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pGEX-G12CT-1b

Bacteria expression vector of human galectin-12 (C-terminal CRD with a part of the linker peptide, G12CT), GST fusion protein

Catalog number RDB08430
Resource name pGEX-G12CT-1b
Clone info. Bacterial expression clone of a C-terminal CRD of human galectin-12 with a part of linker peptide.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-12 cDNA
Depositor|Developer Nishi, Nozomu |
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External Database
Human galectin-12

          Reference sequence
            

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
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          RDB08430 pGEX-G12CT-1b DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08430z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08430_A8Bsp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB08430_A8Bsa.seq check
          >08430_08430_A8Bs_pGEX-F2_E01_05_ABI08.ab1
              1 AACATAAATG TTTTGTTTTA AAAAACGGTA TTGAAGCTAT CCCACAAATT GATAAGTACT
             61 TGAAATCCAG CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
            121 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CCCAGCTGGA CATCCTTTCC
            181 TGCTGATGAG CCCCAGGCTG GAGGTGCCCT GCTCACATGC TCTTCCCCAG GGTCTCTCGC
            241 CTGGGCAGGT CATCATAGTA CGGGGACTGG TCTTGCAAGA GCCGAAGCAT TTTACTGTGA
            301 GCCTGAGGGA CCAGGCTGCC CATGCTCCTG TGACACTCAG GGCCTCCTTC GCAGACAGAA
            361 CTCTGGCCTG GATCTCCCGC TGGGGGCAGA AGAAACTGAT CTCAGCCCCC TTCCTCTTTT
            421 ACCCCCAGAG ATTCTTTGAG GTGCTGCTCC TGTTCCAGGA GGGAGGGCTG AAGCTGGCGC
            481 TCAATGGGCA GGGGCTGGGG GCCACCAGCA TGAACCAGCA GGCCCTGGAG CAGCTGCGGG
            541 AGCTCCGGAT CAGTGGAAGT GTCCAGCTCT ACTGTGTCCA CTCCTGAGAA TTCCCGGGTC
            601 GACTCGAGCG GCCGCATCGT GACTGACTGA CGATCTGCCT CGCGCGTTTC GGTGATGACG
            661 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG
            721 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG
            781 CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATAAATT CTTGAAGACG AAAGGGCCTC
            841 GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT
            901 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA
            961 AATATGTATC CGCTCATGAG ACATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGAA
           1021 GAGTATGAGT ATTCAACATT TCCGGTGTCG CCTTATTCCC TTTTTTTTGC GGCATTTTGG
           1081 CCTTCCTGTT TTTGCTCACC AGAAACGCTG GTGAAAGGTA AAAGATGCTG AGGATCAGTG
          1141 GGGTGGCACC GAGCTGGGGT TTACAATTCG GAACTGGGAA TTCTCAAACA AGCCG
          //
          Primer: M13_-40
          Sequence file: RDB08430_A2Cmb.seq check
          >08430_08430_A2Cm_M13_-40_A02_02_ABI24.ab1
              1 TAGGCAAGGG AATCCGTATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
             61 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
            121 GTGAGCTAAC TTACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
            181 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
            241 CGCCAGGGTG GTTTTTCTTT TCACCAGTGA GACGGGCAAC AGCTGATTGC CCTTCACCGC
            301 CTGGCCCTGA GAGAGTTGCA GCAAGCGGTC CACGCTGGTT TGCCCCAGCA GGCGAAAATC
            361 CTGTTTGATG GTGGTTAACG GCGGGATATA ACATGAGCTG TCTTCGGTAT CGTCGTATCC
            421 CACTACCGAG ATATCCGCAC CAACGCGCAG CCCGGACTCG GTAATGGCGC GCATTGCGCC
            481 CAGCGCCATC TGATCGTTGG CAACCAGCAT CGCAGTGGGA ACGATGCCCT CATTCAGCAT
            541 TTGCATGGTT TGTTGAAAAC CGGACATGGC ACTCCAGTCG CCTTCCCGTT CCGCTATCGG
            601 CTGAATTTGA TTGCGAGTGA GATATTTATG CCAGCCAGCC AGACGCAGAC GCGCCGAGAC
            661 AGAACTTAAT GGGCCCGCTA ACAGCGCGAT TTGCTGGTGA CCCAATGCGA CCAGATGCTC
            721 CACGCCCAGT CGCGTACCGT CTTCATGGGA GAAAATAAAT ACTGTTGATG GGTGTCTGGT
            781 CAGAGACATC AAGAAATAAC GCCGGAACAT TAGTGCAGGC AGCTTCCACA GCAATGGCAT
            841 CCTGGTCATC CAGCGGATAG TTAAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG
            901 TGCACCGCCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT
            961 GGCACCCAGT TTGATCGGGC GCCGAGATTT AAATCGCCGG CGACAATTTG CGACGGGCGG
           1021 CGGTGCAGGG GCCAGAACTG GAGGTGGGCA CGCCCAATCA CCAACGACTG TTGCCCCGCC
          1081 AGTTGATGTT GCCACCCGGG GTTGGGATAG GTAATTCCAG CTCTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl