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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-G12CT-1b (#RDB08430)

Bacteria expression vector of human galectin-12 (C-terminal CRD with a part of the linker peptide, G12CT), GST fusion protein

Clone info. Bacterial expression clone of a C-terminal CRD of human galectin-12 with a part of linker peptide.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-12 cDNA
Depositor|Developer Nishi, Nozomu |
 
Sequence RDB08430z.seq

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08430 pGEX-G12CT-1b DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-G12CT-1b was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08430).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08430_A8Bsp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB08430_A8Bsa.seq check
>08430_08430_A8Bs_pGEX-F2_E01_05_ABI08.ab1
    1 AACATAAATG TTTTGTTTTA AAAAACGGTA TTGAAGCTAT CCCACAAATT GATAAGTACT
   61 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
  121 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CCCAGCTGGA CATCCTTTCC
  181 TGCTGATGAG CCCCAGGCTG GAGGTGCCCT GCTCACATGC TCTTCCCCAG GGTCTCTCGC
  241 CTGGGCAGGT CATCATAGTA CGGGGACTGG TCTTGCAAGA GCCGAAGCAT TTTACTGTGA
  301 GCCTGAGGGA CCAGGCTGCC CATGCTCCTG TGACACTCAG GGCCTCCTTC GCAGACAGAA
  361 CTCTGGCCTG GATCTCCCGC TGGGGGCAGA AGAAACTGAT CTCAGCCCCC TTCCTCTTTT
  421 ACCCCCAGAG ATTCTTTGAG GTGCTGCTCC TGTTCCAGGA GGGAGGGCTG AAGCTGGCGC
  481 TCAATGGGCA GGGGCTGGGG GCCACCAGCA TGAACCAGCA GGCCCTGGAG CAGCTGCGGG
  541 AGCTCCGGAT CAGTGGAAGT GTCCAGCTCT ACTGTGTCCA CTCCTGAGAA TTCCCGGGTC
  601 GACTCGAGCG GCCGCATCGT GACTGACTGA CGATCTGCCT CGCGCGTTTC GGTGATGACG
  661 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG
  721 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG
  781 CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATAAATT CTTGAAGACG AAAGGGCCTC
  841 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT
  901 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA
  961 AATATGTATC CGCTCATGAG ACATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGAA
 1021 GAGTATGAGT ATTCAACATT TCCGGTGTCG CCTTATTCCC TTTTTTTTGC GGCATTTTGG
 1081 CCTTCCTGTT TTTGCTCACC AGAAACGCTG GTGAAAGGTA AAAGATGCTG AGGATCAGTG
1141 GGGTGGCACC GAGCTGGGGT TTACAATTCG GAACTGGGAA TTCTCAAACA AGCCG
//
Primer: M13_-40
Sequence file: RDB08430_A2Cmb.seq check
>08430_08430_A2Cm_M13_-40_A02_02_ABI24.ab1
    1 TAGGCAAGGG AATCCGTATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
   61 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
  121 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
  181 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
  241 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC
  301 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC
  361 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC
  421 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC
  481 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT
  541 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG
  601 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC
  661 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC
  721 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAAAT ACTGTTGATG GGTGTCTGGT
  781 CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA GCAATGGCAT
  841 CCTGGTCATC CAGCGGATAG TTAAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG
  901 TGCACCGCCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT
  961 GGCACCCAGT TTGATCGGGC GCCGAGATTT AAATCGCCGG CGACAATTTG CGACGGGCGG
 1021 CGGTGCAGGG GCCAGAACTG GAGGTGGGCA CGCCCAATCA CCAACGACTG TTGCCCCGCC
1081 AGTTGATGTT GCCACCCGGG GTTGGGATAG GTAATTCCAG CTCTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]