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pGEX-G12-1a

Bacteria expression vector of human galectin-12, GST fusion protein

Catalog number RDB08428
Resource name pGEX-G12-1a
Clone info. Bacterial expression clone of human galectin-12 (with a shorter N-terminal sequence).
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-12 cDNA
Depositor|Developer Nishi, Nozomu |
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External Database
Human galectin-12

          Reference sequence
            

          Distribution information

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          RDB08428 pGEX-G12-1a DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08428z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08428_A8Bsp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB08428_A8Bsa.seq check
          >08428_08428_A8Bs_pGEX-F2_A01_01_ABI08.ab1
              1 CTTTTTAGTT TTGGTTTTAA AAACGTATTG AAGCTATCCC ACAAAATTGA TAAGTACTTG
             61 AAATCCAGCA AGTATATAGC ATGGCCTTTG CAGGGCTGGC AAGCCACGTT TGGTGGTGGC
            121 GACCATCCTC CAAAATCGGA TCTGGTTCCG CGTGGATCCA TGTCACCTGG AGAAAAACTG
            181 GACCCAATTC CTGACAGCTT CATTCTGCAA CCACCAGTCT TCCACCCGGT GGTTCCTTAT
            241 GTCACGACGA TTTTTGGAGG CCTGCATGCA GGCAAGATGG TCATGCTGCA AGGAGTGGTC
            301 CCTCTAGATG CACACAGGTT TCAGGTGGAC TTCCAGTGTG GCTGCAGCCT GTGTCCCCGG
            361 CCAGATATCG CCTTCCACTT CAACCCTCGC TTCCATACCA CCAAGCCCCA TGTCATCTGC
            421 AACACCCTGC ATGGTGGACG CTGGCAAAGG GAGGCCCGGT GGCCCCACCT GGCCCTGCGA
            481 AGAGGCTCCA GCTTCCTCAT CCTCTTTCTC TTCGGGAATG AGGAAGTGAA GGTGAGTGTG
            541 AATGGACAGC ACTTTCTCCA CTTCCGCTAC CGGCTCCCAC TGTCTCATGT GGACACGCTG
            601 GGTATATTTG GTGACATCCT GGTAGAGGCT GTTGGATTCC TGAACATCAA TCCATTTGTG
            661 GAGGGCAGCA GAGAGTACCC AGCTGGACAT CCTTTCCTGC TGATGAGCCC CAGGCTGGAG
            721 GTGCCCTGCT CACATGCTCT TCCCCAGGGT CTCTCGCCTG GGCAGGTCAT CATAGTACGG
            781 GGACTGGTCT TGCAAGAGCC GAAGCATTTT ACTGTGAGCC TGAGGGACCA GGCTGCCCAT
            841 GCTCCTGTGA CACTCAGGGC CTCCTTCGCA GACAGAACTC TGGCCTGGAT CTCCCGCTGG
            901 GGGCAGAAGA AACTGATCTC AGCCCCCTTC CTCTTTTACC CCCAGAGATT CTTTGAGGTG
            961 CTGCTCCTGT TCCAGGAGGG AGGGCTGAAG CTGGCGCTCA ATGGGCAGGG GCTGGGGGGC
           1021 CACCAGCATG GAACCAGCAG GCCCTGCAGC AGCTGCGGGG AGCTCGGGAT CAGTGGAAGT
           1081 GTCCAGCCTC TACTGTGTCC ACCTCCTGAG ATTTCCCGGG ATCGAACTCG AGCGGCCGCA
          1141 TCCGTGAACT GACTGAACGA TCCTGGCCTT CGGCCGCGCG GTTTTCGCGA TA
          //
          Primer: pGEX-RSEQ
          Sequence file: RDB08428_A8Bsb.seq check
          >08428_08428_A8Bs_pGEX-RSEQ_B01_02_ABI08.ab1
              1 GTTTATTCCG GAAAAAGCGC CGAAGGCAAG ATCGTCAGTC AGTCACGATG CGGCCGCTCG
             61 AGTCGACCCG GGAATTCTCA GGAGTGGACA CAGTAGAGCT GGACACTTCC ACTGATCCGG
            121 AGCTCCCGCA GCTGCTCCAG GGCCTGCTGG TTCATGCTGG TGGCCCCCAG CCCCTGCCCA
            181 TTGAGCGCCA GCTTCAGCCC TCCCTCCTGG AACAGGAGCA GCACCTCAAA GAATCTCTGG
            241 GGGTAAAAGA GGAAGGGGGC TGAGATCAGT TTCTTCTGCC CCCAGCGGGA GATCCAGGCC
            301 AGAGTTCTGT CTGCGAAGGA GGCCCTGAGT GTCACAGGAG CATGGGCAGC CTGGTCCCTC
            361 AGGCTCACAG TAAAATGCTT CGGCTCTTGC AAGACCAGTC CCCGTACTAT GATGACCTGC
            421 CCAGGCGAGA GACCCTGGGG AAGAGCATGT GAGCAGGGCA CCTCCAGCCT GGGGCTCATC
            481 AGCAGGAAAG GATGTCCAGC TGGGTACTCT CTGCTGCCCT CCACAAATGG ATTGATGTTC
            541 AGGAATCCAA CAGCCTCTAC CAGGATGTCA CCAAATATAC CCAGCGTGTC CACATGAGAC
            601 AGTGGGAGCC GGTAGCGGAA GTGGAGAAAG TGCTGTCCAT TCACACTCAC CTTCACTTCC
            661 TCATTCCCGA AGAGAAAGAG GATGAGGAAG CTGGAGCCTC TTCGCAGGGC CAGGTGGGGC
            721 CACCGGGCCT CCCTTTGCCA GCGTCCACCA TGCAGGGTGT TGCAGATGAC ATGGGGCTTG
            781 GTGGTATGGA AGCGAGGGTT GAAGTGGAAG GCGATATCTG GCCGGGGACA CAGGCTGCAG
            841 CCACACTGGA AGTCCACCTG AAACCTGTGT GCATCTAGAG GGGACCACTC CTTGCAGCAT
            901 GACCATCTTG CCTGCATGCA GGCCTCCAAA AATCGTCGTG ACATAAGGAA CCACCGGGTG
            961 GAAGACTGGT GGTTGCAGAA TGAAGCTGTC AGGAATTGGG TCCAGTTTTT CTCCAGGTGA
           1021 CATGGATCCA CGCCGGACCA GATCCGAATT TTGGAAGGAT GGGTCGCCAC CACCAACGTG
           1081 ACTTGCCAGC CTGCAAGGCA TGCTATATAC TTGCTGGATT CAGGTACTAT CAATTGTGGA
          1141 ATAGGCCTTT CATAACGTTT TTTTAGAAAC AAAACCTATA T
          //
          Primer: M13_-40
          Sequence file: RDB08428_A2Cmc.seq check
          >08428_08428_A2Cm_M13_-40_C01_07_ABI24.ab1
              1 AGGCAAGTGA TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
             61 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
            121 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
            181 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
            241 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT
            301 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT
            361 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA
            421 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA
            481 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT
            541 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT
            601 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG
            661 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA
            721 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG
            781 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT
            841 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA GGATTGTGCA
            901 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACA ACGCTGGCAC
            961 CCAGGTTGAT CGGCGCGAGA TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGCAGA
           1021 CTGAGTGGCA ACGGCCAATC AGCAACGACT GTTGGCCGCA GTTGTTTGTG CAGCGAGGGA
          1081 TGTAATCAGG TCCGCATGCC GCTTCCACTT TTCGGTTCGG AA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020).

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl