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pGEX-CSG1-2a (#RDB08448)

Bacteria expression vector of human galectin-1 in which all Cys residues are substituted with Ser residues (G1C3,17,43,61,89,131S: CSG1), GST fusion protein


Drawn by SnapGene® software
Sequence (full) RDB08448hts01.seq
Publication Nishi, N., Glycobiology 18: 1065-1073 (2008). [link to RRC of NBRP]
Test sheet RDB08448_A2Crp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Bacterial expression clone of human galectin-1 with C3,17,43,61,89,131S mutations.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-1 cDNA
Depositor|Developer Nishi, Nozomu |

Distribution information

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Terms and conditions for distribution The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
提供案内 (日本国内) [open/close]

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RDB08448 pGEX-CSG1-2a DNA solution

How to cite this biological resource

Materials & Methods section:

The pGEX-CSG1-2a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08448).

Reference section:

Nishi, N., Abe, A., Iwaki, J., Yoshida, H., Itoh, A., Shoji, H., Kamitori, S., Hirabayashi, J., Nakamura, T., Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1. Glycobiology 18: 1065-1073 (2008). PMID 18796645. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nishi, N., Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1. Glycobiology 18: 1065-1073 (2008). PMID 18796645. [link to RRC of NBRP]
protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]