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pELN-1

Bacterial expression vector of porcine elastin (ELN) long form.

Catalog number RDB15985
Resource name pELN-1
Alternative name Porcine elastin 1
Clone info. Bacterial expression vector of porcine elastin (ELN), long form in pGEX-4T-2. Please note that this clone contains no signal peptide sequence.
Vector backbone pGEX-4T-2 (plasmid)
Size of vector backbone 4.9 kb
Selectable markers Amp^r
Gene/insert name sus scrofa (porcine) ELN cDNA
Reference of insert sequence LC005979 (DDBJ accession number)
Depositor|Developer Nishi, Nozomu |
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External Database
sus scrofa (porcine) ELN

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017)。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

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          RDB15985 pELN-1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pELN-1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15985).

          Reference section:

          Itoh, A., Nonaka, Y., Ogawa, T., Nakamura, T., Nishi, N., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15985_A8Fip1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: GST-M_F ()
          Region: GST3',thrombin site,insert5'
          Sequence file: RDB15985_A8Fia.seq check
          >D05595A3-2_A8Fi_GST-M_F_A02_02_ABI24.ab1
              1 TATAGACTTT GAACTCTCAA GTTGATTTTC TTAGCAAGCT ACCTGAAATG CTGAAAATGT
             61 TCGAAGATCG TTTATGTCAT AAAACATATT TAAATGGTGA TCATGTAACC CATCCTGACT
            121 TCATGTTGTA TGACGCTCTT GATGTTGTTT TATACATGGA CCCAATGTGC CTGGATGCGT
            181 TCCCAAAATT AGTTTGTTTT AAAAAACGTA TTGAAGCTAT CCCACAAATT GATAAGTACT
            241 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
            301 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CGGAGGGGTC CCAGGGGCTG
            361 TTCCTGGGGG AGTTCCCGGA GGCGTCTTTT TCCCAGGGGC TGGTCTCGGA GGCCTGGGAG
            421 GAGGAGCACT GGGCCCTGGA GGCAAGCCAC CCAAGCCAGG TGTCGGAGGG CTCGCAGGCG
            481 CTGGCCTTGG GGCAGGGCTC GGGGCCTTTC CTGCAGGCGC CTTCCCAGGG GCTCTGGTGC
            541 CCGGCGGCGT GGCTGACGCG GCCGCAGCCT ATAAAGCTGC TGCCAAGGCT GGTGCCGGGC
            601 TTGGCGGCGT TGGTGGTGTC GGCGGCTTAG GAGTGTCTAC AGGTGCAGTG GTACCTCAAC
            661 TCGGAGCAGG AGTTGGAGCT GGCGCGAAGC CAGGGAAAGT ACCAGGTGTG GGGCTCCCAG
            721 GTGTCTACCC TGGTGGAGTG CTCCCGGGCA CAGGAGCTCG GTTCCCAGGT GTAGGGGTGC
            781 TCCCTGGCGT TCCCACTGGA ACAGGAGTCA AGGCCAAGGC CCCAGGTGGA GGTGGAGCTT
            841 TTGCTGGAAT CCCAGGAGTT GGACCCTTTG GAGGTCAGCA GCCTGGAGTC CCCCCTGGGG
            901 TACCCCATCA AGGCACCCAA GCTGCCAGGT GGCTACGGAC TGCCCTACAG CACTGGGAAA
            961 CTGCCCTACG GCTTTGGGCC CTGGAGGAGT GGCTGGCGCC GCGGGCAAAG GCTGGGTACC
           1021 CAACGGGGAC GGGGGTTTGG GTACACAAGT CTGCAGCAGC AGCAGCAGCC AGCCTAAAGG
          1081 CCAGCCAGCT AAAAATATGG GTGTCCA
          //
          Primer: pJB8-Forward ()
          Region: insert3'
          Sequence file: RDB15985_A8Fib.seq check
          >D05595A3-2_A8Fi_pJB8-Forward_B02_05_ABI24.ab1
              1 GACCATATCG CATGGTGAAC TGGGGTCATG GCTGCGCCCC GACAACCCGC CAAACACCCG
             61 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG
            121 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGA
            181 TCGTCAGTCA GTCACGATGC GGCCGCTCGA GTCGACCCGG GAATTCTCAC TTTCTCTTCC
            241 GGCCACAGGA TTTCCCCAGG CAGGCCCCAC CTTGGTATCC CAGGGCTCCC AGGGCCCCTC
            301 CGAAGGGCTT GGGAGGTTTG CCACCAATTC CCAGGCCCCC AGCGCCGCCA CCTGGGAAAA
            361 TAGGAGACAG TCCAAAGCCA GGCCTTGGTG CGACTCCTCC AAGTGGGAAT GGCCTGGTGA
            421 CTCCTAGGAC ACCTCCGAGG CCAGCGGCAC CATATTTGGC TGCTTTAGCA GCGGCAGCCG
            481 GGGACACACC TCCAAAGGCT CCAGCCCCTG GAACAGCTCC AAGGCCCCCA ACTCCCAGTC
            541 CTCCGACTCC CCCGACGCCA AACTGGGCAG CTTTGGCAGC GGCTTTGGCG GCAGCAGGTC
            601 CGACTCCTGC CACACCACCT GGGATACCAG CTCCGCCAAG ATCTCCAACC CCTCCAAGGG
            661 CCCCGGCCGC CCCATATTTG GCTGCTTTAG CTGCGGCCAG GGGTCCAGGT ACTGCACCTG
            721 CCCCAAAGCC AGGAACACCA GCACCAACTC CAAATCCAGG AACGCCGGCA CCAACTCCAA
            781 ATCCGGGAAC GCCGGCAGGA AGCCCAGCAG CAGCCTGGAA CTGGGGCTTT GGCGGCTGCC
            841 TTAGCAGCAG ATTTGGCTGC AGCCGGGGCC CCCTGCTCCT ATAACACCGC CAGGGCCAAT
            901 GCCAATGCCG GGAGCCACAC CGACGCCGGG AGCCACACCG ACGCCGGGGA GCCACACCGA
            961 CGCCGGGAGC CACACCGACG CCGGGAGCCA CACCAATGCC AGGGCCCTAA GCCGAACTGG
           1021 GGCGGCTTTG GGCGGCTGCC TTTTGGCGGC CTGCAGCTGG CTGGGCTCCC TACCCCTGGC
          1081 ACTGCTCCAG CCACCCGGGG CCAACCACCC C
          //
          Primer: ColE1ori_F ()
          Region: ori,lacI pro,lacI5'
          Sequence file: RDB15985_A8Fic.seq check
          >D05595A3-2_A8Fi_ColE1ori_F_C02_08_ABI24.ab1
              1 GGTAGGTGCA TGTGGGGTTC GCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
             61 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
            121 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
            181 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
            241 TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC
            301 GGTATTTCAC ACCGCATAAA TTCCGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG
            361 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA
            421 TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG
            481 GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT
            541 TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT
            601 GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC
            661 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC
            721 TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT
            781 CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA
            841 TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTTCTCCC ATGAAGACGG
            901 TACGCGACTG GGCGTGGAGC ATCTGGGTCG CATTGGGGTC ACCAGCAAAT CGCGGCTGTT
            961 AGCGGGGCCC ATTTAAGTTC TGTCTCGGCC GCGTCTGCGT CTGGGCTGGC TGGCAATAAA
           1021 TATCTCACTC GGCAATCAAA TTCAGGCCGG ATTAGCGGGA ACGGAAGGCG AACTGGAGTG
          1081 GCATGATCCG GTTTCAAACA AAAACCCATG TGCCAAAAA
          //
          Primer: pGEX-ptac_UP-F ()
          Region: tac pro,GST
          Sequence file: RDB15985_A8Fid.seq check
          >D05595A3-2_A8Fi_pGEX-ptac_UP-F_D02_11_ABI24.ab1
              1 ATGGGCGGGC TATACGGTTC TGGCAATATT CTGAAATGAG CTGTTGACAA TTAATCATCG
             61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT
            121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT
            181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG
            241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG
            301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT
            361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA
            421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA
            481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC
            541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT
            601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA
            661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG
            721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT
            781 GGTTCCGCGT GGATCCGGAG GGGTCCCAGG GGCTGTTCCT GGGGGAGTTC CCGGAGGCGT
            841 CTTTTTCCCA GGGGCTGGTC TCGGAGGCCT GGGAGGAGGA GCACTGGGCC CTGGAGGCAA
            901 GCCACCCAAG CCAGGTGTCG GAGGGCTCGC AGGCGCTGGG CCTTGGGGGC AGGGCTCGGG
            961 GCCTTTCCTG CAGGCGCCCT TCCCAGGGGG CTCTGGGTGC CCGGGCGGGC GTGGCTGACG
           1021 CGGGCCGCAG CCTATAAAGC TGCTGCCAAC GCCTGCTGCG GGCCTGCCGG CCGATGGGTT
          1081 GCTGTCGGCG GCTTTAGGAG TGGTTCTTAA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Itoh, A., Small leucine-rich repeat proteoglycans associated with mature insoluble elastin serve as binding sites for galectins. Biosci. Biotechnol. Biochem. 81 (11): 2098-2104 (2017). PMID 28958189. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl