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pTrc-G4-1a

Bacteria expression vector of human galectin-4, (His) 6 -containing-tag protein

Catalog number RDB04220
Resource name pTrc-G4-1a
Clone info. Expression vector of human galectin-4.
Vector backbone pTrcHis B (Plasmid)
Size of vector backbone 4.4 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 4 cDNA
Depositor|Developer Nishi, Nozomu |
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External Database
Human lectin, galactoside-binding, soluble, 4

          Reference sequence
            

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          RDB04220 pTrc-G4-1a DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB04220.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13755_A6A4p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Reverse2
          Sequence file: RDB13755_A6A4a.seq check
          >04220_13755_A6A4_Reverse2_E08_14.ab1
              1 CCCCGTGAAA GCGAGCGGCA CTGCTCTTTA CAATTTATCA GACAATCTGT GTGGGCACTC
             61 GACCGGAATT ATCGATTAAC TTTATTATTA AAAATTAAAG AGGTATATAT TAATGTATCG
            121 ATTAAATAAG GAGGAATAAA CCATGGGGGG TTCTCATCAT CATCATCATC ATGGTATGGC
            181 TAGCATGACT GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCC
            241 GAGCTCGAGA ATGGCCTATG TCCCCGCACC GGGCTACCAG CCCACCTACA ACCCGACGCT
            301 GCCTTACTAC CAGCCCATCC CGGGCGGGCT CAACGTGGGA ATGTCTGTTT ACATCCAAGG
            361 AGTGGCCAGC GAGCACATGA AGCGGTTCTT CGTGAACTTT GTGGTTGGGC AGGATCCGGG
            421 CTCAGACGTC GCCTTCCACT TCAATCCGCG GTTTGACGGC TGGGACAAGG TGGTCTTCAA
            481 CACGTTGCAG GGCGGGAAGT GGGGCAGCGA GGAGAGGAAG AGGAGCATGC CCTTCAAAAA
            541 GGGTGCCGCC TTTGAGCTGG TCTTCATAGT CCTGGCTGAG CACTACAAGG TGGTGGTAAA
            601 TGGAAATCCC TTCTATGAGT ACGGGCACCG GCTTCCCCTA CAGATGGTCA CCCACCTGCA
            661 AGTGGATGGG GATCTGCAAC TTCAATCAAT CAACTTCATC GGAGGCCAGC CCCTCCGGCC
            721 CCAGGGACCC CCGATGATGC CACCTTACCC TGGTCCCGGA CATTGCCATC AACAGCTGAA
            781 CAGCCTGCCC ACCATGGAAG GACCCCCCAA CCTTCAACCC GCCTGTGCCA TATTTCGGGA
            841 GGCTGCAAAG GAGGGCTCAC AGCTCGAAAG AACCCATCAT CATCAAAGGC TATGTGCCCT
            901 CCCACAGGCA AGAGCTTTGC TATCAACTTC AAAGTGGGCT CCTCACGGGA CATAGCTCTG
            961 CACATTAATC CCCGCATGGG CACGGGTACC GTTGGCTCAG ACCAGTCGTT CCTGAATGGC
          1021 TTCGTTGGGA ATCGAAGAGA GAAGATTCAG CGACACCCAT TTTGGATTTC
          //
          Primer: pTrcHis-R
          Sequence file: RDB13755_A6A4b.seq check
          >04220_13755_A6A4_pTrcHis-R_F08_17.ab1
              1 GGGCCGGCCG TATCTTCTCT CATCCGCCAA CAGCCAAGCT TCGAATTCTT AGATCTGGAC
             61 ATAGGACAAG GTGACATCAC CCTGGATTTC CAATGTGTCC ACCCTCTGGA AGGCCGAGAG
            121 GCGATGGGCA AAGTCAAAGA GGTGCTGGCC ATTGGCGTAA ACCTTGAAGC GATCCAAGCC
            181 ACAGCGAATG GACAGATCAA AGAACTGTCC GGGACCAAAT GGGTTGTGGG TGATCTTCTT
            241 CTCCTCGGAT CCCCACGAGC CATTCAGAAG GCTGTTCCGG ACCACGGTAC CGTTGCCCAT
            301 GCGGGGATTA ATGTGCAGAG CTATGTCCCC TGAGGAGCCC ACCTTGAAGT TGATAGCAAA
            361 GCTCTTGCCT GTGGGAGGCA CATAGCCCTT GATGATGATG GTTCTTCGAG CTGTGAGCCC
            421 TCCTTGCAGC CTCCCGAAAT ATGGCACAGG CGGGTTGAAG GTTGGGGGTC CTTCCATGGT
            481 GGGCAGGCTG TTCAGCTGTT GATGGCAATG TCCGGGACCA GGGTAAGGTG GCATCATCGG
            541 GGGTCCCTGG GGCCGGAGGG GCTGGCCTCC GATGAAGTTG ATTGATTGAA GTTGCAGATC
            601 CCCATCCACT TGCAGGTGGG TGACCATCTG TAGGGGAAGC CGGTGCCCGT ACTCATAGAA
            661 GGGATTTCCA TTTACCACCA CCTTGTAGTG CTCAGCCAGG ACTATGAAGA CCAGCTCAAA
            721 GGCGGCACCC TTTTTGAAGG GCATGCTCCT CTTCCTCTCC TCGCTGCCCC ACTTCCCGCC
            781 CTGCAACGTG TTGAAGACCA CCTTGTCCCA GCCGTCAAAC CGCGGATTGA AGTGGAAGGC
            841 GACGTCTGAG CCCGGATCCT GCCCAACCAC AAAGTTCACG AAAGAACCGC TTCATGTGCT
            901 CGCTGGCCAC TCCCTTGGAT GTAAACAGAC ATTTCCCACG TTTGAGCCCG CCCGGGATGG
            961 GCTGGTAGTA AAGCAGCGTC CGGATGTAGG TGGGCTGGTA GCCCGGTGCG GGGACATAGC
           1021 CCATTCTCGA GCTTCGAATT CGTATCGTTC ATCGATCGTA CAGGATTCCC GATCGATTAG
          1081 CTGTCCACCA GTTCAAT
          //
          Primer: pGEX-R
          Sequence file: RDB13755_A6A4c.seq check
          >04220_13755_A6A4_pGEX-R_G08_20.ab1
              1 TTGGATTAGG ACGCGCGAGC AGCAGATCAA TTCGCGCGCG AAGGCGAAGC GGCATGCATT
             61 TACGTTGACA CCATCGAATG GTGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA
            121 GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT
            181 GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG
            241 AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG
            301 GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC
            361 CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC
            421 AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC
            481 AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT
            541 GCCATTGCTG TGGAAGCTGC CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC
            601 CAGACACCCA TCAACAGTAT TATTTTCTCC CATGAAGACG GTACGCGACT GGGCGTGGAG
            661 CATCTGGTCG CATTGGGTCA CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC
            721 TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG
            781 ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG
            841 CTGAATGAGG GCATCGTTCC CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC
            901 GCAATGCGCG CCATTACCGA GTCCCGGGCT GCGCGTTTGG GTGCGGATAT CTCGGGTAGT
            961 GGATACGACG ATACCCGAGA CAGCTCATGT ATATCCCGGC CGGTAACAAC AATCAACAGA
          1021 TTTTCGCTTG CCTGGGCAAC CAGCGATGAC CGCTTGGCCT GCCACTCCTT CTCCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl