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pGEX-G12NT-1b

Bacteria expression vector of human galectin-12 (N-terminal CRD with a part of the linker peptide, G12NT), GST fusion protein

Catalog number RDB08429
Resource name pGEX-G12NT-1b
Clone info. Bacterial expression clone of an N-terminal CRD of human galectin-12 (with a shorter N-terminal sequence) with a part of linker peptide.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-12 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank
External Database
Human galectin-12
          Reference sequence
            
           
          Sequence RDB08429z.seq

          Distribution information

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          RDB08429 pGEX-G12NT-1b DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGEX-G12NT-1b was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08429).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08429_A8Bsp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB08429_A8Bsa.seq check
          >08429_08429_A8Bs_pGEX-F2_C01_03_ABI08.ab1
              1 GATAAGGTTT TGGTTTTTAA AAAACGTTAT TGAAGCTATC CCACAAATTG ATAAGTACTT
             61 GAAATCCAGC AAGTATATAG CATGGCCTTT GCAGGGCTGG CAAGCCACGT TTGGTGGTGG
            121 CGACCATCCT CCAAAATCGG ATCTGGTTCC GCGTGGATCC ATGTCACCTG GAGAAAAACT
            181 GGACCCAATT CCTGACAGCT TCATTCTGCA ACCACCAGTC TTCCACCCGG TGGTTCCTTA
            241 TGTCACGACG ATTTTTGGAG GCCTGCATGC AGGCAAGATG GTCATGCTGC AAGGAGTGGT
            301 CCCTCTAGAT GCACACAGGT TTCAGGTGGA CTTCCAGTGT GGCTGCAGCC TGTGTCCCCG
            361 GCCAGATATC GCCTTCCACT TCAACCCTCG CTTCCATACC ACCAAGCCCC ATGTCATCTG
            421 CAACACCCTG CATGGTGGAC GCTGGCAAAG GGAGGCCCGG TGGCCCCACC TGGCCCTGCG
            481 AAGAGGCTCC AGCTTCCTCA TCCTCTTTCT CTTCGGGAAT GAGGAAGTGA AGGTGAGTGT
            541 GAATGGACAG CACTTTCTCC ACTTCCGCTA CCGGCTCCCA CTGTCTCATG TGGACACGCT
            601 GGGTATATTT GGTGACATCC TGGTAGAGGC TGTTGGATTC CTGAACATCA ATCCATTTGT
            661 GGAGGGCAGC TGAGAATTCC CGGGTCGACT CGAGCGGCCG CATCGTGACT GACTGACGAT
            721 CTGCTCGCGC GTTTCGGTGA TGACGGTGAA AACTCTGACC ATGCAGCTCC CGAGAACGTT
          781 CCACAGCCTT GTTCTGTAAG CCGGAATGGC CG
          //
          Primer: M13_-40
          Sequence file: RDB08429_A2Cmb.seq check
          >08429_08429_A2Cm_M13_-40_F01_16_ABI24.ab1
              1 AGGGCCAATC CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
             61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
            121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
            181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
            241 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
            301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
            361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
            421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
            481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
            541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
            601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
            661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
            721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
            781 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
            841 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC
            901 CGCCGCTTTA CAGGCTTCGA CGCGCTTCGT TCTACCATCG ACACCACACG CTGGCACCCA
            961 GTTGATCGGC GGCGGAAGAT TAATCGCCGC GACATTTGCG ACGCGGCGGT GCAGGGCAGA
           1021 CTGCAGGTGG CAACGCATCA GCACCGACTG TTTGCCGCAG TGTGTGCCAA GCGATGGATG
          1081 TATCAGCTCC GCATGCCGAT CACTTTTCGC GATTCGCAGA TCGTGCCTGA CCT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl