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pET-G4Null

Bacterial expression vector of human galectin-4 with a partially truncated linker peptide (tag-free protein).

Catalog number RDB19438
Resource name pET-G4Null
Clone info. Bacterial expression vector of human galectin-4 with a partially truncated linker peptide (tag-free protein).
Comment Expression was confirmed by the depositor with SDS-PAGE and Western blotting.
Vector backbone pET-11a (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Ampicillin
Gene/insert name human LGALS4 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
human LGALS4

          Reference sequence
            
           
          Sequence (full) RDB19438zzt01.seq compiled by us

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.


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          RDB19438 pET-G4Null DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19438_B1Kqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pET-B_F
          Region: T7 pro, lacO, RBS, insert 5'
          Sequence file: RDB19438_B1Kqa.seq check
          >D08453A5_B1Kq_2_pET-B_F_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGNCGGCN NCGATGCGTC CGGCGTANAG GANCGAGATC
             61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC
            121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCCT ATGTCCCCGC
            181 ACCGGGCTAC CAGCCCACCT ACAACCCGAC GCTGCCTTAC TACCAGCCCA TCCCGGGCGG
            241 GCTCAACGTG GGAATGTCTG TTTACATCCA AGGAGTGGCC AGCGAGCACA TGAAGCGGTT
            301 CTTCGTGAAC TTTGTGGTTG GGCAGGATCC GGGCTCAGAC GTCGCCTTCC ACTTCAATCC
            361 GCGGTTTGAC GGCTGGGACA AGGTGGTCTT CAACACGTTG CAGGGCGGGA AGTGGGGCAG
            421 CGAGGAGAGG AAGAGGAGCA TGCCCTTCAA AAAGGGTGCC GCCTTTGAGC TGGTCTTCAT
            481 AGTCCTGGCT GAGCACTACA AGGTGGTGGT AAATGGAAAT CCCTTCTATG AGTACGGGCA
            541 CCGGCTTCCC CTACAGATGG TCACCCACCT GCAAGTGGAT GGGGATCTGC AACTTCAATC
            601 AATCAACTTC ATCGGAGGCC AGCCCCTCAC CTTCAACCCG CCTGTGCCAT ATTTCGGGAG
            661 GCTGCAAGGA GGGCTCACAG CTCGAAGAAC CATCATCATC AAGGGCTATG TGCCTCCCAC
            721 AGGCAAGAGC TTTGCTATCA ACTTCAAGGT GGGCTCCTCA GGGGACATAG CTCTGCACAT
            781 TAATCNNCGC ATGGGCAACG GTACCGTGGT CCGGANNAGC CTTCTGAATG GCTCGNGGGG
            841 NNNCGANGAN AAGAAGANCA CCCACAACCC ATTTGGTCCC GGANAGNNCT TTGATCTGNN
            901 NNTNCGCTGT GGCTNNNNNC GCTTCANGGN TTANGCCNNN NNNNAGCACC NCTTTGANTT
            961 TGCCCATCNN NNNNNGGCNN TNNNNANGGN NNNNNNNTTN NNAANTNCNN GGNNNNNNNN
          1021 NN
          //
          Primer: pET-check-R1
          Region: T7 ter, insert 3'
          Sequence file: RDB19438_B1Kqb.seq check
          >D08453A5_B1Kq_2_pET-check-R1_H01_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN AACGNAGNCN GGCACCGTGN ATGAAATCTA
             61 ACAATGCGCT CATCGTCATC CTCGGCACCG TCACCCTGGA TGCTGTAGGC ATAGGCTTGG
            121 TTATGCCGGT ACTGCCGGGC CTCTTGCGGG ATATCCGGAT ATAGTTCCTC CTTTCAGCAA
            181 AAAACCCCTC AAGACCCGTT TAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT
            241 GGCAGCAGCC AACTCAGCTT CCTTTCGGGC TTTGTTAGCA GCCGGATCTT TAGATTTGGA
            301 CATAGGACAA GGTGACATCA CCCTGGATTT CCAATGTGTC CACCCTCTGG AAGGCCGAGA
            361 GGCGATGGGC AAAGTCAAAG AGGTGCTGGC CATTGGCGTA AACCTTGAAG CGATCCAAGC
            421 CACAGCGAAT GGACAGATCA AAGAACTGTC CGGGACCAAA TGGGTTGTGG GTGATCTTCT
            481 TCTCCTCGGA TCCCCACGAG CCATTCAGAA GGCTGTTCCG GACCACGGTA CCGTTGCCCA
            541 TGCGGGGATT AATGTGCAGA GCTATGTCCC CTGAGGAGCC CACCTTGAAG TTGATAGCAA
            601 AGCTCTTGCC TGTGGGAGGC ACATAGCCCT TGATGATGAT GGTTCTTCGA GCTGTGAGCC
            661 CTCCTTGCAG CCTCCCGAAA TATGGCACAG GCGGGTTGAA GGTGAGGGGC TGGCCTCCGA
            721 TGAAGTTGAN TGATTGAAGT TGCAGATCCC CATCCACTTG CAGGTGGGGT GACCATCTGT
            781 AGGGGAAGCC GGTGCCCGTA CTCATAGAAN GGATTTCCNN TTNNCACCNC CTTGTANTGC
            841 TCANCCNGGA CTATGANNAC NAGCTCAAAN NNGGCACCCT TTTTNAANNG CATGCTCCNN
            901 TTCNTNTNCT CGCTGNCCCC ACTTCCCNNC CTGCNNCGNG NTTGAANNNN NNNNTTGNCC
            961 CNNCCNNNNN AANNCGCGGN NTGAAGTGGN ANNGNNANGT NNNNANCCNN GGNNCCNNNC
          1021 NNNNNNNNNA ANNNTCNNNN NANNANCNNN NNNNNNN
          //
          Primer: lacIpro_F
          Region: lacI pro, lacI
          Sequence file: RDB19438_B1Kqc.seq check
          >D08453A5_B1Kq_2_lacIpro_F_C03_09_ABI24.ab1
              1 NNNNNNNNNN NNNNNCGNCN NNNNANANTC NATTCNGGGT GGTGAATGTG AAACCAGTAA
             61 CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC CGCGTGGTGA
            121 ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG ATGGCGGAGC
            181 TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG TTGCTGATTG
            241 GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG GCGATTAAAT
            301 CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA AGCGGCGTCG
            361 AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG CTGATCATTA
            421 ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT AATGTTCCGG
            481 CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTC TCCCATGAAG
            541 ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA ATCGCGCTGT
            601 TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT GGCTGGCTGG NATAAATATC
          661 TCNCTCGCAA TCNAATTCNN NNGANNNCNN NANGGGANNN NNANNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl