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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

mIGEH

Mouse cDNA for IgE H chain.

Catalog number RDB15983
Resource name mIGEH
Alternative name Mouse IgE H chain
Clone info. Mouse cDNA for IgE H chain in pGEM-T Easy vector, cDNA is inserted in a reverse orientation in relation to the T7 promoter of the plasmid. Please note that this clone contains no signal peptide sequence.
Vector backbone pGEM-T Easy (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse IgE H chain cDNA
Reference of insert sequence LC387253 (DDBJ accession number)
Depositor|Developer Nishi, Nozomu |
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mouse IgE H chain
            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017)。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

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            RDB15983 mIGEH DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The mIGEH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15983).

            Reference section:

            Nakakita, S., Itoh, A., Nakakita, Y., Nonaka, Y., Ogawa, T., Nakamura, T., Nishi, N., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15983_A8Fip1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ColE1ori_F ()
            Region: ori,insert5'
            Sequence file: RDB15983_A8Fic.seq check
            >D05595A1-1_A8Fi_ColE1ori_F_C01_07_ABI24.ab1
                1 GGTAGGTAGA CGGATCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
               61 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
              121 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT
              181 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA
              241 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG
              301 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC
              361 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
              421 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC
              481 CATGATTACG CCAAGCTATT TAGGTGACAC TATAGAATAC TCAAGCTATG CATCCAACGC
              541 GTTGGGAGCT CTCCCATATG GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTGAGG
              601 TCCAGCTGCA GCAGTCTGGG GCTGAGCTGG TGAGGCCTGG GTCCTCAGTG AAGATGTCCT
              661 GCAAGAGTTC TGGATATACA TTCACAAGCT ACGGTATAAA CTGGGTGAAG CAGAGGCCTG
              721 GACAGGGCCT GGAATGGATT GGATATATTT ATATTGGATA TGGTTATATT GAGTATAATG
              781 AGAAGTTCAA GGGCAAGGCC ACACTGACTT CAGACACATC CTCCAGGACA GCCTACATGC
              841 AACTCAGCAG CCTGACATCT GAGGACTCTG CAATCTATTT TCTGTGCAAG ATGGGGCTTA
              901 ATCTTTGCTA TGGACTACTG GGGGTCAAGG AACCTCAGGT CACCGTCTCC TCAGCCTCTA
              961 TCAGGAACCC CTCAGCTCTA CCCCCTTGAG CCCTGTAAAG GCACTGCTTC CATGACCCTG
             1021 GGCTGCCTGG TAAAGGACTA CTTTCCCTGA TCCTTGTGAA CTGTTGACCC TGTTATTCAG
            1081 AACTTCCCTG AACATGAAGC AC
            //
            Primer: M13_-40 ()
            Region: insert3'
            Sequence file: RDB15983_A8Fid.seq check
            >D05595A1-1_A8Fi_M13_-40_B01_02_ABI08.ab1
                1 AGGCAAGTTA ATTGTAATAC GACTCACTAT AGGGCGAATT GGGCCCGACG TCGCATGCTC
               61 CCGGCCGCCA TGGCGGCCGC GGGAATTCGA TTCTAGGAGG GACGGAGGGA GGTGTTACCA
              121 AGGCTCGTGG ATATTGTTTT CTCCAGTTTC CTGGGTTTCT GAAGTGCCTC ATGGATCACT
              181 TGGCAGGTGA ACTGTTTTCT CTGTGTCCAG AGTGTCTTGG CGACCTCTAG GCGACTGAAG
              241 ATGAAGAAGC CTTGATTGGA GCCATTGGAT TTCAGGGGTG TTGTGGTACT GTGATGGCTG
              301 TTTGAGATCA GTTTGCCATC CTCCAGCCAC TGCACAGAGA TATCCTCAGG GAAGAAGTTC
              361 TGGATCAAGC AGGTGAGTGT GCGTTTGTCC TCGCTCTCCT CCTCTGGTGG TGGGAACACA
              421 TATACCTCGG GGGCTGAGCG CTGGCCTGGG GTCTTGGTGA TGGAACGCAC AATGGGCTTG
              481 GGAAAATCAG GGTGGTCCAC TATGCACTGA TAGCCGTAGC CTTCAATCCA GTCCTTGGCA
              541 ACTACAGGCA GGATGGAGGT GATACTGGTT GTGGCGTTAT GGTGGTGCTT AGTGTACCAC
              601 TGGGATGCTG AGACTGGAGT CTTCTTCTCT TGGTTCCACG TCACATTGAC ATTCTTCTCG
              661 CTTTCCAGGT CCACCACCAG ACAGGTAAGC TTGGGAGCAC CGTTTTGATA CAGGTCCAGG
              721 GGGCTGGGTG GGATCAGGTA GGTAATCACA CCCCGTGGCT CATGATCTGG GCATCTCCGA
              781 GTGTGGGCCA AATAGTCTAC GCCTTGGGAG GTGACCTTGC AGGTGAAGGT GCTTTCAGAC
              841 ATCCATTGCT GCTCAGTGAT GTTGAGTTTA CTGCAGGTAG AGGCTAGTTT GCCTTCCTCC
              901 TTGATTAGAA CAGTTTGTGC AAGTGTATCA GTTATCTCCC GATCGTCCAT TAGCCAGCTG
              961 ACAGAGACAT CATTTAGGAT GTGGCCATAA ATGAAGCAGT ACAGCTGGAT GGTGGAGTGG
             1021 AAAGCATTGG GGGTCGCAGG ATGAATGGAG TAGCTCTAGT GGGCTCAGTG ATGTTGACAG
             1081 GTCGAACCTA GATAGTCTAC TTTCGTTGGA TGATGGAGAT GTGTCACGTT GCATGTGAAG
            1141 TCTGGCGACT GCCAGCTGAT CAACTGGGTA CG
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205. [link to RRC of NBRP]

            2023.08.17

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