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pGEX-G8(M)-2a

Bacteria expression vector of human galectin-8 (the predominant isoform of G8 in most cell and tissue types, G8(M)/G8M), GST fusion protein

Catalog number RDB04222
Resource name pGEX-G8(M)-2a
Clone info. Expression vector of human galectin-8.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-8 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Human galectin-8

          Reference sequence
            

          Distribution information

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          RDB04222 pGEX-G8(M)-2a DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB04222.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13058_A5Ad.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX_F
          Sequence file: RDB13058_A5Ada.seq check
          >04222_13058_A5Ad_pGEX-F_G08_20.ab1
              1 GTCATCGTCA ATCGGATCTG GTTCCGCGTG GATCTATGAT GTTGTCCTTA AACAACCTAC
             61 AGAATATCAT CTATAACCCG GTAATCCCGT TTGTTGGCAC CATTCCTGAT CAGCTGGATC
            121 CTGGAACTTT GATTGTGATA CGTGGGCATG TTCCTAGTGA CGCAGACAGA TTCCAGGTGG
            181 ATCTGCAGAA TGGCAGCAGC ATGAAACCTC GAGCCGATGT GGCCTTTCAT TTCAATCCTC
            241 GTTTCAAAAG GGCCGGCTGC ATTGTTTGCA ATACTTTGAT AAATGAAAAA TGGGGACGGG
            301 AAGAGATCAC CTATGACACG CCTTTCAAAA GAGAAAAGTC TTTTGAGATC GTGATTATGG
            361 TGCTGAAGGA CAAATTCCAG GTGGCTGTAA ATGGAAAACA TACTCTGCTC TATGGCCACA
            421 GGATCGGCCC AGAGAAAATA GACACTCTGG GCATTTATGG CAAAGTGAAT ATTCACTCAA
            481 TTGGTTTTAG CTTCAGCTCG GACTTACAAA GTACCCAAGC ATCTAGTCTG GAACTGACAG
            541 AGATAAGTAG AGAAAATGTT CCAAAGTCTG GCACGCCCCA GCTTAGGCTG CCATTCGCTG
            601 CAAGGTTGAA CACCCCCATG GGCCCTGGAC GAACTGTCGT CGTTAAAGGA GAAGTGAATG
            661 CAAATGCCAA AAGCTTTAAT GTTGACCTAC TAGCAGGAAA ATCAAAGGAT ATTGCTCTAC
            721 ACTTGAACCC ACGCCTGAAT ATTAAAGCAT TTGTAAGAAA TTCTTTTCTT CAGGAGTCCT
            781 GGGGGAGAAG AAGAGAGAAA TATTACCTCT TTCCCATTTA GTCCTGGGAT GTACTTTGAG
            841 ATGATAATTT ATTGTGATGT TAGAGAATTC AAGGTTGCAG TAAATGGCGT ACACAGCCTG
            901 GAGTACAAAC ACAGATTTAA AGAGCTCAGC AGTATTGACA CGCTGGAAAT TAATGGAGAC
            961 ATCCACTTAC TGGAAAGTAA GGAGCTGGTA GGTCGAGCGG CCGCATCGTG ACTGACTGAC
           1021 GATCTGCCTC GCGCGTTTTC GGTGATGACG GTGAAAAACC CTCTGGACAC ATGGCCAGGC
          1081 TCCCGGGAAG AACGGATCCA CACAGGCCTT TGATCCTTGA TTCG
          //
          Primer: pGEX_R
          Sequence file: RDB13058_A5Adb.seq check
          >04222_13058_A5Ad_pGEX-R_H08_23.ab1
              1 TTCATCGGCA ACGCGCGAGG CAGATCGTCA GTCAGTCACG ATGCGGCCGC TCGACCTACC
             61 AGCTCCTTAC TTCCAGTAAG TGGATGTCTC CATTAATTTC CAGCGTGTCA ATACTGCTGA
            121 GCTCTTTAAA TCTGTGTTTG TACTCCAGGC TGTGTACGCC ATTTACTGCA ACCTTGAATT
            181 CTCTAACATC ACAATAAATT ATCATCTCAA AGTACATCCC AGGACTAAAT GGGAAAGAGG
            241 TAATATTTCT CTCTTCTTCT CCCCAGGACT CCTGAAGAAA AGAATTTCTT ACAAATGCTT
            301 TAATATTCAG GCGTGGGTTC AAGTGTAGAG CAATATCCTT TGATTTTCCT GCTAGTAGGT
            361 CAACATTAAA GCTTTTGGCA TTTGCATTCA CTTCTCCTTT AACGACGACA GTTCGTCCAG
            421 GGCCCATGGG GGTGTTCAAC CTTGCAGCGA ATGGCAGCCT AAGCTGGGGC GTGCCAGACT
            481 TTGGAACATT TTCTCTACTT ATCTCTGTCA GTTCCAGACT AGATGCTTGG GTACTTTGTA
            541 AGTCCGAGCT GAAGCTAAAA CCAATTGAGT GAATATTCAC TTTGCCATAA ATGCCCAGAG
            601 TGTCTATTTT CTCTGGGCCG ATCCTGTGGC CATAGAGCAG AGTATGTTTT CCATTTACAG
            661 CCACCTGGAA TTTGTCCTTC AGCACCATAA TCACGATCTC AAAAGACTTT TCTCTTTTGA
            721 AAGGCGTGTC ATAGGTGATC TCTTCCCGTC CCCATTTTTC ATTTATCAAA GTATTGCAAA
            781 CAATGCAGCC GGCCCTTTTG AAACGAGGAT TGAAATGAAA GGCCACATCG GCTCGAGGTT
            841 TCATGCTGCT GCCATTCTGC AGATCCACCT GGAATCTGTC TGCGTCACTA GGAACATGCC
            901 CACGTATCAC AATCAAAGTT CCAGGATCCA GCTGATCAGG AATGGTGCCC AACAAACGGG
            961 ATTACCGGGT TATAGATGAT ATTTCTGTAG GTTGTTTAAA GGACAACATC ATAGATCCAC
           1021 GCGGGAACCC AGATCCGATT TTGAAGATGG TCGCACCACC AAACGTGCTG CAGCCCTGCA
          1081 AGGCCATGCT ATATACTGGC CTGGAATTCC AGTACTTATT CAATTTTTGT T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl