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pGEX-G3-1a

Bacteria expression vector of human galectin-3, GST fusion protein

Catalog number RDB04219
Resource name pGEX-G3-1a
Clone info. Expression vector of human galectin-3.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-3 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank
External Database
Human galectin-3
          Reference sequence
            

          Distribution information

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          RDB04219 pGEX-G3-1a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB04219.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13382_A5Ft.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F
          Sequence file: RDB13382_A5Fta.seq check
          >04219_13382_A5Ft_A_pGEX-F_A01_01.ab1
              1 GGCATCGTCA ATCGGATCTG GTTCCGCGTG GATCCCCAGG AATTCCCATG GCAGACAATT
             61 TTTCGCTCCA TGATGCGTTA TCTGGGTCTG GAAACCCAAA CCCTCAAGGA TGGCCTGGCG
            121 CATGGGGGAA CCAGCCTGCT GGGGCAGGGG GCTACCCAGG GGCTTCCTAT CCTGGGGCCT
            181 ACCCCGGGCA GGCACCCCCA GGGGCTTATC CTGGACAGGC ACCTCCAGGC GCCTACCATG
            241 GAGCACCTGG AGCTTATCCC GGAGCACCTG CACCTGGAGT CTACCCAGGG CCACCCAGCG
            301 GCCCTGGGGC CTACCCATCT TCTGGACAGC CAAGTGCCCC CGGAGCCTAC CCTGCCACTG
            361 GCCCCTATGG CGCCCCTGCT GGGCCACTGA TTGTGCCTTA TAACCTGCCT TTGCCTGGGG
            421 GAGTGGTGCC TCGCATGCTG ATAACAATTC TGGGCACGGT GAAGCCCAAT GCAAACAGAA
            481 TTGCTTTAGA TTTCCAAAGA GGGAATGATG TTGCCTTCCA CTTTAACCCA CGCTTCAATG
            541 AGAACAACAG GAGAGTCATT GTTTGCAATA CAAAGCTGGA TAATAACTGG GGAAGGGAAG
            601 AAAGACAGTC GGTTTTCCCA TTTGAAAGTG GGAAACCATT CAAAATACAA GTACTGGTTG
            661 AACCTGACCA CTTCAAGGTT GCAGTGAATG ATGCTCACTT GTTGCAGTAC AATCATCGGG
            721 TTAAAAAACT CAATGAAATC AGCAAACTGG GAATTTCTGG TGACATAGAC CTCACCAGTG
            781 CTTCATATAC CATGATATAA CTCGAGCGGC CGCATCGTGA CTGACTGACG ATCTGCCTCG
            841 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG
            901 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG
            961 GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGGAGT GTATAATTCT
           1021 TGAAGACGAA AGGCCTCGTG ATACGCCTAT TTTTTTATAG GTTATGTCAT GATAATAATG
           1081 GACTTTTCTT AGACGGTCAA GCTGCACTTT TCTGGCAATG TGCGCGGCAA TCCTACTGAT
          1141 TAATTTTTCG TAAAT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl