RDB08434 - pET-mG1-1a

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icon below as well as clone information before placing your order.

pET-mG1-1a (#RDB08434)

Bacteria expression vector of mouse galectin-1, tag-free protein

Clone info.	Bacterial expression vector of mouse galectin-1 (tag-free protein).
Comment	Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone	pET-11a (Plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	Mouse galectin-1 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Sequence (full)	RDB08434zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、学術機関の学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08434	pET-mG1-1a	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-mG1-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08434).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19462_B1Kqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, insert, T7 ter Sequence file: RDB19462_B1Kqa.seq
>D08453A9_B1Kq_1_pET-B_F_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNNGNCNGN NNNGNCGNCC NCGATGCGTC CGGCGTAGAG GANCGAGATC 61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC 121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCCT GTGGTCTGGT 181 CGCCAGCAAC CTGAATCTCA AACCTGGGGA ATGTCTCAAA GTTCGGGGAG AGGTGGCCTC 241 GGACGCCAAG AGCTTTGTGC TGAACCTGGG AAAAGACAGC AACAACCTGT GCCTACACTT 301 CAATCCTCGC TTCAATGCCC ATGGAGACGC CAACACCATT GTGTGTAACA CCAAGGAAGA 361 TGGGACCTGG GGAACCGAAC ACCGGGAACC TGCCTTCCCC TTCCAGCCCG GGAGCATCAC 421 AGAGGTGTGC ATCACCTTTG ACCAGGCTGA CCTGACCATC AAGCTGCCAG ACGGACATGA 481 ATTCAAGTTC CCCAACCGCC TCAACATGGA GGCCATCAAC TACATGGCGG CGGATGGAGA 541 CTTCAAGATT AAGTGCGTGG CCTTTGAGTG AGGATCCGGC TGCTAACAAA GCCCGAAAGG 601 AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA 661 AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCCGCAAGAG 721 GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG 781 GANGANGATG ANCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA 841 CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GANAATTCTT 901 GAAGANNAAN GGNNCTCNNG ANACGCCNAT TTTTATAGGT TANNGNCNTG ATAATAATGN 961 NTTCNNANAN GTCNNNNGGC NCTTTTCGGG GAAATGNNNN NNNNNN //
Primer: pET-check-R1 (Pr0192) Region: T7 ter, insert, lacO, T7 pro Sequence file: RDB19462_B1Kqb.seq
>D08453A9_B1Kq_1_pET-check-R1_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNAACGC NGNCNGGCNC CGTGTATGAA ATCTAACAAT 61 GCGCTCATCG TCATCCTCGG CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG 121 CCGGTACTGC CGGGCCTCTT GCGGGATATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC 181 CCCTCAAGAC CCGTTTAGAG GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG 241 CAGCCAACTC AGCTTCCTTT CGGGCTTTGT TAGCAGCCGG ATCCTCACTC AAAGGCCACG 301 CACTTAATCT TGAAGTCTCC ATCCGCCGCC ATGTAGTTGA TGGCCTCCAT GTTGAGGCGG 361 TTGGGGAACT TGAATTCATG TCCGTCTGGC AGCTTGATGG TCAGGTCAGC CTGGTCAAAG 421 GTGATGCACA CCTCTGTGAT GCTCCCGGGC TGGAAGGGGA AGGCAGGTTC CCGGTGTTCG 481 GTTCCCCAGG TCCCATCTTC CTTGGTGTTA CACACAATGG TGTTGGCGTC TCCATGGGCA 541 TTGAAGCGAG GATTGAAGTG TAGGCACAGG TTGTTGCTGT CTTTTCCCAG GTTCAGCACA 601 AAGCTCTTGG CGTCCGAGGC CACCTCTCCC CGAACTTTGA GACATTCCCC AGGTTTGAGA 661 TTCAGGTTGC TGGCGACCAG ACCACAGGCC ATATGTATAT CTCCTTCTTA AAGTTAAACA 721 AAATTATTTC TAGAGGGGAA TTGTTATCCG CTCACAATTC CCCTATAGTG AGTCGTATTA 781 ATTTCGCGGG ATCGAGATCT CGATCCTCTA CGCCGGANGC ATCGNGGCCG GCATCACCGG 841 CGCCNCANGT GCGGNTGCTG GCGCCTATAT CGCCGANATC NCCGATNGGG AAGATCGGGC 901 TCGNCACTTC NGGCTNATGA NCGCTNGTTT CGGCGNGGGT ATGGNGGCAN NNCCNNNNNN 961 NGGGGGANTG NNGGGCGNNN TCNNCNTNGC NTGNACNNTN NCTNNNNNNN NNN //
Primer: lacIpro_F (Pr0409) Region: lacI pro, lacI Sequence file: RDB19462_B1Kqc.seq
>D08453A9_B1Kq_1_lacIpro_F_D03_12_ABI24.ab1 1 NNNNNNNNNN NNNGCGNCNG GNNANANTCN NTTCNGGGTG GTGAATGTGA AACCAGTAAC 61 GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC GCGTGGTGAA 121 CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT 181 GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT TGCTGATTGG 241 CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG CGATTAAATC 301 TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA GCGGCGTCGA 361 AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC TGATCATTAA 421 CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA ATGTTCCGGC 481 GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT CCCATGAAGA 541 CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA TCGCGCTGTT 601 AGCGGGCCCN TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GNTGGNTGGN ATAAATATCT 661 NACTCNNAAT CNANNTCNNC NNANANCGGN NNNGGNANNN NNNNNNN //