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pET-ssG9 (#RDB15282)

Bacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null) with a 10-amino acid deletion and a single amino acid mutation at the remaining linker peptide (ssG9), tag-free protein


Drawn by SnapGene® software
Sequence (full) RDB15282hts02.seq
Publication Itoh, A., Glycobiology 23 (8): 920-925 (2013). [link to RRC of NBRP]
Test sheet RDB15282_B0J6p1-1.pdf 
Assembled from experimentally sequenced data.
 
Alternative name pET-G9Null-CCRD-mC10-HPPY-1a
Clone info. Expression vector of mutant human galectin-9 [10-amino acid deletion with a single amino acid substitution at the linker region of G9Null], see Fig1 and TableS1 of Itoh, A. et al., Glycobiology, 23 (8): 920-925, 2013.
Comment Expression was confirmed by the depositor with SDS-PAGE.
Vector backbone pET-11a (Plasmid)
Selectable markers Amp^r
Gene/insert name Human LGALS9 cDNA
Depositor|Developer Nishi, Nozomu |

Distribution information

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Terms and conditions for distribution The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
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RDB15282 pET-ssG9 DNA solution

How to cite this biological resource

Materials & Methods section:

The pET-ssG9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15282).

Reference section:

Itoh, A., Fukata, Y., Miyanaka, H., Nonaka, Y., Ogawa, T., Nakamura, T., Nishi, N., Optimization of the inter-domain structure of galectin-9 for recombinant production. Glycobiology 23 (8): 920-925 (2013). PMID 23507964. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Itoh, A., Optimization of the inter-domain structure of galectin-9 for recombinant production. Glycobiology 23 (8): 920-925 (2013). PMID 23507964. [link to RRC of NBRP]
protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]