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pGEX-G9(M)-1a

Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M), GST fusion protein

Catalog number RDB04223
Resource name pGEX-G9(M)-1a
Clone info. Expression vector of galectin-9.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 9 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Human lectin, galactoside-binding, soluble, 9

          Reference sequence
            

          Distribution information

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          RDB04223 pGEX-G9(M)-1a DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB04223.pdf

          Original reference

          original Matsushita, N., Requirement of divalent galactoside-binding activity of ecalectin/galectin-9 for eosinophil chemoattraction. J. Biol. Chem., 275, 8355-8360 (2000). PMID 10722666.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13756_A6A4p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F
          Sequence file: RDB13756_A6A4a.seq check
          >04223_04223_A6A4_3_pGEX-F_C12_09.ab1
              1 TGCATCATCA AATCGGATCT GGTTCCGCGT GGATCCCCAG GAATTCCCAT GGCCTTCAGC
             61 AGTTCCCAGG CTCCCTACCT GAGTCCAGCT GTCCCCTTTT CTGGGACTAT TCAAGGAGGT
            121 CTCCAGGACG GACTTCAGAT CACTGTCAAT GGGACCGTTC TCAGCTCCAG TGGAACCAGG
            181 TTTGCTGTGA ACTTTCAGAC TGGCTTCAGT GGAAATGACA TTGCCTTCCA CTTCAACCCT
            241 CGGTTTGAAG ATGGAGGGTA CGTGGTGTGC AACACGAGGC AGAACGGAAG CTGGGGGCCC
            301 GAGGAGAGGA AGACACACAT GCCTTTCCAG AAGGGGATGC CCTTTGACCT CTGCTTCCTG
            361 GTGCAGAGCT CAGATTTCAA GGTGATGGTG AACGGGATCC TCTTCGTGCA GTACTTCCAC
            421 CGCGTGCCCT TCCACCGTGT GGACACCATC TCCGTCAATG GCTCTGTGCA GCTATCCTAC
            481 ATCAGCTTCC AGCCTCCCGG CGTGTGGCCT GCCAACCCGG CTCCCATTAC CCAGACAGTC
            541 ATCCACACAG TGCAAAGCGC CCCTGGACAG ATGTTCTCTA CTCCCGCCAT CCCACCTATG
            601 ATGTACCCCC ACCCCGCCTA TCCGATGCCT TTCATCACCA CCATTCTGGG AGGGCTGTAC
            661 CCATCCAAGT CCATCCTCCT GTCAGGCACT GTCCTGCCCA GTGCTCAGAG GTTCCACATC
            721 AACCTGTGCT CTGGGAACCA CATCGCCTTC CACCTGAACC CCCGATTTGA TGAGAATGCT
            781 GTGGTCCGCA ACACCCAGAT CGACAACTCC TGGGGGTCTG AGGAGCGAAG TCTGCCCCGA
            841 AAAATGCCCT TCGTCCGTGG CCAGAGCTTC TCAGTGTGGA TCTTGTGTGA AGCTCACTGC
            901 CTCAAGGTGG CCGTGGATGG TCAGCACCTG TTTGAATACT ACCATCGCCT GAGGAACCTG
            961 CCCACCATCA ACAGACTGGA AAGTGGGGGG GCGACATCCA GCTGACCCAT GTGCAGACAT
           1021 AGCTCGAGCG GGCCGCATCG TGACTGACTG ACGATCTGCC TCCGCGCGTT TCGGTGATGA
           1081 CGGTGAAAAA CTCTGACACA TGCCAGCTCC CCGGAGACCG GATCACAGCC TTGATCCTGG
          1141 TTAAAGACGG GGA
          //
          Primer: pGEX-R
          Sequence file: RDB13756_A6A4b.seq check
          >04223_04223_A6A4_3_pGEX-R_F12_18.ab1
              1 TCATACGTAA CGCGCGAGGC AGATCGTCAG TCAGTCACGA TGCGGCCGCT CGAGCTATGT
             61 CTGCACATGG GTCAGCTGGA TGTCGCCCCC CACTTCCAGT CTGTTGATGG TGGGCAGGTT
            121 CCTCAGGCGA TGGTAGTATT CAAACAGGTG CTGACCATCC ACGGCCACCT TGAGGCAGTG
            181 AGCTTCACAC AAGATCCACA CTGAGAAGCT CTGGCCACGG ACGAAGGGCA TTTTTCGGGG
            241 CAGACTTCGC TCCTCAGACC CCCAGGAGTT GTCGATCTGG GTGTTGCGGA CCACAGCATT
            301 CTCATCAAAT CGGGGGTTCA GGTGGAAGGC GATGTGGTTC CCAGAGCACA GGTTGATGTG
            361 GAACCTCTGA GCACTGGGCA GGACAGTGCC TGACAGGAGG ATGGACTTGG ATGGGTACAG
            421 CCCTCCCAGA ATGGTGGTGA TGAAAGGCAT CGGATAGGCG GGGTGGGGGT ACATCATAGG
            481 TGGGATGGCG GGAGTAGAGA ACATCTGTCC AGGGGCGCTT TGCACTGTGT GGATGACTGT
            541 CTGGGTAATG GGAGCCGGGT TGGCAGGCCA CACGCCGGGA GGCTGGAAGC TGATGTAGGA
            601 TAGCTGCACA GAGCCATTGA CGGAGATGGT GTCCACACGG TGGAAGGGCA CGCGGTGGAA
            661 GTACTGCACG AAGAGGATCC CGTTCACCAT CACCTTGAAA TCTGAGCTCT GCACCAGGAA
            721 GCAGAGGTCA AAGGGCATCC CCTTCTGGAA AGGCATGTGT GTCTTCCTCT CCTCGGGCCC
            781 CCAGCTTCCG TTCTGCCTCG TGTTGCACAC CACGTACCCT CCATCTTCAA ACCGAGGGTT
            841 GAAGTGGAAA GGCAATGTCA TTTCCACTGA AGCCAGTCTG AAAGTTCACA GCAAACCTGG
            901 TTTCCACTGG AGCTGAGAAC GGTCCCATTG ACAGTGATCT GAAGTCCGTC CTGAGACCTC
            961 CTTTGAATAG TCCCAGAAAA GGGGACAGCT GGACTCAGTA GGGAGCCCTG GACTGCTGAG
           1021 CCATGGGAAT TCCTGGGGAA TCCACGCGAC AGATCGATTT TGGAGAATGT CGCCACACGA
          1081 ACGTTGCTGC AGCCTGCAAG GCCATGCTAA TACCTTGCTT GGAATTTCAG TTACCTTA
          //
          Primer: M13_-40
          Sequence file: RDB13756_A6A4c.seq check
          >04223_04223_A6A4_3_M13(-40)_H10_22.ab1
              1 TCTGAACGCG GGGAATCCGG TAATCATGGT TCATAGCTGT TTCCTGTGTG AAATTGTTAT
             61 CCGCTCACAA TTCCACACAA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
            121 CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
            181 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG
            241 TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT
            301 TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC
            361 GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT
            421 CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA
            481 TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT
            541 TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG
            601 CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG
            661 CCGAGACAGA ACTTAAATGG GCCCGCTAAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC
            721 CAAGATGCTC CACGCCCAGT CGCGTACCGT CTTCAATGGG AGAAAATAAT ACTGTTGATG
            781 GGTGTCTGGT CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCAAC
            841 AGCAATGGCA TCCTGGTCAT CCAGCGGAAT AGTTAATGAT CAACCCAACT GACGCGTTGC
            901 GCGAGAAGAT TGTGCACCCG CCGCTTTAAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG
            961 ACACCAACCA CGCTGGGCAC CCAGTTGATC GGGCGCGAGA ATTTAATCGC CGCGGACCAA
           1021 TTTTGCGAAC GGCGCGTGCA GGGCCAGAAC CTGGAAAGTG CCAACGCCAA TCAACCAACC
           1081 GAACTGTTTG ACCGACCAGT TGGTTGTGCC ACGCGGGTGG AAAAAGTAAA TCAGCTTCGG
          1141 CAATCGCCGA CTACCACTAT TTTCCGACGG GTATTATCGC CGAAAACACG GTTG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsushita, N., Requirement of divalent galactoside-binding activity of ecalectin/galectin-9 for eosinophil chemoattraction. J. Biol. Chem., 275, 8355-8360 (2000). PMID 10722666.
          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl