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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET-mG1-1a

Bacteria expression vector of mouse galectin-1, tag-free protein

Catalog number RDB08434
Resource name pET-mG1-1a
Clone info. Bacterial expression vector of mouse galectin-1 (tag-free protein).
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pET-11a (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse galectin-1 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Mouse galectin-1

          Reference sequence
            
           
          Sequence (full) RDB08434zzt01.seq compiled by us

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
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          RDB08434 pET-mG1-1a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19462_B1Kqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pET-B_F
          Region: T7 pro, lacO, insert, T7 ter
          Sequence file: RDB19462_B1Kqa.seq check
          >D08453A9_B1Kq_1_pET-B_F_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNGNCNGN NNNGNCGNCC NCGATGCGTC CGGCGTAGAG GANCGAGATC
             61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC
            121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCCT GTGGTCTGGT
            181 CGCCAGCAAC CTGAATCTCA AACCTGGGGA ATGTCTCAAA GTTCGGGGAG AGGTGGCCTC
            241 GGACGCCAAG AGCTTTGTGC TGAACCTGGG AAAAGACAGC AACAACCTGT GCCTACACTT
            301 CAATCCTCGC TTCAATGCCC ATGGAGACGC CAACACCATT GTGTGTAACA CCAAGGAAGA
            361 TGGGACCTGG GGAACCGAAC ACCGGGAACC TGCCTTCCCC TTCCAGCCCG GGAGCATCAC
            421 AGAGGTGTGC ATCACCTTTG ACCAGGCTGA CCTGACCATC AAGCTGCCAG ACGGACATGA
            481 ATTCAAGTTC CCCAACCGCC TCAACATGGA GGCCATCAAC TACATGGCGG CGGATGGAGA
            541 CTTCAAGATT AAGTGCGTGG CCTTTGAGTG AGGATCCGGC TGCTAACAAA GCCCGAAAGG
            601 AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA
            661 AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCCGCAAGAG
            721 GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG
            781 GANGANGATG ANCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA
            841 CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GANAATTCTT
            901 GAAGANNAAN GGNNCTCNNG ANACGCCNAT TTTTATAGGT TANNGNCNTG ATAATAATGN
          961 NTTCNNANAN GTCNNNNGGC NCTTTTCGGG GAAATGNNNN NNNNNN
          //
          Primer: pET-check-R1
          Region: T7 ter, insert, lacO, T7 pro
          Sequence file: RDB19462_B1Kqb.seq check
          >D08453A9_B1Kq_1_pET-check-R1_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNAACGC NGNCNGGCNC CGTGTATGAA ATCTAACAAT
             61 GCGCTCATCG TCATCCTCGG CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG
            121 CCGGTACTGC CGGGCCTCTT GCGGGATATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC
            181 CCCTCAAGAC CCGTTTAGAG GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG
            241 CAGCCAACTC AGCTTCCTTT CGGGCTTTGT TAGCAGCCGG ATCCTCACTC AAAGGCCACG
            301 CACTTAATCT TGAAGTCTCC ATCCGCCGCC ATGTAGTTGA TGGCCTCCAT GTTGAGGCGG
            361 TTGGGGAACT TGAATTCATG TCCGTCTGGC AGCTTGATGG TCAGGTCAGC CTGGTCAAAG
            421 GTGATGCACA CCTCTGTGAT GCTCCCGGGC TGGAAGGGGA AGGCAGGTTC CCGGTGTTCG
            481 GTTCCCCAGG TCCCATCTTC CTTGGTGTTA CACACAATGG TGTTGGCGTC TCCATGGGCA
            541 TTGAAGCGAG GATTGAAGTG TAGGCACAGG TTGTTGCTGT CTTTTCCCAG GTTCAGCACA
            601 AAGCTCTTGG CGTCCGAGGC CACCTCTCCC CGAACTTTGA GACATTCCCC AGGTTTGAGA
            661 TTCAGGTTGC TGGCGACCAG ACCACAGGCC ATATGTATAT CTCCTTCTTA AAGTTAAACA
            721 AAATTATTTC TAGAGGGGAA TTGTTATCCG CTCACAATTC CCCTATAGTG AGTCGTATTA
            781 ATTTCGCGGG ATCGAGATCT CGATCCTCTA CGCCGGANGC ATCGNGGCCG GCATCACCGG
            841 CGCCNCANGT GCGGNTGCTG GCGCCTATAT CGCCGANATC NCCGATNGGG AAGATCGGGC
            901 TCGNCACTTC NGGCTNATGA NCGCTNGTTT CGGCGNGGGT ATGGNGGCAN NNCCNNNNNN
          961 NGGGGGANTG NNGGGCGNNN TCNNCNTNGC NTGNACNNTN NCTNNNNNNN NNN
          //
          Primer: lacIpro_F
          Region: lacI pro, lacI
          Sequence file: RDB19462_B1Kqc.seq check
          >D08453A9_B1Kq_1_lacIpro_F_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNGCGNCNG GNNANANTCN NTTCNGGGTG GTGAATGTGA AACCAGTAAC
             61 GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC GCGTGGTGAA
            121 CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT
            181 GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT TGCTGATTGG
            241 CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG CGATTAAATC
            301 TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA GCGGCGTCGA
            361 AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC TGATCATTAA
            421 CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA ATGTTCCGGC
            481 GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT CCCATGAAGA
            541 CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA TCGCGCTGTT
            601 AGCGGGCCCN TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GNTGGNTGGN ATAAATATCT
          661 NACTCNNAAT CNANNTCNNC NNANANCGGN NNNGGNANNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl