Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET-G4Null

Bacterial expression vector of human galectin-4 with a partially truncated linker peptide (tag-free protein).

Sequence (full)	RDB19438zzt01.seq compiled by us

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19438_B1Kqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, RBS, insert 5' Sequence file: RDB19438_B1Kqa.seq
>D08453A5_B1Kq_2_pET-B_F_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNCGGCN NCGATGCGTC CGGCGTANAG GANCGAGATC    61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC   121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCCT ATGTCCCCGC   181 ACCGGGCTAC CAGCCCACCT ACAACCCGAC GCTGCCTTAC TACCAGCCCA TCCCGGGCGG   241 GCTCAACGTG GGAATGTCTG TTTACATCCA AGGAGTGGCC AGCGAGCACA TGAAGCGGTT   301 CTTCGTGAAC TTTGTGGTTG GGCAGGATCC GGGCTCAGAC GTCGCCTTCC ACTTCAATCC   361 GCGGTTTGAC GGCTGGGACA AGGTGGTCTT CAACACGTTG CAGGGCGGGA AGTGGGGCAG   421 CGAGGAGAGG AAGAGGAGCA TGCCCTTCAA AAAGGGTGCC GCCTTTGAGC TGGTCTTCAT   481 AGTCCTGGCT GAGCACTACA AGGTGGTGGT AAATGGAAAT CCCTTCTATG AGTACGGGCA   541 CCGGCTTCCC CTACAGATGG TCACCCACCT GCAAGTGGAT GGGGATCTGC AACTTCAATC   601 AATCAACTTC ATCGGAGGCC AGCCCCTCAC CTTCAACCCG CCTGTGCCAT ATTTCGGGAG   661 GCTGCAAGGA GGGCTCACAG CTCGAAGAAC CATCATCATC AAGGGCTATG TGCCTCCCAC   721 AGGCAAGAGC TTTGCTATCA ACTTCAAGGT GGGCTCCTCA GGGGACATAG CTCTGCACAT   781 TAATCNNCGC ATGGGCAACG GTACCGTGGT CCGGANNAGC CTTCTGAATG GCTCGNGGGG   841 NNNCGANGAN AAGAAGANCA CCCACAACCC ATTTGGTCCC GGANAGNNCT TTGATCTGNN   901 NNTNCGCTGT GGCTNNNNNC GCTTCANGGN TTANGCCNNN NNNNAGCACC NCTTTGANTT   961 TGCCCATCNN NNNNNGGCNN TNNNNANGGN NNNNNNNTTN NNAANTNCNN GGNNNNNNNN 1021 NN //
Primer: pET-check-R1 (Pr0192) Region: T7 ter, insert 3' Sequence file: RDB19438_B1Kqb.seq
>D08453A5_B1Kq_2_pET-check-R1_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN AACGNAGNCN GGCACCGTGN ATGAAATCTA    61 ACAATGCGCT CATCGTCATC CTCGGCACCG TCACCCTGGA TGCTGTAGGC ATAGGCTTGG   121 TTATGCCGGT ACTGCCGGGC CTCTTGCGGG ATATCCGGAT ATAGTTCCTC CTTTCAGCAA   181 AAAACCCCTC AAGACCCGTT TAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT   241 GGCAGCAGCC AACTCAGCTT CCTTTCGGGC TTTGTTAGCA GCCGGATCTT TAGATTTGGA   301 CATAGGACAA GGTGACATCA CCCTGGATTT CCAATGTGTC CACCCTCTGG AAGGCCGAGA   361 GGCGATGGGC AAAGTCAAAG AGGTGCTGGC CATTGGCGTA AACCTTGAAG CGATCCAAGC   421 CACAGCGAAT GGACAGATCA AAGAACTGTC CGGGACCAAA TGGGTTGTGG GTGATCTTCT   481 TCTCCTCGGA TCCCCACGAG CCATTCAGAA GGCTGTTCCG GACCACGGTA CCGTTGCCCA   541 TGCGGGGATT AATGTGCAGA GCTATGTCCC CTGAGGAGCC CACCTTGAAG TTGATAGCAA   601 AGCTCTTGCC TGTGGGAGGC ACATAGCCCT TGATGATGAT GGTTCTTCGA GCTGTGAGCC   661 CTCCTTGCAG CCTCCCGAAA TATGGCACAG GCGGGTTGAA GGTGAGGGGC TGGCCTCCGA   721 TGAAGTTGAN TGATTGAAGT TGCAGATCCC CATCCACTTG CAGGTGGGGT GACCATCTGT   781 AGGGGAAGCC GGTGCCCGTA CTCATAGAAN GGATTTCCNN TTNNCACCNC CTTGTANTGC   841 TCANCCNGGA CTATGANNAC NAGCTCAAAN NNGGCACCCT TTTTNAANNG CATGCTCCNN   901 TTCNTNTNCT CGCTGNCCCC ACTTCCCNNC CTGCNNCGNG NTTGAANNNN NNNNTTGNCC   961 CNNCCNNNNN AANNCGCGGN NTGAAGTGGN ANNGNNANGT NNNNANCCNN GGNNCCNNNC 1021 NNNNNNNNNA ANNNTCNNNN NANNANCNNN NNNNNNN //
Primer: lacIpro_F (Pr0409) Region: lacI pro, lacI Sequence file: RDB19438_B1Kqc.seq
>D08453A5_B1Kq_2_lacIpro_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNCGNCN NNNNANANTC NATTCNGGGT GGTGAATGTG AAACCAGTAA    61 CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC CGCGTGGTGA   121 ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG ATGGCGGAGC   181 TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG TTGCTGATTG   241 GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG GCGATTAAAT   301 CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA AGCGGCGTCG   361 AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG CTGATCATTA   421 ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT AATGTTCCGG   481 CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTC TCCCATGAAG   541 ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA ATCGCGCTGT   601 TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT GGCTGGCTGG NATAAATATC 661 TCNCTCGCAA TCNAATTCNN NNGANNNCNN NANGGGANNN NNANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl