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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-G4NT

Bacteria expression vector of human galectin-4 (N-terminal CRD with a part of the linker peptide, G4NT), GST fusion protein

Catalog number RDB18237
Resource name pGEX-G4NT
Clone info. Bacterial expression clone of an N-terminal CRD of human galectin-4 with a part of linker peptide.
Comment Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone pGEX-4T-2 (Plasmid)
Gene/insert name Human galectin-4 cDNA
Depositor|Developer Nishi, Nozomu |
Other clones in our bank

External Database
Human galectin-4

          Reference sequence
            
           
          Sequence (partial) RDB18237pka01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.
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          RDB18237 pGEX-G4NT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18237_B0Gfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Region: GST, thrombin site, Insert 5'
          Sequence file: RDB18237_B0Gfa.seq check
          >D07204A1_B0Gf_1_pGEX-F2_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNANGTAT TGNNGCTATC CCNCAAATTG ATAAGTACTT
             61 GAAATCCAGN CAAGTATATA GCATGGCCTT TGCAGGGCTG GCAAGCCACG TTTGGTGGTG
            121 GCGACCATCC TCCAAAATCG GATCTGGTTC CGCGTGGATC CCCAGGAATT CCCATGGCCT
            181 ATGTCCCCGC ACCGGGCTAC CAGCCCACCT ACAACCCGAC GCTGCCTTAC TACCAGCCCA
            241 TCCCGGGCGG GCTCAACGTG GGAATGTCTG TTTACATCCA AGGAGTGGCC AGCGAGCACA
            301 TGAAGCGGTT CTTCGTGAAC TTTGTGGTTG GGCAGGATCC GGGCTCAGAC GTCGCCTTCC
            361 ACTTCAATCC GCGGTTTGAC GGCTGGGACA AGGTGGTCTT CAACACGTTG CAGGGCGGGA
            421 AGTGGGGCAG CGAGGAGAGG AAGAGGAGCA TGCCCTTCAA AAAGGGTGCC GCCTTTGAGC
            481 TGGTCTTCAT AGTCCTGGCT GAGCACTACA AGGTGGTGGT AAATGGAAAT CCCTTCTATG
            541 AGTACGGGCA CCGGCTTCCC CTACAGATGG TCACCCACCT GCAAGTGGAT GGGGATCTGC
            601 AACTTCAATC AATCAACTTC ATCGGAGGCC AGCCCCTCCG GCCCCAGGGA CCCCCGATGA
            661 TGCCACCTTA CTAACTCGAG CGGCCGCATC GTGACTGACT GACGATCTGC CTCGCGCGTT
            721 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC
            781 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCANCGGGN GTTGGNGGGT
            841 GTCGGGGCGC AGCCATGACC CANNCACGTA GCGATAGCGG ANTGTATAAT TCTTGAAGAN
            901 NAAAGGGCCT CGNGATACGC CTANTTTTAT ANGTTAATGN CNTGANNATA ATGGTTTCNT
            961 ANANGNNNNN TGGNNCTTTT TNNGGGAAAT GTGCGCNNNN CCCCTATTNN NTTATTTTTN
          1021 NNAANNNNNN NNNN
          //
          Primer: pJB8-Forward
          Region: Insert 3'
          Sequence file: RDB18237_B0Gfb.seq check
          >D07204A1_B0Gf_1_pJB8-Forward_H01_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNNGNNNNN GGCTGNGCCC CGACNCCCGC NNACNCCCGC
             61 TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT
            121 CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGAT
            181 CGTCAGTCAG TCACGATGCG GCCGCTCGAG TTAGTAAGGT GGCATCATCG GGGGTCCCTG
            241 GGGCCGGAGG GGCTGGCCTC CGATGAAGTT GATTGATTGA AGTTGCAGAT CCCCATCCNC
            301 TTGCNGGTGG GTGANCATCN NNNNNGGAAG CCGGNGCCCG TACTCNTANA AGGGATTTCC
          361 ATTTACCNCC NCCNNGTANN GCTCNNNNNG NACTATGANN ANNANNNNNN NNNN
          //
          Primer: M13_-40
          Region: lacI
          Sequence file: RDB18237_B0Gfc.seq check
          >D07204A1_B0Gf_1_M13_-40_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTGGTNNTA GCTGTTTCCT GTGTGAAATT GTTATCCGCT
             61 CNCAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG
            121 AGTGAGCTAA CTTACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT
            181 GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG
            241 GCGCCAGGGT GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG CCCTTCACCG
            301 CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC AGGCGAAAAT
            361 CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT GTCTTCGGTA TCGTCGTATC
            421 CCACTACCGA GATATCCGCA CCAACGCGCA GCCCGGACTC GGTAATGGCG CGCATTGCGC
            481 CCAGCGCCAT CTGATCGTTG GCAACCAGCA TCGCAGTGGG AACGATGCCC TCATTCAGCA
            541 TTTGCATGGT TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT TCCGCTATCG
            601 GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA CGCGCCGAGA
            661 CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG ACCCAATGCG ACCAGATGCT
            721 CCACGCCCAG TCGCGTACCG TCTTCATGGG AGAAAATAAT ACTGTTGATG GGTGTCTGGT
            781 CAGAGACATC AAGAAATAAC GCCGGAANAT TAGTGCANGC AGCTTCCACA GCAATGNCAT
            841 NCTGGNCATC CAGCGGANAG TTAANGATCA GCCCACNGAN GCGTTGCNCG ANAANANTGT
            901 GCNCCGNCNC TTTNCNGGNT TCNANGCNNC TNCNNNNTNN NNTCGACANN NNNNNNCTNG
          961 NNNNNNNNNG NNNNNNNNNA NANTNNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.07.27

          GNP_filter3_RDBDEP_html_220727.pl