Resource data sheet
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pELN-1

Bacterial expression vector of porcine elastin (ELN) long form.

Catalog number RDB15985
Resource name pELN-1
Alternative name Porcine elastin 1
Clone info. Bacterial expression vector of porcine elastin (ELN), long form in pGEX-4T-2. Please note that this clone contains no signal peptide sequence.
Vector backbone pGEX-4T-2 (plasmid)
Size of vector backbone 4.9 kb
Selectable markers Amp^r
Gene/insert name sus scrofa (porcine) ELN cDNA
Reference of nucleotide sequence LC005979 (DDBJ accession number)
Depositor Nishi, Nozomu |
 
Other clones in our bank sus scrofa (porcine) ELN (KEGG orthology K14211) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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RDB15985 pELN-1 DNA solution

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References and tips

Electronic file

Original reference

original Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15985_A8Fip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: GST3',thrombin site,insert5' | Primer: GST-M_F | Sequence: RDB15985_A8Fia.seq
>D05595A3-2_A8Fi_GST-M_F_A02_02_ABI24.ab1
    1 TATAGACTTT GAACTCTCAA GTTGATTTTC TTAGCAAGCT ACCTGAAATG CTGAAAATGT
   61 TCGAAGATCG TTTATGTCAT AAAACATATT TAAATGGTGA TCATGTAACC CATCCTGACT
  121 TCATGTTGTA TGACGCTCTT GATGTTGTTT TATACATGGA CCCAATGTGC CTGGATGCGT
  181 TCCCAAAATT AGTTTGTTTT AAAAAACGTA TTGAAGCTAT CCCACAAATT GATAAGTACT
  241 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
  301 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CGGAGGGGTC CCAGGGGCTG
  361 TTCCTGGGGG AGTTCCCGGA GGCGTCTTTT TCCCAGGGGC TGGTCTCGGA GGCCTGGGAG
  421 GAGGAGCACT GGGCCCTGGA GGCAAGCCAC CCAAGCCAGG TGTCGGAGGG CTCGCAGGCG
  481 CTGGCCTTGG GGCAGGGCTC GGGGCCTTTC CTGCAGGCGC CTTCCCAGGG GCTCTGGTGC
  541 CCGGCGGCGT GGCTGACGCG GCCGCAGCCT ATAAAGCTGC TGCCAAGGCT GGTGCCGGGC
  601 TTGGCGGCGT TGGTGGTGTC GGCGGCTTAG GAGTGTCTAC AGGTGCAGTG GTACCTCAAC
  661 TCGGAGCAGG AGTTGGAGCT GGCGCGAAGC CAGGGAAAGT ACCAGGTGTG GGGCTCCCAG
  721 GTGTCTACCC TGGTGGAGTG CTCCCGGGCA CAGGAGCTCG GTTCCCAGGT GTAGGGGTGC
  781 TCCCTGGCGT TCCCACTGGA ACAGGAGTCA AGGCCAAGGC CCCAGGTGGA GGTGGAGCTT
  841 TTGCTGGAAT CCCAGGAGTT GGACCCTTTG GAGGTCAGCA GCCTGGAGTC CCCCCTGGGG
  901 TACCCCATCA AGGCACCCAA GCTGCCAGGT GGCTACGGAC TGCCCTACAG CACTGGGAAA
  961 CTGCCCTACG GCTTTGGGCC CTGGAGGAGT GGCTGGCGCC GCGGGCAAAG GCTGGGTACC
 1021 CAACGGGGAC GGGGGTTTGG GTACACAAGT CTGCAGCAGC AGCAGCAGCC AGCCTAAAGG
1081 CCAGCCAGCT AAAAATATGG GTGTCCA
//
Region: insert3' | Primer: pJB8-Forward | Sequence: RDB15985_A8Fib.seq
>D05595A3-2_A8Fi_pJB8-Forward_B02_05_ABI24.ab1
    1 GACCATATCG CATGGTGAAC TGGGGTCATG GCTGCGCCCC GACAACCCGC CAAACACCCG
   61 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG
  121 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGA
  181 TCGTCAGTCA GTCACGATGC GGCCGCTCGA GTCGACCCGG GAATTCTCAC TTTCTCTTCC
  241 GGCCACAGGA TTTCCCCAGG CAGGCCCCAC CTTGGTATCC CAGGGCTCCC AGGGCCCCTC
  301 CGAAGGGCTT GGGAGGTTTG CCACCAATTC CCAGGCCCCC AGCGCCGCCA CCTGGGAAAA
  361 TAGGAGACAG TCCAAAGCCA GGCCTTGGTG CGACTCCTCC AAGTGGGAAT GGCCTGGTGA
  421 CTCCTAGGAC ACCTCCGAGG CCAGCGGCAC CATATTTGGC TGCTTTAGCA GCGGCAGCCG
  481 GGGACACACC TCCAAAGGCT CCAGCCCCTG GAACAGCTCC AAGGCCCCCA ACTCCCAGTC
  541 CTCCGACTCC CCCGACGCCA AACTGGGCAG CTTTGGCAGC GGCTTTGGCG GCAGCAGGTC
  601 CGACTCCTGC CACACCACCT GGGATACCAG CTCCGCCAAG ATCTCCAACC CCTCCAAGGG
  661 CCCCGGCCGC CCCATATTTG GCTGCTTTAG CTGCGGCCAG GGGTCCAGGT ACTGCACCTG
  721 CCCCAAAGCC AGGAACACCA GCACCAACTC CAAATCCAGG AACGCCGGCA CCAACTCCAA
  781 ATCCGGGAAC GCCGGCAGGA AGCCCAGCAG CAGCCTGGAA CTGGGGCTTT GGCGGCTGCC
  841 TTAGCAGCAG ATTTGGCTGC AGCCGGGGCC CCCTGCTCCT ATAACACCGC CAGGGCCAAT
  901 GCCAATGCCG GGAGCCACAC CGACGCCGGG AGCCACACCG ACGCCGGGGA GCCACACCGA
  961 CGCCGGGAGC CACACCGACG CCGGGAGCCA CACCAATGCC AGGGCCCTAA GCCGAACTGG
 1021 GGCGGCTTTG GGCGGCTGCC TTTTGGCGGC CTGCAGCTGG CTGGGCTCCC TACCCCTGGC
1081 ACTGCTCCAG CCACCCGGGG CCAACCACCC C
//
Region: ori,lacI pro,lacI5' | Primer: ColE1ori_F | Sequence: RDB15985_A8Fic.seq
>D05595A3-2_A8Fi_ColE1ori_F_C02_08_ABI24.ab1
    1 GGTAGGTGCA TGTGGGGTTC GCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
   61 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
  121 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
  181 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
  241 TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC
  301 GGTATTTCAC ACCGCATAAA TTCCGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG
  361 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA
  421 TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG
  481 GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT
  541 TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT
  601 GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC
  661 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC
  721 TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT
  781 CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA
  841 TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTTCTCCC ATGAAGACGG
  901 TACGCGACTG GGCGTGGAGC ATCTGGGTCG CATTGGGGTC ACCAGCAAAT CGCGGCTGTT
  961 AGCGGGGCCC ATTTAAGTTC TGTCTCGGCC GCGTCTGCGT CTGGGCTGGC TGGCAATAAA
 1021 TATCTCACTC GGCAATCAAA TTCAGGCCGG ATTAGCGGGA ACGGAAGGCG AACTGGAGTG
1081 GCATGATCCG GTTTCAAACA AAAACCCATG TGCCAAAAA
//
Region: tac pro,GST | Primer: pGEX-ptac_UP-F | Sequence: RDB15985_A8Fid.seq
>D05595A3-2_A8Fi_pGEX-ptac_UP-F_D02_11_ABI24.ab1
    1 ATGGGCGGGC TATACGGTTC TGGCAATATT CTGAAATGAG CTGTTGACAA TTAATCATCG
   61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT
  121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT
  181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG
  241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG
  301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT
  361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA
  421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA
  481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC
  541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT
  601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA
  661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG
  721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT
  781 GGTTCCGCGT GGATCCGGAG GGGTCCCAGG GGCTGTTCCT GGGGGAGTTC CCGGAGGCGT
  841 CTTTTTCCCA GGGGCTGGTC TCGGAGGCCT GGGAGGAGGA GCACTGGGCC CTGGAGGCAA
  901 GCCACCCAAG CCAGGTGTCG GAGGGCTCGC AGGCGCTGGG CCTTGGGGGC AGGGCTCGGG
  961 GCCTTTCCTG CAGGCGCCCT TCCCAGGGGG CTCTGGGTGC CCGGGCGGGC GTGGCTGACG
 1021 CGGGCCGCAG CCTATAAAGC TGCTGCCAAC GCCTGCTGCG GGCCTGCCGG CCGATGGGTT
1081 GCTGTCGGCG GCTTTAGGAG TGGTTCTTAA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189.

2019.09.06

GNP_filter3_RDBDEP_html_190816.pl