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pGEX-G8Null-1a (#RDB08454)

Bacteria expression vector of human galectin-8 with a truncated linker peptide (G8Null), GST fusion protein

Alternative name pGEX-G8NC(Null)-1a
Clone info. Bacterial expression clone of human galectin-8 with a truncated linker peptide.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-8 cDNA
Depositor|Developer Nishi, Nozomu |
 
Sequence RDB08454z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 本件リソースの利用は、学術機関の学術研究に限る。

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RDB08454 pGEX-G8Null-1a Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pGEX-G8Null-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08454).

Reference section:

Nishi, N., Itoh, A., Fujiyama, A., Yoshida, N., Araya, S., Hirashima, M., Shoji, H., Nakamura, T., Development of highly stable galectins: truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins. FEBS Lett. 579: 2058-2064 (2005). PMID 15811318. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nishi, N., Development of highly stable galectins: truncation of the linker peptide confers protease-resistance on tandem-repeat type galectins. FEBS Lett. 579: 2058-2064 (2005). PMID 15811318. [link to RRC of NBRP]
protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]