Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET-mG3-2

Bacterial expression vector of mouse galectin-3 (tag-free protein).

Sequence (full)	RDB19436zzt01.seq compiled by us

Distribution information

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19436_B1Kqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, RBS, insert 5' Sequence file: RDB19436_B1Kqa.seq
>D08453A1_B1Kq_1_pET-B_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNCN NNNNNNGCCG NNCNCGATGC GTCCGGCNNN NNNGANCGAG    61 ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT   121 CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGG CAGACAGCTT   181 TTCGCTTAAC GATGCCTTAG CTGGCTCTGG AAACCCAAAC CCTCAAGGAT ATCCGGGTGC   241 ATGGGGGAAC CAGCCTGGGG CAGGGGGCTA CCCAGGGGCT GCCTATCCTG GGGCCTATCC   301 AGGACAGGCT CCTCCAGGGG CCTACCCAGG ACAGGCTCCT CCAGGGGCCT ATCCAGGACA   361 GGCTCCTCCT AGTGCCTACC CCGGCCCAAC TGCCCCTGGA GCTTATCCTG GCCCAACTGC   421 CCCTGGAGCT TATCCTGGCT CAACTGCCCC TGGAGCCTTC CCAGGGCAAC CTGGGGCACC   481 TGGGGCCTAC CCCAGTGCTC CTGGAGGCTA TCCTGCTGCT GGCCCTTATG GTGTCCCCGC   541 TGGACCACTG ACGGTGCCCT ATGACCTGCC CTTGCCTGGA GGAGTCATGC CCCGCATGCT   601 GATCACAATC ATGGGCACAG TGAAACCCAA CGCAAACAGG ATTGTTCTAG ATTTCAGGAG   661 AGGGAATGAT GTTGCCTTCC ACTTTAACCC CCGCTTCAAT GAGAACAACA GAAGAGTCAT   721 TGTGTGTAAC ACGAAGCANG ACAATAACTG GGGAAAGGAA GAAAGACAGT CAGCCTTCCC   781 CTTTGANANT GNAAAACCAT TCAAAATACA AGTCCTGGTT GAAGCTGANC ACTTCAAGGT   841 TGCGGTCAAC GATGCTCACC TACTGCAGTA CNNNCNNTCN GATGAANAAN CTCCGGGAAN   901 TCANCNNACT GGGGNTCAGT GNNGACATAA CCCNTCACCA NCGCTAACCN NGCCNTGATC   961 TAAGGATCCG GCNGCTNACA NNCCCNNAAN NNAANNNNNN NNNGCTGCTG NCNNCNNNTG  1021 ANNANNANNN NCNNNANNCC NNTNNGGNNN NCTAANNNNN NNNTNNANGG NTTTTNNNNN 1081 NNNNN //
Primer: pET-check-R1 (Pr0192) Region: T7 ter, insert 3' Sequence file: RDB19436_B1Kqb.seq
>D08453A1_B1Kq_1_pET-check-R1_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCA GNCNGGCNNC GTGTATGAAA TCTAACAATG    61 CGCTCATCGT CATCCTCGGC ACCGTCACCC TGGATGCTGT AGGCATAGGC TTGGTTATGC   121 CGGTACTGCC GGGCCTCTTG CGGGATATCC GGATATAGTT CCTCCTTTCA GCAAAAAACC   181 CCTCAAGACC CGTTTAGAGG CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC   241 AGCCAACTCA GCTTCCTTTC GGGCTTTGTT AGCAGCCGGA TCCTTAGATC ATGGCGTGGT   301 TAGCGCTGGT GAGGGTTATG TCACCACTGA TCCCCAGTTG GCTGATTTCC CGGAGGTTCT   361 TCATCCGATG GTTGTACTGC AGTAGGTGAG CATCGTTGAC CGCAACCTTG AAGTGGTCAG   421 CTTCAACCAG GACTTGTATT TTGAATGGTT TTCCACTCTC AAAGGGGAAG GCTGACTGTC   481 TTTCTTCCTT TCCCCAGTTA TTGTCCTGCT TCGTGTTACA CACAATGACT CTTCTGTTGT   541 TCTCATTGAA GCGGGGGTTA AAGTGGAAGG CAACATCATT CCCTCTCCTG AAATCTAGAA   601 CAATCCTGTT TGCGTTGGGT TTCACTGTGC CCATGATTGT GATCAGCATG CGGGGCATGA   661 CTCCTCCAGG CAAGGGCAGG TCATAGGGCA CCGTCAGTGG TCCAGCGGGG ACACCATAAG   721 GGCCAGCAGC AGGATAGCCT CCAGGAGCAC TGGGGTAGGC CCCAGGTGCC CCAGGTTGCC   781 CTGGGAANGN TCCAGNGGCA GTTGANCCAG GATAAGCTCC AGGGGCAGTT GGGNNAGGAT   841 AAGCTCCNGG GGCAGTTNGG CCGGGGTANG CACTANGANG ANCCTGTCCN NNNAGGNCCC   901 NGNANGANCC TGTCCTGGGN ANGNCCCTGG NNGANCCTGN CCNNNNNANN NCCCNNNNAT   961 ANGNANCCCC TGGGNAGCCC CNNGNCNCAN NNNNNNTNNC CCNNNGNNNC CGGNNNTNCN 1021 TNNNNGNNTN NGNNTNNNNN ANNNNNNNNN N //
Primer: lacIpro_F (Pr0409) Region: lacI pro, lacI Sequence file: RDB19436_B1Kqc.seq
>D08453A1_B1Kq_1_lacIpro_F_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNAN ANTCAATTCN GGGTGGTGAA TGTGAAACCA    61 GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG   121 GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG   181 GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG   241 ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT   301 AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC   361 GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC   421 ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT   481 CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT   541 GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCANNA GCAAATCGCG   601 CTGTTAGCGG GCCCATTAAG TTCTGTCTCG GCGCGTCTGC NTCTGGCTGG CTGGCATNNT   661 ATCTCACTCG CAATCAAATT CNGCCNANAG CGGANCGGGN NNGNGANNNN NNNGNNNNGN 721 NNNGNNTTNN NNNNANNNNN NNNANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl