RDB08415 - pGEX-G9(S)-1a

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pGEX-G9(S)-1a

Bacteria expression vector of human galectin-9 with a short linker peptide (G9(S)/G9S), GST fusion protein

Catalog number	RDB08415
Resource name	pGEX-G9(S)-1a
Clone info.	Bacterial expression clone of human galectin-9 (short linker type).
Comment	Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone	pGEX-4T-2 (Plasmid)
Size of vector backbone	5.0 kb
Selectable markers	Amp^r
Gene/insert name	Human galectin-9 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Other clones in our bank

human galectin-9

External Database
Human galectin-9

Reference sequence

Distribution information

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Terms and conditions set forth by the DEPOSITOR	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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RDB08415	pGEX-G9(S)-1a	DNA solution

Please review the QC test results indicated by check

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References and tips

Electronic file

Sequence	RDB08415z.seq

Original reference

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08415_A4E9.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_F Sequence file: RDB08415_A4E9a.seq
>08415_08415_A4E9_pGEX_F_G04_19.ab1 1 GGCCATCATC AAATCGGATC TGGTTCCGCG TGGATCCCCA GGAATTCCCA TGGCCTTCAG 61 CGGTTCCCAG GCTCCCAACC TGAGTCCAGC TGTCCCCTTT TCTGGGACTA TTCAAGGAGG 121 TCTCCAGGAC GGACTTCAGA TCACTGTCAA TGGGACCGTT CTCAGCTCCA GTGGAACCAG 181 GTTTGCTGTG AACTTTCAGA CTGGCTTCAG TGGAAATGAC ATTGCCTTCC ACTTCAACCC 241 TCGGTTTGAA GATGGAGGGT ACGTGGTGTG CAACACGAGG CAGAACGGAA GCTGGGGGCC 301 CGAGGAGAGG AAGACACACA TGCCTTTCCA GAAGGGGATG CCCTTTGACC TCTGCTTCCT 361 GGTGCAGAGC TCAGATTTCA AGGTGATGGT GAACGGGATC CTCTTCGTGC AGTACTTCCA 421 CCGCGTGCCC TTCCACCGTG TGGACACCAT CTCCGTCAAT GGCTCTGTGC AGCTGTCCTA 481 CATCAGCTTC CAGACCCAGA CAGTCATCCA CACAGTGCAG AGCGCCCCTG GACAGATGTT 541 CTCTACTCCC GCCATCCCAC CTATGATGTA CCCCCACCCC GCCTATCCGA TGCCTTTCAT 601 CACCACCATT CTGGGAGGGC TGTACCCATC CAAGTCCATC CTCCTGTCAG GCACTGTCCT 661 GCCCAGTGCT CAGAGGTTCC ACATCAACCT GTGCTCTGGG AACCACATCG CCTTCCACCT 721 GAACCCCCGT TTTGATGAGA ATGCTGTGGT CCGCAACACC CAGATCGACA ACTCCTGGGG 781 GTCTGAGGAG CGAAGTCTGC CCCGAAAAAT GCCCTTCGTC CGTGGCCAGA GCTTCTCAGT 841 GTGGATCTTG TGTGAAGCTC ACTGCCTCAA GGTGGCCGTG GATGGTCAGC ACCTGTTTGA 901 ATACTACCAT CGCCTGAGGA ACCTGCCCAC CATCAACAGA CTGGAAGTGG GGGGGCGACA 961 TCCAGCTGAC CCATGTGCAG ACATAGCTCG AGCGGGCCGC ATCGTGACTG ACTGACGATC 1021 TGCCTCGCGC GTTTCGGTGA TGACGTGAAA ACCTCTGACA CATGCAGCTC CGGGAGACGG 1081 TCACAGCTTG TCTGTAGCCG GATGCTGGAG CAGAACTAGG CCCGTTCAGG CGCCGGTTCA 1141 AGCCGGGGTG TTTGGGC //
Primer: pGEX_R Sequence file: RDB08415_A4E9b.seq
>08415_08415_A4E9_pGEX_R_H04_22.ab1 1 GCCATTACCG GAAACGCGGC CGGAGGCAGA TCGTCAGTCA GTCACGATCC CGGCCGCTCG 61 AGCTATGTCT GCACATGGGA CAGCTGGATG TCGCCCCCCA CTTCCAGTCT GTTGATGGTG 121 GGCAGGTTCC TCAGGCGATG GTAGTATTCA AACAGGTGCT GACCATCCAC GGCCACCTTG 181 AGGCAGTGAG CTTCACACAA GATCCACACT GAGAAGCTCT GGCCACGGAC GAAGGGCATT 241 TTTCGGGGCA GACTTCGCTC CTCAGACCCC CAGGAGTTGT CGATCTGGGT GTTGCGGACC 301 ACAGCATTCT CATCAAAACG GGGGTTCAGG TGGAAGGCGA TGTGGTTCCC AGAGCACAGG 361 TTGATGTGGA ACCTCTGAGC ACTGGGCAGG ACAGTGCCTG ACAGGAGGAT GGACTTGGAT 421 GGGTACAGCC CTCCCAGAAT GGTGGTGATG AAAGGCATCG GATAGGCGGG GTGGGGGTAC 481 ATCATAGGTG GGATGGCGGG AGTAGAGAAC ATCTGTCCAG GGGCGCTCTG CACTGTGTGG 541 ATGACTGTCT GGGTCTGGAA GCTGATGTAG GACAGCTGCA CAGAGCCATT GACGGAGATG 601 GTGTCCACAC GGTGGAAGGG CACGCGGTGG AAGTACTGCA CGAAGAGGAT CCCGTTCACC 661 ATCACCTTGA AATCTGAGCT CTGCACCAGG AAGCAGAGGT CAAAGGGCAT CCCCTTCTGG 721 AAAGGCATGT GTGTCTTCCT CTCCTCGGGC CCCCAGCTTC CGTTCTGCCT CGTGTTGCAC 781 ACCACCGTAC CCTCCATCTT TCAAAACCGA GGGTTGAAGT GGAAGGCAAT GTCATTTCCA 841 CTGAAGCCAG TCTGAAAGTT CACAGCCAAA CCTGGTTCCA CTGGAGCTGA GAACGGTCCC 901 ATTGACAGTG ATCTGAAGTC CCGTCCTGGA GACCTCCTTG AATAAGTCCC AGAAAAGGGG 961 ACAGCTGGAC TCAAGGTAGG GAGCCTGGGA ACCGCTGAAA GGCCATGGGA ATTCCTTGGG 1021 GATCCACGGC CGGAACCAGA TCCCGAATTT TGGGAGGATG GTTCGCCAAC CAACTAAACG 1081 TTGGGCTTGC CAGCCCCTGG CAAAGGGCCA ATGCTATATT AACTTGGCTG GGAATTCCAA 1141 GGTACCTTAT CAATTGGTGG GGAATAGCCT TCCAAATTAC GGGTTATTCT TTAGAACGCC 1201 AGCAGTCCGT AGTAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol	Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl