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pGEX-G9(M)-1a

Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M), GST fusion protein

Catalog number RDB04223
Resource name pGEX-G9(M)-1a
Clone info. Expression vector of galectin-9.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 9 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Human lectin, galactoside-binding, soluble, 9

          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB04223.pdf

          Distribution information

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          RDB04223 pGEX-G9(M)-1a DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Matsushita, N., Requirement of divalent galactoside-binding activity of ecalectin/galectin-9 for eosinophil chemoattraction. J. Biol. Chem., 275, 8355-8360 (2000). PMID 10722666.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19857_B2Lpp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: GST-M_F (Pr0153)
          Region: GST 3',thrombin site,insert 5'
          Sequence file: RDB19857_B2Lpa.seq check
          >04223_19857_B2Lp_gs3_GST-M_F_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNANN NNNNNNNNNN TNNNNTTTNN NNGNNNNNNN CCTGNAAATG
             61 CTGAAAATGG TTCGAAGATC NNTTTATGTC ATAAAACATA TTTAAATGGT GATCATGTAA
            121 CCCATCCTGA CTTCATGTTG TATGACGCTC TTGATGTTGT TTTATACATG GACCCAATGT
            181 GCCTGGATGC GTTCCCAAAA TTAGTTTGTT TTAAAAAACG TATTGAAGCT ATCCCACAAA
            241 TTGATAAGTA CTTGAAATCC AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA
            301 CGTTTGGTGG TGGCGACCAT CCTCCAAAAT CGGATCTGGT TCCGCGTGGA TCCCCAGGAA
            361 TTCCCATGGC CTTCAGCGGT TCCCAGGCTC CCTACCTGAG TCCAGCTGTC CCCTTTTCTG
            421 GGACTATTCA AGGAGGTCTC CAGGACGGAC TTCAGATCAC TGTCAATGGG ACCGTTCTCA
            481 GCTCCAGTGG AACNNNGTTT GCTGTGAACT TTCAGACTGG CTTCAGTGGA AATGACATTG
            541 CCTTCCACTT CAACCCTCGG TTTGAAGATG GAGGGTACGT GGTGTGCANN NNNAGGCAGA
            601 ACGGAAGCTG GGGGCCCGAG GAGAGGAAGA CACACATGCC TTTCCANAAN GGGATGCCCT
            661 TTGACCTCTN NTNNNTGGTG CAGAGCNNNN ATTTCAAGGT GATGGTGNNN NNGATCCTCT
            721 TCGTGCANNN NNNNCACCGC GTGCCCTTNC ACCGTGTGGA CACCATCNCN NNTCNATGGC
          781 TCTGTGCANN TGNCNNNNNT CANCTNCNNN NNNNNNN
          //
          Primer: pJB8-Forward (Pr0084)
          Region: insert 3'
          Sequence file: RDB19857_B2Lpb.seq check
          >04223_19857_B2Lp_gs3_pJB8-Forward_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGCTGCN CCCCNNACNC CCNNNCNNNN
             61 NCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG
            121 ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG
            181 CAGATCGTCA GTCAGTCACG ATGCGGCCGC TCGAGCTATG TCTGCACATG GGTCAGCTGG
            241 ATGTCGCCCC CCACTTCCAG TCTGTTGATG GTGGGCAGGT TCCTCAGGCG ATGGTAGTAT
            301 TCAAACAGGT GCTGACCATC CACGGCCACC TTGAGGCAGT GAGCTTCACA CAAGATCCAC
            361 ACTGAGAAGC TCTGGCCACG GACGAAGGGC ATTTTTCGGG GCAGACTTCG CTCCTCAGAC
            421 CCCCAGGAGT TGTCGATCTG GGTGTTGCGG ACCACAGCAT TCTCATCAAA ACGGGGGTTC
            481 AGGTGGAAGG CGATGTGGTT CCCAGAGCAC AGGTTGATGT GGAACCTCTG AGCACTGGGC
            541 AGGACAGTGC CTGACAGGAG GATGGACTTG GATGGGTACA GCCCTCCCAG AATGGTGGNN
            601 NNNNAAGGCA TCGGNNNNNN GGGGTGGGGG NANNTNATAG GTGGGATGGC GGGAGTAGAG
            661 AACATCTGTN NNNGGGCGCT CTGCACTGTG TGGATGACTG TCTGGGNNNN GNGAGCCGGG
            721 TTGGCAGGNC ACACGCCGGG AGGCTGGAAG CTGATGTANG ACANNNNNNC ANANNNNTTG
            781 ACGGANANGG NGTCCACACG GTGGAAGGGC ACGCNNTGGA ANTACTGCAC NNAAGAGGAT
            841 NCCCGTTNCA CCATNNNNNT NAAATCTGAN CTCTGCNCCA NNNANCNNAN GTNNAANNGN
            901 CNTNCNNNTN NGGANNNGNN NNNNNNGTCN NNNNNCTCCT CNNNNCCCCN NNNTNNNNTT
            961 NNNNNNTCGN NNTGNNNNNC NNNNNNCNNN CNNTNNNNNN AACNNNNNGG NTGAAGNNNN
          1021 ANGNNNNNNN NTTNCNNNNN NNNNN
          //
          Primer: pGEX_F (Pr0076)
          Region: insert 5'
          Sequence file: RDB19857_B2Lpc.seq check
          >04223_19857_B2Lp_gs3_pGEX_F_H02_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN NNNNCGTGGN NCCCCNNGAA TTCCCATGGC
             61 CTTCAGCGGT TCCCAGGCTC CCTACCTGAG TCCAGCTGTC CCCTTTTCTG GGACTATTCA
            121 AGGAGGTCTC CAGGACGGAC TTCAGATCAC TGTCAATGGG ACCGTTCTCA GCTCCAGTGG
            181 AACCAGGTTT GCTGTGAACT TTCAGACTGG CTTCAGTGGA AATGACATTG CCTTCCACTT
            241 CAACCCTCGG TTTGAAGATG GAGGGTACGT GGTGTGCAAC ACGAGGCAGA ACGGAAGCTG
            301 GGGGCCCGAG GAGAGGAAGA CACACATGCC TTTCCAGAAG GGGATGCCCT TTGACCTCTG
            361 CTTCCTGGTG CAGAGCTCAG ATTTCAAGGT GATGGTGAAC GGGATCCTCT TCGTGCAGTA
            421 CTTCCACCGC GTGCCCTTCC ACCGTGTGGA CACCATCTCC GTCAATGGCT CTGTGCAGCT
            481 GTCCTACATC AGCTTCCAGC CTCCCGGCGT GTGGCCTGCC AACCCGGCTC CCATTACCCA
            541 GACAGTCATC CACACAGTGC AGAGCGCCCC TGGACAGATG TTCTCTACTC CCGCCATCCC
            601 ACCTATGATG TACCCCCACC CCGCCTATCC GATGCCTTTC ATCACCACCA TTCTGGGAGG
            661 GCTGTACCCA TCCAAGTCCA TCCTCCTGTC AGGCACTGTC CTGCCCAGTG CTCANAGGTT
            721 CCACATCAAC CTGTGCTCTG GGAACCACAT CGCCTTCCAC CTGAACCCCC GTTTTGATGA
            781 NAATGCNNNN NNCCNCAACA CCCAGATCNA CAACTCCTGG GGGTCTGANG AGCGAAGTCT
            841 GCCCNNAAAA ATGCCCTTCN TCCGTGGNCA GANCTTCTCN NNGTGGATCT TGTGTGAANC
            901 TCANTGNCTC ANNNTGNNCG NNGATGGNNC NGCACCTGTT TGAANNCTAC NATCNNCCTG
            961 NNGNAANCNT GCCCANNATC ANCNNANNNG NNAGNNGGGG GGNNNANNTC NNGCNNNNCC
           1021 NTGNNCANAN NNANCNNGAN CNGNNNNCNN CNNNNANTGN NNNNNNNTNT NNCNNNNNNN
          1081 NNTNNNNNNN NGANNNNNAA NCNNNNNNNN NN
          //
          Primer: pUC-M13_-100 (Pr0310)
          Region: lacZ alpha,lacO,lac pro,lacI
          Sequence file: RDB19857_B2Lpd.seq check
          >04223_19857_B2Lp_gs3_pUC-M13_-100_B02_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGTT TTCCCNGTCA CGACGTTGTA
             61 AAACGACGGC CAGTGAATCC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
            121 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
            181 TAATGAGTGA GCTAACTTAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
            241 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
            301 ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT
            361 CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG
            421 AAAATCCTGT TTGATGGTGG TTAACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC
            481 GTATCCCACT ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT
            541 TGCGCCCAGC GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT
            601 CAGCATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC
            661 TATCGGCTGA ATTTGATTGC NAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC
            721 CGAGACAGAA CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG
            781 ATGCTCCACG CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGGTG
            841 TCTGGNCAGA GACATCNAAG AAATAACNNC NGNANCATTA GTGNNNGGCA NCTTCCACAN
            901 CAATGGNNNN NNTGGTCATC NNNCGGATAG TTNANNNTCA GCCCACTNAN NCNNTNNCGC
            961 GAGAANNNTG TGCANCNCCG CTTTACNNGC NTNNNACNNN GCNTNNGTTC TANCNTCNNN
           1021 NNNCNNNNAC NNNTGGCNNN NNNNTNNATN NGNNCNNGAN TNNANNCNNC NGCGNNNANT
          1081 TNNNNNNNNG NNNNNNNGCN NGNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsushita, N., Requirement of divalent galactoside-binding activity of ecalectin/galectin-9 for eosinophil chemoattraction. J. Biol. Chem., 275, 8355-8360 (2000). PMID 10722666.
          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2023.01.18

          GNP_filter3_RDBDEP_html_221125.pl