Please review the QC test results indicated by icon below as well as clone information before placing your order.

pELN-2

Bacterial expression vector of porcine elastin (ELN) short form.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15986_A8Fip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: GST-M_F () Region: GST3',thrombin site,insert5' Sequence file: RDB15986_A8Fia.seq
>D05595A4-1_A8Fi_GST-M_F_E02_14_ABI24.ab1     1 TTAGACCTTT GAACTCTCAA GTTGATTTTC TTAGCAAGCT ACCTGAAATG CTGAAAATGT    61 TCGAAGATCG TTTATGTCAT AAAACATATT TAAATGGTGA TCATGTAACC CATCCTGACT   121 TCATGTTGTA TGACGCTCTT GATGTTGTTT TATACATGGA CCCAATGTGC CTGGATGCGT   181 TCCCAAAATT AGTTTGTTTT AAAAAACGTA TTGAAGCTAT CCCACAAATT GATAAGTACT   241 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG   301 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CGGAGGGGTC CCAGGGGCTG   361 TTCCTGGGGG AGTTCCCGGA GGCGTCTTTT TCCCAGGGGC TGGTCTCGGA GGCCTGGGAG   421 GAGGAGCACT GGGCCCTGGA GGCAAGCCAC CCAAGCCAGG TGTCGGAGGG CTCGCAGGCG   481 CTGGCCTTGG GGCAGGGCTC GGGGCCTTTC CTGCAGGCGC CTTCCCAGGG GCTCTGGTGC   541 CCGGCGGCGT GGCTGACGCG GCCGCAGCCT ATAAAGCTGC TGCCAAGGCT GGTGCCGGGC   601 TTGGCGGCGT TGGTGGTGTC GGCGGCTTAG GAGTGTCTAC AGGTGCAGTG GTACCTCAAC   661 TCGGAGCAGG AGTTGGAGCT GGCGCGAAGC CAGGGAAAGT ACCAGGTGTG GGGCTCCCAG   721 GTGTCTACCC TGGTGGAGTG CTCCCGGGCA CAGGAGCTCG GTTCCCAGGT GTAGGGGTGC   781 TCCCTGGCGT TCCCACTGGA ACAGGAGTCA AGGCCAAGGC CCCAGGTGGA GGTGGAGCTT   841 TTGCTGGAAT CCCAGGAGTT GGACCCTTTG GAGGTCAGCA GCCTGGAGTC CCCCCTGGGG   901 TACCCCATCA AGGCACCCAA GCTGCCAGGG GGTTGGTACA CAAGCCCGCA GCAGCAGCAG   961 CAGCAGCTAA AGCAGCAGCT AAATATGGTG CCCCCGGGAG CCCGGCGTTT CTCCCTGGTC  1021 GTCCGGCGGT CGGAGTGGTC GGCGTTCTTG GCCGGGCGCG CGGATTTCCT GTCATCCGGA 1081 GGGCATTGCA GGGGGACCGG GAGCCTTCCC CA //
Primer: pJB8-Forward () Region: insert3' Sequence file: RDB15986_A8Fib.seq
>D05595A4-1_A8Fi_pJB8-Forward_F02_17_ABI24.ab1     1 TACAATTGGC CTTCGGGACT GGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG    61 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT   121 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGATCG   181 TCAGTCAGTC ACGATGCGGC CGCTCGAGTC GACCCGGGAA TTCTCACTTT CTCTTCCGGC   241 CACAGGATTT CCCCAGGCAG GCCCCACCTT GGTATCCCAG GGCTCCCAGG GCCCCTCCGA   301 AGGGCTTGGG AGGTTTGCCA CCAATTCCCA GGCCCCCAGC GCCGCCACCT GGGAAAATAG   361 GAGACAGTCC AAAGCCAGGC CTTGGTGCGA CTCCTCCAAG TGGGAATGGC CTGGTGACTC   421 CTAGGACACC TCCGAGGCCA GCGGCACCAT ATTTGGCTGC TTTAGCAGCG GCAGCCGGGG   481 ACACACCTCC AAAGGCTCCA GCCCCTGGAA CAGCTCCAAG GCCCCCAACT CCCAGTCCTC   541 CGACTCCCCC GACGCCAAAC TGGGCAGCTT TGGCAGCGGC TTTGGCGGCA GCAGGTCCGA   601 CTCCTGCCAC ACCACCTGGG ATACCAGCTC CGCCAAGATC TCCAACCCCT CCAAGGGCCC   661 CGGCCGCCCC ATATTTGGCT GCTTTAGCTG CGGCCAGGGG TCCAGGTACT GCACCTGCCC   721 CAAAGCCAGG AACACCAGCA CCAACTCCAA ATCCAGGAAC GCCGGCACCA ACTCCAAATC   781 CGGGAACGCC GGCAGGAAGC CCAGCAGCAG CCTGGAACTG GGCTTTGGCG GCTGCCTTAG   841 CAGCAGATTT GGGCTGCAGC CGGGGCCCCT GCTCCTATAA CACCGCCAGG GCCAATGCCA   901 ATGCCGGGGA GCCACACCGA CGCCGGGGAG CCACACCGAC GCCGGGAGCC ACACCGGACG   961 CCGGGAGCCC ACACCGACGC CCGGGGAGCC ACAACCAAAT GCCCAGGGCC CTAAGCCGGA  1021 AACTGGGCGG GCTTTGGGCC GGCTGCTTTT GGCGGGCTGC AGCCTGCCTG GGCCTTCCTA 1081 CCCCCTGGGC ACTGCTTCAA GCAACGGGCA CACCCTTGGA TTACTTGCTT CCATC //
Primer: ColE1ori_F () Region: ori,lacI pro,lacI5' Sequence file: RDB15986_A8Fic.seq
>D05595A4-1_A8Fi_ColE1ori_F_G02_20_ABI24.ab1     1 CCCCCTTCCT GGAACCTTGG GGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGGCC    61 TATGGAAAAA CGCCATGTCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT   121 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT   181 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA   241 GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA   301 CCGCATAAAT TCCGACACCA TCGAATGGCG CAAAACCTTT CGCGGTATGG CATGATAGCG   361 CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT ACGATGTCGC   421 AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG CCAGCCACGT   481 TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT ACATTCCCAA   541 CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG CCACCTCCAG   601 TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG CCGATCAACT   661 GGGTGCCAGG AGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGGC CTGTAAAGCG   721 GCGGTGCAAC AATCTTCTCG CGCAACGCGT CAGTGGGGCT GATCATTAAC TATCCGCTGG   781 ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTTATTTCTT   841 GATGTCTCTG ACCAGACACC CAATCAAACA GGTATTATTT GTCTCCCATG AAAGAACGGG   901 TACGCGACTG GGGCGTGGGA GTCATTCTGG GTCGGCAATT GGGGTCACCA GCAAATCGCG   961 GCTGTTAGCG GGGCCCATTT AAAGTTCTGT CCTCGGCGCG TCCTGCGTTC TGGGCTGGAC 1021 TGGGCAATAA ATTATCCTCA ACTTCGC //
Primer: pGEX-ptac_UP-F () Region: tac pro,GST Sequence file: RDB15986_A8Fid.seq
>D05595A4-1_A8Fi_pGEX-ptac_UP-F_H02_23_ABI24.ab1     1 TGAGTGGGAA ATAATAACGG GTTCTGGCAA TATTCTGAAA TGAGCTGTTG ACAATTAATC    61 ATCGGCTCGT ATAATGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGTAT   121 TCATGTCCCC TATACTAGGT TATTGGAAAA TTAAGGGCCT TGTGCAACCC ACTCGACTTC   181 TTTTGGAATA TCTTGAAGAA AAATATGAAG AGCATTTGTA TGAGCGCGAT GAAGGTGATA   241 AATGGCGAAA CAAAAAGTTT GAATTGGGTT TGGAGTTTCC CAATCTTCCT TATTATATTG   301 ATGGTGATGT TAAATTAACA CAGTCTATGG CCATCATACG TTATATAGCT GACAAGCACA   361 ACATGTTGGG TGGTTGTCCA AAAGAGCGTG CAGAGATTTC AATGCTTGAA GGAGCGGTTT   421 TGGATATTAG ATACGGTGTT TCGAGAATTG CATATAGTAA AGACTTTGAA ACTCTCAAAG   481 TTGATTTTCT TAGCAAGCTA CCTGAAATGC TGAAAATGTT CGAAGATCGT TTATGTCATA   541 AAACATATTT AAATGGTGAT CATGTAACCC ATCCTGACTT CATGTTGTAT GACGCTCTTG   601 ATGTTGTTTT ATACATGGAC CCAATGTGCC TGGATGCGTT CCCAAAATTA GTTTGTTTTA   661 AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTACTT GAAATCCAGC AAGTATATAG   721 CATGGCCTTT GCAGGGCTGG CAAGCCACGT TTGGTGGTGG CGACCATCCT CCAAAATCGG   781 ATCTGGTTCC GCGTGGATCC GGAGGGGTCC CAGGGGCTGT TCCTGGGGGA GTTCCCGGAG   841 GCGTCTTTTT CCCAGGGGCT GGTCTCGGAG GCCTGGGAGG AGGAGCACTG GGCCCTGGAG   901 GCAAGCCACC CAAGCCAGGT GTCGGAGGGC TCGCAGGCGC TGGCCTTGGG GGCAAGGGCT   961 CGGGGGCCTT TCCTGCAGGC GCCCTTTCCC AAGGGGGCTC TGGGTGCCCG GGCGGGCGTG  1021 GGCTGAACGC GGGCCCGCAG CCCTATAAGC TGCTGCCCAA AGGCTGGGTG CCGGGCTTTG  1081 GACGGCGTTT GGGTGTGTCC GGCGGCCTTA AGGAAGTGTC CTAACAGGGT TGCCAAGTTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.17

GNP_filter3_RDBDEP_html_230702.pl