNNNANN NCCCNNNNAT 961 ANGNANCCCC TGGGNAGCCC CNNGNCNCAN NNNNNNTNNC CCNNNGNNNC CGGNNNTNCN 1021 TNNNNGNNTN NGNNTNNNNN ANNNNNNNNN N //
Primer: lacIpro_F (Pr0409) Region: lacI pro, lacI Sequence file: RDB19436_B1Kqc.seq
>D08453A1_B1Kq_1_lacIpro_F_A03_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNAN ANTCAATTCN GGGTGGTGAA TGTGAAACCA 61 GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG 121 GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG 181 GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG 241 ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT 301 AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC 361 GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC 421 ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT 481 CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT 541 GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCANNA GCAAATCGCG 601 CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC NTCTGGCTGG CTGGCATNNT 661 ATCTCACTCG CAATCAAATT CNGCCNANAG CGGANCGGGN NNGNGANNNN NNNGNNNNGN 721 NNNGNNTTNN NNNNANNNNN NNNANNNNNN NNNN //