Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-mG3-1a

Bacteria expression vector of mouse galectin-3, GST fusion protein

Catalog number RDB08436
Resource name pGEX-mG3-1a
Alternative name pGEx-mG3-3a
Clone info. Bacterial expression clone of mouse galectin-3.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Mouse galectin-3 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Mouse galectin-3

          Reference sequence
            X16834.1
           
          Sequence (full) RDB08436zzt01.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースの利用は、学術機関の学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08436 pGEX-mG3-1a DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19444_B1Kqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST, thrombin site, insert 5'
          Sequence file: RDB19444_B1Kqa.seq check
          >D08453A7_B1Kq_1_pGEX-F2_A10_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNTNN NNNNNNTATN NNNNNTATCC CNCAAATTGA TAAGTACTTG
             61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC
            121 GACCATCCTC CAAAATCGGA TCTGGTTCCG CGTGGATCCA TGGCAGACAG CTTTTCGCTT
            181 AACGATGCCT TAGCTGGCTC TGGAAACCCA AACCCTCAAG GATATCCGGG TGCATGGGGG
            241 AACCAGCCTG GGGCAGGGGG CTACCCAGGG GCTGCCTATC CTGGGGCCTA TCCAGGACAG
            301 GCTCCTCCAG GGGCCTACCC AGGACAGGCT CCTCCAGGGG CCTATCCAGG ACAGGCTCCT
            361 CCTAGTGCCT ACCCCGGCCC AACTGCCCCT GGAGCTTATC CTGGCCCAAC TGCCCCTGGA
            421 GCTTATCCTG GCTCAACTGC CCCTGGAGCC TTCCCAGGGC AACCTGGGGC ACCTGGGGCC
            481 TACCCCAGTG CTCCTGGAGG CTATCCTGCT GCTGGCCCTT ATGGTGTCCC CGCTGGACCA
            541 CTGACGGTGC CCTATGACCT GCCCTTGCCT GGAGGAGTCA TGCCCCGCAT GCTGATCACA
            601 ATCATGGGCA CAGTGAAACC CAACGCAAAC AGGATTGTTC TAGATTTCAG GAGAGGGAAT
            661 GATGTTGCCT TCCACTTTAA CCCCCGCTTC AATGAGAACA ACAGAAGAGT CATTGTGTGT
            721 AACACGAAGC AGGACAATAA CTGGGGAAAG GAAGAAAGAC AGTCANCCTT CCCCTTTGAG
            781 AGTGNAAAAC CATTCNAAAT ACAAGTCCTG GTTGAAGCTG ANCACTTCNA GGTTGCGGTC
            841 AACGATGCTC ACCTACTGCA GTACAACCAT CGGATGAAGA ANCTCCGGGA AATCANNCNA
            901 CTGGGGATCA GTGGNGNNAT NNNCCTCACN ANCGCTANNC ACGCCNTGAT CTNAGAATTC
            961 CCGGGNCGAN TCNANCNGNN NGCATCGNGA NNGANNGANN NNCNNNCTCN NNNNNTNNNN
          1021 NGANGANNNN NAAAANCNNN NNNNNNN
          //
          Primer: pJB8-Forward
          Region: insert 3'
          Sequence file: RDB19444_B1Kqb.seq check
          >D08453A7_B1Kq_1_pJB8-Forward_B10_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGTN NNGNNCTGCG CCCCGACACC CGNNNNNNCC
             61 CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC
            121 CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA
            181 GATCGTCAGT CAGTCACGAT GCGGCCGCTC GAGTCGACCC GGGAATTCTT AGATCATGGC
            241 GTGGTTAGCG CTGGTGAGGG TTATGTCACC ACTGATCCCC AGTTGGCTGA TTTCCCGGAG
            301 GTTCTTCATC CGATGGTTGT ACTGCAGTAG GTGAGCATCG TTGACCGCAA CCTTGAAGTG
            361 GTCAGCTTCA ACCAGGACTT GTATTTTGAA TGGTTTTCCA CTCTCAAAGG GGAAGGCTGA
            421 CTGTCTTTCT TCCTTTCCCC AGTTATTGTC CTGCTTCGTG TTACACACAA TGACTCTTCT
            481 GTTGTTCTCA TTGAAGCGGG GGTTAAAGTG GAAGGCAACA TCATTCCCTC TCCTGAAATC
            541 TAGAACAATC CTGTTTGCGT TGGGTTTCAC TGTGCCCATG ATTGTGATCA GCATGCGGGG
            601 CATGACTCCT CCAGGCAAGG GCAGGTCATA GGGCACCGTC AGTGGTCCAG CGGGGACACC
            661 ATAAGGGCCA GCAGCAGGAT AGCCTCCAGG AGCACTGGGG TANGCCCCAG GTGCCCCAGG
            721 TTGNCCTGGG AANGCTCCAG GGGCAGTTGA GCCAGGATAA GCTCCNGGGG CAGTTGGGCC
            781 AGGATAAGCT CCAGGGGCAG TTGGGCCNGG GTAGGCACTA NGANGANCCT GTCCNGNATA
            841 NNNCCCTGGA NGANCCTGTC CTGGGTAGGC CCCTGGANGA NCCTGTNCTG NANANGNCCC
            901 ANGATANGNA GCCCCNNNNN NANNCCCCCT NCCCCAGGCT GGTTCCCCCA TGNACCCNNN
            961 NATNCNTTGA NGGNTNGGGN NTTCNNNANC CNGCTNNNNN NCGNTNNANC NNAAAGCTGN
          1021 NTNNCNNNNN NNNNN
          //
          Primer: lacIpro-B_F
          Region: lacI pro, lacI
          Sequence file: RDB19444_B1Kqc.seq check
          >D08453A7_B1Kq_1_lacIpro-B_F_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNC ANCGNNTCNG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC
             61 GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAAATTCC GACACCATCG
            121 AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG
            181 TGGTGAATGT GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC
            241 AGACCGTTTC CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG
            301 TGGAAGCGGC GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG
            361 GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC
            421 AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA
            481 TGGTAGAACG AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC
            541 GCGTCAGTGG GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG
            601 CTGCCTGCAC TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA
            661 GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG
            721 GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC
            781 TGGCTGGCTG GCATAAATAT CTCACTCGCA ATCNAATTCA GCCGATAGCG GAACGGGAAG
            841 GCGANTGGAG TGCCATGTCC GGNTTTCAAC NAACCATGCA AATGCTGAAT GANGGCATCG
            901 TTCCCNCTGC GATGCTGGTT GNCNACGATC AGATGGNGCT GGGNGCAATG CGCGCNNTTA
            961 CNNANNCCNG GCNGNNCGTT GGNGNGNATN TNNCGGNANT GGGANNNNNN NANNNNNNNN
          1021 NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.08.12

          GNP_filter3_RDBDEP_html_220812.pl