Bacteria expression vector of human galectin-4, GST fusion protein
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External Database Human galectin-4 Reference sequence |
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Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB08396 | pGEX-G4-1a | DNA solution |
Electronic file
Sequence | RDB08396z.seq |
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB08396_A9Hsp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: GST-M_F Region: GST 3', thrombin site, insert5' Sequence file: RDB08396_A9Hsa.seq |
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>08396_08396_A9Hs_1_GST-M_F_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NCNNNNNTTG NNTTTCTTAG CAAGCTACCT GAAATGCTGA 61 AAATGTTCGA AGATCGTTTA TGTCATAAAA CATATTTAAA TGGTGATCAT GTAACCCATC 121 CTGACTTCAT GTTGTATGAC GCTCTTGATG TTGTTTTATA CATGGACCCA ATGTGCCTGG 181 ATGCGTTCCC AAAATTAGTT TGTTTTAAAA AACGTATTGA AGCTATCCCA CAAATTGATA 241 AGTACTTGAA ATCCAGCAAG TATATAGCAT GGCCTTTGCA GGGCTGGCAA GCCACGTTTG 301 GTGGTGGCGA CCATCCTCCA AAATCGGATC TGGTTCCGCG TGGATCCCCA GGAATTCCCA 361 TGGCCTATGT CCCCGCACCG GGCTACCAGC CCACCTACAA CCCGACGCTG CCTTACTACC 421 AGCCCATCCC GGGCGGGCTC AACGTGGGAA TGTCTGTTTA CATCCAAGGA GTGGCCAGCG 481 AGCACATGAA GCGGTTCTTC GTGAACTTTG TGGTTGGGCA GGATCCGGGC TCAGACGTCG 541 CCTTCCACTT CAATCCGCGG TTTGACGGCT GGGACAAGGT GGTCTTCAAC ACGTTGCAGG 601 GCGGGAAGTG GGGCAGCGAG GAGAGGAAGA GGAGCATGCC CTTCAAAAAG GGTGCCGCCT 661 TTGAGCTGGT CTTCATAGTC CTGGCTGAGC ACTACAAGGT GGTGGTAAAT GGAAATCCCT 721 TCTATGAGTA CGGGCACCGG CTTCCCCTAC AGATGGTCAC CCACCTGCAA GTGGATGGGG 781 ATCTGCAACT TCAATCAATC AACTTCATCG GANGCCAGCC CCTCCGGCCC CAGGNNCCCC 841 CGATGATGCC ACCTTACCCT NGTCCCGGAC ATTGCCATCA ACAGCTGAAC AGCCTGCCNA 901 CCATGGANNG // |
Primer: pJB8-Forward Region: insert3' Sequence file: RDB08396_A9Hsb.seq |
>08396_08396_A9Hs_1_JB8-Forward_E07_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTG GGTNNNGGCT GCGCCCCGAC ACCCGCCNAC ACCCGCTGAC 61 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC 121 GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGATCGTC 181 AGTCAGTCAC GATGCGGCCG CTCGAGTTAG ATCTGGACAT AGGACAAGGT GACATCACCC 241 TGGATTTCCA ATGTGTCCAC CCTCTGGAAG GCCGAGAGGC GATGGGCAAA GTCAAAGAGG 301 TGCTGGCCAT TGGCGTAAAC CTTGAAGCGA TCCAAGCCAC AGCGAATGGA CAGATCAAAG 361 AACTGTCCGG GACCAAATGG GTTGTGGGTG ATCTTCTTCT CCTCGGATCC CCACGAGCCA 421 TTCAGAAGGC TGTTCCGGAC CACGGTACCG TTGCCCATGC GGGGATTAAT GTGCAGAGCT 481 ATGTCCCCTG AGGAGCCCAC CTTGAAGTTG ATAGCAAAGC TCTTGCCTGT GGGAGGCACA 541 TAGCCCTTGA TGATGATGGT TCTTCGAGCT GTGAGCCCTC CTTGCAGCCT CCCGAAATAT 601 GGCACAGGCG GGTTGAAGGT TGGGGGTCCT TCCATGGTGG GCANG // |
Primer: pUC-M13_-100 Region: lacZ alpha, lacO, lacI gene Sequence file: RDB08396_A9Hsc.seq |
>08396_08396_A9Hs_1_pUC-M13_-100_F07_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNNNGCNNG GNNTTTCCNN GTCACGACGT TGTAAAACGA 61 CGGCCAGTGA ATCCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 121 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 181 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 241 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 301 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC 361 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC 421 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC 481 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC 541 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT 601 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG 661 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC 721 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC 781 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAATA CTGTTGANGG GTGTCTGGTC 841 ANAGANATCA AGAAATAACG CCGGANNNNT ANTGCAGGCA GCTTCCACAG CAA // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
protocol | Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). |
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2022.05.17
GNP_filter3_RDBDEP_html_220516.pl