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pELN-2

Bacterial expression vector of porcine elastin (ELN) short form.

Catalog number RDB15986
Resource name pELN-2
Alternative name Porcine elastin 2
Clone info. Bacterial expression vector of porcine elastin (ELN), short form in pGEX-4T-2. Please note that this clone contains no signal peptide sequence.
Vector backbone pGEX-4T-2 (plasmid)
Size of vector backbone 4.9 kb
Selectable markers Amp^r
Gene/insert name sus scrofa (porcine) ELN cDNA
Reference of nucleotide sequence LC005980 (DDBJ accession number)
Depositor Nishi, Nozomu |
 
Other clones in our bank sus scrofa (porcine) ELN (KEGG orthology K14211) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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RDB15986 pELN-2 DNA solution

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References and tips

Electronic file

Original reference

original Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15986_A8Fip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: GST3',thrombin site,insert5' | Primer: GST-M_F | Sequence: RDB15986_A8Fia.seq
>D05595A4-1_A8Fi_GST-M_F_E02_14_ABI24.ab1
    1 TTAGACCTTT GAACTCTCAA GTTGATTTTC TTAGCAAGCT ACCTGAAATG CTGAAAATGT
   61 TCGAAGATCG TTTATGTCAT AAAACATATT TAAATGGTGA TCATGTAACC CATCCTGACT
  121 TCATGTTGTA TGACGCTCTT GATGTTGTTT TATACATGGA CCCAATGTGC CTGGATGCGT
  181 TCCCAAAATT AGTTTGTTTT AAAAAACGTA TTGAAGCTAT CCCACAAATT GATAAGTACT
  241 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
  301 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CGGAGGGGTC CCAGGGGCTG
  361 TTCCTGGGGG AGTTCCCGGA GGCGTCTTTT TCCCAGGGGC TGGTCTCGGA GGCCTGGGAG
  421 GAGGAGCACT GGGCCCTGGA GGCAAGCCAC CCAAGCCAGG TGTCGGAGGG CTCGCAGGCG
  481 CTGGCCTTGG GGCAGGGCTC GGGGCCTTTC CTGCAGGCGC CTTCCCAGGG GCTCTGGTGC
  541 CCGGCGGCGT GGCTGACGCG GCCGCAGCCT ATAAAGCTGC TGCCAAGGCT GGTGCCGGGC
  601 TTGGCGGCGT TGGTGGTGTC GGCGGCTTAG GAGTGTCTAC AGGTGCAGTG GTACCTCAAC
  661 TCGGAGCAGG AGTTGGAGCT GGCGCGAAGC CAGGGAAAGT ACCAGGTGTG GGGCTCCCAG
  721 GTGTCTACCC TGGTGGAGTG CTCCCGGGCA CAGGAGCTCG GTTCCCAGGT GTAGGGGTGC
  781 TCCCTGGCGT TCCCACTGGA ACAGGAGTCA AGGCCAAGGC CCCAGGTGGA GGTGGAGCTT
  841 TTGCTGGAAT CCCAGGAGTT GGACCCTTTG GAGGTCAGCA GCCTGGAGTC CCCCCTGGGG
  901 TACCCCATCA AGGCACCCAA GCTGCCAGGG GGTTGGTACA CAAGCCCGCA GCAGCAGCAG
  961 CAGCAGCTAA AGCAGCAGCT AAATATGGTG CCCCCGGGAG CCCGGCGTTT CTCCCTGGTC
 1021 GTCCGGCGGT CGGAGTGGTC GGCGTTCTTG GCCGGGCGCG CGGATTTCCT GTCATCCGGA
1081 GGGCATTGCA GGGGGACCGG GAGCCTTCCC CA
//
Region: insert3' | Primer: pJB8-Forward | Sequence: RDB15986_A8Fib.seq
>D05595A4-1_A8Fi_pJB8-Forward_F02_17_ABI24.ab1
    1 TACAATTGGC CTTCGGGACT GGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG
   61 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT
  121 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGATCG
  181 TCAGTCAGTC ACGATGCGGC CGCTCGAGTC GACCCGGGAA TTCTCACTTT CTCTTCCGGC
  241 CACAGGATTT CCCCAGGCAG GCCCCACCTT GGTATCCCAG GGCTCCCAGG GCCCCTCCGA
  301 AGGGCTTGGG AGGTTTGCCA CCAATTCCCA GGCCCCCAGC GCCGCCACCT GGGAAAATAG
  361 GAGACAGTCC AAAGCCAGGC CTTGGTGCGA CTCCTCCAAG TGGGAATGGC CTGGTGACTC
  421 CTAGGACACC TCCGAGGCCA GCGGCACCAT ATTTGGCTGC TTTAGCAGCG GCAGCCGGGG
  481 ACACACCTCC AAAGGCTCCA GCCCCTGGAA CAGCTCCAAG GCCCCCAACT CCCAGTCCTC
  541 CGACTCCCCC GACGCCAAAC TGGGCAGCTT TGGCAGCGGC TTTGGCGGCA GCAGGTCCGA
  601 CTCCTGCCAC ACCACCTGGG ATACCAGCTC CGCCAAGATC TCCAACCCCT CCAAGGGCCC
  661 CGGCCGCCCC ATATTTGGCT GCTTTAGCTG CGGCCAGGGG TCCAGGTACT GCACCTGCCC
  721 CAAAGCCAGG AACACCAGCA CCAACTCCAA ATCCAGGAAC GCCGGCACCA ACTCCAAATC
