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pGEX-G2-1a

Bacteria expression vector of human galectin-2, GST fusion protein

Catalog number RDB04218
Resource name pGEX-G2-1a
Clone info. Expression vector of human galectin-2.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 2 cDNA
Depositor|Developer Nishi, Nozomu |
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External Database
Human lectin, galactoside-binding, soluble, 2

          Reference sequence
            

          Distribution information

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          RDB04218 pGEX-G2-1a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB04218.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17482_A9Hsp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pJB8-Forward
          Region: insert, thrombin site, GST 3'
          Sequence file: RDB17482_A9Hsa.seq check
          >04218_17482_A9Hs_2_JB8-Forward_A07_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT NGGTNNNGGC TGCGCCCCGA CNCCCGCCNA CACCCGCTGA
             61 CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC
            121 CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGATCGT
            181 CAGTCAGTCA CGATGCGGCC GCTCGAGTCG ACCCGGGAAT TCTTATTCTT TTAACTTGAA
            241 AGAGGACATG TTGAACCCGC CCCTTACGCT CAGGTAGCTC AGGTGGCTGT GACCCAGCCT
            301 GTTGGGAAAA GTCAGCTCGT GCCCATCTGG CAGCTTCACC TTGAATTTGT CACTCTCAAA
            361 GGTCACTGTG AACTTGACCT CTGACCCTGG GCTGAAGCAC AGGTGATCTT CCCGTTGTTC
            421 TTGCCCCCAG TTGCTGCCGT CCAATGAGTT GCAGACAATG GTGGATTCGC TGAAGCGAGG
            481 GTTGAAATGC AGGTTCAGCT TGTCTGTCCC CTGGCCCAGA TTAATTACAA AGCCATCAGT
            541 GCCATCGGCG ATGCTGCCTG TGATCTTCAG GGTTGACCCC GGCTTCATGT CCATGTTCTT
            601 AACCTCAAGT TCCCCCGTCA TGGATCCACG CGGAACCAGA TCCGATTTTG GAGGATGGTC
            661 GCCACCACCA AACGTGGCTT GCCAGCCCTG CAAAGGCCAT GCTATATACT TGCTGGATTT
            721 CAAGTACTTA TCAATTTGTG GGATAGCTTC NATACGTTTT TTAAAACAAA CTAATTTTGG
            781 GAACGCATCC NGGCACATTG GGTCCATGTA TAAAACAACA TCAAGAGCGT CATACAACAT
            841 GAAGTCAGGA TGGGTTACAT GATCACCATT TAAATATGTT NTATGACATN AACNATCTTC
            901 NAANATTTTC AGCATTTCAN GTAGCTTGCT NNGAAAATCA ANTTTNAGAG NTTCAAAGTC
          961 TTTA
          //
          Primer: pUC-M13_-100
          Region: lacZ alpha, lacO, lacI gene
          Sequence file: RDB17482_A9Hsb.seq check
          >04218_17482_A9Hs_2_pUC-M13_-100_B07_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG GGNNNGCCNG GGTTTTCCCN GTCNCGACGT TGTAAAACGA
             61 CGGCCAGTGA ATCCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
            121 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
            181 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
            241 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
            301 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC
            361 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC
            421 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC
            481 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC
            541 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT
            601 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG
            661 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC
            721 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC
            781 CACGCCCAGT CGCGTANCGN CTTCATGGGA GAAAATAATA CTGNTGATGG GTGTCTGGNC
            841 AGAGACATCA AGAAATANCG CCNGANCATT AGTGCAGGNA GCTNCCACAG CAATGGCATC
          901 CTGGTCNTCC NGCGGNNANT TAATGATCAG CCNACNNANG CGTTGCNCN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl