Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET-mG1-1a

Bacteria expression vector of mouse galectin-1, tag-free protein

Sequence (full)	RDB08434zzt01.seq compiled by us

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19462_B1Kqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, insert, T7 ter Sequence file: RDB19462_B1Kqa.seq
>D08453A9_B1Kq_1_pET-B_F_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNGNCNGN NNNGNCGNCC NCGATGCGTC CGGCGTAGAG GANCGAGATC    61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC   121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCCT GTGGTCTGGT   181 CGCCAGCAAC CTGAATCTCA AACCTGGGGA ATGTCTCAAA GTTCGGGGAG AGGTGGCCTC   241 GGACGCCAAG AGCTTTGTGC TGAACCTGGG AAAAGACAGC AACAACCTGT GCCTACACTT   301 CAATCCTCGC TTCAATGCCC ATGGAGACGC CAACACCATT GTGTGTAACA CCAAGGAAGA   361 TGGGACCTGG GGAACCGAAC ACCGGGAACC TGCCTTCCCC TTCCAGCCCG GGAGCATCAC   421 AGAGGTGTGC ATCACCTTTG ACCAGGCTGA CCTGACCATC AAGCTGCCAG ACGGACATGA   481 ATTCAAGTTC CCCAACCGCC TCAACATGGA GGCCATCAAC TACATGGCGG CGGATGGAGA   541 CTTCAAGATT AAGTGCGTGG CCTTTGAGTG AGGATCCGGC TGCTAACAAA GCCCGAAAGG   601 AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA   661 AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCCGCAAGAG   721 GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG   781 GANGANGATG ANCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA   841 CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GANAATTCTT   901 GAAGANNAAN GGNNCTCNNG ANACGCCNAT TTTTATAGGT TANNGNCNTG ATAATAATGN 961 NTTCNNANAN GTCNNNNGGC NCTTTTCGGG GAAATGNNNN NNNNNN //
Primer: pET-check-R1 (Pr0192) Region: T7 ter, insert, lacO, T7 pro Sequence file: RDB19462_B1Kqb.seq
>D08453A9_B1Kq_1_pET-check-R1_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNAACGC NGNCNGGCNC CGTGTATGAA ATCTAACAAT    61 GCGCTCATCG TCATCCTCGG CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG   121 CCGGTACTGC CGGGCCTCTT GCGGGATATC CGGATATAGT TCCTCCTTTC AGCAAAAAAC   181 CCCTCAAGAC CCGTTTAGAG GCCCCAAGGG GTTATGCTAG TTATTGCTCA GCGGTGGCAG   241 CAGCCAACTC AGCTTCCTTT CGGGCTTTGT TAGCAGCCGG ATCCTCACTC AAAGGCCACG   301 CACTTAATCT TGAAGTCTCC ATCCGCCGCC ATGTAGTTGA TGGCCTCCAT GTTGAGGCGG   361 TTGGGGAACT TGAATTCATG TCCGTCTGGC AGCTTGATGG TCAGGTCAGC CTGGTCAAAG   421 GTGATGCACA CCTCTGTGAT GCTCCCGGGC TGGAAGGGGA AGGCAGGTTC CCGGTGTTCG   481 GTTCCCCAGG TCCCATCTTC CTTGGTGTTA CACACAATGG TGTTGGCGTC TCCATGGGCA   541 TTGAAGCGAG GATTGAAGTG TAGGCACAGG TTGTTGCTGT CTTTTCCCAG GTTCAGCACA   601 AAGCTCTTGG CGTCCGAGGC CACCTCTCCC CGAACTTTGA GACATTCCCC AGGTTTGAGA   661 TTCAGGTTGC TGGCGACCAG ACCACAGGCC ATATGTATAT CTCCTTCTTA AAGTTAAACA   721 AAATTATTTC TAGAGGGGAA TTGTTATCCG CTCACAATTC CCCTATAGTG AGTCGTATTA   781 ATTTCGCGGG ATCGAGATCT CGATCCTCTA CGCCGGANGC ATCGNGGCCG GCATCACCGG   841 CGCCNCANGT GCGGNTGCTG GCGCCTATAT CGCCGANATC NCCGATNGGG AAGATCGGGC   901 TCGNCACTTC NGGCTNATGA NCGCTNGTTT CGGCGNGGGT ATGGNGGCAN NNCCNNNNNN 961 NGGGGGANTG NNGGGCGNNN TCNNCNTNGC NTGNACNNTN NCTNNNNNNN NNN //
Primer: lacIpro_F (Pr0409) Region: lacI pro, lacI Sequence file: RDB19462_B1Kqc.seq
>D08453A9_B1Kq_1_lacIpro_F_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNGCGNCNG GNNANANTCN NTTCNGGGTG GTGAATGTGA AACCAGTAAC    61 GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC GCGTGGTGAA   121 CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT   181 GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT TGCTGATTGG   241 CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG CGATTAAATC   301 TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG GTAGAACGAA GCGGCGTCGA   361 AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC GTCAGTGGGC TGATCATTAA   421 CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT GCCTGCACTA ATGTTCCGGC   481 GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT ATTATTTTCT CCCATGAAGA   541 CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT CACCAGCAAA TCGCGCTGTT   601 AGCGGGCCCN TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG GNTGGNTGGN ATAAATATCT 661 NACTCNNAAT CNANNTCNNC NNANANCGGN NNNGGNANNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl