Please review the QC test results indicated by icon below as well as clone information before placing your order.

mIGEH (#RDB15983)

Mouse cDNA for IgE H chain.

Alternative name	Mouse IgE H chain
Clone info.	Mouse cDNA for IgE H chain in pGEM-T Easy vector, cDNA is inserted in a reverse orientation in relation to the T7 promoter of the plasmid. Please note that this clone contains no signal peptide sequence.
Vector backbone	pGEM-T Easy (plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	mouse IgE H chain cDNA
Reference of insert sequence	LC387253 (DDBJ accession number)
Depositor|Developer	Nishi, Nozomu |

 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (J. Biol. Chem. 291(2): 968-979, 2016; Biosci. Biotechnol. Biochem. 81(11): 2098-2104, 2017).

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15983	mIGEH	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

---

How to cite this biological resource

Materials & Methods section:

The mIGEH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15983).

Reference section:

Nakakita, S., Itoh, A., Nakakita, Y., Nonaka, Y., Ogawa, T., Nakamura, T., Nishi, N., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

---

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15983_A8Fip1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ColE1ori_F () Region: ori,insert5' Sequence file: RDB15983_A8Fic.seq
>D05595A1-1_A8Fi_ColE1ori_F_C01_07_ABI24.ab1     1 GGTAGGTAGA CGGATCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG    61 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC   121 TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT   181 ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA   241 GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG   301 ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC   361 GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG   421 GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC   481 CATGATTACG CCAAGCTATT TAGGTGACAC TATAGAATAC TCAAGCTATG CATCCAACGC   541 GTTGGGAGCT CTCCCATATG GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTGAGG   601 TCCAGCTGCA GCAGTCTGGG GCTGAGCTGG TGAGGCCTGG GTCCTCAGTG AAGATGTCCT   661 GCAAGAGTTC TGGATATACA TTCACAAGCT ACGGTATAAA CTGGGTGAAG CAGAGGCCTG   721 GACAGGGCCT GGAATGGATT GGATATATTT ATATTGGATA TGGTTATATT GAGTATAATG   781 AGAAGTTCAA GGGCAAGGCC ACACTGACTT CAGACACATC CTCCAGGACA GCCTACATGC   841 AACTCAGCAG CCTGACATCT GAGGACTCTG CAATCTATTT TCTGTGCAAG ATGGGGCTTA   901 ATCTTTGCTA TGGACTACTG GGGGTCAAGG AACCTCAGGT CACCGTCTCC TCAGCCTCTA   961 TCAGGAACCC CTCAGCTCTA CCCCCTTGAG CCCTGTAAAG GCACTGCTTC CATGACCCTG  1021 GGCTGCCTGG TAAAGGACTA CTTTCCCTGA TCCTTGTGAA CTGTTGACCC TGTTATTCAG 1081 AACTTCCCTG AACATGAAGC AC //
Primer: M13_-40 () Region: insert3' Sequence file: RDB15983_A8Fid.seq
>D05595A1-1_A8Fi_M13_-40_B01_02_ABI08.ab1     1 AGGCAAGTTA ATTGTAATAC GACTCACTAT AGGGCGAATT GGGCCCGACG TCGCATGCTC    61 CCGGCCGCCA TGGCGGCCGC GGGAATTCGA TTCTAGGAGG GACGGAGGGA GGTGTTACCA   121 AGGCTCGTGG ATATTGTTTT CTCCAGTTTC CTGGGTTTCT GAAGTGCCTC ATGGATCACT   181 TGGCAGGTGA ACTGTTTTCT CTGTGTCCAG AGTGTCTTGG CGACCTCTAG GCGACTGAAG   241 ATGAAGAAGC CTTGATTGGA GCCATTGGAT TTCAGGGGTG TTGTGGTACT GTGATGGCTG   301 TTTGAGATCA GTTTGCCATC CTCCAGCCAC TGCACAGAGA TATCCTCAGG GAAGAAGTTC   361 TGGATCAAGC AGGTGAGTGT GCGTTTGTCC TCGCTCTCCT CCTCTGGTGG TGGGAACACA   421 TATACCTCGG GGGCTGAGCG CTGGCCTGGG GTCTTGGTGA TGGAACGCAC AATGGGCTTG   481 GGAAAATCAG GGTGGTCCAC TATGCACTGA TAGCCGTAGC CTTCAATCCA GTCCTTGGCA   541 ACTACAGGCA GGATGGAGGT GATACTGGTT GTGGCGTTAT GGTGGTGCTT AGTGTACCAC   601 TGGGATGCTG AGACTGGAGT CTTCTTCTCT TGGTTCCACG TCACATTGAC ATTCTTCTCG   661 CTTTCCAGGT CCACCACCAG ACAGGTAAGC TTGGGAGCAC CGTTTTGATA CAGGTCCAGG   721 GGGCTGGGTG GGATCAGGTA GGTAATCACA CCCCGTGGCT CATGATCTGG GCATCTCCGA   781 GTGTGGGCCA AATAGTCTAC GCCTTGGGAG GTGACCTTGC AGGTGAAGGT GCTTTCAGAC   841 ATCCATTGCT GCTCAGTGAT GTTGAGTTTA CTGCAGGTAG AGGCTAGTTT GCCTTCCTCC   901 TTGATTAGAA CAGTTTGTGC AAGTGTATCA GTTATCTCCC GATCGTCCAT TAGCCAGCTG   961 ACAGAGACAT CATTTAGGAT GTGGCCATAA ATGAAGCAGT ACAGCTGGAT GGTGGAGTGG  1021 AAAGCATTGG GGGTCGCAGG ATGAATGGAG TAGCTCTAGT GGGCTCAGTG ATGTTGACAG  1081 GTCGAACCTA GATAGTCTAC TTTCGTTGGA TGATGGAGAT GTGTCACGTT GCATGTGAAG 1141 TCTGGCGACT GCCAGCTGAT CAACTGGGTA CG //

Please visit Sequencing and PCR primers for primer information.

---

References

Original, user report and related articles

original	Nakakita, S., Cooperative Interactions of Oligosaccharide and Peptide Moieties of a Glycopeptide Derived from IgE with Galectin-9. J. Biol. Chem. \291 (2): 968-979 (2016) PMID 26582205. [link to RRC of NBRP]

Resource search

Go!

Link

Other resources in our bank

---

External Database
mouse IgE H chain

---

Reference sequence
  

Tips

Featured content

---