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pGEX-G9CT-1a

Bacteria expression vector of human galectin-9 (C-terminal CRD with a part of the linker peptide, G9CT), GST fusion protein

Catalog number RDB08424
Resource name pGEX-G9CT-1a
Clone info. Bacterial expression clone of a C-terminal CRD of human galectin-9 with a part of linker peptide [593 to 1090nt of NM_002308.3 (cds, 119-1090)].
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE. Known difference : C to T substitution at 676nt (Tyr to Tyr, synonym) compared with NM_002308.3
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-9 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Human galectin-9

          Reference sequence
            

          Distribution information

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          RDB08424 pGEX-G9CT-1a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08424z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08424_A8J9p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-RSEQ
          Sequence file: RDB08424_A8J9a.seq check
          >D0521B_m1_1_A8J9_pGEX-RSEQ_D01_10_ABI24.ab1
              1 TCTACTAACC GGAAACGGCG CGGAGGCAGA TCGTCAGTCA GTCACGATGC GGCCGCTCGA
             61 GCTATGTCTG CACATGGGTC AGCTGGATGT CGCCCCCCAC TTCCAGTCTG TTGATGGTGG
            121 GCAGGTTCCT CAGGCGATGG TAGTATTCAA ACAGGTGCTG ACCATCCACG GCCACCTTGA
            181 GGCAGTGAGC TTCACACAAG ATCCACACTG AGAAGCTCTG GCCACGGACG AAGGGCATTT
            241 TTCGGGGCAG ACTTCGCTCC TCAGACCCCC AGGAGTTGTC GATCTGGGTG TTGCGGACCA
            301 CAGCATTCTC ATCAAAACGG GGGTTCAGGT GGAAGGCGAT GTGGTTCCCA GAGCACAGGT
            361 TGATGTGGAA CCTCTGAGCA CTGGGCAGGA CAGTGCCTGA CAGGAGGATG GACTTGGATG
            421 GGTACAGCCC TCCCAGAATG GTGGTGATGA AAGGCATCGG ATAGGCGGGG TGGGGATACA
            481 TCATAGGTGG GATGGCGGGA GTAGAGAACA TCTGTCCAGG GGCGCTCTGC ACTGTGTGGA
            541 TGACTGTCTG GGTAATGGGA ATTCCTGGGG ATCCACGCGG AACCAGATCC GATTTTGGAG
            601 GATGGTCGCC ACCACCAAAC GTGGCTTGCC AGCCCTGCAA AGGCCATGCT ATATACTTGC
            661 TGGATTTCAA GTACTTATCA ATTTGTGGGA TAGCTTCAAT ACGTTTTTTA AAACAAACTA
            721 ATTTTGGGAA CGCATCCAGG CACATTGGGT CCATGTATAA AACAACATCA AGAGCGTCAT
            781 ACAACATGAA GTCAGGATGG GTTACATGAT CACCATTTAA ATATGTTTTA TGACATAAAC
            841 GATCTTCGAA CATTTTCAGC ATTTCAGGTA GCTTGCTAAG AAAATCAACT TTGAGAGTTT
            901 CAAAGTCTTT ACTATATGCA ATTCTCGAAA CACCGTATCT AATATCCAAA ACCGCTCCTT
            961 CAAGCATTGA AATCTCTGCA CGCTCTTTTG GACAACCACC CAACATGTTG TGCTTGTCAG
           1021 CTATATAACG TATGATGGGC CATAGACTGG TGTTAATTTT ACATCACCAT CAATATAATA
           1081 AAGAAGATTG GGGAAACTCC AAACCAATCA ACTTTTGGTT CGCAATTTAA TCACCTTCCA
          1141 TCGCGCGCCT CATACAAAAA TTGCGCTCCT
          //
          Primer: pGEX-ptac_up
          Sequence file: RDB08424_A8J9b.seq check
          >D0521B_m1_1_A8J9_pGEX-ptac_up_F_E01_13_ABI24.ab1
              1 AATTCCGCGG CCATCATAAC GGGTTCTGGC AAATATTCTG AAATGAGCTG TTGACAATTA
             61 ATCATCGGCT CGTATAATGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
            121 TATTCATGTC CCCTATACTA GGTTATTGGA AAATTAAGGG CCTTGTGCAA CCCACTCGAC
            181 TTCTTTTGGA ATATCTTGAA GAAAAATATG AAGAGCATTT GTATGAGCGC GATGAAGGTG
            241 ATAAATGGCG AAACAAAAAG TTTGAATTGG GTTTGGAGTT TCCCAATCTT CCTTATTATA
            301 TTGATGGTGA TGTTAAATTA ACACAGTCTA TGGCCATCAT ACGTTATATA GCTGACAAGC
            361 ACAACATGTT GGGTGGTTGT CCAAAAGAGC GTGCAGAGAT TTCAATGCTT GAAGGAGCGG
            421 TTTTGGATAT TAGATACGGT GTTTCGAGAA TTGCATATAG TAAAGACTTT GAAACTCTCA
            481 AAGTTGATTT TCTTAGCAAG CTACCTGAAA TGCTGAAAAT GTTCGAAGAT CGTTTATGTC
            541 ATAAAACATA TTTAAATGGT GATCATGTAA CCCATCCTGA CTTCATGTTG TATGACGCTC
            601 TTGATGTTGT TTTATACATG GACCCAATGT GCCTGGATGC GTTCCCAAAA TTAGTTTGTT
            661 TTAAAAAACG TATTGAAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC AGCAAGTATA
            721 TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT CCTCCAAAAT
            781 CGGATCTGGT TCCGCGTGGA TCCCCAGGAA TTCCCATTAC CCAGACAGTC ATCCACACAG
            841 TGCAGAGCGC CCCTGGACAG ATGTTCTCTA CTCCCGCCAT CCCACCTATG ATGTATCCCC
            901 ACCCCGCCTA TCCGATGCCT TTCATCACCA CCATTCTGGG AGGGGCTGTA CCCATCCAAG
            961 TCCATCCCTC CTGTCAGGCA CTGGTCCTGC CCAGTGCTCA GAGGGTTCCA CATCAACCCT
           1021 GGTGCTCTGG GAAACACATC GGCCTTTCCA CCTGAACCCC CCGTTTTGAA TGAAGAATGC
           1081 TGTGGTCGCC AACACCAGGA TCGACAACTC CTTGGGGATC TGAGGAGCGA AGTCTTGCCT
          1141 CCGGAAAAAT GTCCCCTTCG AGTTCCCGTT GAGTCA
          //
          Primer: M13_-40
          Sequence file: RDB08424_A8J9c.seq check
          >D0521B_m1_1_A8J9_M13_-40_F01_16_ABI24.ab1
              1 AGCCAAGGGG AATCCGTATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
             61 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
            121 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
            181 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
            241 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC
            301 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC
            361 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC
            421 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC
            481 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT
            541 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG
            601 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC
            661 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC
            721 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGATGG GTGTCTGGTC
            781 AGAGACATCA AGAAATAACG CCGGAACATT AGTGCAGGCA GCTTCCACAG CAATGGCATC
            841 CTGGTCATCC AGCGGATAGT TAATGATCAG CCCACTGACG CGTTGCGCGA GAAGATTGTG
            901 CACCGCCGCT TTACAGGCTT CGACGCCGCT TCGTTCTACC ATCGACACCA CCACGCTGGC
            961 ACCCAGTTGA TCGGGCGCGA GATTTAATCG CCGCGACAAT TTGCGACGGC GCGGTGCAGG
           1021 GGCCAGACTG GGAGGTGGCA ACGCCCAATC AGCAAACGAC TGTTGCCCGG CCAAGTTTGT
           1081 GTTGCCACGC GTTGGGGAAT GTAATCAGCT TCCGCCATCG CCGGCTTCCA CTTTTCCCCG
          1141 CCCGTTTTTC GCCCAGGAAA AACGCGGTG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl