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pGEX-mG8(M)-2a

Bacteria expression vector of mouse galectin-8 (the predominant isoform of mG8 in most cell and tissue types, mG8(M)/mG8M), GST fusion protein

Catalog number RDB08440
Resource name pGEX-mG8(M)-2a
Clone info. Bacterial expression clone of mouse galectin-8.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse galectin-8 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Mouse galectin-8

          Reference sequence
            

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
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          RDB08440 pGEX-mG8(M)-2a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08440z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08440_A2Cnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST, thrombin site, insert 5'
          Sequence file: RDB08440_A2Cna.seq check
          >08440_08440_B1HbF_pGEX-F2_A04_01_ABI08.ab1
              1 NNNNNNNNNN NNNTNNNNNN NGTANTNNNC TATCCCNCAA ATTGATAAGT ACTTGAAATC
             61 CAGCAAGTAT ATAGCATGGC CTTTGCAGGG CTGGCAAGCC ACGTTTGGTG GTGGCGACCA
            121 TCCTCCAAAA TCGGATCTGG TTCCGCGTGG ATCCATGTTG TCCTTAAATA ACCTACAAAA
            181 TATCATCTAT AACCCGATAA TCCCCTATGT TGGCACCATT ACTGAGCAAT TGAAGCCTGG
            241 CTCTCTGATT GTAATCCGTG GGCATGTCCC TAAAGATTCA GAAAGATTCC AGGTTGACTT
            301 TCAGCTGGGC AACAGCCTGA AGCCAAGAGC AGACGTGGCC TTCCACTTTA ACCCTCGGTT
            361 CAAAAGGTCT AGCTGCATTG TTTGTAACAC ACTGACACAG GAGAAGTGGG GCTGGGAGGA
            421 GATCACCTAC GACATGCCCT TCAGAAAAGA AAAGTCCTTT GAGATCGTGT TCATGGTGCT
            481 CAAGAACAAA TTCCAGGTGG CTGTGAACGG AAGGCATGTT CTGCTGTACG CCCACAGGAT
            541 CAGCCCGGAG CAGATCGACA CAGTGGGCAT CTACGGCAAA GTGAACATCC ACTCCATCGG
            601 GTTCAGATTC AGCTCGGATT TACAGAGTAT GGAAACATCT GCTCTGGGAC TGACACAGAT
            661 AAACAGAGAG AATATACAAA AGCCAGGCAA GCTCCAGCTG AGCCTGCCAT TTGAAGCAAG
            721 GTTGAATGCC TCCATGGGTC CTGGACGAAC CGTTGTCATT AAAGGGGAAG TGAACACCAA
            781 TGCCCGAAGC TTTAATGTTG ACCTANTGGC ANGAAAAACA AGGGATATCG CTCTGCANTT
            841 NAACCNACGC CTCAANGTGA AAGCATTTGT AAGAAANTCC TTTNCTTCNN NNTGNCNTGG
            901 GGAGAANANG ANANAAATAT TACCNGCTTN CNNTTTANNT CTGGNNNGNN NTTTGANNTG
          961 ANNNNCNANN NNNNNGNCNN NNNNNNNN
          //
          Primer: pJB8-Forward
          Region: insert 3'
          Sequence file: RDB08440_A2Cnb.seq check
          >08440_08440_B1HbF_pJB8-Forward_B04_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN TGGGTNNNGG CTGCGCCCCG ACNNCCCGCC AACACCCGCT
             61 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC
            121 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGATC
            181 GTCAGTCAGT CACGATGCGG CCGCTCGAGC TACCAGCTCC TTACATCCAG CAAACGGATA
            241 TCACCATCGA CTGATAGTGT ATCAATACTG CTTAGGTCTT TAAATCTGTG TTTGTACTCC
            301 AGGCTGTGCA CACCATTTAT AGCAACCTTG AATTCCCGGA CATCACAGTA GATTATCATC
            361 TCAAAGTACA TCCCAGAACT AAATGGGAAG CAGGTAATAT TTCTCTCCTC TTCTCCCCAG
            421 GCATCCTGAA GAAAGGAATT TCTTACAAAT GCTTTCACAT TGAGGCGTGG GTTCAAGTGC
            481 AGAGCGATAT CCCTTGTTTT TCCTGCCACT AGGTCAACAT TAAAGCTTCG GGCATTGGTG
            541 TTCACTTCCC CTTTAATGAC AACGGTTCGT CCAGGACCCA TGGAGGCATT CAACCTTGCT
            601 TCAAATGGCA GGCTCAGCTG GAGCTTGCCT GGCTTTTGTA TATTCTCTCT GTTTATCTGT
            661 GTCAGTCCCA GAGCAGATGT TTCCATACTC TGTAAATCCG AGCTGAATCT GAACCCGATG
            721 GAGTGGATGT TCACTTTGCC GTAGATGCCC ACTGTGTCGA TCTGCTCCGG GCTGATCCTG
            781 TGGGCGTACA GCAGAACATG CCTTCCGTTC ACAGCCACCT GGAATTTGTT CTTGANCACC
            841 ATGAACACGA TCTCAAAGGA NTTTTCTTTT CTGAAGGGCA TGTCGNANNT GATCTCCNCC
            901 CNNCCCCNCT TCTCCTGNGN CAGNGNGTTA CNAANNNANN GNANCNNNNC NTTTTNNANC
          961 CNANGGNTNN NNNNNNNN
          //
          Primer: lacIpro-B_F
          Region: lacI pro, lacI
          Sequence file: RDB08440_A2Cnc.seq check
          >08440_08440_B1HbF_lacIpro-B_F_C04_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNGC NNCGNNTCNG TGAGCGAGGA ANCGGAAGAN CGCCTGATGC
             61 GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAAATTCC GACACCATCG
            121 AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG
            181 TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC
            241 AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG
            301 TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG
            361 GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC
            421 AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA
            481 TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC
            541 GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG
            601 CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA
            661 GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG
            721 GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC
            781 TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCNAATTCA GCCGATAGCG GAACGGNAAN
            841 GCGACTGGAG TGNCATGTCC GGTTTTCNAC AAANCATGCA ANTGCTGAAT GANGGCATCG
            901 TTCCCNCTGC GANGNNGGTN GCCAACGATC NNANGGNGCT GGGCGCNNNG CGCGCCATTA
          961 CNNNNNNCNN NNTGNNNNNN NGNTGNGNNN ATNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl