RDB08416 - pET-G9(S)-3a

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pET-G9(S)-3a

Bacteria expression vector of human galectin-9 with a short linker peptide (G9(S)/G9S), tag-free protein

Catalog number	RDB08416
Resource name	pET-G9(S)-3a
Clone info.	Bacterial expression clone of human galectin-9 (short linker type).
Comment	Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone	pET-11a (Plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	Human galectin-9 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Other clones in our bank

human galectin-9

External Database
Human galectin-9

Reference sequence

Distribution information

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Terms and conditions set forth by the DEPOSITOR	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	本件リソースの利用は、学術機関の学術研究に限る。
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RDB08416	pET-G9(S)-3a	DNA solution

Please review the QC test results indicated by check

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References and tips

Electronic file

Sequence	RDB08416z.seq

Original reference

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08416_A4Ek.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08416_A4Eka.seq
>08416_08416_A4Ek_T7_20bp_G04_19.ab1 1 GCGGAACAGT TCCCTCTAGA ATAATTTTGT TTAACTTTAA GAAGGAGATA TACATATGGC 61 CTTCAGCGGT TCCCAGGCTC CCTACCTGAG TCCAGCTGTC CCCTTTTCTG GGACTATTCA 121 AGGAGGTCTC CAGGACGGAC TTCAGATCAC TGTCAATGGG ACCGTTCTCA GCTCCAGTGG 181 AACCAGGTTT GCTGTGAACT TTCAGACTGG CTTCAGTGGA AATGACATTG CCTTCCACTT 241 CAACCCTCGG TTTGAAGATG GAGGGTACGT GGTGTGCAAC ACGAGGCAGA ACGGAAGCTG 301 GGGGCCCGAG GAGAGGAAGA CACACATGCC TTTCCAGAAG GGGATGCCCT TTGACCTCTG 361 CTTCCTGGTG CAGAGCTCAG ATTTCAAGGT GATGGTGAAC GGGATCCTCT TCGTGCAGTA 421 CTTCCACCGC GTGCCCTTCC ACCGTGTGGA CACCATCTCC GTCAATGGCT CTGTGCAGCT 481 GTCCTACATC AGCTTCCAGA CCCAGACAGT CATCCACACA GTGCAGAGCG CCCCTGGACA 541 GATGTTCTCT ACTCCCGCCA TCCCACCTAT GATGTACCCC CACCCCGCCT ATCCGATGCC 601 TTTCATCACC ACCATTCTGG GAGGGCTGTA CCCATCCAAG TCCATCCTCC TGTCAGGCAC 661 TGTCCTGCCC AGTGCTCAGA GGTTCCACAT CAACCTGTGC TCTGGGAACC ACATCGCCTT 721 CCACCTGAAC CCCCGTTTTG ATGAGAATGC TGTGGTCCGC AACACCCAGA TCGACAACTC 781 CTGGGGGTCT GAGGAGCGAA GTCTGCCCCG AAAAATGCCC TTCGTCCGTG GCCAGAGCTT 841 CTCAGTGTGG ATCTTGTGTG AAGCTCACTG CCTCAAGGTG GCCGTGGATG GTCAGCACCT 901 GTTTGAATAC TACCATCGCC TGAGGAACCT GCCCACCATC AACAGACTGG AAGTGGGGGG 961 CGACATCCAG CTGACCCATG TGCAGACATA GAGATCCCGG CTGCTAACAA AGCCCGAAAG 1021 GAAGCTGAGT TGGCTGCTGC CACGCTGAGC ATACTAGCAT AACCCCTTGG GGCCTCTAAA 1081 CGGGTCTGAG GGTTTTTGCT GGAATGAGAC TATATCAGAT ATCCCGCAGA GGCGGCAGTA 1141 CGGCATACCA GCCTATGCCT TACAGCCATC AGGTTGAACG GGGTGGTCCG AGGA //
Primer: T7_terminator Sequence file: RDB08416_A4Ekb.seq
>08416_08416_A4Ek_T7terminator_H04_22.ab1 1 AATTTCGCCT TCCTTTTCGG GCTTTGTTAG CAGCCGGATC TCTATGTCTG CACATGGGTC 61 AGCTGGATGT CGCCCCCCAC TTCCAGTCTG TTGATGGTGG GCAGGTTCCT CAGGCGATGG 121 TAGTATTCAA ACAGGTGCTG ACCATCCACG GCCACCTTGA GGCAGTGAGC TTCACACAAG 181 ATCCACACTG AGAAGCTCTG GCCACGGACG AAGGGCATTT TTCGGGGCAG ACTTCGCTCC 241 TCAGACCCCC AGGAGTTGTC GATCTGGGTG TTGCGGACCA CAGCATTCTC ATCAAAACGG 301 GGGTTCAGGT GGAAGGCGAT GTGGTTCCCA GAGCACAGGT TGATGTGGAA CCTCTGAGCA 361 CTGGGCAGGA CAGTGCCTGA CAGGAGGATG GACTTGGATG GGTACAGCCC TCCCAGAATG 421 GTGGTGATGA AAGGCATCGG ATAGGCGGGG TGGGGGTACA TCATAGGTGG GATGGCGGGA 481 GTAGAGAACA TCTGTCCAGG GGCGCTCTGC ACTGTGTGGA TGACTGTCTG GGTCTGGAAG 541 CTGATGTAGG ACAGCTGCAC AGAGCCATTG ACGGAGATGG TGTCCACACG GTGGAAGGGC 601 ACGCGGTGGA AGTACTGCAC GAAGAGGATC CCGTTCACCA TCACCTTGAA ATCTGAGCTC 661 TGCACCAGGA AGCAGAGGTC AAAGGGCATC CCCTTCTGGA AAGGCATGTG TGTCTTCCTC 721 TCCTCGGGCC CCCAGCTTCC GTTCTGCCTC GTGTTGCACA CCACGTACCC TCCATCTTCA 781 AACCGAGGGT TGAAGTGGAA GGCAATGTCA TTTCCACTGA AGCCAGTCTG AAAGTTCACA 841 GCAAACCTGG TTCCACTGGA GCTGAGAACG GTCCCATTGA CAGTGATCTG AAGTCCGTCC 901 TGAGACCTCC TTGAATAGTC CCAGAAAAGG GGACAGCTGG ACTCAGTAGG GAGCCTGGGA 961 ACCGCTGAAG GCCATATGTA TATCTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG 1021 GAATTGTTAT CCGCTCACAA TTCCCCTATA GTGAGTCGTA TTAATTTCGC GGGATCGAGA 1081 TCTCGATCCT CTACGCCGGG ACGCATCGTG GCCGGCATCA CCGGGCGCCC ACAGGTGCGA 1141 TGGCTGCGGC TATTATCGTC GAACATCCAC GAATTGGGAA GGATCCGGGC TCCGCCACTT 1201 CCCGGCCTTC AATTGAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol	Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

2022.05.17

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