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pET-CSG1-1a

Bacteria expression vector of human galectin-1 in which all Cys residues are substituted with Ser residues (G1C3,17,43,61,89,131S: CSG1), tag-free protein

Catalog number RDB08449
Resource name pET-CSG1-1a
Clone info. Bacterial expression clone of human galectin-1 with C3,17,43,61,89,131S mutations.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pET-11a (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Human galectin-1 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank
External Database
Human galectin-1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
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          RDB08449 pET-CSG1-1a Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence RDB08449z.seq

          Original reference

          original Nishi, N., Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1. Glycobiology 18: 1065-1073 (2008). PMID 18796645.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Nishi, N., Functional and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1. Glycobiology 18: 1065-1073 (2008). PMID 18796645.
          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.05.17

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