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pET-ssG9

Bacteria expression vector of human galectin-9 with a truncated linker peptide (G9Null) with a 10-amino acid deletion and a single amino acid mutation at the remaining linker peptide (ssG9), tag-free protein

Catalog number RDB15282
Resource name pET-ssG9
Alternative name pET-G9Null-CCRD-mC10-HPPY-1a
Clone info. Expression vector of mutant human galectin-9 [10-amino acid deletion with a single amino acid substitution at the linker region of G9Null], see Fig1 and TableS1 of Itoh, A. et al., Glycobiology, 23 (8): 920-925, 2013.
Comment Expression was confirmed by the depositor with SDS-PAGE.
Vector backbone pET-11a (Plasmid)
Selectable markers Amp^r
Gene/insert name Human LGALS9 cDNA
Depositor|Developer Nishi, Nozomu |
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External Database
Human LGALS9

          Reference sequence
            

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
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          RDB15282 pET-ssG9 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Itoh, A., Optimization of the inter-domain structure of galectin-9 for recombinant production. Glycobiology 23 (8): 920-925 (2013). PMID 23507964.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15282_B0J6p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBR_BamHI_R2
          Region: T7 pro, lac O, insert 5'
          Sequence file: RDB15282_B0J6a.seq check
          >D04748A1_B0J6_1_pBR_BamHI_R2_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCGTCCG GNNNNNNANG NNCGANNTCT
             61 CGNTCCCGCG AAATTAATAC GACTCACTAT AGGGGAATTG TGAGCGGATA ACAATTCCCC
            121 TCTAGAAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGCCTT CAGCGGTTCC
            181 CAGGCTCCCT ACCTGAGTCC AGCTGTCCCC TTTTCTGGGA CTATTCAAGG AGGTCTCCAG
            241 GACGGACTTC AGATCACTGT CAATGGGACC GTTCTCAGCT CCAGTGGAAC CAGGTTTGCT
            301 GTGAACTTTC AGACTGGCTT CAGTGGAAAT GACATTGCCT TCCACTTCAA CCCTCGGTTT
            361 GAAGATGGAG GGTACGTGGT GTGCAACACG AGGCAGAACG GAAGCTGGGG GCCCGAGGAG
            421 AGGAAGACAC ACATGCCTTT CCAGAAGGGG ATGCCCTTTG ACCTCTGCTT CCTGGTGCAG
            481 AGCTCAGATT TCAAGGTGAT GGTGAACGGT ATCCTCTTCG TGCAGTACTT CCACCGCGTG
            541 CCCTTCCACC GTGTGGACAC CATCTCCGTC AATGGCTCTG TGCAGCTGTC CTACATCAGC
            601 TTCCAGCACC CCCCCTATCC GATGCCTTTC ATCACCACCA TTCTGGGAGG GCTGTACCCA
            661 TCCAAGTCCA TCCTCCTGTC AGGCACTGTC CTGCCCAGTG CTCAGAGGTT CCACATCAAC
            721 CTGTGCTCTG GGAACCACAT CGCCTTCCAC CTGAACCCCC GTTTTGATGA GAATGCTGTG
            781 GTCCGCANCA CCCANATCGA CAACTCCNGG GGGGTCTGAN GANCGAANTC TGNCCCCNAA
            841 AAATGNNCTT CNTCCGTGGN CAGANCTTCT CANNNTGGAN CTTGTGTGNA NNCTCACTGC
            901 CTCNNGGNGG CCGTGNATGG NCAGCACCTG TTTGAATACT ACCATCNCNT GAGGANNCTG
          961 CCCACNNNTC NACANNNNNN NNNN
          //
          Primer: T7ter_R1
          Region: insert 3'
          Sequence file: RDB15282_B0J6b.seq check
          >D04748A1_B0J6_1_T7ter_R1_D02_04_ABI08.ab1
              1 NNNNNNNNNN NNNNCNNNTN NNTGNTNNGC GGNGGCAGCA GCCNACTCAG CTTCCTTTCG
             61 GGCTTTGTTA GCAGCCGGNT CCCTATGTCT GCACATGGGT CAGCTGGATG TCGCCCCCCA
            121 CTTCCAGTCT GTTGATGGTG GGCAGGTTCC TCAGGCGATG GTAGTATTCA AACAGGTGCT
            181 GACCATCCAC GGCCACCTTG AGGCAGTGAG CTTCACACAA GATCCACACT GAGAAGCTCT
            241 GGCCACGGAC GAAGGGCATT TTTCGGGGCA GACTTCGCTC CTCAGACCCC CAGGAGTTGT
            301 CGATCTGGGT GTTGCGGACC ACAGCATTCT CATCAAAACG GGGGTTCAGG TGGAAGGCGA
            361 TGTGGTTCCC AGAGCACAGG TTGATGTGGA ACCTCTGAGC ACTGGGCAGG ACAGTGCCTG
            421 ACAGGAGGAT GGACTTGGAT GGGTACAGCC CTCCCAGAAT GGTGGTGATG AAAGGCATCG
            481 GATAGGGGGG GTGCTGGAAG CTGATGTAGG ACAGCTGCAC AGAGCCATTG ACGGAGATGG
            541 TGTCCACACG GTGGAAGGGC ACGCGGTGGA AGTACTGCAC GAAGAGGATA CCGTTCACCA
            601 TCACCTTGAA ATCTGAGCTC TGCACCAGGA AGCAGAGGTC AAAGGGCATC CCCTTCTGGA
            661 AAGGCATGTG TGTCTTCCTC TCCTCGGGCC CCCAGCTTCC GTTCTGCCTC GTGTTGCACA
            721 CCACGTACCC TCCATCTTCA AACCGAGGGT TGAAGTGGAA NGCAATGTCA TTTCCACTGA
            781 AGCCAGTCTG AAAGTTCACA GCANNCCTGG TTCCACTGGA NCTGANAACG GTCCCATTGA
            841 CAGTGNTCTG AANTCCGTCC TGGANANCTC CTTNAATAGT CCCAGAAAAN GGGANAGCTG
            901 GNNTCNNNTA GGGNNCCNGG NAANNGCTGN NNNNNNTATN NATATCTCCT TCTTNAAGTN
          961 NAACNAANNN NNTTCNANNN GGGNANNNNN NNNNN
          //
          Primer: Tet-r-M_R
          Region: lacI
          Sequence file: RDB15282_B0J6c.seq check
          >D04748A1_B0J6_1_Tet-r-M_R_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNGN NCTGNNTGGG TTGANGGNTC TCAAGGGCAT CGGTCGAGAT
             61 CCCGGTGCCT AATGAGTGAG CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
            121 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
            181 GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG
            241 ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC
            301 CAGCAGGCGA AAATCCTGTT TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC
            361 GGTATCGTCG TATCCCACTA CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT
            421 GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT
            481 GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC
            541 CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG
            601 CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA
            661 TGCGACCAGA TGCTCCACGC CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT
            721 GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA ACATTAGTGC ANGCAGCTTC
            781 CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG
            841 CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCNANG NNGCTTCNTT CTANNNTCNA
            901 CNNCACCACG CNNGCACCCA NTTGNTCNNC GCNNNANTNN ANCGCCGNNN NNNTTNNCNN
            961 NNGCNCGNNN NNGNNCNNNN NNGGNNNNGG NNNNNNCNNN NNNNNNANNN NNNNNTNNNC
          1021 NNNCNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Itoh, A., Optimization of the inter-domain structure of galectin-9 for recombinant production. Glycobiology 23 (8): 920-925 (2013). PMID 23507964.
          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl