Please review the QC test results indicated by icon below as well as clone information before placing your order.

pELN-1

Bacterial expression vector of porcine elastin (ELN) long form.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15985_A8Fip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: GST-M_F () Region: GST3',thrombin site,insert5' Sequence file: RDB15985_A8Fia.seq
>D05595A3-2_A8Fi_GST-M_F_A02_02_ABI24.ab1     1 TATAGACTTT GAACTCTCAA GTTGATTTTC TTAGCAAGCT ACCTGAAATG CTGAAAATGT    61 TCGAAGATCG TTTATGTCAT AAAACATATT TAAATGGTGA TCATGTAACC CATCCTGACT   121 TCATGTTGTA TGACGCTCTT GATGTTGTTT TATACATGGA CCCAATGTGC CTGGATGCGT   181 TCCCAAAATT AGTTTGTTTT AAAAAACGTA TTGAAGCTAT CCCACAAATT GATAAGTACT   241 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG   301 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CGGAGGGGTC CCAGGGGCTG   361 TTCCTGGGGG AGTTCCCGGA GGCGTCTTTT TCCCAGGGGC TGGTCTCGGA GGCCTGGGAG   421 GAGGAGCACT GGGCCCTGGA GGCAAGCCAC CCAAGCCAGG TGTCGGAGGG CTCGCAGGCG   481 CTGGCCTTGG GGCAGGGCTC GGGGCCTTTC CTGCAGGCGC CTTCCCAGGG GCTCTGGTGC   541 CCGGCGGCGT GGCTGACGCG GCCGCAGCCT ATAAAGCTGC TGCCAAGGCT GGTGCCGGGC   601 TTGGCGGCGT TGGTGGTGTC GGCGGCTTAG GAGTGTCTAC AGGTGCAGTG GTACCTCAAC   661 TCGGAGCAGG AGTTGGAGCT GGCGCGAAGC CAGGGAAAGT ACCAGGTGTG GGGCTCCCAG   721 GTGTCTACCC TGGTGGAGTG CTCCCGGGCA CAGGAGCTCG GTTCCCAGGT GTAGGGGTGC   781 TCCCTGGCGT TCCCACTGGA ACAGGAGTCA AGGCCAAGGC CCCAGGTGGA GGTGGAGCTT   841 TTGCTGGAAT CCCAGGAGTT GGACCCTTTG GAGGTCAGCA GCCTGGAGTC CCCCCTGGGG   901 TACCCCATCA AGGCACCCAA GCTGCCAGGT GGCTACGGAC TGCCCTACAG CACTGGGAAA   961 CTGCCCTACG GCTTTGGGCC CTGGAGGAGT GGCTGGCGCC GCGGGCAAAG GCTGGGTACC  1021 CAACGGGGAC GGGGGTTTGG GTACACAAGT CTGCAGCAGC AGCAGCAGCC AGCCTAAAGG 1081 CCAGCCAGCT AAAAATATGG GTGTCCA //
Primer: pJB8-Forward () Region: insert3' Sequence file: RDB15985_A8Fib.seq
>D05595A3-2_A8Fi_pJB8-Forward_B02_05_ABI24.ab1     1 GACCATATCG CATGGTGAAC TGGGGTCATG GCTGCGCCCC GACAACCCGC CAAACACCCG    61 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG   121 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGA   181 TCGTCAGTCA GTCACGATGC GGCCGCTCGA GTCGACCCGG GAATTCTCAC TTTCTCTTCC   241 GGCCACAGGA TTTCCCCAGG CAGGCCCCAC CTTGGTATCC CAGGGCTCCC AGGGCCCCTC   301 CGAAGGGCTT GGGAGGTTTG CCACCAATTC CCAGGCCCCC AGCGCCGCCA CCTGGGAAAA   361 TAGGAGACAG TCCAAAGCCA GGCCTTGGTG CGACTCCTCC AAGTGGGAAT GGCCTGGTGA   421 CTCCTAGGAC ACCTCCGAGG CCAGCGGCAC CATATTTGGC TGCTTTAGCA GCGGCAGCCG   481 GGGACACACC TCCAAAGGCT CCAGCCCCTG GAACAGCTCC AAGGCCCCCA ACTCCCAGTC   541 CTCCGACTCC CCCGACGCCA AACTGGGCAG CTTTGGCAGC GGCTTTGGCG GCAGCAGGTC   601 CGACTCCTGC CACACCACCT GGGATACCAG CTCCGCCAAG ATCTCCAACC CCTCCAAGGG   661 CCCCGGCCGC CCCATATTTG GCTGCTTTAG CTGCGGCCAG GGGTCCAGGT ACTGCACCTG   721 CCCCAAAGCC AGGAACACCA GCACCAACTC CAAATCCAGG AACGCCGGCA CCAACTCCAA   781 ATCCGGGAAC GCCGGCAGGA AGCCCAGCAG CAGCCTGGAA CTGGGGCTTT GGCGGCTGCC   841 TTAGCAGCAG ATTTGGCTGC AGCCGGGGCC CCCTGCTCCT ATAACACCGC CAGGGCCAAT   901 GCCAATGCCG GGAGCCACAC CGACGCCGGG AGCCACACCG ACGCCGGGGA GCCACACCGA   961 CGCCGGGAGC CACACCGACG CCGGGAGCCA CACCAATGCC AGGGCCCTAA GCCGAACTGG  1021 GGCGGCTTTG GGCGGCTGCC TTTTGGCGGC CTGCAGCTGG CTGGGCTCCC TACCCCTGGC 1081 ACTGCTCCAG CCACCCGGGG CCAACCACCC C //
Primer: ColE1ori_F () Region: ori,lacI pro,lacI5' Sequence file: RDB15985_A8Fic.seq
>D05595A3-2_A8Fi_ColE1ori_F_C02_08_ABI24.ab1     1 GGTAGGTGCA TGTGGGGTTC GCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA    61 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT   121 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT   181 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG   241 TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC   301 GGTATTTCAC ACCGCATAAA TTCCGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG   361 GCATGATAGC GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA   421 TACGATGTCG CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG   481 GCCAGCCACG TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT   541 TACATTCCCA ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT   601 GCCACCTCCA GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC   661 GCCGATCAAC TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC   721 TGTAAAGCGG CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT   781 CCGCTGGATG ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA   841 TTTCTTGATG TCTCTGACCA GACACCCATC AACAGTATTA TTTTTCTCCC ATGAAGACGG   901 TACGCGACTG GGCGTGGAGC ATCTGGGTCG CATTGGGGTC ACCAGCAAAT CGCGGCTGTT   961 AGCGGGGCCC ATTTAAGTTC TGTCTCGGCC GCGTCTGCGT CTGGGCTGGC TGGCAATAAA  1021 TATCTCACTC GGCAATCAAA TTCAGGCCGG ATTAGCGGGA ACGGAAGGCG AACTGGAGTG 1081 GCATGATCCG GTTTCAAACA AAAACCCATG TGCCAAAAA //
Primer: pGEX-ptac_UP-F () Region: tac pro,GST Sequence file: RDB15985_A8Fid.seq
>D05595A3-2_A8Fi_pGEX-ptac_UP-F_D02_11_ABI24.ab1     1 ATGGGCGGGC TATACGGTTC TGGCAATATT CTGAAATGAG CTGTTGACAA TTAATCATCG    61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT   121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT   181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG   241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG   301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT   361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA   421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA   481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC   541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT   601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA   661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG   721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT   781 GGTTCCGCGT GGATCCGGAG GGGTCCCAGG GGCTGTTCCT GGGGGAGTTC CCGGAGGCGT   841 CTTTTTCCCA GGGGCTGGTC TCGGAGGCCT GGGAGGAGGA GCACTGGGCC CTGGAGGCAA   901 GCCACCCAAG CCAGGTGTCG GAGGGCTCGC AGGCGCTGGG CCTTGGGGGC AGGGCTCGGG   961 GCCTTTCCTG CAGGCGCCCT TCCCAGGGGG CTCTGGGTGC CCGGGCGGGC GTGGCTGACG  1021 CGGGCCGCAG CCTATAAAGC TGCTGCCAAC GCCTGCTGCG GGCCTGCCGG CCGATGGGTT 1081 GCTGTCGGCG GCTTTAGGAG TGGTTCTTAA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.17

GNP_filter3_RDBDEP_html_230702.pl