Mouse cDNA for IgE L chain kappa.
Alternative name | Mouse IgE L chain |
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Clone info. | Mouse cDNA for IgE L chain kappa in pGEM-T Easy vector. Please note that this clone contains no signal peptide sequence. |
Vector backbone | pGEM-T Easy (plasmid) |
Size of vector backbone | 3.0 kb |
Selectable markers | Amp^r |
Gene/insert name | mouse IgE L chain kappa cDNA |
Reference of insert sequence | LC031495 (DDBJ accession number) |
Depositor|Developer | Nishi, Nozomu | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017). |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15984 | mIGEL | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The mIGEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15984). |
Reference section:
Nakakita, S., Itoh, A., Nakakita, Y., Nonaka, Y., Ogawa, T., Nakamura, T., Nishi, N., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15984_A8Fip1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 () Region: insert5' Sequence file: RDB15984_A8Fia.seq |
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>D05595A2-3_A8Fi_M13_-40_D01_04_ABI08.ab1 1 AGGCAAGGTG GATTGTTAAT ACGACTCACT ATAGGGCGAA TTGGGCCCGA CGTCGCATGC 61 TCCCGGCCGC CATGGCGGCC GCGGGAATTC GATTGATGTT TTGATGACCC AAACTCCACT 121 CTCCCTGCCT GTCAGTCTTG GAGATCAAGC CTCCATCTCT TGCAGATCTA GTCAGAGCAT 181 TGTACATAGT AATGGAAACA CCTATTTAGA ATGGTACCTG CAGAAACCAG GCCAGTCTCC 241 AAAGCTCCTG ATCTACAAAG TTTCCAACCG ATTTTCTGGG GTCCCAGACA GGTTCAGTGG 301 CAGTGGATCA GGGACAGATT TCACACTCAA GATCAGCAGA GTGGAGGCTG AGGATCTGGG 361 AGTTTATTAC TGCTTTCAAG GTTCACATGT TCCGCTCACG TTCGGTGCTG GGACCAAGCT 421 GGAGCTGAAA CGGGCTGATG CTGCACCAAC TGTATCCATC TTCCCACCAT CCAGTGAGCA 481 GTTAACATCT GGAGGTGCCT CAGTCGTGTG CTTCTTGAAC AACTTCTACC CCAAAGACAT 541 CAATGTCAAG TGGAAGATTG ATGGCAGTGA ACGACAAAAT GGCGTCCTGA ACAGTTGGAC 601 TGATCAGGAC AGCAAAGACA GCACCTACAG CATGAGCAGC ACCCTCACGT TGACCAAGGA 661 CGAGTATGAA CGACATAACA GCTATACCTG TGAGGCCACT CACAAGACAT CAACTTCACC 721 CATTGTCAAG AGCTTCAACA GGAATGAGTG TTAGAATCAC TAGTGAATTC GCGGCCGCCT 781 GCAGGTCGAC CATATGGGAG AGCTCCCAAC GCGTTGGATG CATAGCTTGA GTATTCTATA 841 GTGTCACCTA AATAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 901 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 961 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 1021 AAACCTGTCG TGCCCAGCTG CATTAATGAA TCGGGCCAAC GCGCGGGGGA GAGGCGGGAT 1081 TGCGTATGGG CGCTCTTCCG CTTTCCTCGC TTCACTGAAC TTCGCTGGCG CTCGGGCTCG 1141 GATTCGCTGG CTGTCGAGGC GGGATATATC CAGCTCA // |
Primer: ColE1ori_F () Region: ori, insert3' Sequence file: RDB15984_A8Fib.seq |
>D05595A2-3_A8Fi_ColE1ori_F_G01_19_ABI24.ab1 1 GGGGGTGGCA GGGCGGGGTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 61 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 121 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 181 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 241 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 301 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 361 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 421 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA 481 TGACCATGAT TACGCCAAGC TATTTAGGTG ACACTATAGA ATACTCAAGC TATGCATCCA 541 ACGCGTTGGG AGCTCTCCCA TATGGTCGAC CTGCAGGCGG CCGCGAATTC ACTAGTGATT 601 CTAACACTCA TTCCTGTTGA AGCTCTTGAC AATGGGTGAA GTTGATGTCT TGTGAGTGGC 661 CTCACAGGTA TAGCTGTTAT GTCGTTCATA CTCGTCCTTG GTCAACGTGA GGGTGCTGCT 721 CATGCTGTAG GTGCTGTCTT TGCTGTCCTG ATCAGTCCAA CTGTTCAGGA CGCCATTTTG 781 TCGTTCACTG CCATCAATCT TCCACTTGAC ATTGATGTCT TTGGGGTAGA AAGTTGTTCA 841 AGAAAGCACA CGACTGAGGC ACCTCCAGGA TGTTAACTGC TCACTGGGAT GGTGGGAAAG 901 ATGGAATACA AGTTTGGTGC AGCAATCAGC CCCGTTTCAA GCTTCCAGCC TTGGGTCCCC 961 AGCACCGAAC CGGTGAGGCG GGAAACATGT GGAAGCCCTT GGAAAGGCAG GTAATAGACT 1021 TCCCCAGAAT TCCATCAAGC CTCCAACTTC CTGGCTGGAT ATCTTGGAAG TGGTGGAAAA 1081 TTCCTGATCC CCTTGGAATC CAA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205. [link to RRC of NBRP] |
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