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mIGEL

Mouse cDNA for IgE L chain kappa.

Catalog number RDB15984
Resource name mIGEL
Alternative name Mouse IgE L chain
Clone info. Mouse cDNA for IgE L chain kappa in pGEM-T Easy vector. Please note that this clone contains no signal peptide sequence.
Vector backbone pGEM-T Easy (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse IgE L chain kappa cDNA
Reference of nucleotide sequence LC031495 (DDBJ accession number)
Depositor Nishi, Nozomu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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RDB15984 mIGEL DNA solution

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References and tips

Electronic file

Original reference

original Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15984_A8Fip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: insert5' | Primer: M13_-40 | Sequence: RDB15984_A8Fia.seq
>D05595A2-3_A8Fi_M13_-40_D01_04_ABI08.ab1
    1 AGGCAAGGTG GATTGTTAAT ACGACTCACT ATAGGGCGAA TTGGGCCCGA CGTCGCATGC
   61 TCCCGGCCGC CATGGCGGCC GCGGGAATTC GATTGATGTT TTGATGACCC AAACTCCACT
  121 CTCCCTGCCT GTCAGTCTTG GAGATCAAGC CTCCATCTCT TGCAGATCTA GTCAGAGCAT
  181 TGTACATAGT AATGGAAACA CCTATTTAGA ATGGTACCTG CAGAAACCAG GCCAGTCTCC
  241 AAAGCTCCTG ATCTACAAAG TTTCCAACCG ATTTTCTGGG GTCCCAGACA GGTTCAGTGG
  301 CAGTGGATCA GGGACAGATT TCACACTCAA GATCAGCAGA GTGGAGGCTG AGGATCTGGG
  361 AGTTTATTAC TGCTTTCAAG GTTCACATGT TCCGCTCACG TTCGGTGCTG GGACCAAGCT
  421 GGAGCTGAAA CGGGCTGATG CTGCACCAAC TGTATCCATC TTCCCACCAT CCAGTGAGCA
  481 GTTAACATCT GGAGGTGCCT CAGTCGTGTG CTTCTTGAAC AACTTCTACC CCAAAGACAT
  541 CAATGTCAAG TGGAAGATTG ATGGCAGTGA ACGACAAAAT GGCGTCCTGA ACAGTTGGAC
  601 TGATCAGGAC AGCAAAGACA GCACCTACAG CATGAGCAGC ACCCTCACGT TGACCAAGGA
  661 CGAGTATGAA CGACATAACA GCTATACCTG TGAGGCCACT CACAAGACAT CAACTTCACC
  721 CATTGTCAAG AGCTTCAACA GGAATGAGTG TTAGAATCAC TAGTGAATTC GCGGCCGCCT
  781 GCAGGTCGAC CATATGGGAG AGCTCCCAAC GCGTTGGATG CATAGCTTGA GTATTCTATA
  841 GTGTCACCTA AATAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
  901 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
  961 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
 1021 AAACCTGTCG TGCCCAGCTG CATTAATGAA TCGGGCCAAC GCGCGGGGGA GAGGCGGGAT
 1081 TGCGTATGGG CGCTCTTCCG CTTTCCTCGC TTCACTGAAC TTCGCTGGCG CTCGGGCTCG
1141 GATTCGCTGG CTGTCGAGGC GGGATATATC CAGCTCA
//
Region: ori, insert3' | Primer: ColE1ori_F | Sequence: RDB15984_A8Fib.seq
>D05595A2-3_A8Fi_ColE1ori_F_G01_19_ABI24.ab1
    1 GGGGGTGGCA GGGCGGGGTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC
   61 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT
  121 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG
  181 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA
  241 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG
  301 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG
  361 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT
  421 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA
  481 TGACCATGAT TACGCCAAGC TATTTAGGTG ACACTATAGA ATACTCAAGC TATGCATCCA
  541 ACGCGTTGGG AGCTCTCCCA TATGGTCGAC CTGCAGGCGG CCGCGAATTC ACTAGTGATT
  601 CTAACACTCA TTCCTGTTGA AGCTCTTGAC AATGGGTGAA GTTGATGTCT TGTGAGTGGC
  661 CTCACAGGTA TAGCTGTTAT GTCGTTCATA CTCGTCCTTG GTCAACGTGA GGGTGCTGCT
  721 CATGCTGTAG GTGCTGTCTT TGCTGTCCTG ATCAGTCCAA CTGTTCAGGA CGCCATTTTG
  781 TCGTTCACTG CCATCAATCT TCCACTTGAC ATTGATGTCT TTGGGGTAGA AAGTTGTTCA
  841 AGAAAGCACA CGACTGAGGC ACCTCCAGGA TGTTAACTGC TCACTGGGAT GGTGGGAAAG
  901 ATGGAATACA AGTTTGGTGC AGCAATCAGC CCCGTTTCAA GCTTCCAGCC TTGGGTCCCC
  961 AGCACCGAAC CGGTGAGGCG GGAAACATGT GGAAGCCCTT GGAAAGGCAG GTAATAGACT
 1021 TCCCCAGAAT TCCATCAAGC CTCCAACTTC CTGGCTGGAT ATCTTGGAAG TGGTGGAAAA
1081 TTCCTGATCC CCTTGGAATC CAA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205.

2019.09.06

GNP_filter3_RDBDEP_html_190816.pl