Bacteria expression vector of human galectin-12 with an N-terminally extended NCRD (LNG12), GST fusion protein
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External Database Human lectin, galactoside-binding, soluble, 12 Reference sequence |
Remarks, protocol and/or map (pdf) | RDB04225.pdf |
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Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB04225 | pGEX-LNG12-3 | DNA solution | Please contact us. |
Materials & Methods section:
The pGEX-LNG12-3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04225). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15792_A8Bep1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pGEX-F2 Sequence file: RDB15792_A8Bea.seq |
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>04225_15792_A8Be_pGEX-F2_B02_05_ABI24.ab1 1 CATATTAAGT TTTGGTTTTT AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTACTT 61 GAAATCCAGC AAGTATATAG CATGGCCTTT GCAGGGCTGG CAAGCCACGT TTGGTGGTGG 121 CGACCATCCT CCAAAATCGG ATCTGGTTCC GCGTGGATCC ATGAGTCAGC CCAGTGGGGG 181 CAGGGCTCCT GGAACGAGGA TCTACAGTTG GAGTTGCCCC ACTGTCATGT CACCTGGAGA 241 AAAACTGGAC CCAATTCCTG ACAGCTTCAT TCTGCAACCA CCAGTCTTCC ACCCGGTGGT 301 TCCTTATGTC ACGACGATTT TTGGAGGCCT GCATGCAGGC AAGATGGTCA TGCTGCAAGG 361 AGTGGTCCCT CTAGATGCAC ACAGGTTTCA GGTGGACTTC CAGTGTGGCT GCAGCCTGTG 421 TCCCCGGCCA GATATCGCCT TCCACTTCAA CCCTCGCTTC CATACCACCA AGCCCCATGT 481 CATCTGCAAC ACCCTGCATG GTGGACGCTG GCAAAGGGAG GCCCGGTGGC CCCACCTGGC 541 CCTGCGAAGA GGCTCCAGCT TCCTCATCCT CTTTCTCTTC GGGAATGAGG AAGTGAAGGT 601 GAGTGTGAAT GGACAGCACT TTCTCCACTT CCGCTACCGG CTCCCACTGT CTCATGTGGA 661 CACGCTGGGT ATATTTGGTG ACATCCTGGT AGAGGCTGTT GGATTCCTGA ACATCAATCC 721 ATTTGTGGAG GGCAGCAGAG AGTACCCAGC TGGACATCCT TTCCTGCTGA TGAGCCCCAG 781 GCTGGAGGTG CCCTGCTCAC ATGCTCTTCC CCAGGGTCTC TCGCCTGGGG CAGGTCATCA 841 TAGTACGGGG ACTGGTCTTG CAAGAGCCGA AGCATTTTAC TGTGAGCCTG AGGGGACCAG 901 GCTGCCCATG CTCCTGTGAC ACTCAGGGCC TCCTTCGCAG ACAGAACTCT GGGCCTGGAT 961 CTCCCGCTGG GGGGCAGAAG AAACTGATCT CAGCCCCCTT TCCTCTTTTA CCCCCAGAGA 1021 TTCTTTTGGA GGTGGCTGCT CCTGGTTCCA GGAGGGAGGG CTGAAGGCTG GGCGCTCAAT 1081 GGGGCAGGGG CTGGGGGGGC ATCAGCATGG AACAGCAGTC TTGGAGCAGC TGGCGGGGAG 1141 CTTCGGAATC AGTGGGAAGG GTGTCAGAC // |
Primer: pGEX-RSEQ Sequence file: RDB15792_A8Beb.seq |
>04225_15792_A8Be_pGEX-RSEQ_C02_08_ABI24.ab1 1 ACACAAAACG GAAACGGCGC GAGGCAGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT 61 CGACCCGGGA ATTCTCAGGA GTGGACACAG TAGAGCTGGA CACTTCCACT GATCCGGAGC 121 TCCCGCAGCT GCTCCAGGGC CTGCTGGTTC ATGCTGGTGG CCCCCAGCCC CTGCCCATTG 181 AGCGCCAGCT TCAGCCCTCC CTCCTGGAAC AGGAGCAGCA CCTCAAAGAA TCTCTGGGGG 241 TAAAAGAGGA AGGGGGCTGA GATCAGTTTC TTCTGCCCCC AGCGGGAGAT CCAGGCCAGA 301 GTTCTGTCTG CGAAGGAGGC CCTGAGTGTC ACAGGAGCAT GGGCAGCCTG GTCCCTCAGG 361 CTCACAGTAA AATGCTTCGG CTCTTGCAAG ACCAGTCCCC GTACTATGAT GACCTGCCCA 421 GGCGAGAGAC CCTGGGGAAG AGCATGTGAC AGGGCACCTC CAGCCTGGGG CTCATCACAG 481 AAAGGATGTC CAGCTGGGAC TCTCTGCTGC CCTCCCAATG GATTGTGGTC AGGATCCAAC 541 GCCTCTACCA GAGTCACCAA TATACCCGCG TGTCACATAA AAATGGAACC GGTACGAAGT 601 GAAAAGGGTG GCATTCAATC ACTTAATCCT CATCCGAAAA AAAAGAAAAA AAATGAGCTC 661 TCCAGGCCAG GGGCACGGCT CCTGCCGTAC ATGGGGTTCA TAATGGCTGG ATGACAGTTA 721 ATAAGAATGG CGGACAGTTC CCATGAACCT AGACTGGATT AAGGACCTTA ATAACTTGCG 781 AGAGCTTAAT TTGGGATGGA CCCGGAGACT GTGCCAGACT TTTAAAAAGA GCATTTCCAC 841 GACAGAGATA CTCAGATCCT CCCAAAGATC GTCTTTGGTA AATGGGAAGC GGCCACTCAA 901 TAGATGGGAG AAGC // |
Primer: M13_-40 Sequence file: RDB15792_A8Bec.seq |
>04225_15792_A8Be_M13_-40_D02_11_ABI24.ab1 1 ACCAAGTTAA CCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 61 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 121 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 181 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 241 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT 301 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT 361 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA 421 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA 481 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT 541 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT 601 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG 661 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA 721 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG 781 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT 841 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA 901 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC 961 CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA 1021 GACTGGAGTG GCACGCCAAT CAGCCACGAC TGTTTGCCCG CCAGTTGTGT GCACGCCGCT 1081 GGGATGTATT CAGCCTCCGG CATCGGCGGC TCAACTTTTT CCCGCGTTTC GGCAGAAACG 1141 GTGTGCCTGT GGATCCTTG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
protocol | Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP] |
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2023.08.17
GNP_filter3_RDBDEP_html_230702.pl