  781 CGGGAACGCC GGCAGGAAGC CCAGCAGCAG CCTGGAACTG GGCTTTGGCG GCTGCCTTAG
  841 CAGCAGATTT GGGCTGCAGC CGGGGCCCCT GCTCCTATAA CACCGCCAGG GCCAATGCCA
  901 ATGCCGGGGA GCCACACCGA CGCCGGGGAG CCACACCGAC GCCGGGAGCC ACACCGGACG
  961 CCGGGAGCCC ACACCGACGC CCGGGGAGCC ACAACCAAAT GCCCAGGGCC CTAAGCCGGA
 1021 AACTGGGCGG GCTTTGGGCC GGCTGCTTTT GGCGGGCTGC AGCCTGCCTG GGCCTTCCTA
1081 CCCCCTGGGC ACTGCTTCAA GCAACGGGCA CACCCTTGGA TTACTTGCTT CCATC
//
Region: ori,lacI pro,lacI5' | Primer: ColE1ori_F | Sequence: RDB15986_A8Fic.seq
>D05595A4-1_A8Fi_ColE1ori_F_G02_20_ABI24.ab1
    1 CCCCCTTCCT GGAACCTTGG GGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGGCC
   61 TATGGAAAAA CGCCATGTCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
  121 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT
  181 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA
  241 GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA
  301 CCGCATAAAT TCCGACACCA TCGAATGGCG CAAAACCTTT CGCGGTATGG CATGATAGCG
  361 CCCGGAAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA GTAACGTTAT ACGATGTCGC
  421 AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG GTGAACCAGG CCAGCCACGT
  481 TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG GAGCTGAATT ACATTCCCAA
  541 CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG ATTGGCGTTG CCACCTCCAG
  601 TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT AAATCTCGCG CCGATCAACT
  661 GGGTGCCAGG AGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGGC CTGTAAAGCG
  721 GCGGTGCAAC AATCTTCTCG CGCAACGCGT CAGTGGGGCT GATCATTAAC TATCCGCTGG
  781 ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG TTTATTTCTT
  841 GATGTCTCTG ACCAGACACC CAATCAAACA GGTATTATTT GTCTCCCATG AAAGAACGGG
  901 TACGCGACTG GGGCGTGGGA GTCATTCTGG GTCGGCAATT GGGGTCACCA GCAAATCGCG
  961 GCTGTTAGCG GGGCCCATTT AAAGTTCTGT CCTCGGCGCG TCCTGCGTTC TGGGCTGGAC
1021 TGGGCAATAA ATTATCCTCA ACTTCGC
//
Region: tac pro,GST | Primer: pGEX-ptac_UP-F | Sequence: RDB15986_A8Fid.seq
>D05595A4-1_A8Fi_pGEX-ptac_UP-F_H02_23_ABI24.ab1
    1 TGAGTGGGAA ATAATAACGG GTTCTGGCAA TATTCTGAAA TGAGCTGTTG ACAATTAATC
   61 ATCGGCTCGT ATAATGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGTAT
  121 TCATGTCCCC TATACTAGGT TATTGGAAAA TTAAGGGCCT TGTGCAACCC ACTCGACTTC
  181 TTTTGGAATA TCTTGAAGAA AAATATGAAG AGCATTTGTA TGAGCGCGAT GAAGGTGATA
  241 AATGGCGAAA CAAAAAGTTT GAATTGGGTT TGGAGTTTCC CAATCTTCCT TATTATATTG
  301 ATGGTGATGT TAAATTAACA CAGTCTATGG CCATCATACG TTATATAGCT GACAAGCACA
  361 ACATGTTGGG TGGTTGTCCA AAAGAGCGTG CAGAGATTTC AATGCTTGAA GGAGCGGTTT
  421 TGGATATTAG ATACGGTGTT TCGAGAATTG CATATAGTAA AGACTTTGAA ACTCTCAAAG
  481 TTGATTTTCT TAGCAAGCTA CCTGAAATGC TGAAAATGTT CGAAGATCGT TTATGTCATA
  541 AAACATATTT AAATGGTGAT CATGTAACCC ATCCTGACTT CATGTTGTAT GACGCTCTTG
  601 ATGTTGTTTT ATACATGGAC CCAATGTGCC TGGATGCGTT CCCAAAATTA GTTTGTTTTA
  661 AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTACTT GAAATCCAGC AAGTATATAG
  721 CATGGCCTTT GCAGGGCTGG CAAGCCACGT TTGGTGGTGG CGACCATCCT CCAAAATCGG
  781 ATCTGGTTCC GCGTGGATCC GGAGGGGTCC CAGGGGCTGT TCCTGGGGGA GTTCCCGGAG
  841 GCGTCTTTTT CCCAGGGGCT GGTCTCGGAG GCCTGGGAGG AGGAGCACTG GGCCCTGGAG
  901 GCAAGCCACC CAAGCCAGGT GTCGGAGGGC TCGCAGGCGC TGGCCTTGGG GGCAAGGGCT
  961 CGGGGGCCTT TCCTGCAGGC GCCCTTTCCC AAGGGGGCTC TGGGTGCCCG GGCGGGCGTG
 1021 GGCTGAACGC GGGCCCGCAG CCCTATAAGC TGCTGCCCAA AGGCTGGGTG CCGGGCTTTG
 1081 GACGGCGTTT GGGTGTGTCC GGCGGCCTTA AGGAAGTGTC CTAACAGGGT TGCCAAGTTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189.

2019.09.06

GNP_filter3_RDBDEP_html_190816.pl