RDB08423 - pGEX-G9NT-1a

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pGEX-G9NT-1a

Bacteria expression vector of human galectin-9 (N-terminal CRD with a part of the linker peptide, G9NT), GST fusion protein

Catalog number	RDB08423
Resource name	pGEX-G9NT-1a
Clone info.	Bacterial expression clone of an N-terminal CRD of human galectin-9 with a part of linker peptide [119 to 619nt of NM_002308.3 (cds, 119-1090)].
Comment	Expression was confirmded by the depositor by affinity purification and SDS-PAGE. Known difference :G to A substitution at 131nt (Gly to Ser) compared with NM_002308.3 , G to A substitution at 7nt compared with Cap binding site of pGEX vector (TAATGTGAGTTAGCTCACTCAT).
Vector backbone	pGEX-4T-2 (Plasmid)
Size of vector backbone	5.0 kb
Selectable markers	Amp^r
Gene/insert name	Human galectin-9 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Other clones in our bank

human galectin-9

External Database
Human galectin-9

Reference sequence

Distribution information

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Terms and conditions set forth by the DEPOSITOR	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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RDB08423	pGEX-G9NT-1a	DNA solution

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References and tips

Electronic file

Sequence	RDB08423z.seq

Original reference

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB08423_A8J9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-RSEQ Sequence file: RDB08423_A8J9a.seq
>D0521A_m2_1_A8J9_pGEX-RSEQ_A01_01_ABI24.ab1 1 TCTAATAACG GAAAAGCGCG AGGCAGGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT 61 CATGTGTGGA TGACTGTCTG GGTAATGGGA GCCGGGTTGG CAGGCCACAC GCCGGGAGGC 121 TGGAAGCTGA TGTAGGACAG CTGCACAGAG CCATTGACGG AGATGGTGTC CACACGGTGG 181 AAGGGCACGC GGTGGAAGTA CTGCACGAAG AGGATCCCGT TCACCATCAC CTTGAAATCT 241 GAGCTCTGCA CCAGGAAGCA GAGGTCAAAG GGCATCCCCT TCTGGAAAGG CATGTGTGTC 301 TTCCTCTCCT CGGGCCCCCA GCTTCCGTTC TGCCTCGTGT TGCACACCAC GTACCCTCCA 361 TCTTCAAACC GAGGGTTGAA GTGGAAGGCA ATGTCATTTC CACTGAAGCC AGTCTGAAAG 421 TTCACAGCAA ACCTGGTTCC ACTGGAGCTG AGAACGGTCC CATTGACAGT GATCTGAAGT 481 CCGTCCTGGA GACCTCCTTG AATAGTCCCA GAAAAGGGGA CAGCTGGACT CAGGTAGGGA 541 GCCTGGGAAC TGCTGAAGGC CATGGGAATT CCTGGGGATC CACGCGGAAC CAGATCCGAT 601 TTTGGAGGAT GGTCGCCACC ACCAAACGTG GCTTGCCAGC CCTGCAAAGG CCATGCTATA 661 TACTTGCTGG ATTTCAAGTA CTTATCAATT TGTGGGATAG CTTCAATACG TTTTTTAAAA 721 CAAACTAATT TTGGGAACGC ATCCAGGCAC ATTGGGTCCA TGTATAAAAC AACATCAAGA 781 GCGTCATACA ACATGAAGTC AGGATGGGTT ACATGATCAC CATTTAAATA TGTTTTATGA 841 CATAAACGAT CTTCGAACAT TTTCAGCATT TCAGGTAGCT TGCTAAGAAA ATCAACTTTG 901 AGAGTTTCAA AGTCTTTACT ATATGCAATT CTCGAAACAC CGTATCTAAT ATCCAAAACC 961 GCTCCTTCAA GCATTGAAAT CTCTGCACGG CTCTTTTGGG ACAACCACCC CAACATGTTT 1021 GTGCTTGTCA GGCTATATAA ACGGTTATGG ATGGGCCCAT AGGACTGGTG GTTTAATTTA 1081 ACATCACCCA TCAATTATAA TAAAGGAGGA ATTGGGGGAA CTCGAAGCCA AGTTCAAACC 1141 TTTTTTGGTT TCCGCCATTT ATCAACCTTT CCTATCTC //
Primer: pGEX-ptac_up Sequence file: RDB08423_A8J9b.seq
>D0521A_m2_1_A8J9_pGEX-ptac_up_F_B01_04_ABI24.ab1 1 GAAGGGCCGA CTCATAACGG TTCTGGCAAT ATTCTGAAAT GAGCTGTTGA CAATTAATCA 61 TCGGCTCGTA TAATGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGTATT 121 CATGTCCCCT ATACTAGGTT ATTGGAAAAT TAAGGGCCTT GTGCAACCCA CTCGACTTCT 181 TTTGGAATAT CTTGAAGAAA AATATGAAGA GCATTTGTAT GAGCGCGATG AAGGTGATAA 241 ATGGCGAAAC AAAAAGTTTG AATTGGGTTT GGAGTTTCCC AATCTTCCTT ATTATATTGA 301 TGGTGATGTT AAATTAACAC AGTCTATGGC CATCATACGT TATATAGCTG ACAAGCACAA 361 CATGTTGGGT GGTTGTCCAA AAGAGCGTGC AGAGATTTCA ATGCTTGAAG GAGCGGTTTT 421 GGATATTAGA TACGGTGTTT CGAGAATTGC ATATAGTAAA GACTTTGAAA CTCTCAAAGT 481 TGATTTTCTT AGCAAGCTAC CTGAAATGCT GAAAATGTTC GAAGATCGTT TATGTCATAA 541 AACATATTTA AATGGTGATC ATGTAACCCA TCCTGACTTC ATGTTGTATG ACGCTCTTGA 601 TGTTGTTTTA TACATGGACC CAATGTGCCT GGATGCGTTC CCAAAATTAG TTTGTTTTAA 661 AAAACGTATT GAAGCTATCC CACAAATTGA TAAGTACTTG AAATCCAGCA AGTATATAGC 721 ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC GACCATCCTC CAAAATCGGA 781 TCTGGTTCCG CGTGGATCCC CAGGAATTCC CATGGCCTTC AGCAGTTCCC AGGCTCCCTA 841 CCTGAGTCCA GCTGTCCCCT TTTCTGGGAC TATTCAAGGA GGTCTCCAGG ACGGACTTCA 901 GATCACTGTC AATGGGACCG TTCTCAGCTC CAGTGGAAAC CAGGGTTTGC TGTGAACTTT 961 CAGACTGGCT TCAGTGGAAA TGACATTGCC CTTCCACTTC AACCCTTCGG TTTGAAGATG 1021 GGAGGGTACG TGGTGTGCAA CACGAGGCAG ACGGAAAGCT GGGGGCCGAG GAGAGAGACA 1081 CACATGCCTT TCCAGAAGGA ATGCCTTGAC TCTGCTTCTT GATGCAGGTC TTCAGATTTT 1141 TCCAAAG //
Primer: M13_-40 Sequence file: RDB08423_A8J9c.seq
>D0521A_m2_1_A8J9_M13_-40_C01_07_ABI24.ab1 1 AAGGCAAGGG AAATCGTATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 61 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 121 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 181 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 241 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC 301 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC 361 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC 421 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC 481 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT 541 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG 601 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC 661 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC 721 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC 781 AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC 841 CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG 901 CACCGCCGCT TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC 961 ACCCAGTTGA TCGGCGCGAG ATTTAATCGC CGCGACAATT TGCGACGGCG CGTGCAGGGC 1021 CAGACTGGAG GTGGGCAACG CCCAATCAGC ACGACTGTTT GGCCCGGCCC AGGTTGGTGT 1081 GCCACGCGGG TGGGGAAATG GTAATTCAGC TTCCGTCAAT CGGCGCTCCA ACTTTTTCCC 1141 GGGCGTTTTC CGGCCAGGAA AAACCGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol	Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl