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mIGEH

Mouse cDNA for IgE H chain.

Catalog number RDB15983
Resource name mIGEH
Alternative name Mouse IgE H chain
Clone info. Mouse cDNA for IgE H chain in pGEM-T Easy vector, cDNA is inserted in a reverse orientation in relation to the T7 promoter of the plasmid. Please note that this clone contains no signal peptide sequence.
Vector backbone pGEM-T Easy (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse IgE H chain cDNA
Reference of nucleotide sequence LC387253 (DDBJ accession number)
Depositor Nishi, Nozomu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).
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RDB15983 mIGEH DNA solution

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References and tips

Electronic file

Original reference

original Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15983_A8Fip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: ori,insert5' | Primer: ColE1ori_F | Sequence: RDB15983_A8Fic.seq
>D05595A1-1_A8Fi_ColE1ori_F_C01_07_ABI24.ab1
    1 GGTAGGTAGA CGGATCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
   61 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC
  121 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT
  181 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA
  241 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG
  301 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC
  361 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG
  421 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC
  481 CATGATTACG CCAAGCTATT TAGGTGACAC TATAGAATAC TCAAGCTATG CATCCAACGC
  541 GTTGGGAGCT CTCCCATATG GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTGAGG
  601 TCCAGCTGCA GCAGTCTGGG GCTGAGCTGG TGAGGCCTGG GTCCTCAGTG AAGATGTCCT
  661 GCAAGAGTTC TGGATATACA TTCACAAGCT ACGGTATAAA CTGGGTGAAG CAGAGGCCTG
  721 GACAGGGCCT GGAATGGATT GGATATATTT ATATTGGATA TGGTTATATT GAGTATAATG
  781 AGAAGTTCAA GGGCAAGGCC ACACTGACTT CAGACACATC CTCCAGGACA GCCTACATGC
  841 AACTCAGCAG CCTGACATCT GAGGACTCTG CAATCTATTT TCTGTGCAAG ATGGGGCTTA
  901 ATCTTTGCTA TGGACTACTG GGGGTCAAGG AACCTCAGGT CACCGTCTCC TCAGCCTCTA
  961 TCAGGAACCC CTCAGCTCTA CCCCCTTGAG CCCTGTAAAG GCACTGCTTC CATGACCCTG
 1021 GGCTGCCTGG TAAAGGACTA CTTTCCCTGA TCCTTGTGAA CTGTTGACCC TGTTATTCAG
1081 AACTTCCCTG AACATGAAGC AC
//
Region: insert3' | Primer: M13_-40 | Sequence: RDB15983_A8Fid.seq
>D05595A1-1_A8Fi_M13_-40_B01_02_ABI08.ab1
    1 AGGCAAGTTA ATTGTAATAC GACTCACTAT AGGGCGAATT GGGCCCGACG TCGCATGCTC
   61 CCGGCCGCCA TGGCGGCCGC GGGAATTCGA TTCTAGGAGG GACGGAGGGA GGTGTTACCA
  121 AGGCTCGTGG ATATTGTTTT CTCCAGTTTC CTGGGTTTCT GAAGTGCCTC ATGGATCACT
  181 TGGCAGGTGA ACTGTTTTCT CTGTGTCCAG AGTGTCTTGG CGACCTCTAG GCGACTGAAG
  241 ATGAAGAAGC CTTGATTGGA GCCATTGGAT TTCAGGGGTG TTGTGGTACT GTGATGGCTG
  301 TTTGAGATCA GTTTGCCATC CTCCAGCCAC TGCACAGAGA TATCCTCAGG GAAGAAGTTC
  361 TGGATCAAGC AGGTGAGTGT GCGTTTGTCC TCGCTCTCCT CCTCTGGTGG TGGGAACACA
  421 TATACCTCGG GGGCTGAGCG CTGGCCTGGG GTCTTGGTGA TGGAACGCAC AATGGGCTTG
  481 GGAAAATCAG GGTGGTCCAC TATGCACTGA TAGCCGTAGC CTTCAATCCA GTCCTTGGCA
  541 ACTACAGGCA GGATGGAGGT GATACTGGTT GTGGCGTTAT GGTGGTGCTT AGTGTACCAC
  601 TGGGATGCTG AGACTGGAGT CTTCTTCTCT TGGTTCCACG TCACATTGAC ATTCTTCTCG
  661 CTTTCCAGGT CCACCACCAG ACAGGTAAGC TTGGGAGCAC CGTTTTGATA CAGGTCCAGG
  721 GGGCTGGGTG GGATCAGGTA GGTAATCACA CCCCGTGGCT CATGATCTGG GCATCTCCGA
  781 GTGTGGGCCA AATAGTCTAC GCCTTGGGAG GTGACCTTGC AGGTGAAGGT GCTTTCAGAC
  841 ATCCATTGCT GCTCAGTGAT GTTGAGTTTA CTGCAGGTAG AGGCTAGTTT GCCTTCCTCC
  901 TTGATTAGAA CAGTTTGTGC AAGTGTATCA GTTATCTCCC GATCGTCCAT TAGCCAGCTG
  961 ACAGAGACAT CATTTAGGAT GTGGCCATAA ATGAAGCAGT ACAGCTGGAT GGTGGAGTGG
 1021 AAAGCATTGG GGGTCGCAGG ATGAATGGAG TAGCTCTAGT GGGCTCAGTG ATGTTGACAG
 1081 GTCGAACCTA GATAGTCTAC TTTCGTTGGA TGATGGAGAT GTGTCACGTT GCATGTGAAG
1141 TCTGGCGACT GCCAGCTGAT CAACTGGGTA CG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205.

2019.09.06

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